The Cytotoxic Effect of PM 701 and its Fractions on Cell Proliferation of Breast Cancer Cells, McF7
Breast cancer is the most common malignancy in the
world among women. Many therapies have been designed to treat
this disease. Mamectomy, chemotherapy and radiotherapy are still
the main therapies of breast cancer. However, the results were
unsatisfactory and still far from the ideal treatment.
PM 701is a natural product, has anticancer activity. The bioactive
fraction PMF and subfraction PMFK had been isolated from PM701.
PM 701 and its fractions were proved to have a cytotoxic properties
against different cancer cell lines. This article is directed for the
further examination of lyophilized PM701 and its active fractions on
the growth of breast cancer cells (MCF-7). PM 701, PMF or PMFK
were adding to the cultural medium, where MCF-7 is incubated.
PM 701, PMF or PMFK were able to inhibit significantly the
proliferation of MCF-7 cells, Moreover these new agents were
proved to induce apoptosis of the breast cancer cells; through its
direct effect on the nuclei.
[1] Coufal, M., M.M. Maxwell, Russel, D.E. Amore, A.M. S.M. Altmann,
Z.R. Hollingsworth, A.B. Young, D.E. Housman, and A.G.
Kazantsev, 2007. Discovery of a novel small-molecule targeting
selective clearance of mutant huntingtin fragments. J Biomol Screen,
12(3):351-60.
[2] He, X., and R.H. Liu, 2007. Triterpenoids Isolated from Apple Peels
Have Potent Antiproliferative Activity and May Be Partially
Responsible for Apple's Anticancer Activity. J. Agric. Food Chem.,
55(11):4366-70.
[3] Widodo N., K. Kaur, B.G. Shrestha, Y. Takagi, T. Ishii, Wadhwa R. and
S.C. Kaul, 2007. Selective killing of cancer cells by leaf extract of
Ashwagandha: identification of a tumor-inhibitory factor and the first
molecular insights to its effect. Clin Cancer Res., 13(7):2298-306.
[4] Feng, L., L.F. Zhang , T.J. Yan, J. Jin, and W.Y. Tao, 2006. Studies on
active substance of anticancer effect in Polygonum cuspidatum. Zhong
Yao Cai., 29(7):689-691.
[5] Khorshid F.A., Shazly H., Al-Jefery A., and Osman A.A. Dose
Escalation Phase I Study in Healthy Volunteers to Evaluate the Safety of
a natural product PM 701. Int. J. of pharmacology and toxicology,5(3):
91-9, 2010.
[6] Grever, M. and B.Chabner, . Cancer drug discovery and development in
cancer propels and practice of oncology. Cytotoxicity of some medicinal
plant extracts used in Tanzanian traditional medicine.
J.Ethnopharmacol., 1997 ,70:143-149.
[7] Moshref SS : PM701 A Highly Selective Anti Cancerous Agent Against
L1210 Leukemic Cells: II - In Vivo Clinical And Histopathological
Study. JKAU- Medical Sciences ,2007,Vol 14 (1), pp.85-99.
[8] Schwartsmann, G, Ratain, MJ, Cragg, GM, Wong, JE, Saijo, N,
Parkinson, DR, Fujiwara, Y, Pazdur, R, Newman, DJ, Dagher, R, Di
Leone, L :Anticancer drug discovery and development throughout the
world. J. Clin. Oncol. 20 , 2002, (suppl):47S-59S.
[9] Khorshid, F.A., Mosherf, S.S. & Tawfiq, N.H. : An ideal selective
anticancer agent in vitro, I- Tissue culture study of human lung cancer
cells A549. JKAU-Medical Sciences, 12,3-19, 2005.
[10] Khorshid, F.A., : Preclinical evaluation of PM 701 in Experimental
animals. International Journal of Pharmacology, 2008 ,4(6): 443-451,
ISSN 1811-7775.
