Ultrasensitive Hepatitis B Virus Detection in Blood Using Nano-Porous Silicon Oxide: Towards POC Diagnostics
Early diagnosis of infection like Hep-B virus in blood
is important for low cost medical treatment. For this purpose, it is
desirable to develop a point of care device which should be able to
detect trace quantities of the target molecule in blood. In this paper,
we report a nanoporous silicon oxide sensor which is capable of
detecting down to 1fM concentration of Hep-B surface antigen in
blood without the requirement of any centrifuge or pre-concentration.
This has been made possible by the presence of resonant peak in the
sensitivity characteristics. This peak is observed to be dependent only
on the concentration of the specific antigen and not on the interfering
species in blood serum. The occurrence of opposite impedance
change within the pores and at the bottom of the pore is responsible
for this effect. An electronic interface has also been designed to
provide a display of the virus concentration.
[1] Li Mei, Peng Wu, Jing Ye, Guangping Gao, Lin Shao, Shaomei Huang,
Yaoming Li, Xiaohong Yang, Huanchun Chen, and Shengbo Cao,
“Development and application of an antigen capture ELISA assay for
diagnosis of Japanese encephalitis virus in swine, human and mosquito,”
Virology Journal , vol. 9: 4, 2012.
[2] Xue-Yong Huang, Xiao-Ning Hu, Hong Ma, Yan-Hua Du, Hong-Xia
Ma, Kai Kang, Ai-Guo You, Hai-Feng Wang, Li Zhang, Hao-Min Chen,
J. Stephen Dumler, and Bian-Li Xu, “Detection of New Bunyavirus
RNA by Reverse Transcription–Loop-Mediated Isothermal
Amplification,” J. clinical microbiology, vol. 52, 2014, pp. 531-535.
[3] Yong-Hwan Choi, Ga-Yeon Lee, Hyuk Ko, Young Wook Chang, Min-
Jung Kang, and Jae-Chul Pyun, “Development of SPR biosensor for the
detection of human hepatitis B virus using plasma-treated parylene-N
film,” Biosens. Bioelectron., vol. 56, 2014, pp. 286–294.
[4] Cuixia Ma, Mo Liang, Li Wang, Hua Xiang, Yingtao Jiang, Yiyan Li,
and Guoming Xie, “MultisHRP-DNA-coated CMWNTs as signal labels
for an ultrasensitive hepatitis C virus core antigen electrochemical
immunosensor,” Biosens. Bioelectron., Vol. 47, 2013, pp. 467–474.
[5] Jee-Yeon Kim, Jae-Hyuk Ahn, Dong-II Moon, Tae Jung Park, Sang Yup
Lee, and Yang-Kyu Choi, “Multiplex electrical detection of avian
influenza and human immunodeficiency virus with an underlapembedded
silicon nanowire field-effect transistor,” Biosens.
Bioelectron., vol. 55, 2014, pp. 162–167.
[6] Dawid Nidzworski, Paulina Pranszke, Magda Grudniewska, Ewelina
Król, and Beata Gromadzka, “Universal biosensor for detection of
influenza virus,” Biosens. Bioelectron., vol. 59, 2014, pp. 239–242.
[7] Thi Thanh Binh Nguyen, Guiwan Koh, Hui Si Lim, Anthony JS Chua,
Mary M L Ng, and Chee Seng Toh, “Membrane-Based Electrochemical
Nanobiosensor for the Detection of Virus,” Anal. Chem., vol. 81, 2009,
pp. 7226–7234.
[8] Jiajia Deng and Chee-Seng Toh, “Impedimetric DNA Biosensor Based
on a Nanoporous Alumina Membrane for the Detection of the Specific
Oligonucleotide Sequence of Dengue Virus,” Sensors, vol. 13, 2013, pp.
7774-7785.
[9] Gengfeng Zheng, Xuan P. A. Gao, and Charles M. Lieber, “Frequency
Domain Detection of Biomolecules Using Silicon Nanowire
Biosensors,” NanoLetts., vol. 10, 2010, pp. 3179–3183.
[10] Roberto de la Rica and Molly M. Stevens, “Plasmonic ELISA for the
ultrasensitive detection of disease biomarkers with the naked eye,” Nat.
Nanotech., vol. 7, 2012, pp. 821–824. [11] H. Ghosh and C. RoyChaudhuri, “Ultrasensitive food toxin biosensor
using frequency based signals of silicon oxide nanoporous structure,”
Appl. Phys. Lett., Vol. 102, 2013, pp. 243701.
[12] R. Dev Das, S. Maji, S. Das, and C. RoyChaudhuri, “Optimization of
covalent antibody immobilization on macroporous silicon solid
supports,” Appl. Surface Sci., vol. 256, 2010, pp.5867-575.
