Scholarly

Evolutionary Origin of the αC Helix in Integrins

Year: 2012 Volume: 6 Issue: 5 310 - 313 Pages

Abstract: Integrins are a large family of multidomain α/β cell signaling receptors. Some integrins contain an additional inserted I domain, whose earliest expression appears to be with the chordates, since they are observed in the urochordates Ciona intestinalis (vase tunicate) and Halocynthia roretzi (sea pineapple), but not in integrins of earlier diverging species. The domain-s presence is viewed as a hallmark of integrins of higher metazoans, however in vertebrates, there are clearly three structurally-different classes: integrins without I domains, and two groups of integrins with I domains but separable by the presence or absence of an additional αC helix. For example, the αI domains in collagen-binding integrins from Osteichthyes (bony fish) and all higher vertebrates contain the specific αC helix, whereas the αI domains in non-collagen binding integrins from vertebrates and the αI domains from earlier diverging urochordate integrins, i.e. tunicates, do not. Unfortunately, within the early chordates, there is an evolutionary gap due to extinctions between the tunicates and cartilaginous fish. This, coupled with a knowledge gap due to the lack of complete genomic data from surviving species, means that the origin of collagen-binding αC-containing αI domains remains unknown. Here, we analyzed two available genomes from Callorhinchus milii (ghost shark/elephant shark; Chondrichthyes – cartilaginous fish) and Petromyzon marinus (sea lamprey; Agnathostomata), and several available Expression Sequence Tags from two Chondrichthyes species: Raja erinacea (little skate) and Squalus acanthias (dogfish shark); and Eptatretus burgeri (inshore hagfish; Agnathostomata), which evolutionary reside between the urochordates and osteichthyes. In P. marinus, we observed several fragments coding for the αC-containing αI domain, allowing us to shed more light on the evolution of the collagen-binding integrins.

Study of Environmental Effects on Sunflower Oil Percent based on Graphical Method

Year: 2012 Volume: 6 Issue: 5 306 - 309 Pages

Abstract: Biplot can be used to evaluate cultivars for their oil percent potential and stability and to evaluate trial sites for their discriminating ability and representativeness. Multi-environmental trial (MET) data for oil percent of 10 open pollinating sunflower cultivars were analyzed to investigate the genotype-environment interactions. The genotypes were evaluated in four locations with different climatic conditions in Iran in 2010. In each location, a Randomized Complete Block design with four replications was used. According to both mean and stability, Zaria, Master and R453, had highest performances among all cultivars. The graphical analysis identified best cultivar for each environment. Cultivars Berezans and Record performed best in Khoy and Islamabad. Zaria and R453 were the best genotypes in Sari and Karaj followed by Master and Favorit. The GGE bi-plot indicated two mega-environments, group one contained Karaj, Khoy and Islamabad and the second group contained Sari. The best discriminating location was Karaj followed with Khoy, Islamabad and Sari. The best representative genotypes were Zaria, R453, Master and Favorit. Ranking of ten cultivars based their oil percent was as Zaria > R453 ≈ Master ≈ Favorit > Record ≈ Berezans > Sor > Lakumka > Bulg3 > Bulg5.

Cytotaxonomy and Morphology of Chironomid Larvae (Diptera, Chironomidae) in Armenia

Year: 2012 Volume: 6 Issue: 5 301 - 305 Pages

Abstract: In the study of chironomids in Armenia several species of Orthocladiinae subfamily of Cricotopus genus, Diamesinae subfamily of Diamesa genus, and Chironominae subfamily of Chironomus genus, have been identified. In the Cricotopus genus two sibling species were found, not distinguishable by larval morphological features, but clearly distinct cytogenetically.

Detection of Pathogenic Escherichia coli Strains Pollution in Red Deer Meat in Latvia and Determination the Compatibility of VT1, VT2, eae A Genes in their Isolate

Year: 2012 Volume: 6 Issue: 5 297 - 300 Pages

Abstract: Tasks of the work were study the possible E.coli contamination in red deer meat, identify pathogenic strains from isolated E.coli, determine their incidence in red deer meat and determine the presence of VT1, VT2 and eaeA genes for the pathogenic E.coli. 8 (10%) samples were randomly selected from 80 analysed isolates of E.coli and PCR reaction was performed on them. PCR was done both on initial materials – samples of red deer meat - and for already isolated liqueurs. Two of analysed venison samples contain verotoxin-producing strains of E. coli. It means that this meat is not safe to consumer. It was proven by the sequestration reaction of E. coli and by comparison of the obtained results with the database of microorganism genome available on the internet that the isolated culture corresponds to region 16S rDNS of E. coli thus presenting correctness of the microbiological methods.