[11] El-Shahawy A, El-Sawi N., Backer W.S., Wadiah S. Backer, Khorshid
F.A., and Geweely N.S. Spectral Analysis, Molecular Orbital
Calculations And Antimicrobial Activity Of PMF-G Fraction Extracted
From PM-701. Int. J. of Pharma and Bioscience, vol. 1(2): p 1-19, 2010.
[12] Khorshid FA, Moshref SS, Jamal Y. The Effect of PM 701 on Mice
Leukemic Cells:I - Tissue Culture Study of L1210 (In Vitro) II - In Vivo
Study on Mice, JKAU- Medical Sciences .Vol 13 (1), pp. 3-19, 2006.
[13] Khorshid FA. Osman AA. Abdulsattar E. Cytotoxicity of Bioactive
fractions from PM 701. EJEAFChe, 8 (11), 2009. [1091-1098].
[14] Ptak, A., Ludewig, G., Pak, A., Nadolna, W., Bochenek, M. &
Gregoraszczuk, E.L. : Induction of cytochrome P450 1A1 in MCF-7
human breast cancer cells by 4-chlorobiphenyl (PCB3) and the effects of
its hydroxylated metabolites on cellular apoptosis. Environment
International (2009).
[15] Pollard JW, Walker JM.:Methods in Molecular Biology. vol. 5. Animal
Cell Culture.Clifton, NJ: Human P, 1989, 2-10.
[16] Khorshid FA. Comparative Study of Keloid Formation in Animal
Models, Med Sci Monit, 11(7): BR 212-219; 2005.
[17] Khorshid FA. Moshref SS. In Vitro Anticancer Agent, I - Tissue Culture
Study of Human Lung Cancer Cells A549 II - Tissue Culture Study of
Mice Leukemia Cells L1210. International Journal of Cancer Research 2
(4):330-344, 2006.
[18] Devarajan, E, Chen, J, Multani, AS et al.: Human breast cancer MCF-7
cell line contains inherently drug-resistance subclones with distinct
genotypic and phenotypic features. Int. J. Oncol., 2002 , 20:913-920.
[19] Raouf GA, Khorshid FA; Kumosani T. :FT-IR Spectroscopy as a Tool
for Identification of Apoptosis-Induced Structural Changes in A549
Cells Dry Samples Treated with PM 701. Int. J. Nano and Biomaterials,
2009 , Vol. 2, No. 1/2/3/4/5.
[20] Masuda, M.; Suzui, M. and Weinstein, I.B. : Effects of
.epigallocatechin-3-gallate on growth, epidermal growth factor.receptor
signaling pathways, gene expression, an .chemosensitivity in.human
head and neck squamous cell.carcinoma cell lines. Clin Cancer Res.,
2001,7:4220-9.
[21] Masuda,M.;Suzui,M.;Lim,J.T.and.Weinstein,I.B.: Epigallocatechin- 3-
gallate inhibits.activation of HER-2/neu and downstream signaling
.pathways in human head and neck and breast carcinoma cells. Clin
Cancer Res., 2003, 9:3486-91.
[22] Huh,S.W.; Bae, S.M.; Kim, Y.W. et al. :Anticancer effects of (-)-
.epigallocatechin-3- gallate on ovarian carcinoma cell lines. Gynecol.
Oncol., 2004, 94 (3): 760-768.
[23] Chan, M.M.; Soprano, K.J.; Weinstein,K. and Fong,D,:
Epigallocatechin-3-gallate delivers hydrogen peroxide to induce death.of
ovarian cancer cells and enhances their cisplatin susceptibility. J Cell
Physiol., 2006, 207: 389-96.
[24] Parkinson, A. : Biotransformation of xenobiotics. Klassen, C. D. eds.
Cassarett and Doull-s Toxicology: The Basic Science of Poisons 5th ed.
1996:113-186 McGraw-Hill New York.
[1] Coufal, M., M.M. Maxwell, Russel, D.E. Amore, A.M. S.M. Altmann,
Z.R. Hollingsworth, A.B. Young, D.E. Housman, and A.G.
