Phase Equilibrium in Aqueous Two-phase Systems Containing Poly (propylene glycol) and Sodium Citrate at Different pH
The phase diagrams and compositions of coexisting
phases have been determined for aqueous two-phase systems
containing poly(propylene glycol) with average molecular weight of
425 and sodium citrate at various pH of 3.93, 4.44, 4.6, 4.97, 5.1,
8.22. The effect of pH on the salting-out effect of poly (propylene
glycol) by sodium citrate has been studied. It was found that, an
increasing in pH caused the expansion of two-phase region.
Increasing pH also increases the concentration of PPG in the PPGrich
phase, while the salt-rich phase will be somewhat mole diluted.
[1] P.A. Albertsson, Partitioning of Cell Particles and Macromolecules, 3rd
ed., Wiley-Interscience, New York, 1986.
[2] H.Walter, D.E. Brooks, D. Fisher, Partitioning in Aqueous Two-Phase
Systems, Academic Press, New York, 1985.
[3] J.G. Huddleston, H.D. Willauer, K.R. Boaz, R.B. Rogers, "Separation
and recovery of food coloring dyes using aqueous biphasic extraction
chromatographic resins", J. Chromatogr. B, vol. 711, pp. 237-244,
1998.
[4] R. Hatti-Kaul, Aqueous Two Phase Systems: Methods and Protocols,
Humanae Press, New Jersey, 2000.
[5] R.B. Rogers, H.D. Willauer, S.T. Griffin, J.G. Huddleston, "Partitioning
of small organic molecules in aqueous biphasic systems", J.
Chromatogr. B,, vol. 711, pp. 255-263, 1998.
[6] Zafarani-Moattar, M.T., Salabat, A., "Thermodynamics of magnesium
sulfate-polypropylene glycol aqueous two-phase system. Experiment
and correlation", Fluid Phase Equilib., vol. 152, pp. 57-65, 1998.
[7] Zafarani-Moattar, M.T., Sadeghi, R., "Phase diagram Data for Several
PPG + Salt Aqueous Biphasic Systems at 25 ┬░C, J. Chem. Eng. Data,
vol. 50, pp. 947-950, 2000.
[8] Hustedt, H. "Extractive Enzyme Recovery with Simple Recycling of
Phase Forming Chemicals", Biotechnol. Lett., vol. 8, pp. 791-796,
1986.
[9] Greve, A.; Kula, M. R., "Recycling of salt from the primary bottom
phase of protein extraction process", J. Chem. Technol. Biotechnol.,
vol. 50, pp. 27-42, 1991.
[10] Vernau, J.; Kula, M. R. "Extraction of Proteins from Biological Raw
Material Using Aqueous Poly(ethylene) glycol-Citrate Phase Systems",
Biotechnol. Appl. Biochem., vol. 12, pp. 397-404, 1990.
[11] F. Rahimpour., F. Feyzi., S. Maghsodi and R. H. Kaul, "Purification of
plasmid DNA with polymer-Salt aqueous two-phase system:
optimization using response surface methodology", Biotechnol. Bioeng.,
vol. 95, pp 627-637, 2006.
[12] Waziri, S.M., Abu-Sharkh, B.F., Ali, S.A., "The effect of pH and salt
concentration on the coexistence curves of aqueous two-phase systems
containing a pH responsive copolymer and polyethylene glycol", Fluid
Phase Equilib., vol. 205 pp. 275-290, 2003.
[1] P.A. Albertsson, Partitioning of Cell Particles and Macromolecules, 3rd
ed., Wiley-Interscience, New York, 1986.
[2] H.Walter, D.E. Brooks, D. Fisher, Partitioning in Aqueous Two-Phase
Systems, Academic Press, New York, 1985.
[3] J.G. Huddleston, H.D. Willauer, K.R. Boaz, R.B. Rogers, "Separation
and recovery of food coloring dyes using aqueous biphasic extraction
chromatographic resins", J. Chromatogr. B, vol. 711, pp. 237-244,
1998.
[4] R. Hatti-Kaul, Aqueous Two Phase Systems: Methods and Protocols,
Humanae Press, New Jersey, 2000.
[5] R.B. Rogers, H.D. Willauer, S.T. Griffin, J.G. Huddleston, "Partitioning
of small organic molecules in aqueous biphasic systems", J.
Chromatogr. B,, vol. 711, pp. 255-263, 1998.
[6] Zafarani-Moattar, M.T., Salabat, A., "Thermodynamics of magnesium
sulfate-polypropylene glycol aqueous two-phase system. Experiment
and correlation", Fluid Phase Equilib., vol. 152, pp. 57-65, 1998.
[7] Zafarani-Moattar, M.T., Sadeghi, R., "Phase diagram Data for Several
PPG + Salt Aqueous Biphasic Systems at 25 ┬░C, J. Chem. Eng. Data,
vol. 50, pp. 947-950, 2000.
[8] Hustedt, H. "Extractive Enzyme Recovery with Simple Recycling of
Phase Forming Chemicals", Biotechnol. Lett., vol. 8, pp. 791-796,
1986.
[9] Greve, A.; Kula, M. R., "Recycling of salt from the primary bottom
phase of protein extraction process", J. Chem. Technol. Biotechnol.,
vol. 50, pp. 27-42, 1991.
[10] Vernau, J.; Kula, M. R. "Extraction of Proteins from Biological Raw
Material Using Aqueous Poly(ethylene) glycol-Citrate Phase Systems",
Biotechnol. Appl. Biochem., vol. 12, pp. 397-404, 1990.
[11] F. Rahimpour., F. Feyzi., S. Maghsodi and R. H. Kaul, "Purification of
plasmid DNA with polymer-Salt aqueous two-phase system:
optimization using response surface methodology", Biotechnol. Bioeng.,
vol. 95, pp 627-637, 2006.
[12] Waziri, S.M., Abu-Sharkh, B.F., Ali, S.A., "The effect of pH and salt
concentration on the coexistence curves of aqueous two-phase systems
containing a pH responsive copolymer and polyethylene glycol", Fluid
Phase Equilib., vol. 205 pp. 275-290, 2003.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:53506", author = "Farshad Rahimpour and Ali Reza Baharvand", title = "Phase Equilibrium in Aqueous Two-phase Systems Containing Poly (propylene glycol) and Sodium Citrate at Different pH", abstract = "The phase diagrams and compositions of coexisting
phases have been determined for aqueous two-phase systems
containing poly(propylene glycol) with average molecular weight of
425 and sodium citrate at various pH of 3.93, 4.44, 4.6, 4.97, 5.1,
8.22. The effect of pH on the salting-out effect of poly (propylene
glycol) by sodium citrate has been studied. It was found that, an
increasing in pH caused the expansion of two-phase region.
Increasing pH also increases the concentration of PPG in the PPGrich
phase, while the salt-rich phase will be somewhat mole diluted.", keywords = "Aqueous two-phase system, Phase equilibrium,Biomolecules purification", volume = "3", number = "11", pages = "611-4", }
