Somatic Embryogenesis for Agropyron cristatum on Murashige and Skoog Medium
Agropyron cristatum L. Gaertn. is a native grass of
semiarid region in Iran which is quit resistant to cool and drought
climate and withstand heavy grazing. This species has close
phylogenetic relationship with Triticum and Hordeum. In this
research, the effect of seven different concentrations of growth
regulator 2,4-D on callus production and somatic embryogenesis of
A. cristatum was investigated on Murashige and Skoog medium. The
results showed that the rate of callus, embryo and neomorph were
highest in 1 mg L-1 2,4-D. Callus production was increased in 1 mg
L-1 2,4-D but dramatically decreased at 5.5 and 9 mg L-1 2,4-D. The
somatic embryos were observed at 1 and 4 mg L-1 2,4-D but matured
embryos and plantlet were only occurred at 1 mg L-1 2,4-D. There
were significant differences between 1 mg L-1 2,4-D and other
treatments for producing globular and torpedo embryos, plantlet,
rooted callus and number of roots (p<0.05) and there was not any
callus production and embryogenesis in control treatment without
growth regulator.
[1] Arunyanart, S., Chaitrayagun, M. (2005). Induction of somatic
embryogenesis in lotus (Nelumbo nucifera Geartn.). Scientia Hortic,
105: 411-420.
[2] S., Smith, R.H. (1990). Regeneration in cereal tissue culture: a review.
Crop Sci.30:1328-1336.
[3] Bor, N.L. (1970). Gramineae, Tribus VII. Triticeae Dumort. P. 147-244.
In K.H. Rechinger(ed.) Flora Iranica, Vol. 70 Akademische Druck-u.
Verlagsanstalt, Graz, Austria.
[4] Dillman, A.C. 1946. The beginnings of crested wheatgrass in North
America. J of the American Society of Agronomy, 38(3): 237-250.
[5] Grando, F.M., Franklin, C.I., Shatters Jr R.G. (2002). Optimizing
embryogenic callus production and plant regeneration from ÔÇÿTifton 9-
Bahia grass seed explants for genetic manipulation. Plant Cell Tissue
Organ Cult, 71:213-222.
[6] Gupta, S., Khanna, V.K., Singh, R., Garg, C.K. (2006). Strategies for
overcoming genotypic limitations of in vitro regeneration and
determination of genetic components of variability of plant regeneration
traits in sorghum. Plant Cell Tissue Organ Cult, 86:379- 388.
[7] Li, R., Bruneau, A. H., R. Qu. (2006). Improved plant regeneration and
in vitro somatic embryogenesis of St Augustine grass (Stenotaphrum
secundatum (Walt.) Kunze). Plant Breed. 125:52-56.
[8] Previati, A., Benelli, C., Da Re, F., Ozudogru, A., Lambardi, M. (2008).
Micropropagation and in vitro conservation of virus-free rose
germplasm. Prop Ornam Plants, 8(2): 93-98.
[9] Skirvin, R.M., Norton, M., McPheteers, K.D. (1993). Somaclonal
variation: has it proved useful for plant improvement? Acta Hortic. 336:
333-340.
[10] Vasil, I.K. (1983). Regeneration of plants from single cells of cereals
grasses. In: Lurquin, P.F., Kleinhofs, A. (Eds.), Genetic Engineering in
Eukaryotes. Plenum, NY, pp. 233-252.
[1] Arunyanart, S., Chaitrayagun, M. (2005). Induction of somatic
embryogenesis in lotus (Nelumbo nucifera Geartn.). Scientia Hortic,
105: 411-420.
[2] S., Smith, R.H. (1990). Regeneration in cereal tissue culture: a review.
Crop Sci.30:1328-1336.
[3] Bor, N.L. (1970). Gramineae, Tribus VII. Triticeae Dumort. P. 147-244.
In K.H. Rechinger(ed.) Flora Iranica, Vol. 70 Akademische Druck-u.
Verlagsanstalt, Graz, Austria.
[4] Dillman, A.C. 1946. The beginnings of crested wheatgrass in North
America. J of the American Society of Agronomy, 38(3): 237-250.
[5] Grando, F.M., Franklin, C.I., Shatters Jr R.G. (2002). Optimizing
embryogenic callus production and plant regeneration from ÔÇÿTifton 9-
Bahia grass seed explants for genetic manipulation. Plant Cell Tissue
Organ Cult, 71:213-222.
[6] Gupta, S., Khanna, V.K., Singh, R., Garg, C.K. (2006). Strategies for
overcoming genotypic limitations of in vitro regeneration and
determination of genetic components of variability of plant regeneration
traits in sorghum. Plant Cell Tissue Organ Cult, 86:379- 388.
[7] Li, R., Bruneau, A. H., R. Qu. (2006). Improved plant regeneration and
in vitro somatic embryogenesis of St Augustine grass (Stenotaphrum
secundatum (Walt.) Kunze). Plant Breed. 125:52-56.
[8] Previati, A., Benelli, C., Da Re, F., Ozudogru, A., Lambardi, M. (2008).
Micropropagation and in vitro conservation of virus-free rose
germplasm. Prop Ornam Plants, 8(2): 93-98.
[9] Skirvin, R.M., Norton, M., McPheteers, K.D. (1993). Somaclonal
variation: has it proved useful for plant improvement? Acta Hortic. 336:
333-340.
[10] Vasil, I.K. (1983). Regeneration of plants from single cells of cereals
grasses. In: Lurquin, P.F., Kleinhofs, A. (Eds.), Genetic Engineering in
Eukaryotes. Plenum, NY, pp. 233-252.
@article{"International Journal of Biological, Life and Agricultural Sciences:63799", author = "Masoume Amirkhani and Kambiz Mashayekhi and Maurizio Lambardi", title = "Somatic Embryogenesis for Agropyron cristatum on Murashige and Skoog Medium", abstract = "Agropyron cristatum L. Gaertn. is a native grass of
semiarid region in Iran which is quit resistant to cool and drought
climate and withstand heavy grazing. This species has close
phylogenetic relationship with Triticum and Hordeum. In this
research, the effect of seven different concentrations of growth
regulator 2,4-D on callus production and somatic embryogenesis of
A. cristatum was investigated on Murashige and Skoog medium. The
results showed that the rate of callus, embryo and neomorph were
highest in 1 mg L-1 2,4-D. Callus production was increased in 1 mg
L-1 2,4-D but dramatically decreased at 5.5 and 9 mg L-1 2,4-D. The
somatic embryos were observed at 1 and 4 mg L-1 2,4-D but matured
embryos and plantlet were only occurred at 1 mg L-1 2,4-D. There
were significant differences between 1 mg L-1 2,4-D and other
treatments for producing globular and torpedo embryos, plantlet,
rooted callus and number of roots (p", keywords = "2,4-D, callus production, somatic embryogenesis,Agropyron cristatum.", volume = "4", number = "8", pages = "684-3", }