The Effectiveness of Ultrasound Treatment on the Germination Stimulation of Barley Seed and its Alpha-Amylase Activity
In the present study, the effects of ultrasound as
emerging technology were investigated on germination stimulation,
amount of alpha-amylase activity on dry barley seeds before steeping
stage of malting process. All experiments were carried out at 20 KHz
on the ultrasonic generator in 3 different ultrasonic intensities (20, 60
and 100% setting from total power of device) and time (5, 10 and 15
min) at constant temperature (30C). For determining the effects of
these parameters on enzyme the Fuwa method assay based on the
decreased staining value of blue starch–iodine complexes employed
for measurement an activity. The results of these assays were
analyzed by Qualitek4 software using the Taguchi statistical method
to evaluate the factor-s effects on enzyme activity. It has been found
that when malting barley is irradiated with an ultrasonic power, a
stimulating effect occurs as to the enzyme activity.
[1] G. Muralikrishna, M. Nirmala, "Cereal alpha-amylases-an overview",
Carbo. Poly. 60, 163-173, 2005.
[2] D.Tull, B. A. Phillipson, B. Kramh├╣ft, S. Knudsen, O. Olsen and B.
Svensson, " Enhanced amylolytic activity in germinating barley through
synthesis of a bacterial alpha-amylase" , Journal of Cereal Science,37,
71-80,2003.
[3] L.Taiz and J. E. Starks, "Gibberellic Acid enhancement of DNA turnover
in barley aleurone cells", Plant Physiol, 60,182-189, 1977.
[4] K. C. Eastwell and M. S. Spencer, "Modes of ethylene action in the
release of amylase from barley aleurone layers", Plant Physiol, 67, 563-
567, 1982.
[5] M. J. W. Povey and T. J. Mason, "Ultrasound in food processing",
International Thomson Publishing I T P pp 115-125, 1998.
[6] A.G, Gordon, The use of ultrasound in agriculture.Ultrason.1(2),pp 70-77,
1963.
[7] M. Toma, M. Vinatoru, L. Paniwnyk , T.J. Mason, "investigation of the
effects of ultrasound on vegetal tissues during solvent extraction",
ultrason. sonoch.8,137-142,2001.
[8] J.D Shors, D. R. Soll, K. J. Daniels and D.P.Gibson,method for enhancing
germination, United state application publication,patent
No.5,950,362,1999.
[9] S. Shimomura, The effects of ultrasonic irradiation on sprouting radish
seed, Ultrasonics Symposium, Proceedings., IEEE , page(s) vol.3, 1665-
1667, 1990.
[9] A. Aladjadjiyan, ,Increasing carrot seeds (Daucus carota L.), cv. Nantes,
viability through ultrasound treatment. Bulg. J. Agric. Sci., 8, 469-472,
2002.
[10] S.A. Hebling and W.R. da. Silva, Effects of low intensity ultrasound on
the germination of corn seeds (Zea mays L.) under different water
availabilities. Sci. agric. (Piracicaba, Braz.),52(3) p.514-520,1995.
[11] P. Weinberger and M. Measures, The effect of two audible sound
frequencies on the germination and growth of a spring and winter wheat,
Can. J. Bot. 46(9), 1151-1158 ,1968.
[12] A.M. Osman, " The advantages of using natural substrate-based methods
in assessing the roles and synergistic and competitive intractions of
barley malt starch-degrading enzymes", J. Inst. Brew. 108(2), 204-214,
2002.
[13] R. Gupta, P. Gigras, H. Mohapatra, G.V. Kumar, B. Chauhan, "Microbial
a-amylases: a biotechnological perspective", Process Biochem. 38, 1599-
/1616, 2003.
[14] Z. Xiao, R. Storms, A. Tsang, "A quantitative starch-iodine method for
measuring alpha-amylase and glucoamylase activities", Analytical
Biochemistry 351,146-148, 2006.
[15] Suslick K.S.. Sonochemistry. Science, 247, 1439-1445, 1990.
[1] G. Muralikrishna, M. Nirmala, "Cereal alpha-amylases-an overview",
Carbo. Poly. 60, 163-173, 2005.
