Long-Term Treatment of Puerariae Radix Extract Ameliorated Hyperparathyroidism Induced by Ovariectomy in Mature Female Rats
Postmenopausal osteoporosis is a disorder
characterized by the progressive bone loss induced by estrogen
deficiency in postmenopausal women. This imbalance affects
calcium–phosphate metabolism and results in secondary
hyperparathyroidism. Purariae Radix (PR), the root of P. lobata
(Wild.) Ohwi, is one of the earliest medicinal herbs employed in
ancient China. PR contains a high quantity of isoflavones and their
glycosides, which are regarded as phytoestrogen. Few investigations
of PR are related to its osteoprotective effects. The present study is
designed to administer PR water extract to ovariectomized (OVX)
female rats, for the investigation of its possibly protective actions on
bone and to delineate the potential mechanisms involved. Our results
demonstrated that long-term treatment of PR could not significantly
improve bone properties, whereas it greatly ameliorated the condition
of secondary hyperparathyroidism induced by ovariectomy in those
animals. PR might be useful as alternative regimen for protecting
against postmenopausal bone loss.
[1] R. Pacifici, "Cytokines, estrogen, and postmenopausal osteoporosis--the
second decade", Endocrinology, vol. 139(6), pp. 2659-2661, 1998.
[2] L.G. Raisz, "Pathogenesis of osteoporosis: concepts, conflicts, and
prospects", J Clin Invest, vol. 115(12), pp. 3318-3325, 2005.
[3] S.C. Manolagas, "Birth and death of bone cells: basic regulatory
mechanisms and implications for the pathogenesis and treatment of
osteoporosis", Endocr Rev, vol. 21(2), pp. 115-137, 2002.
[4] S. Khosla, E.J. Atkinson, L.J. Melton and B.L. Riggs, "Effects of age and
estrogen status on serum parathyroid hormone levels and biochemical
markers of bone turnover in women: a population-based study", J Clin
Endocrinol Metab, vol. 82(5), pp. 1522-1527, 1997.
[5] W.R. McKane, S. Khosla, J. Risteli, S.P. Robins, J.M. Muhs and B.L.
Riggs, "Role of estrogen deficiency in pathogenesis of secondary
hyperparathyroidism and increased bone resorption in elderly women",
Proc Assoc Am Physicians, vol. 109(2), pp. 174-180, 1997.
[6] J.L. Stock, J.A. Coderre and L.E. Mallette, "Effects of a short course of
estrogen on mineral metabolism in postmenopausal women", J Clin
Endocrinol Metab, vol. 61(4), pp. 595-600, 1985.
[7] J. Mei, S.S. Yeung and A.W. Kung, "High dietary phytoestrogen intake is
associated with higher bone mineral density in postmenopausal but not
premenopausal women", J Clin Endocrinol Meta, vol. 86(11), pp.
5217-5221, 2001.
[8] X. Wang, J. Wu, H. Chiba, K. Umegaki, K. Yamada and Y. Ishimi,
"Puerariae radix prevents bone loss in ovariectomized mice", J Bone
Miner Metab, vol. 21(5), pp. 268-275, 2003.
[9] X. Wang, J. Wu, H. Chiba, K. Umegaki, K. Yamada and Y. Ishimi,
"Puerariae radix prevents bone loss in castrated male mice", Metabolism,
vol. 54(11), pp. 1536-1541, 2005.
[10] K.H. Wong, G.Q. Li, K.M. Li, V. Razmovski-Naumovski and K. Chan,
"Kudzu root: traditional uses and potential medicinal benefits in diabetes
and cardiovascular diseases", J Ethnopharmacol, vol. 134(3), pp.
584-607, 2011.
[11] J.K. Prasain, K. Jones, M. Kirk, L. Wilson, M. Smith-Johnson, C.
Weaver and S. Barnes, "Profiling and quantification of isoflavonoids in
kudzu dietary supplements by high-performance liquid chromatography
and electrospray ionization tandem mass spectrometry", J Agric Food
Chem, vol. 51(15), pp. 4213-4218, 2003.
