Improvement of Antibacterial Activity for Ceftazidime by Partially Purified Tannase from Penicillium expansum
Tannase has wide applications in food, beverage, brewing, cosmetics and chemical industries and one of the major applications of tannase is the production of gallic acid. Gallic acid is used for manufacturing of trimethoprim. In the present study, a local fungal strain of Penicillium expansum A4 isolated from spoilt apple samples gave the highest production level of tannase. Tannase was partially purified with a recovery yield of 92.52% and 6.32 fold of purification by precipitation using ammonium sulfate at 50% saturation. Tannase led to increased antimicrobial activity of ceftazidime against Pseudomonas aeruginosa and S. aureus and had a synergism effect at low concentrations of ceftazidime, and thus, tannase may be a useful adjuvant agent for the treatment of many bacterial infections in combination with ceftazidime.
[1] Pepeljnjak, S.; Segvic, M.; Ozegovic, L. (2002). Citrininotoxinogenicity of Penicillium spp. isolated from decaying apples. Braz. J. Microbiol., 33:134-137.
[2] Morales, H.; Marín, S.; Rovira, A.; Ramos, J. and Sanchis, V. (2007). "Patulin accumulation in apples by Penicillium expansum during postharvest stages.LettApplMicrobiol.44 (1): 30–5.
[3] Hamdy, H. S. and Fawzy, E. M. (2012). Economic Production of Tannase by Aspergillus niger van Tiegh Adopting Different Fermentation Protocols and Possible Applications. Romanian Biotechnological Letters, 17(4):7441-7457.
[4] Janisiewicz, W. (2012). Blue Mold. USDA Appilachian fruit research.Retriev.10-22.
[5] Viarous, L.; Handel, N.; Abood, J. K.; Ghoul, M. (2007). The growth and production of patulin mycotoxin by Penicillium expansum on apple fruits and its control by the use of propionic acid and sodium benzoate. Department of Biology, College of Science, University of Setiff. Setiff, Algeria.
[6] Prusky, D.; McEvoy, L. James; Saftner, Robert; Conway, S. William; Jones, Richard (2004). "Relationship Between Host Acidification and Virulence in Penicillium spp. on Apple and Citrus Fruit. Phytopathology.94 (1).
[7] Konstantinou, S.; Karaoglanidis, G. S.; Bardas, G. A.; Minas, I. S.; Doukas, E.; Markoglou, A. N. (2011). "Post-harvest fruit rots of apple in Greece: Pathogen incidence and relationships between fruit quality parameters, cultivar susceptibility and patulin production. Plant Disease 95: 666–672.
[8] Hui, Y. H.(2006). Handbook of Fruits and Fruit Processing. Blackwell. p. 697.
[9] Mohammed, H. S.; Al-Zubaidy, M. M. and Al-Aswad, M. B. (2008). Inhibitory effect of tannic acid extracted from grape seeds and pomegranate peels on some microorganisms. Mesoppotamia J. of Agric., 36(1).
[10] Natarajan, K. and Rajendran, A. (2009). Effect of fermentation parameters on extra cellular tannase production by Lactobacillus plantarum MTCC 1407, E-Journal of Chemistry, 6(4): 979-984.
[11] Mongkholrattanasit, R. and Punrattanasin, N. (2012). Properties of silk fabric dyed with eucalyptus, quercetin, rutin and tannin using padding techniques,” in Proc. RMUTP International Conference: Textiles & Fashion, Bangkok Thailand.
[12] Iqbal, H. and Kapoor, A. A. (2012).Culture conditions for the production of tannase from Trichoderma harzianum MTCC 10841, IJST, 1(11):584-594.
[13] Kumar, R.; Kumar, A.; Nagpal, R.; Sharma, J. and Kumari, A. (2012). A novel and sensitive plate assay for screening of tannase producing bacteria, Ann. Microb., 60:177-179.
[14] Lekha, P. K. and Lonsane, B. K. (1997). Production and application of tannin acyl hydrolase: state of the art, Adv. Appl. Microbiol., 44:215-260.
[15] Aguilar, C. N. and Gutierrez-Sanchez, G. (2001).Review: Sources, properties, applications and potential uses of tannin acyl hydrolase, Food Sci. Tech. Int.,7:373-382.
[16] Domsch, K. H.; Gams , W. and Anderson, T. H. (1980). Compendium of soil fungi, Academic Press , Ltd., London , UK. Vol. 1 , Vol. 2.
[17] Nadaf, N. H. and Ghosh, J. S. (2011). Production, Purification and characterization of Tannase from rhodococcus NCIM 2891. Current Research Journal of Biological Sciences, 3(3):246-253.
[18] Mondal, K. C.; Banerjee, D.; Jana, M. and Pati, B. R. (2001). Colorimetric assay method for determination of tannin acyl hydrolase (EC.3.1.1.20) activity, Analytical Biochemistry, 295:168-171.
