Inhibition Effect of Brazilin to Human Bladder Cancer Cell Line T24
The inhibition effect of brazilin to human bladder
tumor cell line T24 in vitro and in vivo was studied. The results of the
in vitro experiments showed that brazilin has strong inhibition activity
on the target cells. The inhibition ratio of 100 μg/mL brazilin and 100
μg/mL mitomycin to the target cells was 90.90 % and 63.24 %
respectively, which showed that brazilin has higher inhibition activity
than mitomycin under the same concentration. Brazilin could induce
cell apoptosis in T24 cells. Significant antitumor activity of brazilin
was also showed in the animals experiments. The life extention rate of
200 mg/mL, 300 mg/kg, and 400 mg/kg brazilin intraperitoneally
injected into Balb/c-nu-nu nude mice that with human bladder cancer
were 51.50 %, 56.90 %, and 58.42 %（P<0.05）. Our study showed that
brazilin has significant inhibitory effect on human bladder tumor cell.
[1] XM Zhang, GP Wang, LS Ren. Study of Caesalpinia Sppan aqueous
extract-s apoptosis-inducing effects on human ovarian cancer cell
SKOV3. Cancer Research and Clinic 2010; 22: 388-390.
[2] XM Zhang, GP Wang, LS Ren. Caesalpinia Sppan aqueous extract-s
growth inhibiting and apoptosis-inducing effects on human ovarian
cancer cell. Cancer Research and Clinic 2010; 22: 243-246.
[3] LS Ren, H Zhang, XH Bai, XB Han, ZG Mi. Hematoxylin-s cytocidal and
apoptosis effects on human urinary cancer cell-T24. Cancer Research
and Clinic 2008; 20: 799-801.
[4] AL Liu , SH Shu , HL Qin , SM Lee , YT Wang , GH Du. In vitro
anti-influenza viral activities of constituents from Caesalpinia sappan.
Planta Med 2009; 75: 337-339.
[5] CM Hu , YH Liu , KP Cheah , JS Li , CS Lam , WY Yu , CS Choy . Heme
oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7
macrophages stimulated with lipopolysaccharide. J Ethnopharmacol
2009; 121: 79-85.
[6] IK Bae , HY Min , AR Han , EK Seo , SK Lee . Suppression of
lipopolysaccharide-induced expression of inducible nitric oxide synthase
by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol 2005; 513
: 237-242.
[7] CK Moon , KS Park , SG Kim , HS Won , JH Chung . Brazilin protects
cultured rat hepatocytes from BrCCl3-induced toxicity. Drug Chem
Toxicol 1992; 15: 81-91.
[8] LY Khil , SS Han , SG Kim , TS Chang , SD Jeon , DS So , CK Moon .
Effects of brazilin on GLUT4 recruitment in isolated rat epididymal
adipocytes. Biochem Pharmacol 1999; 58: 1705-1712.
[9] HS Won , J Lee , LY Khil, SH Chae , MY Ahn , BH Lee , JH Chung , YC
Kim , CK Moon . Mechanism of action of Brazilin on gluconeogenesis in
isolated rat hepatocytes. Planta Med 2004; 70: 740-744.
[10] EJ You , LY Khil , WJ Kwak , HS Won , SH Chae , BH Lee , CK Moon .
Effects of brazilin on the production of fructose-2,6- bisphosphate in rat
hepatocytes. J Ethnopharmacol 2005; 102(1): 53-57.
[11] HX Xu , SF Lee . The antibacterial principle of Caesalpina sappan.
Phytother Res 2004; 18: 647-651.
[12] CM Hu , JJ Kang , CC Lee , CH Li, JW Liao , YW Cheng . Induction of
vasorelaxation through activation of nitric oxide synthase in endothelial
cells by brazilin. Eur J Pharmacol 2003; 468: 37-45.
[13] YW Xie , DS Ming , HX Xu , H Dong , PP But . Vasorelaxing effects of
Caesalpinia sappan involvement of endogenous nitric oxide. Life Sci
2000; 67: 1913-1918.
[14] GS Hwang , JY Kim , TS Chang , SD Jeon , DS So , CK Moon . Effects of
Brazilin on the phospholipase A2 activity and changes of intracellular
free calcium concentration in rat platelets. Arch Pharm Res 1998; 21:
774-778.
[15] KM Yang , SD Jeon , DS So , Moon CK. Brazilin augments cellular
immunity in multiple low dose streptozotocin (MLD-STZ) induced type I
diabetic mice. Arch Pharm Res 2000 ; 23: 626-632.
[16] MS Mok , SD Jeon , KM Yang , DS So , CK Moon . Effects of Brazilin on
induction of immunological tolerance by sheep red blood cells in
C57BL/6 female mice. Arch Pharm Res 1998; 21: 769-773.
[17] SY Choi , KM Yang , SD Jeon, JH Kim , LY Khil , TS Chang , Moon CK.
Brazilin modulates immune function mainly by augmenting T cell
activity in halothane administered mice. Planta Med 1997; 63: 405-408.
[18] W Mar , HT Lee , KH Je , HY Choi , EK Seo . A DNA strand-nicking
principle of a higher plant, Caesalpinia sappan. Arch Pharm Res 2003; 26:
147-150
[1] XM Zhang, GP Wang, LS Ren. Study of Caesalpinia Sppan aqueous
extract-s apoptosis-inducing effects on human ovarian cancer cell
SKOV3. Cancer Research and Clinic 2010; 22: 388-390.
