Screening of Process Variables for the Production of Extracellular Lipase from Palm Oil by Trichoderma Viride using Plackett-Burman Design
Plackett-Burman statistical screening of media
constituents and operational conditions for extracellular lipase
production from isolate Trichoderma viride has been carried out in
submerged fermentation. This statistical design is used in the early
stages of experimentation to screen out unimportant factors from a
large number of possible factors. This design involves screening of
up to 'n-1' variables in just 'n' number of experiments. Regression
coefficients and t-values were calculated by subjecting the
experimental data to statistical analysis using Minitab version 15.
The effects of nine process variables were studied in twelve
experimental trials. Maximum lipase activity of 7.83 μmol /ml /min
was obtained in the 6th trail. Pareto chart illustrates the order of
significance of the variables affecting the lipase production. The
present study concludes that the most significant variables affecting
lipase production were found to be palm oil, yeast extract, K2HPO4,
MgSO4 and CaCl2.
[1] F.Burkert, F. Maugeri, and M.I. Rodrigues, "Optimization of
extracellular lipase production by Geotrichum sp. using factorial
design." Biores. Technol., vol.91, pp. 77-84, 2004.
[2] M. Chaturvedi, M. Singh, M.R. Chugh, and S. Pandey, "Lipase
production from Bacillus subtilis MTCC 6808 by solid state
fermentation using ground nut oil cake as substrate," Res. J. Microbiol.,
vol.5, pp. 725-730, 2010.
[3] K. Ohnish, Y. Yoshida, and J. Sekiguchi, "Lipase production of
Aspergillus oryzae," J. Ferment. Bioeng., vol. 77, pp.490-495, 1994.
[4] H. Abbas, A. Hiol, V. Deyris, and L. Comeau, "Isolation and
characterization of an extracellular lipase from Mucor sp. strain isolated
from palm fruit," Enzyme Microbial Technol., vol 31, pp. 968-975,
2002.
[5] A. Hiol, M.D. Jonzo, N. Rugani, D. Druet, L. Sarda, and L.C. Comeau,
"Purification and characterization of an extracellular lipase from a
thermophilic Rhizopus oryzae strain isolated from palm fruit," Enz.
Microb. Technol., vol. 26, pp.421-430, 2000.
[6] E.S. Kamimura, O. Medieta, M.I. Rodrigues, and F. Maugeri, "Studies
on lipase affinity adsorption using response-surface analysis,"
Biotechnol. Appl. Biochem., vol.33, pp.153-159, 2001.
[7] R.Gulati, R.K. Saxena, R. Gupta, R.P. Yadav, and W. Sheba Davidson,
"Parametric optimisation of Aspergillus terreus lipase production and its
potential in ester synthesis," Process Biochem., vol.35, pp. 459-464,
1999.
[8] M.A.Kashmiri, A. Adnan, and B.W. Butt, "Production, purification and
partial characterization of lipase from Trichoderma viride," Afr. J.
Biotechnol., vol. 5, pp. 878-882, 2006.
[9] E.M. Rajesh, R. Arthe, R. Rajendran, C. Balakumar, N. Pradeepa and S.
Anitha, "Investigation of lipase production by Trichoderma reesei and
optimization of production parameters," EJEAF Che., vol. 9, pp. 1177-
1189,2010.
[10] M.M.D. Maia, A. Heasley, M.M. Camargo de Morais, E.H.M. Melo,
M.A. Jr. Morais, W.M. Ledingham, and J.L. Lima Filho, "Effect of
culture conditions on lipase production by Fusarium solani in batch
fermentation," Biores. Technol., vol. 76, pp. 23-27, 2001.
[11] J.A. Rodriguez, T. Bhagnagar, S. Roussos, J. Cordova, and J. Baratti,
"Improving lipase production by nutrient source modification using
Rhizopus homthallicus cultured in solid state fermentation," Process
Biochem., vol.41, pp. 2264-2269,2006.
[12] D.V. Desmukh, and P.R. Puranik, 2010, "Application of Plackett-
Burman design to evaluate media components affecting antibacterial
activity of alkaliphilic cyanobacteria isolated from Lonar Lake," Turk. J.