[2] D.Tull, B. A. Phillipson, B. Kramh├╣ft, S. Knudsen, O. Olsen and B.
Svensson, " Enhanced amylolytic activity in germinating barley through
synthesis of a bacterial alpha-amylase" , Journal of Cereal Science,37,
71-80,2003.
[3] L.Taiz and J. E. Starks, "Gibberellic Acid enhancement of DNA turnover
in barley aleurone cells", Plant Physiol, 60,182-189, 1977.
[4] K. C. Eastwell and M. S. Spencer, "Modes of ethylene action in the
release of amylase from barley aleurone layers", Plant Physiol, 67, 563-
567, 1982.
[5] M. J. W. Povey and T. J. Mason, "Ultrasound in food processing",
International Thomson Publishing I T P pp 115-125, 1998.
[6] A.G, Gordon, The use of ultrasound in agriculture.Ultrason.1(2),pp 70-77,
1963.
[7] M. Toma, M. Vinatoru, L. Paniwnyk , T.J. Mason, "investigation of the
effects of ultrasound on vegetal tissues during solvent extraction",
ultrason. sonoch.8,137-142,2001.
[8] J.D Shors, D. R. Soll, K. J. Daniels and D.P.Gibson,method for enhancing
germination, United state application publication,patent
No.5,950,362,1999.
[9] S. Shimomura, The effects of ultrasonic irradiation on sprouting radish
seed, Ultrasonics Symposium, Proceedings., IEEE , page(s) vol.3, 1665-
1667, 1990.
[9] A. Aladjadjiyan, ,Increasing carrot seeds (Daucus carota L.), cv. Nantes,
viability through ultrasound treatment. Bulg. J. Agric. Sci., 8, 469-472,
2002.
[10] S.A. Hebling and W.R. da. Silva, Effects of low intensity ultrasound on
the germination of corn seeds (Zea mays L.) under different water
availabilities. Sci. agric. (Piracicaba, Braz.),52(3) p.514-520,1995.
[11] P. Weinberger and M. Measures, The effect of two audible sound
frequencies on the germination and growth of a spring and winter wheat,
Can. J. Bot. 46(9), 1151-1158 ,1968.
[12] A.M. Osman, " The advantages of using natural substrate-based methods
in assessing the roles and synergistic and competitive intractions of
barley malt starch-degrading enzymes", J. Inst. Brew. 108(2), 204-214,
2002.
[13] R. Gupta, P. Gigras, H. Mohapatra, G.V. Kumar, B. Chauhan, "Microbial
a-amylases: a biotechnological perspective", Process Biochem. 38, 1599-
/1616, 2003.
[14] Z. Xiao, R. Storms, A. Tsang, "A quantitative starch-iodine method for
measuring alpha-amylase and glucoamylase activities", Analytical
Biochemistry 351,146-148, 2006.
[15] Suslick K.S.. Sonochemistry. Science, 247, 1439-1445, 1990.
@article{"International Journal of Biological, Life and Agricultural Sciences:50179", author = "M. Yaldagard and S.A. Mortazavi and F. Tabatabaie", title = "The Effectiveness of Ultrasound Treatment on the Germination Stimulation of Barley Seed and its Alpha-Amylase Activity", abstract = "In the present study, the effects of ultrasound as
emerging technology were investigated on germination stimulation,
amount of alpha-amylase activity on dry barley seeds before steeping
stage of malting process. All experiments were carried out at 20 KHz
on the ultrasonic generator in 3 different ultrasonic intensities (20, 60
and 100% setting from total power of device) and time (5, 10 and 15
min) at constant temperature (30C). For determining the effects of
these parameters on enzyme the Fuwa method assay based on the
decreased staining value of blue starch–iodine complexes employed
for measurement an activity. The results of these assays were
analyzed by Qualitek4 software using the Taguchi statistical method
to evaluate the factor-s effects on enzyme activity. It has been found
that when malting barley is irradiated with an ultrasonic power, a
stimulating effect occurs as to the enzyme activity.", keywords = "ultrasound, alpha-amylase activity, stimulationand Taguchi statistical method.", volume = "1", number = "10", pages = "115-6", }