[19] Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantitities of protein utilizing the principle of protein dye binding, Annal . Biochem.72:248-254.
[20] National Committee for Clinical Laboratory Standards (NCCLS)(2000). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. NCCLS document, M7-A5.
[21] Rosenberger, D. A.; Engle, C. A. and Meyer, F. W. (2006).Penicillium expansum Invades Apples through Stems during Controlled Atmosphere Storage. Plant Health Progress doi:10.1094/PHP-2006-1213-01-RS.
[22] Xiao, C. L. and Boal, R. J. (2009).Residual Activity of Fludioxonil and Pyrimethanil against Penicillium expansum on Apple Fruit. Plant Disease, 93(10): 1003- 1008.
[23] Muslim, S. N.; Mahammed, A. N.; Musafer, H. K.; AL_Kadmy, Israa M. S.; Shafiq, S. A. and Muslim, S. N.(2015). Detection of the Optimal Conditions for Tannase Productivity and Activity by Erwinia Carotovora. Journal Med. Bioengin.4(3):198-205.
[24] Reddy, B. S. and V. Rathod, V.(2012). Gallic acid production &tannase activity of penicillium purpurogenum employing agro based wastes through solid state fermentation: Influence of pH and temperature, Asian Journal of Biochemical and Pharmaceutical Research, 2(1):59-62, 2012.
[25] Batra, R. K. (2005).Potential tannase producers from the genera Aspergillus and Penicillium. Process Biochemistry, 40(5):1553-1557.
[26] Mukherjee, G. and Banerjee, R. (2006). Effects of temperature, pH and additives on the activity of tannase producted by a coculture Rhizopus oryzae and Aspergillus foetidu. World J.Microbiol.Biotechnol.22: 207-212.
[27] Mahendran, B.; Raman, N. and Kim, D. J. (2006). Purification and characterization of tannase from Paecilomyces variotii: Hydrolysis of tannic acid using immobilized tannase. Applied microbiology and Biotechnology. 70(4): 440-450.
[1] Pepeljnjak, S.; Segvic, M.; Ozegovic, L. (2002). Citrininotoxinogenicity of Penicillium spp. isolated from decaying apples. Braz. J. Microbiol., 33:134-137.
[2] Morales, H.; Marín, S.; Rovira, A.; Ramos, J. and Sanchis, V. (2007). "Patulin accumulation in apples by Penicillium expansum during postharvest stages.LettApplMicrobiol.44 (1): 30–5.
[3] Hamdy, H. S. and Fawzy, E. M. (2012). Economic Production of Tannase by Aspergillus niger van Tiegh Adopting Different Fermentation Protocols and Possible Applications. Romanian Biotechnological Letters, 17(4):7441-7457.
[4] Janisiewicz, W. (2012). Blue Mold. USDA Appilachian fruit research.Retriev.10-22.
[5] Viarous, L.; Handel, N.; Abood, J. K.; Ghoul, M. (2007). The growth and production of patulin mycotoxin by Penicillium expansum on apple fruits and its control by the use of propionic acid and sodium benzoate. Department of Biology, College of Science, University of Setiff. Setiff, Algeria.
[6] Prusky, D.; McEvoy, L. James; Saftner, Robert; Conway, S. William; Jones, Richard (2004). "Relationship Between Host Acidification and Virulence in Penicillium spp. on Apple and Citrus Fruit. Phytopathology.94 (1).
[7] Konstantinou, S.; Karaoglanidis, G. S.; Bardas, G. A.; Minas, I. S.; Doukas, E.; Markoglou, A. N. (2011). "Post-harvest fruit rots of apple in Greece: Pathogen incidence and relationships between fruit quality parameters, cultivar susceptibility and patulin production. Plant Disease 95: 666–672.
[8] Hui, Y. H.(2006). Handbook of Fruits and Fruit Processing. Blackwell. p. 697.
[9] Mohammed, H. S.; Al-Zubaidy, M. M. and Al-Aswad, M. B. (2008). Inhibitory effect of tannic acid extracted from grape seeds and pomegranate peels on some microorganisms. Mesoppotamia J. of Agric., 36(1).
[10] Natarajan, K. and Rajendran, A. (2009). Effect of fermentation parameters on extra cellular tannase production by Lactobacillus plantarum MTCC 1407, E-Journal of Chemistry, 6(4): 979-984.
[11] Mongkholrattanasit, R. and Punrattanasin, N. (2012). Properties of silk fabric dyed with eucalyptus, quercetin, rutin and tannin using padding techniques,” in Proc. RMUTP International Conference: Textiles & Fashion, Bangkok Thailand.
[12] Iqbal, H. and Kapoor, A. A. (2012).Culture conditions for the production of tannase from Trichoderma harzianum MTCC 10841, IJST, 1(11):584-594.
