Histochemistry of Intestinal Enzymes of Juvenile Dourado Salminus brasiliensis Fed Bovine Colostrum
Enzyme activity was evaluated in the intestine of
juvenile dourado (Salminus brasiliensis) fed with diets containing 0,
10 or 20% of lyophilized bovine colostrum (LBC) inclusion for either
30 or 60 days. The intestinal enzymes acid and alkaline phosphatase
(ACP and ALP, respectively), non-specific esterase (NSE), lipase
(LIP), dipeptidyl aminopeptidase IV (DAP IV) and leucine
aminopeptidase (LAP) were studied using histochemistry in four
intestinal segments (S1, S2, S3 and posterior intestine). Weak
proteolitic activity was observed in all intestinal segments for DAP
IV and LAP. The activity of NSE and LIP was also weak in all
intestines, except for the moderate activity of NSE in the S2 of 20%
LBC group after 30 days and in the S1 of 0% LBC group after 60
days. The ACP was detected only in the S2 and S3 of the 10% LBC
group after 30 days. Moderate and strong staining was observed in
the first three intestinal segments for ALP and weak activity in the
posterior intestine. The activity of DAP IV, LAP and ALP were also
present in the cytoplasm of the enterocytes. In the present results,
bovine colostrum feeding did not cause alterations in activity of
intestinal enzymes.
[1] A. Huguet, B. Sève, J. Le Dividich, and I. Le Huërou-Luron, "Effects of
a bovine colostrum-supplemented diet on some gut parameters in
weaned piglets”, Reproduction Nutrition Development, vol. 46, pp. 167-
178, 2006.
[2] A.L. Lima, P. Pauletti, I. Susin, and R. Machado-Neto, "Fluctuation of
serum variables in goats and comparative study of antibody absorption
in newborn kids using cattle and goat colostrum”, Brazilian Journal of
Animal Science, vol. 38, pp. 2211-2217, 2009.
[3] D.B. Moretti, L. Kindlein, P. Pauletti, and R. Machado-Neto, "IgG
absorption by Santa Ines lambs fed Holstein bovine colostrum or Santa
Ines ovine colostrum”, Animal, vol. 4, pp. 933-937, 2010.
[4] A.P.O Rodrigues, P. Pauletti, L. Kindlein, J.E.P. Cyrino, E.F. Delgado,
and R. Machado-Neto, "Intestinal morphology and histology of the
striped catfish Pseudoplatystoma fasciatum (Linnaeus, 1766) fed dry
diets”, Aquaculture Nutrition, vol. 15, pp. 55-563, 2009.
[5] P. Bodammer, C. Maletzki, G. Waitz, and J. Emmrich, "Prophylatic
application of bovine colostrum ameliorates murine colitis via induction
of immunoregulatory cells”, Journal of Nutrition, vol. 141, pp. 1056-
1061, 2011.
[6] D.B. Moretti, W.M. Nordi, A.L. Lima, P. Pauletti, I. Susin, and R.
Machado-Neto, "Enzyme activity in the small intestine of goat kids
during the period of passive immunity acquisition”, Small Ruminant
Research, vol. 105, pp. 321-328, 2012.
[7] N.N. Pandey, A.A. Dar, D.B. Mondal, and L. Nagaraja, "Bovine
colostrum: A veterinary nutraceutical”, Journal of Veterinary Medicine
and Animal Health, vol. 3, pp. 31-35, 2011.
[8] L.J. Schep, I.G. Tucker, G. Young, R. Ledger, and A.G. Butt,
"Controlled release opportunities for oral peptide delivery in
aquaculture”, Journal of Controlled Release, vol. 59, pp. 1-14, 1999.
[9] R.L. Barbieri, and F.J. Hernández-Blazquez, "Análise ultra-estrutural da
absorção intestinal de macromolécula protéica com o uso de peixe como
modelo experimental”, ConScientiae Saúde, vol. 1, pp. 21-30, 2002.
[10] K. Opuszynski, and J.V. Shireman, "Digestive mechanisms (book
style)”. In Herbivorous Fishes: Culture and Use for Weed Management,
K. Opuszynski and J.V. Shireman, Boca Raton, FL: CRC Press, 1995,
pp.21-31.