Energy Evaluation and Utilization of Cassava Peel for Lactating Dairy Cows

Year: 2012 Volume: 6 Issue: 5 294 - 296 Pages

Abstract: The experiment was then conducted to investigate the effect of cassava peel addition in the concentrate on the performance of lactating dairy cows. Twenty four Holstein Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in mid lactation; averaging 12.2+2.1 kg of milk, 119+45 days in milk, 44.1+6.2 months old and 449+33 kg live weight, were stratified for milk yield, days in milk, age, stage of lactation and body weight, and then randomly allocated to three treatment groups. The first, second and third groups were fed concentrates containing the respective cassava peel, 0, 20 and 40%. All cows were fed ad libitum corn silage and freely access to clean water. Dry matter intake, 4%FCM, milk composition and body weight change were affected (P

Enhance Halorespiration in Rhodopseudomonas palustris with Cytochrome P450cam System from Pseudomonas putida

Year: 2012 Volume: 6 Issue: 5 287 - 293 Pages

Abstract: To decompose organochlorides by bioremediation, co-culture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78% after 16 hours, however, the cytochrome P450cam system expressed strain, CGA-camCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3-chlorobenzoate, as sole carbon source or present benzoate as co-substrate, CGA-camCAB presented faster growth rate than vector control strain.

The Effects of Food Deprivation on Hematological Indices and Blood Indicators of Liver Function in Oxyleotris marmorata

Year: 2012 Volume: 6 Issue: 5 282 - 286 Pages

Abstract: Oxyleotris marmorata is considered as undomesticated fish, and its culture occasionally faces a problem of food deprivation. The present study aims to evaluate alteration of hematological indices, blood chemical associated with liver function during 4 weeks of fasting. A non-linear relationships between fasting days and hematological parameters (red blood cell number; y = - 0.002x2 + 0.041x + 1.249; R2=0.915, P0.05), mean corpuscular volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818, P>0.05 and mean corpuscular hemoglobin concentration; y = - 0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated. Significant change in hematocrit (Ht) during fasting period was observed. Ht elevated sharply increase at the first weeks of fasting period. Higher Ht also was detected during week 2-4 of fasting time. The significant reduction of hepatosomatic index was observed (y = - 0.007x2 - 0.096x + 1.414; R2=0.968, P0.05, serum glutamic oxaloacetic transaminase; y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P0.05). Taken together, prolonged fasting has deleterious effects on hematological indices, liver mass and enzyme associated in liver function. The marked adverse effects occurred after the first week of fasting state.

Recent Outbreaks of Highly Pathogenic Avian Influenza Virus in Chickens and Ducks in Egypt: Pathological Study

Year: 2012 Volume: 6 Issue: 5 273 - 281 Pages

Abstract: Clinically, chickens showed progressively developed clinical signs represented by sever cyanosis of the comb and wattles with hemorrhage at the shanks, depression, and ruffling feathers with loss of appetite and high daily mortalities. The ducks showed severe neurological signs as torticollus, seizures and inability to stand with mild signs of diarrhea and depression. Grossly, chickens showed hemorrhages and congestion in most of the organs particularly lung, liver, spleen, trachea and kidney. The examined ducks showed multiple petechial hemorrhages, multifocal hemorrhagic necrosis in the pancreas, pulmonary edema, congestion and hemorrhage in meninges and congestion in the skeletal muscles. Histopathology revealed severe congestion and hemorrhages in most of the organs particularly lung, liver and kidney. Microscopic erosive tracheitis, sever pulmonary congestion and perivascular oedema and lymphogranulocytic pneumonia were constant. The liver showed hepatocyts necrosis and lympho-granulocytic infiltration. The kidney showed renal tubular necrosis and diffuse congestion. Multifocal, neuronal necrosis, hemorrhages, multifocal glial nodules, lympho- histiocytic perivascular cuffing, and occasional neuronophagia were observed in the cerebrum. Other organs showed moderate changes.

