Study on Phytochemical Properties, Antibacterial Activity and Cytotoxicity of Aloe vera L.
The aim of the study was to investigate phytochemical
properties, antimicrobial activity and cytotoxicity of Aloe vera. The
phytochemical screening of the extracts of leaves of A. vera revealed
the presence of bioactive compounds such as alkaloids, tannins,
flavonoids phenolic compounds, and etc. with absence of cyanogenic
glycosides. Three different solvents such as methanol, ethanol and
Di-Methyl sulfoxide were used to screen the antimicrobial activity of
A. vera leaves against four human clinical pathogens by agar well
diffusion method. The maximum antibacterial activities were
observed in methanol extract followed by ethanol and Di-Methyl
sulfoxide. It was also found that remarkable antibacterial activities
with methanolic and ethanolic extracts of A. vera compared with the
standard antibiotic, tetracycline that was not active against E. coli
and S. boydii and supported the view that A. vera is a potent
antimicrobial agent compared with the conventional antibiotic.
Moreover, the brine shrimps (Artemia salina) toxicity test exhibited
LC50 value was 569.52 ppm. The resulting data indicated that the A.
vera plant have less toxic effects on brine shrimp. Hence, it is
signified that Aloe vera plant extract is safe to be used as an
antimicrobial agent.
[1] H. R. Davis, Aloe vera: A Scientific Approach. Vantage Press, New
York, SA, 1997.
[2] B. C. Coats, The Silent Healer, A modern study of Aloe vera. Texas,
Garland. 1979.
[3] M. Obata, "Mechanisms of anti-inflammatory and anti thermal burn
action of carboxypeptidase from aloe aborescens miller" Natalensis
berger in rats and mice physiotherapy research, vol. 7, Special issues,
pp. 530-533, 1993.
[4] M. S. Shelton, "Aloe vera, its chemical and therapeutic properties,"
International Journal of Dermatology, vol. 30, pp. 679-683, 1991.
[5] P. Green, "Aloe vera extracts in equine clinical practice," Veterinary
Times, vol. 26, pp. 9, 1996.
[6] J. B. Kahlon, "Inhibition of Aids Virus replication by Ale Mannan in
vitro," Molecular Biothermy, vol. 3, pp. 127-135, 1991.
[7] M. A. Sheets, "Studies of the effect of ace Mannon on retrovirus
infections, clinical stabilization of feline leukemia virus infected eats,"
Molecular Biothermy, vol. 3, pp. 41-45, 1991.
[8] L. J. Lorenzetti, R. Salisburry, J. L. Beal and J. N. Baldwin,
"Bacteriostatic property of Aloe vera," J. of the Pharm. Sci., vol.53, pp.
1287-1290, 1964.
[9] P. Sims, M. Ruth and E.R. Zimmerman, "Effect of Aloe vera on Herpes
simplex and herpes virus (Strain Zoster)," Aloe vera of American
Archives, vol. 1, pp. 239-240, 1971.
[10] T. Hirat and T. Suga, "The efficiency of aloe plants, chemical
constituents and biological activities," Cosmetics and toiletries, vol. 98,
pp. 105-108, 1983.
[11] B. C. Coats, The Silent Healer, A modern study of Aloe vera. Texas,
Garland. 1979.
[12] K. H. Lee and J. H. Kim, "Anti-lukaemic and anti -mutagenic effects of
di (2-ethylhexyl) phthalate isolated from Aloe vera Linn.," Journal of
Pharmacy and Pharmacology, vol. 52, no. 5, pp. 593-598, 2000.
[13] K. M. Abdullah, A. Abdullah, M. L. Johnson, J. J. Bilski, K. Petry, D. A.
Redmer, L. P. Reynolds, A. T. Grazul-Bilska, "Effects of Aloe vera
on gap junctional intercellular communication and proliferation of
human diabetic and non-diabetic skin fibroblasts," J. Altern.
Complement. Med., vol. 9, no. 5, pp. 711-718, 2003.
[14] M. S. Shelton, "Aloe vera, its chemical and therapeutic properties,"
International Journal of Dermatology, vol. 30, pp. 679-683, 1991.
[15] K. Fujita, Y. Yamada, K. Azuma and S. Hirozawa, "Effect of leaf
extracts of Aloe arborescens subsp. natalensis on growth of
Trichophyton metagrophytes," Antimicrobial agents and chemotherapy,
vol. 14, pp. 132-136, 1978.
[16] M. L. Dhar, M. M. Dhar, B. N. Dhawab, American Botanical Journal,
pp. 232, 1968.
[17] A. Sofowara, Medicinal plants and Traditional medicine in Africa,
Spectrum Books Ltd., Ibadan, Nigeria, pp. 289, 1993.
[18] J. B. Harborne, Phytochemcial methods, London, Chapman and Hall
Ltd., pp. 49- 188, 1973.
[19] O. O. Agarry, M. T. Olaleye and C. O. Bello-Michael, "Comparative
antimicrobial activities of Aloe vera gel and leaf," African journal of
Biotechnology, vol. 4, no. 12, pp. 1413-1414, Dec. 2005.
[20] B. N. Meyer, N. R. Ferrign, J. E. Putnan, L. B. Jacobsca, D. E. Michols,
and J. L. Molaughlin, Brine Shrimp, A Convenient General Bioassay for
Active Plant Constituents, 1982.
[21] E. Simionatto, C. Porto, U. F. da Silva, A. M. C. Squizani, I. I. Dalcol,
A. F. Morel, J. Braz. Chem. Soc., vol. 16, pp. 1458-1462, 2005.
[1] H. R. Davis, Aloe vera: A Scientific Approach. Vantage Press, New
York, SA, 1997.
[2] B. C. Coats, The Silent Healer, A modern study of Aloe vera. Texas,
Garland. 1979.