[11] R. Ledger, I.G. Tucker, and G.F. Walker, "The metabolic barrier of the
lower intestinal tract of salmon to the oral delivery of protein and
peptide drugs”, Journal of Controlled Release, vol. 85, pp. 91-103, 2002.
[12] D.M. Fracalossi, G. Meyer, F.M. Santamaria, M. Weingastner, and E.
Zaniboni Filho, "Desempenho do jundiá, Rhamdia quelen, e do dourado,
Salminus brasiliensis, em viveiros de terra na Região Sul do Brasil”,
Acta Scientiarum, vol. 26, pp. 345-352, 2004.
[13] Association of Official Analytical Chemists. Official methods of
analysis, 18th ed. vol. 2, Arlington, VA: AOAC, 2004.
[14] S.S. Rodrigues, and E. Menin, "Anatomia do tubo digestivo de Salminus
brasiliensis (Cuvier, 1818) (Pisces, Characidae, Salmininae)”, Biotemas,
vol. 21, pp. 65-75, 2008.
[15] Z. Lodja, "Studies on Dipeptidyl(Amino)Peptidase IV (Glycyl-Proline
Naphthylamidase)”, Histochemistry, vol. 59, pp. 153-166, 1979.
[16] J.D. Bancroft, "Enzyme histochemistry”, in Theory and Practice of
Histological Techniques, vol. 4, J.D. Bancroft, and A. Stevens), New
York, NY: Churchill Livingstone, 1996, pp.391-410.
[17] K.N. Hirji, and W.A.M. Courtney "Leucine aminopeptidase activity in
the digestive tract of perch, Perca fluviatilis L”, Journal of Fish Biology,
vol. 21, pp. 615–622, 1982.
[18] A. Gawlicka, S. Teh, S.S.O. Hung, E. Hinton, and J. De Ll Noüe,
"Histological and histochemical changes in the digestive tract of white
sturgeon larvae during ontogeny”, Fish Physiology and Biochemistry,
vol. 14, pp. 357-371, 1995.
[19] B. Tengjaroenkul, B.J. Smith, T. Caceci, and S.A. Smith, "Distribution
of intestinal enzyme activities along the intestinal tract of cultured Nile
tilapia, Oreochromis niloticus L”, Aquaculture, vol. 182, pp. 317–327,
2000.
[20] V.V. Kuz’mina, "Classical and modern concepts in fish digestion”, in
Feeding and Digestive Functions of Fishes, J.E.P Cyrino, D.B Bureau,
and B.G. Kapoor, Enfield, NH: Science Publishers, 2008, pp. 85-154.
[21] V.V. Kuz’mina, and A.G. Gelman, "Membrane-linked digestion in fish”,
Reviews in Fisheries Science, vol. 5, pp. 99-129, 1997.
[22] E. McLean, B. Rønsholdt, C. Sten, and Najamuddin, "Gastrointestinal
delivery of peptide and protein drugs to aquaculture teleosts”,
Aquaculture, vol. 177, pp. 231-247, 1999.
[23] J. Walford, and T.J. Lam, "Development of digestive tract and
proteolytic enzyme activity in seabass (Lates calcarifer) larvae and
juveniles”, Aquaculture, vol. 109, pp. 187-05, 1993.
[24] J.L. Zambonino Infante, and C.J. Cahu, "High dietary lipid levels
enhance digestive tract maturation and improve Dicentrarchus labrax
larval development”, Journal of Nutrition, vol. 129, pp. 1195-1200,
1999.
[25] V. Buchet, J.L. Zambonino Infante, and C.L. Cahu, "Effect of lipid level
in a compound diet on the development of red drum (Sciaenops
ocellatus) larvae”, Aquaculture, vol. 184, pp. 339-347, 2000.
[26] M.S. Izquierdo, J. Socorro, L. Arantzamendi, and C.M. Hernandez-Cruz,
"Recent advances in lipid nutrition in fish larvae”, Fish Physiology and
Biochemistry, vol. 22, pp. 97-107, 2000.
[27] S. Morais, M. Lacuisse, L.E.C. Conceição, M.T. Dinis, and I.
Rønnestad, "Ontogeny of the digestive capacity of Senegalese sole
(Solea senegalensis), with respect to digestion, absorption and
metabolism of amino acids from Artemia”, Marine Biology, vol. 145,
pp. 243-50, 2004.