Kazantsev, 2007. Discovery of a novel small-molecule targeting
selective clearance of mutant huntingtin fragments. J Biomol Screen,
12(3):351-60.
[2] He, X., and R.H. Liu, 2007. Triterpenoids Isolated from Apple Peels
Have Potent Antiproliferative Activity and May Be Partially
Responsible for Apple's Anticancer Activity. J. Agric. Food Chem.,
55(11):4366-70.
[3] Widodo N., K. Kaur, B.G. Shrestha, Y. Takagi, T. Ishii, Wadhwa R. and
S.C. Kaul, 2007. Selective killing of cancer cells by leaf extract of
Ashwagandha: identification of a tumor-inhibitory factor and the first
molecular insights to its effect. Clin Cancer Res., 13(7):2298-306.
[4] Feng, L., L.F. Zhang , T.J. Yan, J. Jin, and W.Y. Tao, 2006. Studies on
active substance of anticancer effect in Polygonum cuspidatum. Zhong
Yao Cai., 29(7):689-691.
[5] Khorshid F.A., Shazly H., Al-Jefery A., and Osman A.A. Dose
Escalation Phase I Study in Healthy Volunteers to Evaluate the Safety of
a natural product PM 701. Int. J. of pharmacology and toxicology,5(3):
91-9, 2010.
[6] Grever, M. and B.Chabner, . Cancer drug discovery and development in
cancer propels and practice of oncology. Cytotoxicity of some medicinal
plant extracts used in Tanzanian traditional medicine.
J.Ethnopharmacol., 1997 ,70:143-149.
[7] Moshref SS : PM701 A Highly Selective Anti Cancerous Agent Against
L1210 Leukemic Cells: II - In Vivo Clinical And Histopathological
Study. JKAU- Medical Sciences ,2007,Vol 14 (1), pp.85-99.
[8] Schwartsmann, G, Ratain, MJ, Cragg, GM, Wong, JE, Saijo, N,
Parkinson, DR, Fujiwara, Y, Pazdur, R, Newman, DJ, Dagher, R, Di
Leone, L :Anticancer drug discovery and development throughout the
world. J. Clin. Oncol. 20 , 2002, (suppl):47S-59S.
[9] Khorshid, F.A., Mosherf, S.S. & Tawfiq, N.H. : An ideal selective
anticancer agent in vitro, I- Tissue culture study of human lung cancer
cells A549. JKAU-Medical Sciences, 12,3-19, 2005.
[10] Khorshid, F.A., : Preclinical evaluation of PM 701 in Experimental
animals. International Journal of Pharmacology, 2008 ,4(6): 443-451,
ISSN 1811-7775.
[11] El-Shahawy A, El-Sawi N., Backer W.S., Wadiah S. Backer, Khorshid
F.A., and Geweely N.S. Spectral Analysis, Molecular Orbital
Calculations And Antimicrobial Activity Of PMF-G Fraction Extracted
From PM-701. Int. J. of Pharma and Bioscience, vol. 1(2): p 1-19, 2010.
[12] Khorshid FA, Moshref SS, Jamal Y. The Effect of PM 701 on Mice
Leukemic Cells:I - Tissue Culture Study of L1210 (In Vitro) II - In Vivo
Study on Mice, JKAU- Medical Sciences .Vol 13 (1), pp. 3-19, 2006.
[13] Khorshid FA. Osman AA. Abdulsattar E. Cytotoxicity of Bioactive
fractions from PM 701. EJEAFChe, 8 (11), 2009. [1091-1098].
[14] Ptak, A., Ludewig, G., Pak, A., Nadolna, W., Bochenek, M. &
Gregoraszczuk, E.L. : Induction of cytochrome P450 1A1 in MCF-7
human breast cancer cells by 4-chlorobiphenyl (PCB3) and the effects of
its hydroxylated metabolites on cellular apoptosis. Environment
International (2009).