[3] M. Obata, "Mechanisms of anti-inflammatory and anti thermal burn
action of carboxypeptidase from aloe aborescens miller" Natalensis
berger in rats and mice physiotherapy research, vol. 7, Special issues,
pp. 530-533, 1993.
[4] M. S. Shelton, "Aloe vera, its chemical and therapeutic properties,"
International Journal of Dermatology, vol. 30, pp. 679-683, 1991.
[5] P. Green, "Aloe vera extracts in equine clinical practice," Veterinary
Times, vol. 26, pp. 9, 1996.
[6] J. B. Kahlon, "Inhibition of Aids Virus replication by Ale Mannan in
vitro," Molecular Biothermy, vol. 3, pp. 127-135, 1991.
[7] M. A. Sheets, "Studies of the effect of ace Mannon on retrovirus
infections, clinical stabilization of feline leukemia virus infected eats,"
Molecular Biothermy, vol. 3, pp. 41-45, 1991.
[8] L. J. Lorenzetti, R. Salisburry, J. L. Beal and J. N. Baldwin,
"Bacteriostatic property of Aloe vera," J. of the Pharm. Sci., vol.53, pp.
1287-1290, 1964.
[9] P. Sims, M. Ruth and E.R. Zimmerman, "Effect of Aloe vera on Herpes
simplex and herpes virus (Strain Zoster)," Aloe vera of American
Archives, vol. 1, pp. 239-240, 1971.
[10] T. Hirat and T. Suga, "The efficiency of aloe plants, chemical
constituents and biological activities," Cosmetics and toiletries, vol. 98,
pp. 105-108, 1983.
[11] B. C. Coats, The Silent Healer, A modern study of Aloe vera. Texas,
Garland. 1979.
[12] K. H. Lee and J. H. Kim, "Anti-lukaemic and anti -mutagenic effects of
di (2-ethylhexyl) phthalate isolated from Aloe vera Linn.," Journal of
Pharmacy and Pharmacology, vol. 52, no. 5, pp. 593-598, 2000.
[13] K. M. Abdullah, A. Abdullah, M. L. Johnson, J. J. Bilski, K. Petry, D. A.
Redmer, L. P. Reynolds, A. T. Grazul-Bilska, "Effects of Aloe vera
on gap junctional intercellular communication and proliferation of
human diabetic and non-diabetic skin fibroblasts," J. Altern.
Complement. Med., vol. 9, no. 5, pp. 711-718, 2003.
[14] M. S. Shelton, "Aloe vera, its chemical and therapeutic properties,"
International Journal of Dermatology, vol. 30, pp. 679-683, 1991.
[15] K. Fujita, Y. Yamada, K. Azuma and S. Hirozawa, "Effect of leaf
extracts of Aloe arborescens subsp. natalensis on growth of
Trichophyton metagrophytes," Antimicrobial agents and chemotherapy,
vol. 14, pp. 132-136, 1978.
[16] M. L. Dhar, M. M. Dhar, B. N. Dhawab, American Botanical Journal,
pp. 232, 1968.
[17] A. Sofowara, Medicinal plants and Traditional medicine in Africa,
Spectrum Books Ltd., Ibadan, Nigeria, pp. 289, 1993.
[18] J. B. Harborne, Phytochemcial methods, London, Chapman and Hall
Ltd., pp. 49- 188, 1973.
[19] O. O. Agarry, M. T. Olaleye and C. O. Bello-Michael, "Comparative
antimicrobial activities of Aloe vera gel and leaf," African journal of
Biotechnology, vol. 4, no. 12, pp. 1413-1414, Dec. 2005.
[20] B. N. Meyer, N. R. Ferrign, J. E. Putnan, L. B. Jacobsca, D. E. Michols,
and J. L. Molaughlin, Brine Shrimp, A Convenient General Bioassay for
Active Plant Constituents, 1982.
[21] E. Simionatto, C. Porto, U. F. da Silva, A. M. C. Squizani, I. I. Dalcol,
A. F. Morel, J. Braz. Chem. Soc., vol. 16, pp. 1458-1462, 2005.
@article{"International Journal of Biological, Life and Agricultural Sciences:54307", author = "K. Thu and Yin Y. Mon and Tin A. Khaing and Ohn M. Tun", title = "Study on Phytochemical Properties, Antibacterial Activity and Cytotoxicity of Aloe vera L.", abstract = "The aim of the study was to investigate phytochemical
properties, antimicrobial activity and cytotoxicity of Aloe vera. The
phytochemical screening of the extracts of leaves of A. vera revealed
the presence of bioactive compounds such as alkaloids, tannins,
flavonoids phenolic compounds, and etc. with absence of cyanogenic
glycosides. Three different solvents such as methanol, ethanol and
Di-Methyl sulfoxide were used to screen the antimicrobial activity of
A. vera leaves against four human clinical pathogens by agar well
diffusion method. The maximum antibacterial activities were
observed in methanol extract followed by ethanol and Di-Methyl
sulfoxide. It was also found that remarkable antibacterial activities
with methanolic and ethanolic extracts of A. vera compared with the
standard antibiotic, tetracycline that was not active against E. coli
and S. boydii and supported the view that A. vera is a potent
antimicrobial agent compared with the conventional antibiotic.
Moreover, the brine shrimps (Artemia salina) toxicity test exhibited
LC50 value was 569.52 ppm. The resulting data indicated that the A.
vera plant have less toxic effects on brine shrimp. Hence, it is
signified that Aloe vera plant extract is safe to be used as an
antimicrobial agent.", keywords = "Aloe vera L., antimicrobial activity, brine shrimp,cytotoxicity, phytochemical properties.", volume = "7", number = "5", pages = "304-5", }