[28] B. Savona, C. Tramati, and A. Mazzola, "Digestive enzymes in larvae
and juveniles of farmed sharpsnout seabream (Diplodus puntazzo)
(Cetti, 1777)”, The Open Marine Biology Journal, vol. 5, pp. 47-57,
2011.
[29] Z. Kozarić, Z. Petrinec, S. Kužir, E. Gjurčević, and B. Baždarić,
"Histochemical analyses of digestive enzymes in the intestine of adult
large-scale gurnard (Lepidotrigla cavillone, Lacepède, 1801)”,
Anatomia, Histologia, Embryologia, vol. 40, pp. 314-320, 2011.
[30] K. Baintner, 1994, "Demonstration of acidity intestinal vacuoles of the
suckling rat and pig”, Journal of Histochemistry and Cytochemistry, vol.
42, pp. 231-238, 1994.
[31] I. Hussain, and A. Channa, "Histochemical distribution of lipase and
acid phosphatase in the intestinal tract of the snow trout, Schizothorax
curvifrons Heckel”, Journal of Biological Sciences, vol. 10, pp. 643-647,
2010.
[32] Y. Dupuis, S. Tardivel, Z. Porembska, and P. Fournier, "Effect of some
phosphatase inhibitors on intestinal calcium transfer”, International
Journal of Biochemistry, vol. 23, pp. 175–180, 1991.
[33] A. Mahmood, F. Yamagishi, R. Eliakim, K. DeSchryver-Kecskemeti,
T.L. Gramlich, and D.H. Alpers, "A possible role for rat intestinal
surfactant-like particles in transepithelial triacylglycerol transport”,
Journal of Clinical Investigation, vol. 93, pp. 70–80, 1994.
[34] Z. Kozarić, S. Kužir, Z. Petrinec, E. Gjurčević, and A. Opačak,
"Histochemical distribution of digestive enzymes in intestine of
goldline, Sarpa salpa L. 1758”, Journal of Applied Ichthyology, vol. 22,
pp. 43-48., 2006.
[1] A. Huguet, B. Sève, J. Le Dividich, and I. Le Huërou-Luron, "Effects of
a bovine colostrum-supplemented diet on some gut parameters in
weaned piglets”, Reproduction Nutrition Development, vol. 46, pp. 167-
178, 2006.
[2] A.L. Lima, P. Pauletti, I. Susin, and R. Machado-Neto, "Fluctuation of
serum variables in goats and comparative study of antibody absorption
in newborn kids using cattle and goat colostrum”, Brazilian Journal of
Animal Science, vol. 38, pp. 2211-2217, 2009.
[3] D.B. Moretti, L. Kindlein, P. Pauletti, and R. Machado-Neto, "IgG
absorption by Santa Ines lambs fed Holstein bovine colostrum or Santa
Ines ovine colostrum”, Animal, vol. 4, pp. 933-937, 2010.
[4] A.P.O Rodrigues, P. Pauletti, L. Kindlein, J.E.P. Cyrino, E.F. Delgado,
and R. Machado-Neto, "Intestinal morphology and histology of the
striped catfish Pseudoplatystoma fasciatum (Linnaeus, 1766) fed dry
diets”, Aquaculture Nutrition, vol. 15, pp. 55-563, 2009.
[5] P. Bodammer, C. Maletzki, G. Waitz, and J. Emmrich, "Prophylatic
application of bovine colostrum ameliorates murine colitis via induction
of immunoregulatory cells”, Journal of Nutrition, vol. 141, pp. 1056-
1061, 2011.
[6] D.B. Moretti, W.M. Nordi, A.L. Lima, P. Pauletti, I. Susin, and R.
Machado-Neto, "Enzyme activity in the small intestine of goat kids
during the period of passive immunity acquisition”, Small Ruminant
Research, vol. 105, pp. 321-328, 2012.
[7] N.N. Pandey, A.A. Dar, D.B. Mondal, and L. Nagaraja, "Bovine
colostrum: A veterinary nutraceutical”, Journal of Veterinary Medicine
and Animal Health, vol. 3, pp. 31-35, 2011.
[8] L.J. Schep, I.G. Tucker, G. Young, R. Ledger, and A.G. Butt,
"Controlled release opportunities for oral peptide delivery in
aquaculture”, Journal of Controlled Release, vol. 59, pp. 1-14, 1999.
