Effects of Irradiation to Morphological, Physicochemical and Biocompatibility Properties of Carrageenan
The characterization of κ-carrageenan could provide a
better understanding of its functions in biological, medical and
industrial applications. Chemical and physical analyses of
carrageenan from seaweeds, Euchema cottonii L., were done to offer
information on its properties and the effects of Co-60 γ-irradiation on
its thermochemical characteristics. The structural and morphological
characteristics of κ-carrageenan were determined using scanning
electron microscopy (SEM) while the composition, molecular weight
and thermal properties were determined using attenuated total
reflectance Fourier transform infrared spectroscopy (ATR-FTIR), gel
permeation chromatography (GPC), thermal gravimetric analysis
(TGA) and differential scanning calorimetry (DSC). Further chemical
analysis was done using hydrogen-1 nuclear magnetic resonance (1H
NMR) and functional characteristics in terms of biocompatibility
were evaluated using cytotoxicity test.
[1] Usov, A. I., "Structural analysis of red seaweed galactans of agar and
carrageenan groups," Food Hydrocolloids, vol. 12, pp. 301-308, 1998.
[2] Yermak, I.M., Kim, Y., Titlynov, E., Isakov, V., Solov'eva, T., "Chemical
structure and gel properties of carrageenans from algae belonging to the
Gigartinaceae and Tichocarpaceae, collected from the Russian Pacific
coast," Journal of Applied Phycology, vol. 11, pp. 41-48, 1999.
[3] Michel, A.S., Mestdagh, M.M., and M.A. Axelos, "Physico-chemical
properties of carrageenan gels in presence of various cations,"
International Journal of Biological Molecules, vol. 21, pp. 195-200,
1997.
[4] Knutsen, S., Myslabodski, D., Larsen, B. and A. Usov, "A modified
system of nomenclature for red algal galactans," Botanica Marina, vol.
37, no. 2, pp. 163-169, 1994.
[5] Noseda, M. D. and A. S. Cerezo, "Room temperature, low-field 13CNMR
spectra of degraded carrageenans: Part III. Autohydrolysis of a
lambda carrageenan and of its alkali-treated derivative," International
Journal of Biological Macromolecules, vol. 15, no. 3, pp. 177-181,
1993.
[6] Knutsen, S. H. and H. Grasdalen, "The use of neocarrabiose
oligosaccharides with different length and sulphate substitution as
model compounds for 1H-NMR spectroscopy," Carbohydrate Research,
vol. 229, pp. 233-244, 1992.
[1] Usov, A. I., "Structural analysis of red seaweed galactans of agar and
carrageenan groups," Food Hydrocolloids, vol. 12, pp. 301-308, 1998.
[2] Yermak, I.M., Kim, Y., Titlynov, E., Isakov, V., Solov'eva, T., "Chemical
structure and gel properties of carrageenans from algae belonging to the
Gigartinaceae and Tichocarpaceae, collected from the Russian Pacific
coast," Journal of Applied Phycology, vol. 11, pp. 41-48, 1999.
[3] Michel, A.S., Mestdagh, M.M., and M.A. Axelos, "Physico-chemical
properties of carrageenan gels in presence of various cations,"
International Journal of Biological Molecules, vol. 21, pp. 195-200,
1997.
[4] Knutsen, S., Myslabodski, D., Larsen, B. and A. Usov, "A modified
system of nomenclature for red algal galactans," Botanica Marina, vol.
37, no. 2, pp. 163-169, 1994.
[5] Noseda, M. D. and A. S. Cerezo, "Room temperature, low-field 13CNMR
spectra of degraded carrageenans: Part III. Autohydrolysis of a
lambda carrageenan and of its alkali-treated derivative," International
Journal of Biological Macromolecules, vol. 15, no. 3, pp. 177-181,
1993.
[6] Knutsen, S. H. and H. Grasdalen, "The use of neocarrabiose
oligosaccharides with different length and sulphate substitution as
model compounds for 1H-NMR spectroscopy," Carbohydrate Research,
vol. 229, pp. 233-244, 1992.
@article{"International Journal of Biological, Life and Agricultural Sciences:58834", author = "Jhalique Jane R. Fojas and Rizalinda L. De Leon and Lucille V. Abad", title = "Effects of Irradiation to Morphological, Physicochemical and Biocompatibility Properties of Carrageenan", abstract = "The characterization of κ-carrageenan could provide a
better understanding of its functions in biological, medical and
industrial applications. Chemical and physical analyses of
carrageenan from seaweeds, Euchema cottonii L., were done to offer
information on its properties and the effects of Co-60 γ-irradiation on
its thermochemical characteristics. The structural and morphological
characteristics of κ-carrageenan were determined using scanning
electron microscopy (SEM) while the composition, molecular weight
and thermal properties were determined using attenuated total
reflectance Fourier transform infrared spectroscopy (ATR-FTIR), gel
permeation chromatography (GPC), thermal gravimetric analysis
(TGA) and differential scanning calorimetry (DSC). Further chemical
analysis was done using hydrogen-1 nuclear magnetic resonance (1H
NMR) and functional characteristics in terms of biocompatibility
were evaluated using cytotoxicity test.", keywords = "Biocompatibility, carrageenan, DSC, FTIR, GPC,irradiation, NMR, physicochemical, SEM, TGA.", volume = "7", number = "5", pages = "313-4", }