Biochem., vol. 35, no. 2, pp. 114-120, 2010.
[13] G.M. Maciel, L.P.S. Vandenberghe, C.W.I. Haminiuk, and C.R. Soccol.
"Xylanase production by Aspergillus niger LPB 326 in solid-State
fermentation using statistical experimental designs. Xylanase production
by A. niger in SSF," Food Technol. Biotechnol., vol. 46, pp. 183-189,
2008.
[14] S.B. Imandi, S.K. Karanam, and H.R. Garapati, "Optimization of media
constituents for the production of lipase in solid state fermentation by
Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)," Adv.
Biosci. Biotechnol., vol.1, pp. 115-121, 2010.
[15] D.A. Akano, and O.O. Atanda, "The present level of aflatoxin in
Nigerian groundnut cake (kulikuli)," Lett. Applied Microbiol., vol. 10,
pp.187-189.1990.
[16] M.G. Valeria, K. Lima-Nadia, M. Maria-Inez, A. Sarquis-David, P.
Mitchell-Luiz, and D. Ramos-Jose, "Production of Lipase by
Penicillium aurantiogriseum," Food Technol. Biotechnol., vol. 41, pp.
105-110, 2003.
[17] S. Fad─▒loglu, and Z. Soylemez, "Kinetics of lipase-catalyzed hydrolysis
of olive oil," Food Res. Intern., vol. 30, pp. 171-175,1997.
[18] F. Pedreschi, K. Kaack, and K. Granby, "The effect of asparaginase on
acrylamide formation in French fries," Food Chem., vol.109,pp.386-
392,2008.
[19] N.A. Soliman, M.B. Mahmoud, and Y.R. Abdel-Fattah, "Polyglutamic
acid production by Bacillus sp. SAB-26: Application of Plackett-
Burman experimental design to evaluate culture requirements," Appl.
Microbiol. Biotechnol., vol. 69, pp. 259-267,2005.
[20] K.W. Domsch, W. Gams and T.H. Anderson, "Compendium of Soil
Fungi," Academic Press, London, pp: 859. 1980.
[21] G. Dhanya, S. Swetha, N.K. Madhavan, S. Rajeev and P. Ashok,
"Response surface methodology for the optimization of alpha amylase
production by Bacillus amyloliquefaciens,- Biores. Technol., vol. 99, pp.
4597-4602,2008.
[22] H. Treichel, D. de Oliveira, M. A. Mazutti, M. D. Luccio, and J.
Vladimir Oliveira, "A review on microbial lipases production food,"
Bioprocess Technol., vol.3, pp.182-196,2010.
[23] N. Nadia, Z.A. Nehad, A.E. Elsayed, M.A. Essam, and M.A. Hanan,
"Optimization of lipase synthesis by Mucor racemosus production in a
triple impeller bioreactor," Malaysian J. Microbiol., vol. 6, pp. 7-
15,2010.
[24] D. Maccio, A. Fabra, and S. Castro, "Acidity and calcium interaction
affect the growth of Bradyrhizobium sp. and attachment to peanut
roots," Soil Biol. Biochem., vol. 34, pp.201-208, 2002.
[25] G. Baskar, and S. Renganathan, "Statistical screening of process
variables for the production of L-asparaginase from cornflour by
Aspergillus terreus MTCC 1782 in submerged fermentation," Indian J.
Sci. Technol., vol. 2, pp. 45-48, 2009.
[26] M. Hymavathi, T. Sathish, P. Brahmaiah, and R. S. Prakasham, "Impact
of carbon and nitrogen sources on L-asparaginase production by isolated
Bacillus circulans (MTCC 8574): Application of saturated Plackett-
Burman design," Chem. Biochem. Eng. Q., vol. 24, no. 4, pp. 473-480,
2010.
[1] F.Burkert, F. Maugeri, and M.I. Rodrigues, "Optimization of
extracellular lipase production by Geotrichum sp. using factorial
design." Biores. Technol., vol.91, pp. 77-84, 2004.
[2] M. Chaturvedi, M. Singh, M.R. Chugh, and S. Pandey, "Lipase
production from Bacillus subtilis MTCC 6808 by solid state
fermentation using ground nut oil cake as substrate," Res. J. Microbiol.,
vol.5, pp. 725-730, 2010.