[12] L.L. Legette, W.H. Lee, B.R. Martin, J.A. Story, A. Arabshahi, S. Barnes
and C.M. Weaver, "Genistein, a phytoestrogen, improves total
cholesterol, and Synergy, a prebiotic, improves calcium utilization, but
there were no synergistic effects", Menopause, vol. 18(8), pp. 923-931.
[13] Y. Zhang, W.P. Lai, P.C. Leung, C.F. Wu, X.S. Yao and M.S. Wong,
"Effects of Fructus Ligustri Lucidi extract on bone turnover and calcium
balance in ovariectomized rats", Biol Pharm Bull, vol. 29(2), pp. 291-296,
2006.
[14] Y. Zhang, W.P. Lai, C.F. Wu, M.J. Favus, P.C. Leung and M.S. Wong,
"Ovariectomy worsens secondary hyperparathyroidism in mature rats
during low-Ca diet", Am J Physiol Endocrinol Metab, vol. 292(3), pp.
E723-731, 2007.
[15] H. Trisomboon, S. Malaivijitnond, J. Suzuki, Y. Hamada, G. Watanabe
and K. Taya, "Long-term treatment effects of Pueraria mirifica
phytoestrogens on parathyroid hormone and calcium levels in aged
menopausal cynomolgus monkeys", J Reprod Dev, vol. 50(6), pp.
639-645, 2004.
[16] H. Trisomboon, S. Malaivijitnond, J. Suzuki, Y. Hamada, G. Watanabe
and K. Taya, " Long-term treatment effects of Pueraria mirifica
phytoestrogens on parathyroid hormone and calcium levels in aged
menopausal cynomolgus monkeys", J Repro Dev, vol. 50, pp. 639-645,
2004.
[1] R. Pacifici, "Cytokines, estrogen, and postmenopausal osteoporosis--the
second decade", Endocrinology, vol. 139(6), pp. 2659-2661, 1998.
[2] L.G. Raisz, "Pathogenesis of osteoporosis: concepts, conflicts, and
prospects", J Clin Invest, vol. 115(12), pp. 3318-3325, 2005.
[3] S.C. Manolagas, "Birth and death of bone cells: basic regulatory
mechanisms and implications for the pathogenesis and treatment of
osteoporosis", Endocr Rev, vol. 21(2), pp. 115-137, 2002.
[4] S. Khosla, E.J. Atkinson, L.J. Melton and B.L. Riggs, "Effects of age and
estrogen status on serum parathyroid hormone levels and biochemical
markers of bone turnover in women: a population-based study", J Clin
Endocrinol Metab, vol. 82(5), pp. 1522-1527, 1997.
[5] W.R. McKane, S. Khosla, J. Risteli, S.P. Robins, J.M. Muhs and B.L.
Riggs, "Role of estrogen deficiency in pathogenesis of secondary
hyperparathyroidism and increased bone resorption in elderly women",
Proc Assoc Am Physicians, vol. 109(2), pp. 174-180, 1997.
[6] J.L. Stock, J.A. Coderre and L.E. Mallette, "Effects of a short course of
estrogen on mineral metabolism in postmenopausal women", J Clin
Endocrinol Metab, vol. 61(4), pp. 595-600, 1985.
[7] J. Mei, S.S. Yeung and A.W. Kung, "High dietary phytoestrogen intake is
associated with higher bone mineral density in postmenopausal but not
premenopausal women", J Clin Endocrinol Meta, vol. 86(11), pp.
5217-5221, 2001.
[8] X. Wang, J. Wu, H. Chiba, K. Umegaki, K. Yamada and Y. Ishimi,
"Puerariae radix prevents bone loss in ovariectomized mice", J Bone
Miner Metab, vol. 21(5), pp. 268-275, 2003.
[9] X. Wang, J. Wu, H. Chiba, K. Umegaki, K. Yamada and Y. Ishimi,
"Puerariae radix prevents bone loss in castrated male mice", Metabolism,
vol. 54(11), pp. 1536-1541, 2005.