[9] R.L. Barbieri, and F.J. Hernández-Blazquez, "Análise ultra-estrutural da
absorção intestinal de macromolécula protéica com o uso de peixe como
modelo experimental”, ConScientiae Saúde, vol. 1, pp. 21-30, 2002.
[10] K. Opuszynski, and J.V. Shireman, "Digestive mechanisms (book
style)”. In Herbivorous Fishes: Culture and Use for Weed Management,
K. Opuszynski and J.V. Shireman, Boca Raton, FL: CRC Press, 1995,
pp.21-31.
[11] R. Ledger, I.G. Tucker, and G.F. Walker, "The metabolic barrier of the
lower intestinal tract of salmon to the oral delivery of protein and
peptide drugs”, Journal of Controlled Release, vol. 85, pp. 91-103, 2002.
[12] D.M. Fracalossi, G. Meyer, F.M. Santamaria, M. Weingastner, and E.
Zaniboni Filho, "Desempenho do jundiá, Rhamdia quelen, e do dourado,
Salminus brasiliensis, em viveiros de terra na Região Sul do Brasil”,
Acta Scientiarum, vol. 26, pp. 345-352, 2004.
[13] Association of Official Analytical Chemists. Official methods of
analysis, 18th ed. vol. 2, Arlington, VA: AOAC, 2004.
[14] S.S. Rodrigues, and E. Menin, "Anatomia do tubo digestivo de Salminus
brasiliensis (Cuvier, 1818) (Pisces, Characidae, Salmininae)”, Biotemas,
vol. 21, pp. 65-75, 2008.
[15] Z. Lodja, "Studies on Dipeptidyl(Amino)Peptidase IV (Glycyl-Proline
Naphthylamidase)”, Histochemistry, vol. 59, pp. 153-166, 1979.
[16] J.D. Bancroft, "Enzyme histochemistry”, in Theory and Practice of
Histological Techniques, vol. 4, J.D. Bancroft, and A. Stevens), New
York, NY: Churchill Livingstone, 1996, pp.391-410.
[17] K.N. Hirji, and W.A.M. Courtney "Leucine aminopeptidase activity in
the digestive tract of perch, Perca fluviatilis L”, Journal of Fish Biology,
vol. 21, pp. 615–622, 1982.
[18] A. Gawlicka, S. Teh, S.S.O. Hung, E. Hinton, and J. De Ll Noüe,
"Histological and histochemical changes in the digestive tract of white
sturgeon larvae during ontogeny”, Fish Physiology and Biochemistry,
vol. 14, pp. 357-371, 1995.
[19] B. Tengjaroenkul, B.J. Smith, T. Caceci, and S.A. Smith, "Distribution
of intestinal enzyme activities along the intestinal tract of cultured Nile
tilapia, Oreochromis niloticus L”, Aquaculture, vol. 182, pp. 317–327,
2000.
[20] V.V. Kuz’mina, "Classical and modern concepts in fish digestion”, in
Feeding and Digestive Functions of Fishes, J.E.P Cyrino, D.B Bureau,
and B.G. Kapoor, Enfield, NH: Science Publishers, 2008, pp. 85-154.
[21] V.V. Kuz’mina, and A.G. Gelman, "Membrane-linked digestion in fish”,
Reviews in Fisheries Science, vol. 5, pp. 99-129, 1997.
[22] E. McLean, B. Rønsholdt, C. Sten, and Najamuddin, "Gastrointestinal
delivery of peptide and protein drugs to aquaculture teleosts”,
Aquaculture, vol. 177, pp. 231-247, 1999.
[23] J. Walford, and T.J. Lam, "Development of digestive tract and
proteolytic enzyme activity in seabass (Lates calcarifer) larvae and
juveniles”, Aquaculture, vol. 109, pp. 187-05, 1993.
[24] J.L. Zambonino Infante, and C.J. Cahu, "High dietary lipid levels
enhance digestive tract maturation and improve Dicentrarchus labrax
larval development”, Journal of Nutrition, vol. 129, pp. 1195-1200,
1999.
[25] V. Buchet, J.L. Zambonino Infante, and C.L. Cahu, "Effect of lipid level
in a compound diet on the development of red drum (Sciaenops
ocellatus) larvae”, Aquaculture, vol. 184, pp. 339-347, 2000.