[1] Li Mei, Peng Wu, Jing Ye, Guangping Gao, Lin Shao, Shaomei Huang,
Yaoming Li, Xiaohong Yang, Huanchun Chen, and Shengbo Cao,
“Development and application of an antigen capture ELISA assay for
diagnosis of Japanese encephalitis virus in swine, human and mosquito,”
Virology Journal , vol. 9: 4, 2012.
[2] Xue-Yong Huang, Xiao-Ning Hu, Hong Ma, Yan-Hua Du, Hong-Xia
Ma, Kai Kang, Ai-Guo You, Hai-Feng Wang, Li Zhang, Hao-Min Chen,
J. Stephen Dumler, and Bian-Li Xu, “Detection of New Bunyavirus
RNA by Reverse Transcription–Loop-Mediated Isothermal
Amplification,” J. clinical microbiology, vol. 52, 2014, pp. 531-535.
[3] Yong-Hwan Choi, Ga-Yeon Lee, Hyuk Ko, Young Wook Chang, Min-
Jung Kang, and Jae-Chul Pyun, “Development of SPR biosensor for the
detection of human hepatitis B virus using plasma-treated parylene-N
film,” Biosens. Bioelectron., vol. 56, 2014, pp. 286–294.
[4] Cuixia Ma, Mo Liang, Li Wang, Hua Xiang, Yingtao Jiang, Yiyan Li,
and Guoming Xie, “MultisHRP-DNA-coated CMWNTs as signal labels
for an ultrasensitive hepatitis C virus core antigen electrochemical
immunosensor,” Biosens. Bioelectron., Vol. 47, 2013, pp. 467–474.
[5] Jee-Yeon Kim, Jae-Hyuk Ahn, Dong-II Moon, Tae Jung Park, Sang Yup
Lee, and Yang-Kyu Choi, “Multiplex electrical detection of avian
influenza and human immunodeficiency virus with an underlapembedded
silicon nanowire field-effect transistor,” Biosens.
Bioelectron., vol. 55, 2014, pp. 162–167.
[6] Dawid Nidzworski, Paulina Pranszke, Magda Grudniewska, Ewelina
Król, and Beata Gromadzka, “Universal biosensor for detection of
influenza virus,” Biosens. Bioelectron., vol. 59, 2014, pp. 239–242.
[7] Thi Thanh Binh Nguyen, Guiwan Koh, Hui Si Lim, Anthony JS Chua,
Mary M L Ng, and Chee Seng Toh, “Membrane-Based Electrochemical
Nanobiosensor for the Detection of Virus,” Anal. Chem., vol. 81, 2009,
pp. 7226–7234.
[8] Jiajia Deng and Chee-Seng Toh, “Impedimetric DNA Biosensor Based
on a Nanoporous Alumina Membrane for the Detection of the Specific
Oligonucleotide Sequence of Dengue Virus,” Sensors, vol. 13, 2013, pp.
7774-7785.
[9] Gengfeng Zheng, Xuan P. A. Gao, and Charles M. Lieber, “Frequency
Domain Detection of Biomolecules Using Silicon Nanowire
Biosensors,” NanoLetts., vol. 10, 2010, pp. 3179–3183.
[10] Roberto de la Rica and Molly M. Stevens, “Plasmonic ELISA for the
ultrasensitive detection of disease biomarkers with the naked eye,” Nat.
Nanotech., vol. 7, 2012, pp. 821–824. [11] H. Ghosh and C. RoyChaudhuri, “Ultrasensitive food toxin biosensor
using frequency based signals of silicon oxide nanoporous structure,”
Appl. Phys. Lett., Vol. 102, 2013, pp. 243701.
[12] R. Dev Das, S. Maji, S. Das, and C. RoyChaudhuri, “Optimization of
covalent antibody immobilization on macroporous silicon solid
supports,” Appl. Surface Sci., vol. 256, 2010, pp.5867-575.
@article{"International Journal of Medical, Medicine and Health Sciences:69777", author = "N. Das and N. Samanta and L. Pandey and C. Roy Chaudhuri", title = "Ultrasensitive Hepatitis B Virus Detection in Blood Using Nano-Porous Silicon Oxide: Towards POC Diagnostics", abstract = "Early diagnosis of infection like Hep-B virus in blood
is important for low cost medical treatment. For this purpose, it is
desirable to develop a point of care device which should be able to
detect trace quantities of the target molecule in blood. In this paper,
we report a nanoporous silicon oxide sensor which is capable of
detecting down to 1fM concentration of Hep-B surface antigen in
blood without the requirement of any centrifuge or pre-concentration.
This has been made possible by the presence of resonant peak in the
sensitivity characteristics. This peak is observed to be dependent only
on the concentration of the specific antigen and not on the interfering
species in blood serum. The occurrence of opposite impedance
change within the pores and at the bottom of the pore is responsible
for this effect. An electronic interface has also been designed to
provide a display of the virus concentration.
", keywords = "Impedance spectroscopy, Ultrasensitive detection in
blood, Peak frequency, Electronic interface.", volume = "9", number = "5", pages = "387-5", }
