The Effect of Electrical Stimulation Intensity on VEGF Expression and Biomechanical Properties during Wound
We evaluated the effect of sensory (direct current
(DC), 600μA) and motor (monophasic current, pulse duration 300μs,
100 Hz, 2.5-3mA) intensities of cathodal electrical stimulation (ES)
current to release VEGF and biomechanical properties of wound. 54
male Sprague-dawley rats were randomly assigned into one control
and two experimental groups. A full thickness skin incision was
made on animals- dorsal region. The experimental groups received
ES for 1h/day and every other day. VEGF expression was measured
in skin on the 7th day after surgical incision and tensile strength was
measured on 21st day. On the 7th day, the values of skin VEGF in the
sensory group were significantly greater than those of the other
groups (p < 0.05). Sensory and Motor intensity stimulation, can not
improve the biomechanical properties of the repaired wounds.
It seems the mechanical environment induced by sensory and
motor intensity of electrical stimulation, could not simulate the role
of normal daily stress and strain to maturation of collagen fibers and
their cross links. Further work is needed to determine the relationship
between VEGF expression after ES and its effect on tensile strength
of healed wound.
[1] P. Bao , A.Kodra , M.Tomic-Canic , M.S. Golinko, H.P. Ehrlich, H.
Brem," The role of vascular endothelial growth factor in wound
healing", J Surge Res. vol. 153, no. 2, pp. 347-358, 2009.
[2] K.A. Morris, M.F. McGee, J.J. Jasper, K.M. Bogie. "Evaluation of
electrical stimulation for ischemic wound therapy: a feasibility study
using the lapine wound model", Arch Dermatol Res. Vol. 301, no. 4, pp.
323-327, 2009.
[3] M. Zhao, H. Bai, E. Wang, J.V. Forrester, C.D. McCaige, " CD.
Electrically stimulation directly induces pre-angiogenic responses in
vascular endothelial cells by signaling through VEGF receptors", J cell
Sci. no. 117, pp. 397-405, 2004.
[4] G. Talebi, G. Torkaman, M. Firoozabadi, S. Shariat, " Effect of anodal
and cathodal micro-amperage direct current on the skin wound healing:
A biomechanical and histological study", Journal of biomechanics, no.
40(s2), p. s665, 2007.
[5] L.C. Kloth, J.M. McCulloch, "Promotion of wound healing with
electrical stimulation", Adv Wound care. Vol. 9, no. 5, pp. 42-45,
1996.G. O. Young, "Synthetic structure of industrial plastics (Book style
with paper title and editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed.
New York: McGraw-Hill, 1964, pp. 15-64.
[1] P. Bao , A.Kodra , M.Tomic-Canic , M.S. Golinko, H.P. Ehrlich, H.
Brem," The role of vascular endothelial growth factor in wound
healing", J Surge Res. vol. 153, no. 2, pp. 347-358, 2009.
[2] K.A. Morris, M.F. McGee, J.J. Jasper, K.M. Bogie. "Evaluation of
electrical stimulation for ischemic wound therapy: a feasibility study
using the lapine wound model", Arch Dermatol Res. Vol. 301, no. 4, pp.
323-327, 2009.
[3] M. Zhao, H. Bai, E. Wang, J.V. Forrester, C.D. McCaige, " CD.
Electrically stimulation directly induces pre-angiogenic responses in
vascular endothelial cells by signaling through VEGF receptors", J cell
Sci. no. 117, pp. 397-405, 2004.
[4] G. Talebi, G. Torkaman, M. Firoozabadi, S. Shariat, " Effect of anodal
and cathodal micro-amperage direct current on the skin wound healing:
A biomechanical and histological study", Journal of biomechanics, no.
40(s2), p. s665, 2007.
[5] L.C. Kloth, J.M. McCulloch, "Promotion of wound healing with
electrical stimulation", Adv Wound care. Vol. 9, no. 5, pp. 42-45,
1996.G. O. Young, "Synthetic structure of industrial plastics (Book style
with paper title and editor)," in Plastics, 2nd ed. vol. 3, J. Peters, Ed.
New York: McGraw-Hill, 1964, pp. 15-64.
@article{"International Journal of Medical, Medicine and Health Sciences:56628", author = "M R Asadi and G Torkaman and M Hedayati", title = "The Effect of Electrical Stimulation Intensity on VEGF Expression and Biomechanical Properties during Wound", abstract = "We evaluated the effect of sensory (direct current
(DC), 600μA) and motor (monophasic current, pulse duration 300μs,
100 Hz, 2.5-3mA) intensities of cathodal electrical stimulation (ES)
current to release VEGF and biomechanical properties of wound. 54
male Sprague-dawley rats were randomly assigned into one control
and two experimental groups. A full thickness skin incision was
made on animals- dorsal region. The experimental groups received
ES for 1h/day and every other day. VEGF expression was measured
in skin on the 7th day after surgical incision and tensile strength was
measured on 21st day. On the 7th day, the values of skin VEGF in the
sensory group were significantly greater than those of the other
groups (p < 0.05). Sensory and Motor intensity stimulation, can not
improve the biomechanical properties of the repaired wounds.
It seems the mechanical environment induced by sensory and
motor intensity of electrical stimulation, could not simulate the role
of normal daily stress and strain to maturation of collagen fibers and
their cross links. Further work is needed to determine the relationship
between VEGF expression after ES and its effect on tensile strength
of healed wound.", keywords = "Biomechanical properties Direct current,Monophasic current, Skin, VEGF", volume = "4", number = "9", pages = "438-3", }