Process Development of Safe and Ready-to-eat Raw Oyster Meat by Irradiation Technology

Year: 2012 Volume: 6 Issue: 5 268 - 272 Pages

Abstract: White scar oyster (Crassostrea belcheri) is often eaten raw and being the leading vehicle for foodborne disease, especially Salmonella Weltevreden which exposed the prominent and most resistant to radiation. Gamma irradiation at a low dose of 1 kGy was enough to eliminate S. Weltevreden contaminated in oyster meat at a level up to 5 log CFU/g while it still retain the raw characteristics and equivalent sensory quality as the non-irradiated one. Process development of ready-to-eat chilled oyster meat was conducted by shucking the meat, individually packed in plastic bags, subjected to 1 kGy gamma radiation at chilled condition and then stored in 4oC refrigerated temperature. Microbiological determination showed the absence of S. Weltevreden (5 log CFU/g initial inoculated) along the whole storage time of 30 days. Sensory evaluation indicated the decreasing in sensory scores along storage time which determining the product shelf life to be 18 days compared to 15 days of nonirradiated one. The most advantage of developed process was to provide the safe raw oyster to consumers and in addition sensory quality retained and 3-day extension shelf life also exist.

Histological Structure of the Thyroid Gland in Duck: A Light and Electron Microscopic Study

Year: 2012 Volume: 6 Issue: 5 264 - 267 Pages

Abstract: The present investigation aimed to study the histomorphometric characterizations of the thyroid gland of the duck. Five adult male and five adult female ducks were used in the experiment. Results showed that the overall histological structure of the thyroid gland of the duck were similar to those of the other vertebrae. The gland consisted of roughly spherical randomly distributed micro and macrofollicles with very little interstitial tissue between them. Each follicle is lined by a single layer of epithelial cells enclosing a cavity, the follicular cavity, which is filled with colloid. Ultrastructural findings showed that the apical surface of the follicular cells bears a variable number of short, irregularly distributed microvilli which are apparently more numerous on the columnar cells than on the lower, relatively inactive cells. Mitochondria and rough endoplasmic reticulum occupy the subnuclear region of the follicular cell, whereas the Golgi complex, free ribosomes and colloid droplets were found in the apical cytoplasm. At light or electron microscopic levels, there was no sex difference in histomorphometric characteristics of the thyroid glands.ls.

Effect of Wood Vinegar for Controlling on Housefly (Musca domestica L.)

Year: 2012 Volume: 6 Issue: 5 260 - 263 Pages

Abstract: Raw wood vinegar was purified by both standing and filtering methods. Toxicity tests were conducted under laboratory conditions by the topical application method (contact poison) and feeding method (stomach poison). Larvicidal activities of wood vinegar at four different concentrations (10, 15, 20, 25 and 30 %) were studied against second instar larvae of housefly (Musca domestica L.). Four replicates were maintained for all treatments and controls. Larval mortality was recorded up to 96 hours and compared with the larval survivability by two methods of larvicidal bioassay. Percent pupation and percent adult emergence were observed in treated M. domestica. The study revealed that the feeding method gave higher efficiency compared with the topical application method. Larval mortality increased with increasing concentration of wood vinegar and the duration of exposure. No mortality was found in treated M. domestica larvae at minimum 10% concentration of wood vinegar through the experiments. The treated larvae were maintained up to pupa and adult emergence. At 30% maximum concentration larval duration was extended to 11 days in M. domestica for topical application method and 9 days for feeding method. Similarly the pupal durations were also increased with increased concentrations (16 and 24 days for topical application method and feeding method respectively at 30% concentration) of the treatments.

Sex Differences in Thyroid Gland Structure of Rabbits

Year: 2012 Volume: 6 Issue: 5 256 - 259 Pages

Abstract: The aim of the present investigation was to compare sex differences in thyroid gland structure of rabbits. Five adult male and five adult female (3.1-3.5 kg body weight) New Zealand white rabbits were used in the experiment. Results showed that at light microscopic level, there was no sex difference in microscopic appearance of the thyroid glands. At electron microscopic level, however, the mitochondria and the microvilli of the follicular cells are more numerous and the Golgi complex is also more extensive in male rabbits in comparison to females. Results obtained from micrometric measurements showed that the volume density of the follicles is higher in males than in females, but the differences are not statistically significant .The volume density of epithelium and the height of follicular cells are significantly greater in males than in females and reverse is true about the volume density of interstitium (p

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