Study on Nitrite Accumulation Characteristics and Nitrifying Population Dynamics at Different Growth Environments
Novel nitrogen removal technologies via nitrite
pathway attract increasing interest in recent years. In this study,
batch experiments were performed to investigate nitrite accumulation
characteristics and shifts in nitrifying community structure at
different growth environments including ammonia concentration, pH
and alkalinity. It was found that nitrite accumulation ratios were
maintained at around 95% at studied conditions, and the optimum pH
and Alk/N (ratio between alkalinity and nitrogen) for ammonium
oxidization were 8.5 and 8.33, respectively. Fluorescence in situ
hybridization analysis of nitrifying bacteria showed that high free
ammonia (from influent ammonium or caused by high pH)
significantly altered the structure of nitrifying community, leading to
abundance of ammonia-oxidizing bacteria (AOB), especially
Nitrososmonas, and inhibition of nitrite-oxidizing bacteria (NOB).
The results suggest that free ammonia plays more important role than
other studied conditions on nitrite accumulation.
[1] Michael R, Aoife L, Xiao LW (2006) Carbon and nitrogen removal
using a novel horizontal flow biofilm system. Process Biochemistry 41:
2270-2275.
[2] Verstraete W, Philips S (1998) Nitrification-denitrification processes
and technologies in new contexts. Environ Pollut 102: 717-726.
[3] Park DH, Daniel R, Noguera (2004) Evaluating the effect of dissolved
oxygen on ammonia-oxidizing bacteria communities in activated sludge.
Water Research 38: 3275-3286.
[4] Kim DG, Kim SH (2006) Effect of nitrite concentration on the
distribution and competition of nitrite-oxidizing bacteria in nitratation
reactor systems and their kinetic characteristics. Water Research 40:
887-894.
[5] Abeling U, Seyfried CF (1992) Anaerobic-aerobic treatment of highstrength
ammonia wastewater nitrogen removal via nitrite. Water Sci
Technol 26: 1007-1015
[6] Ciudad G, lez R Gonza', Bornhardt C, Antileo C (2007) Modes of
operation and pH control as enhancement factors for partial nitrification
with oxygen transport limitation. Water Research 636: 1-9.
[7] Kim DJ, Chang JS, Lee DI, Han DW, Yoo IK, Cha GC (2003)
Nitrification of high strength ammonia wastewater and nitrite
accumulation characteristics. Water Sci Technol 4711: 45-51.
[8] Fdz-Polanco F, Villaverde S, Garcia PA (1996) Nitrite accumulation in
submerged biofilters-combined effects. Water Sci Technol 343: 371-
378.
[9] Amann RI (1995) In situ identification of micro-organisms by whole cell
hybridization with rRNA-targeted nucleic acid probes. Kluwer
Academic Publishers.
[10] Koops HP, Pommerening-Roser A (2001) Distribution and
ecophysiology of the nitrifying bacteria emphasizing cultured species.
FEMS Microbiology Ecology 37: 1-9.
[11] APHA (1998) Stand methods for the examination of waster and
wastewater. American Public Health Association, Washington, DC.
[12] Amann RI, Binder BJ, Olson RJ (1990) Combination of 16S rDNAtargeted
ologomucleotide probes with flow cytometry for analyzing
mixed microbial populations. Appl Environ Microbiol 56: 1919-1925.
[13] Mobarry BK, Wagner M, Urbain V, Rittmann BE, Stahl DA (1996)
Phylogenetic probes for analyzing abundance and spatial organization of
nitrifying bacteria. Appl Environ Biotechnol 62: 2156-2162.
[14] Wagner M, Rath G, Koops HP, Flood J, Amann R (1996) In situ analysis
of nitrifying bacteria in sewage treatment plants. Water Sci Technol 34:
237-244.
[15] Daims H, Nielsen P, Nielsen JL (2000) Novel nitrospira-like bacteria as
dominant nitrite-oxidizers in biofilm from wastewater treatment plants:
diversity and in situ physiology. Water Sci Technol 41: 85-90.
[16] Jih CG, Huang JS, Lin HJ, Chou HH (2008) Comparative kinetic
behavior of nitrifiers with different growth environments. Bioresource
Technology 99: 3484-3490.
[17] Stehr G, Bottcher B, Dittberner P, Rath G, Koops HP (1995) The
ammonia-oxidizing nitrifying population of the river Elbe estuary.
FEMS Microbiology Ecology 17: 177-186.
[18] Turk O, Mavinic DS (1989) Maintaining nitrite build-up in a system
acclimated to free ammonia. Water Research 23: 1383-1388.
[19] Park SJ, Bae W, Chung JW, Baek SC (2007) Empirical model of the pH
dependence of the maximum specific nitrification rate. Process
Biochemistry 42: 1671-1676.
[20] Anthonisen AC, Loehr RC, Prakasam TBS, Srimath EG (1976)
Inhibition of nitrification of ammonia and nitrous acid. J Water Pollution
Control Fed 485: 835-852.
[1] Michael R, Aoife L, Xiao LW (2006) Carbon and nitrogen removal
using a novel horizontal flow biofilm system. Process Biochemistry 41:
2270-2275.