[26] M.S. Izquierdo, J. Socorro, L. Arantzamendi, and C.M. Hernandez-Cruz,
"Recent advances in lipid nutrition in fish larvae”, Fish Physiology and
Biochemistry, vol. 22, pp. 97-107, 2000.
[27] S. Morais, M. Lacuisse, L.E.C. Conceição, M.T. Dinis, and I.
Rønnestad, "Ontogeny of the digestive capacity of Senegalese sole
(Solea senegalensis), with respect to digestion, absorption and
metabolism of amino acids from Artemia”, Marine Biology, vol. 145,
pp. 243-50, 2004.
[28] B. Savona, C. Tramati, and A. Mazzola, "Digestive enzymes in larvae
and juveniles of farmed sharpsnout seabream (Diplodus puntazzo)
(Cetti, 1777)”, The Open Marine Biology Journal, vol. 5, pp. 47-57,
2011.
[29] Z. Kozarić, Z. Petrinec, S. Kužir, E. Gjurčević, and B. Baždarić,
"Histochemical analyses of digestive enzymes in the intestine of adult
large-scale gurnard (Lepidotrigla cavillone, Lacepède, 1801)”,
Anatomia, Histologia, Embryologia, vol. 40, pp. 314-320, 2011.
[30] K. Baintner, 1994, "Demonstration of acidity intestinal vacuoles of the
suckling rat and pig”, Journal of Histochemistry and Cytochemistry, vol.
42, pp. 231-238, 1994.
[31] I. Hussain, and A. Channa, "Histochemical distribution of lipase and
acid phosphatase in the intestinal tract of the snow trout, Schizothorax
curvifrons Heckel”, Journal of Biological Sciences, vol. 10, pp. 643-647,
2010.
[32] Y. Dupuis, S. Tardivel, Z. Porembska, and P. Fournier, "Effect of some
phosphatase inhibitors on intestinal calcium transfer”, International
Journal of Biochemistry, vol. 23, pp. 175–180, 1991.
[33] A. Mahmood, F. Yamagishi, R. Eliakim, K. DeSchryver-Kecskemeti,
T.L. Gramlich, and D.H. Alpers, "A possible role for rat intestinal
surfactant-like particles in transepithelial triacylglycerol transport”,
Journal of Clinical Investigation, vol. 93, pp. 70–80, 1994.
[34] Z. Kozarić, S. Kužir, Z. Petrinec, E. Gjurčević, and A. Opačak,
"Histochemical distribution of digestive enzymes in intestine of
goldline, Sarpa salpa L. 1758”, Journal of Applied Ichthyology, vol. 22,
pp. 43-48., 2006.
@article{"International Journal of Biological, Life and Agricultural Sciences:68051", author = "Debora B. Moretti and Wiolene M. Nordi and Thaline Maira P. Cruz and José Eurico P. Cyrino and Raul Machado-Neto", title = "Histochemistry of Intestinal Enzymes of Juvenile Dourado Salminus brasiliensis Fed Bovine Colostrum", abstract = "Enzyme activity was evaluated in the intestine of
juvenile dourado (Salminus brasiliensis) fed with diets containing 0,
10 or 20% of lyophilized bovine colostrum (LBC) inclusion for either
30 or 60 days. The intestinal enzymes acid and alkaline phosphatase
(ACP and ALP, respectively), non-specific esterase (NSE), lipase
(LIP), dipeptidyl aminopeptidase IV (DAP IV) and leucine
aminopeptidase (LAP) were studied using histochemistry in four
intestinal segments (S1, S2, S3 and posterior intestine). Weak
proteolitic activity was observed in all intestinal segments for DAP
IV and LAP. The activity of NSE and LIP was also weak in all
intestines, except for the moderate activity of NSE in the S2 of 20%
LBC group after 30 days and in the S1 of 0% LBC group after 60
days. The ACP was detected only in the S2 and S3 of the 10% LBC
group after 30 days. Moderate and strong staining was observed in
the first three intestinal segments for ALP and weak activity in the
posterior intestine. The activity of DAP IV, LAP and ALP were also
present in the cytoplasm of the enterocytes. In the present results,
bovine colostrum feeding did not cause alterations in activity of
intestinal enzymes.
", keywords = "Carnivorous fish, enterocyte, intestinal epithelium,
teleost.", volume = "8", number = "9", pages = "1031-5", }