[10] K.H. Wong, G.Q. Li, K.M. Li, V. Razmovski-Naumovski and K. Chan,
"Kudzu root: traditional uses and potential medicinal benefits in diabetes
and cardiovascular diseases", J Ethnopharmacol, vol. 134(3), pp.
584-607, 2011.
[11] J.K. Prasain, K. Jones, M. Kirk, L. Wilson, M. Smith-Johnson, C.
Weaver and S. Barnes, "Profiling and quantification of isoflavonoids in
kudzu dietary supplements by high-performance liquid chromatography
and electrospray ionization tandem mass spectrometry", J Agric Food
Chem, vol. 51(15), pp. 4213-4218, 2003.
[12] L.L. Legette, W.H. Lee, B.R. Martin, J.A. Story, A. Arabshahi, S. Barnes
and C.M. Weaver, "Genistein, a phytoestrogen, improves total
cholesterol, and Synergy, a prebiotic, improves calcium utilization, but
there were no synergistic effects", Menopause, vol. 18(8), pp. 923-931.
[13] Y. Zhang, W.P. Lai, P.C. Leung, C.F. Wu, X.S. Yao and M.S. Wong,
"Effects of Fructus Ligustri Lucidi extract on bone turnover and calcium
balance in ovariectomized rats", Biol Pharm Bull, vol. 29(2), pp. 291-296,
2006.
[14] Y. Zhang, W.P. Lai, C.F. Wu, M.J. Favus, P.C. Leung and M.S. Wong,
"Ovariectomy worsens secondary hyperparathyroidism in mature rats
during low-Ca diet", Am J Physiol Endocrinol Metab, vol. 292(3), pp.
E723-731, 2007.
[15] H. Trisomboon, S. Malaivijitnond, J. Suzuki, Y. Hamada, G. Watanabe
and K. Taya, "Long-term treatment effects of Pueraria mirifica
phytoestrogens on parathyroid hormone and calcium levels in aged
menopausal cynomolgus monkeys", J Reprod Dev, vol. 50(6), pp.
639-645, 2004.
[16] H. Trisomboon, S. Malaivijitnond, J. Suzuki, Y. Hamada, G. Watanabe
and K. Taya, " Long-term treatment effects of Pueraria mirifica
phytoestrogens on parathyroid hormone and calcium levels in aged
menopausal cynomolgus monkeys", J Repro Dev, vol. 50, pp. 639-645,
2004.
@article{"International Journal of Biological, Life and Agricultural Sciences:58781", author = "Xiao-Li Dong and Quan-Gui Gao and Sa-Sa Gu and Hao-Tian Feng and Man-Sau Wong and Liya Denney", title = "Long-Term Treatment of Puerariae Radix Extract Ameliorated Hyperparathyroidism Induced by Ovariectomy in Mature Female Rats", abstract = "Postmenopausal osteoporosis is a disorder
characterized by the progressive bone loss induced by estrogen
deficiency in postmenopausal women. This imbalance affects
calcium–phosphate metabolism and results in secondary
hyperparathyroidism. Purariae Radix (PR), the root of P. lobata
(Wild.) Ohwi, is one of the earliest medicinal herbs employed in
ancient China. PR contains a high quantity of isoflavones and their
glycosides, which are regarded as phytoestrogen. Few investigations
of PR are related to its osteoprotective effects. The present study is
designed to administer PR water extract to ovariectomized (OVX)
female rats, for the investigation of its possibly protective actions on
bone and to delineate the potential mechanisms involved. Our results
demonstrated that long-term treatment of PR could not significantly
improve bone properties, whereas it greatly ameliorated the condition
of secondary hyperparathyroidism induced by ovariectomy in those
animals. PR might be useful as alternative regimen for protecting
against postmenopausal bone loss.", keywords = "Hyperparathyroidism, Ovariectomy, Postmenopausal
Osteoporosis, Purariae Radix", volume = "6", number = "9", pages = "718-5", }