[2] Verstraete W, Philips S (1998) Nitrification-denitrification processes
and technologies in new contexts. Environ Pollut 102: 717-726.
[3] Park DH, Daniel R, Noguera (2004) Evaluating the effect of dissolved
oxygen on ammonia-oxidizing bacteria communities in activated sludge.
Water Research 38: 3275-3286.
[4] Kim DG, Kim SH (2006) Effect of nitrite concentration on the
distribution and competition of nitrite-oxidizing bacteria in nitratation
reactor systems and their kinetic characteristics. Water Research 40:
887-894.
[5] Abeling U, Seyfried CF (1992) Anaerobic-aerobic treatment of highstrength
ammonia wastewater nitrogen removal via nitrite. Water Sci
Technol 26: 1007-1015
[6] Ciudad G, lez R Gonza', Bornhardt C, Antileo C (2007) Modes of
operation and pH control as enhancement factors for partial nitrification
with oxygen transport limitation. Water Research 636: 1-9.
[7] Kim DJ, Chang JS, Lee DI, Han DW, Yoo IK, Cha GC (2003)
Nitrification of high strength ammonia wastewater and nitrite
accumulation characteristics. Water Sci Technol 4711: 45-51.
[8] Fdz-Polanco F, Villaverde S, Garcia PA (1996) Nitrite accumulation in
submerged biofilters-combined effects. Water Sci Technol 343: 371-
378.
[9] Amann RI (1995) In situ identification of micro-organisms by whole cell
hybridization with rRNA-targeted nucleic acid probes. Kluwer
Academic Publishers.
[10] Koops HP, Pommerening-Roser A (2001) Distribution and
ecophysiology of the nitrifying bacteria emphasizing cultured species.
FEMS Microbiology Ecology 37: 1-9.
[11] APHA (1998) Stand methods for the examination of waster and
wastewater. American Public Health Association, Washington, DC.
[12] Amann RI, Binder BJ, Olson RJ (1990) Combination of 16S rDNAtargeted
ologomucleotide probes with flow cytometry for analyzing
mixed microbial populations. Appl Environ Microbiol 56: 1919-1925.
[13] Mobarry BK, Wagner M, Urbain V, Rittmann BE, Stahl DA (1996)
Phylogenetic probes for analyzing abundance and spatial organization of
nitrifying bacteria. Appl Environ Biotechnol 62: 2156-2162.
[14] Wagner M, Rath G, Koops HP, Flood J, Amann R (1996) In situ analysis
of nitrifying bacteria in sewage treatment plants. Water Sci Technol 34:
237-244.
[15] Daims H, Nielsen P, Nielsen JL (2000) Novel nitrospira-like bacteria as
dominant nitrite-oxidizers in biofilm from wastewater treatment plants:
diversity and in situ physiology. Water Sci Technol 41: 85-90.
[16] Jih CG, Huang JS, Lin HJ, Chou HH (2008) Comparative kinetic
behavior of nitrifiers with different growth environments. Bioresource
Technology 99: 3484-3490.
[17] Stehr G, Bottcher B, Dittberner P, Rath G, Koops HP (1995) The
ammonia-oxidizing nitrifying population of the river Elbe estuary.
FEMS Microbiology Ecology 17: 177-186.
[18] Turk O, Mavinic DS (1989) Maintaining nitrite build-up in a system
acclimated to free ammonia. Water Research 23: 1383-1388.
[19] Park SJ, Bae W, Chung JW, Baek SC (2007) Empirical model of the pH
dependence of the maximum specific nitrification rate. Process
Biochemistry 42: 1671-1676.
[20] Anthonisen AC, Loehr RC, Prakasam TBS, Srimath EG (1976)
Inhibition of nitrification of ammonia and nitrous acid. J Water Pollution
Control Fed 485: 835-852.
@article{"International Journal of Earth, Energy and Environmental Sciences:64546", author = "Yunxia Zhang and Jiti Zhou and Jianbo Guo and Xiuhong Zhang and Lihong Zhao and Shouzhi Yuan", title = "Study on Nitrite Accumulation Characteristics and Nitrifying Population Dynamics at Different Growth Environments", abstract = "Novel nitrogen removal technologies via nitrite
pathway attract increasing interest in recent years. In this study,
batch experiments were performed to investigate nitrite accumulation
characteristics and shifts in nitrifying community structure at
different growth environments including ammonia concentration, pH
and alkalinity. It was found that nitrite accumulation ratios were
maintained at around 95% at studied conditions, and the optimum pH
and Alk/N (ratio between alkalinity and nitrogen) for ammonium
oxidization were 8.5 and 8.33, respectively. Fluorescence in situ
hybridization analysis of nitrifying bacteria showed that high free
ammonia (from influent ammonium or caused by high pH)
significantly altered the structure of nitrifying community, leading to
abundance of ammonia-oxidizing bacteria (AOB), especially
Nitrososmonas, and inhibition of nitrite-oxidizing bacteria (NOB).
The results suggest that free ammonia plays more important role than
other studied conditions on nitrite accumulation.", keywords = "Partial nitrification, Nitrite accumulation, Nitrifyingbacteria, Fluorescence in situ hybridization (FISH).", volume = "5", number = "4", pages = "278-5", }