[2] XM Zhang, GP Wang, LS Ren. Caesalpinia Sppan aqueous extract-s
growth inhibiting and apoptosis-inducing effects on human ovarian
cancer cell. Cancer Research and Clinic 2010; 22: 243-246.
[3] LS Ren, H Zhang, XH Bai, XB Han, ZG Mi. Hematoxylin-s cytocidal and
apoptosis effects on human urinary cancer cell-T24. Cancer Research
and Clinic 2008; 20: 799-801.
[4] AL Liu , SH Shu , HL Qin , SM Lee , YT Wang , GH Du. In vitro
anti-influenza viral activities of constituents from Caesalpinia sappan.
Planta Med 2009; 75: 337-339.
[5] CM Hu , YH Liu , KP Cheah , JS Li , CS Lam , WY Yu , CS Choy . Heme
oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7
macrophages stimulated with lipopolysaccharide. J Ethnopharmacol
2009; 121: 79-85.
[6] IK Bae , HY Min , AR Han , EK Seo , SK Lee . Suppression of
lipopolysaccharide-induced expression of inducible nitric oxide synthase
by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol 2005; 513
: 237-242.
[7] CK Moon , KS Park , SG Kim , HS Won , JH Chung . Brazilin protects
cultured rat hepatocytes from BrCCl3-induced toxicity. Drug Chem
Toxicol 1992; 15: 81-91.
[8] LY Khil , SS Han , SG Kim , TS Chang , SD Jeon , DS So , CK Moon .
Effects of brazilin on GLUT4 recruitment in isolated rat epididymal
adipocytes. Biochem Pharmacol 1999; 58: 1705-1712.
[9] HS Won , J Lee , LY Khil, SH Chae , MY Ahn , BH Lee , JH Chung , YC
Kim , CK Moon . Mechanism of action of Brazilin on gluconeogenesis in
isolated rat hepatocytes. Planta Med 2004; 70: 740-744.
[10] EJ You , LY Khil , WJ Kwak , HS Won , SH Chae , BH Lee , CK Moon .
Effects of brazilin on the production of fructose-2,6- bisphosphate in rat
hepatocytes. J Ethnopharmacol 2005; 102(1): 53-57.
[11] HX Xu , SF Lee . The antibacterial principle of Caesalpina sappan.
Phytother Res 2004; 18: 647-651.
[12] CM Hu , JJ Kang , CC Lee , CH Li, JW Liao , YW Cheng . Induction of
vasorelaxation through activation of nitric oxide synthase in endothelial
cells by brazilin. Eur J Pharmacol 2003; 468: 37-45.
[13] YW Xie , DS Ming , HX Xu , H Dong , PP But . Vasorelaxing effects of
Caesalpinia sappan involvement of endogenous nitric oxide. Life Sci
2000; 67: 1913-1918.
[14] GS Hwang , JY Kim , TS Chang , SD Jeon , DS So , CK Moon . Effects of
Brazilin on the phospholipase A2 activity and changes of intracellular
free calcium concentration in rat platelets. Arch Pharm Res 1998; 21:
774-778.
[15] KM Yang , SD Jeon , DS So , Moon CK. Brazilin augments cellular
immunity in multiple low dose streptozotocin (MLD-STZ) induced type I
diabetic mice. Arch Pharm Res 2000 ; 23: 626-632.
[16] MS Mok , SD Jeon , KM Yang , DS So , CK Moon . Effects of Brazilin on
induction of immunological tolerance by sheep red blood cells in
C57BL/6 female mice. Arch Pharm Res 1998; 21: 769-773.
[17] SY Choi , KM Yang , SD Jeon, JH Kim , LY Khil , TS Chang , Moon CK.
Brazilin modulates immune function mainly by augmenting T cell
activity in halothane administered mice. Planta Med 1997; 63: 405-408.
[18] W Mar , HT Lee , KH Je , HY Choi , EK Seo . A DNA strand-nicking
principle of a higher plant, Caesalpinia sappan. Arch Pharm Res 2003; 26:
147-150
@article{"International Journal of Medical, Medicine and Health Sciences:60650", author = "Liansheng Ren and Xihua Yang and Guoping Wang and Hong Zhang and Lili Zhao and Zhenguo Mi", title = "Inhibition Effect of Brazilin to Human Bladder Cancer Cell Line T24", abstract = "The inhibition effect of brazilin to human bladder
tumor cell line T24 in vitro and in vivo was studied. The results of the
in vitro experiments showed that brazilin has strong inhibition activity
on the target cells. The inhibition ratio of 100 μg/mL brazilin and 100
μg/mL mitomycin to the target cells was 90.90 % and 63.24 %
respectively, which showed that brazilin has higher inhibition activity
than mitomycin under the same concentration. Brazilin could induce
cell apoptosis in T24 cells. Significant antitumor activity of brazilin
was also showed in the animals experiments. The life extention rate of
200 mg/mL, 300 mg/kg, and 400 mg/kg brazilin intraperitoneally
injected into Balb/c-nu-nu nude mice that with human bladder cancer
were 51.50 %, 56.90 %, and 58.42 %（P", keywords = "bladder cancer, brazilin, inhibition, T24 cell line", volume = "5", number = "12", pages = "684-5", }