[13] Kumar, R.; Kumar, A.; Nagpal, R.; Sharma, J. and Kumari, A. (2012). A novel and sensitive plate assay for screening of tannase producing bacteria, Ann. Microb., 60:177-179.
[14] Lekha, P. K. and Lonsane, B. K. (1997). Production and application of tannin acyl hydrolase: state of the art, Adv. Appl. Microbiol., 44:215-260.
[15] Aguilar, C. N. and Gutierrez-Sanchez, G. (2001).Review: Sources, properties, applications and potential uses of tannin acyl hydrolase, Food Sci. Tech. Int.,7:373-382.
[16] Domsch, K. H.; Gams , W. and Anderson, T. H. (1980). Compendium of soil fungi, Academic Press , Ltd., London , UK. Vol. 1 , Vol. 2.
[17] Nadaf, N. H. and Ghosh, J. S. (2011). Production, Purification and characterization of Tannase from rhodococcus NCIM 2891. Current Research Journal of Biological Sciences, 3(3):246-253.
[18] Mondal, K. C.; Banerjee, D.; Jana, M. and Pati, B. R. (2001). Colorimetric assay method for determination of tannin acyl hydrolase (EC.3.1.1.20) activity, Analytical Biochemistry, 295:168-171.
[19] Bradford, M. (1976). A rapid and sensitive method for the quantitation of microgram quantitities of protein utilizing the principle of protein dye binding, Annal . Biochem.72:248-254.
[20] National Committee for Clinical Laboratory Standards (NCCLS)(2000). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. NCCLS document, M7-A5.
[21] Rosenberger, D. A.; Engle, C. A. and Meyer, F. W. (2006).Penicillium expansum Invades Apples through Stems during Controlled Atmosphere Storage. Plant Health Progress doi:10.1094/PHP-2006-1213-01-RS.
[22] Xiao, C. L. and Boal, R. J. (2009).Residual Activity of Fludioxonil and Pyrimethanil against Penicillium expansum on Apple Fruit. Plant Disease, 93(10): 1003- 1008.
[23] Muslim, S. N.; Mahammed, A. N.; Musafer, H. K.; AL_Kadmy, Israa M. S.; Shafiq, S. A. and Muslim, S. N.(2015). Detection of the Optimal Conditions for Tannase Productivity and Activity by Erwinia Carotovora. Journal Med. Bioengin.4(3):198-205.
[24] Reddy, B. S. and V. Rathod, V.(2012). Gallic acid production &tannase activity of penicillium purpurogenum employing agro based wastes through solid state fermentation: Influence of pH and temperature, Asian Journal of Biochemical and Pharmaceutical Research, 2(1):59-62, 2012.
[25] Batra, R. K. (2005).Potential tannase producers from the genera Aspergillus and Penicillium. Process Biochemistry, 40(5):1553-1557.
[26] Mukherjee, G. and Banerjee, R. (2006). Effects of temperature, pH and additives on the activity of tannase producted by a coculture Rhizopus oryzae and Aspergillus foetidu. World J.Microbiol.Biotechnol.22: 207-212.
[27] Mahendran, B.; Raman, N. and Kim, D. J. (2006). Purification and characterization of tannase from Paecilomyces variotii: Hydrolysis of tannic acid using immobilized tannase. Applied microbiology and Biotechnology. 70(4): 440-450.
@article{"International Journal of Biological, Life and Agricultural Sciences:76214", author = "Sahira N. Muslim and Alaa N. Mohammed and Saba Saadoon Khazaal and Batool Kadham Salman and Israa M. S. AL-Kadmy and Sraa N. Muslim and Ahmed S. Dwaish and Sawsan Mohammed Kareem and Sarah N. Aziz and Ruaa A. Jasim", title = "Improvement of Antibacterial Activity for Ceftazidime by Partially Purified Tannase from Penicillium expansum", abstract = "Tannase has wide applications in food, beverage, brewing, cosmetics and chemical industries and one of the major applications of tannase is the production of gallic acid. Gallic acid is used for manufacturing of trimethoprim. In the present study, a local fungal strain of Penicillium expansum A4 isolated from spoilt apple samples gave the highest production level of tannase. Tannase was partially purified with a recovery yield of 92.52% and 6.32 fold of purification by precipitation using ammonium sulfate at 50% saturation. Tannase led to increased antimicrobial activity of ceftazidime against Pseudomonas aeruginosa and S. aureus and had a synergism effect at low concentrations of ceftazidime, and thus, tannase may be a useful adjuvant agent for the treatment of many bacterial infections in combination with ceftazidime.
", keywords = "Ceftazidime, Penicillium expansum, tannase, antimicrobial activity.", volume = "11", number = "7", pages = "541-5", }