[15] Pollard JW, Walker JM.:Methods in Molecular Biology. vol. 5. Animal
Cell Culture.Clifton, NJ: Human P, 1989, 2-10.
[16] Khorshid FA. Comparative Study of Keloid Formation in Animal
Models, Med Sci Monit, 11(7): BR 212-219; 2005.
[17] Khorshid FA. Moshref SS. In Vitro Anticancer Agent, I - Tissue Culture
Study of Human Lung Cancer Cells A549 II - Tissue Culture Study of
Mice Leukemia Cells L1210. International Journal of Cancer Research 2
(4):330-344, 2006.
[18] Devarajan, E, Chen, J, Multani, AS et al.: Human breast cancer MCF-7
cell line contains inherently drug-resistance subclones with distinct
genotypic and phenotypic features. Int. J. Oncol., 2002 , 20:913-920.
[19] Raouf GA, Khorshid FA; Kumosani T. :FT-IR Spectroscopy as a Tool
for Identification of Apoptosis-Induced Structural Changes in A549
Cells Dry Samples Treated with PM 701. Int. J. Nano and Biomaterials,
2009 , Vol. 2, No. 1/2/3/4/5.
[20] Masuda, M.; Suzui, M. and Weinstein, I.B. : Effects of
.epigallocatechin-3-gallate on growth, epidermal growth factor.receptor
signaling pathways, gene expression, an .chemosensitivity in.human
head and neck squamous cell.carcinoma cell lines. Clin Cancer Res.,
2001,7:4220-9.
[21] Masuda,M.;Suzui,M.;Lim,J.T.and.Weinstein,I.B.: Epigallocatechin- 3-
gallate inhibits.activation of HER-2/neu and downstream signaling
.pathways in human head and neck and breast carcinoma cells. Clin
Cancer Res., 2003, 9:3486-91.
[22] Huh,S.W.; Bae, S.M.; Kim, Y.W. et al. :Anticancer effects of (-)-
.epigallocatechin-3- gallate on ovarian carcinoma cell lines. Gynecol.
Oncol., 2004, 94 (3): 760-768.
[23] Chan, M.M.; Soprano, K.J.; Weinstein,K. and Fong,D,:
Epigallocatechin-3-gallate delivers hydrogen peroxide to induce death.of
ovarian cancer cells and enhances their cisplatin susceptibility. J Cell
Physiol., 2006, 207: 389-96.
[24] Parkinson, A. : Biotransformation of xenobiotics. Klassen, C. D. eds.
Cassarett and Doull-s Toxicology: The Basic Science of Poisons 5th ed.
1996:113-186 McGraw-Hill New York.
@article{"International Journal of Medical, Medicine and Health Sciences:57117", author = "Faten A. Khorshid", title = "The Cytotoxic Effect of PM 701 and its Fractions on Cell Proliferation of Breast Cancer Cells, McF7", abstract = "Breast cancer is the most common malignancy in the
world among women. Many therapies have been designed to treat
this disease. Mamectomy, chemotherapy and radiotherapy are still
the main therapies of breast cancer. However, the results were
unsatisfactory and still far from the ideal treatment.
PM 701is a natural product, has anticancer activity. The bioactive
fraction PMF and subfraction PMFK had been isolated from PM701.
PM 701 and its fractions were proved to have a cytotoxic properties
against different cancer cell lines. This article is directed for the
further examination of lyophilized PM701 and its active fractions on
the growth of breast cancer cells (MCF-7). PM 701, PMF or PMFK
were adding to the cultural medium, where MCF-7 is incubated.
PM 701, PMF or PMFK were able to inhibit significantly the
proliferation of MCF-7 cells, Moreover these new agents were
proved to induce apoptosis of the breast cancer cells; through its
direct effect on the nuclei.", keywords = "Anticancer agent, breast carcinoma, MCF-7 cell
line, PM 701", volume = "5", number = "1", pages = "23-5", }