[3] K. Ohnish, Y. Yoshida, and J. Sekiguchi, "Lipase production of
Aspergillus oryzae," J. Ferment. Bioeng., vol. 77, pp.490-495, 1994.
[4] H. Abbas, A. Hiol, V. Deyris, and L. Comeau, "Isolation and
characterization of an extracellular lipase from Mucor sp. strain isolated
from palm fruit," Enzyme Microbial Technol., vol 31, pp. 968-975,
2002.
[5] A. Hiol, M.D. Jonzo, N. Rugani, D. Druet, L. Sarda, and L.C. Comeau,
"Purification and characterization of an extracellular lipase from a
thermophilic Rhizopus oryzae strain isolated from palm fruit," Enz.
Microb. Technol., vol. 26, pp.421-430, 2000.
[6] E.S. Kamimura, O. Medieta, M.I. Rodrigues, and F. Maugeri, "Studies
on lipase affinity adsorption using response-surface analysis,"
Biotechnol. Appl. Biochem., vol.33, pp.153-159, 2001.
[7] R.Gulati, R.K. Saxena, R. Gupta, R.P. Yadav, and W. Sheba Davidson,
"Parametric optimisation of Aspergillus terreus lipase production and its
potential in ester synthesis," Process Biochem., vol.35, pp. 459-464,
1999.
[8] M.A.Kashmiri, A. Adnan, and B.W. Butt, "Production, purification and
partial characterization of lipase from Trichoderma viride," Afr. J.
Biotechnol., vol. 5, pp. 878-882, 2006.
[9] E.M. Rajesh, R. Arthe, R. Rajendran, C. Balakumar, N. Pradeepa and S.
Anitha, "Investigation of lipase production by Trichoderma reesei and
optimization of production parameters," EJEAF Che., vol. 9, pp. 1177-
1189,2010.
[10] M.M.D. Maia, A. Heasley, M.M. Camargo de Morais, E.H.M. Melo,
M.A. Jr. Morais, W.M. Ledingham, and J.L. Lima Filho, "Effect of
culture conditions on lipase production by Fusarium solani in batch
fermentation," Biores. Technol., vol. 76, pp. 23-27, 2001.
[11] J.A. Rodriguez, T. Bhagnagar, S. Roussos, J. Cordova, and J. Baratti,
"Improving lipase production by nutrient source modification using
Rhizopus homthallicus cultured in solid state fermentation," Process
Biochem., vol.41, pp. 2264-2269,2006.
[12] D.V. Desmukh, and P.R. Puranik, 2010, "Application of Plackett-
Burman design to evaluate media components affecting antibacterial
activity of alkaliphilic cyanobacteria isolated from Lonar Lake," Turk. J.
Biochem., vol. 35, no. 2, pp. 114-120, 2010.
[13] G.M. Maciel, L.P.S. Vandenberghe, C.W.I. Haminiuk, and C.R. Soccol.
"Xylanase production by Aspergillus niger LPB 326 in solid-State
fermentation using statistical experimental designs. Xylanase production
by A. niger in SSF," Food Technol. Biotechnol., vol. 46, pp. 183-189,
2008.
[14] S.B. Imandi, S.K. Karanam, and H.R. Garapati, "Optimization of media
constituents for the production of lipase in solid state fermentation by
Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)," Adv.
Biosci. Biotechnol., vol.1, pp. 115-121, 2010.
[15] D.A. Akano, and O.O. Atanda, "The present level of aflatoxin in
Nigerian groundnut cake (kulikuli)," Lett. Applied Microbiol., vol. 10,
pp.187-189.1990.
[16] M.G. Valeria, K. Lima-Nadia, M. Maria-Inez, A. Sarquis-David, P.
Mitchell-Luiz, and D. Ramos-Jose, "Production of Lipase by
Penicillium aurantiogriseum," Food Technol. Biotechnol., vol. 41, pp.
105-110, 2003.
[17] S. Fad─▒loglu, and Z. Soylemez, "Kinetics of lipase-catalyzed hydrolysis
of olive oil," Food Res. Intern., vol. 30, pp. 171-175,1997.
[18] F. Pedreschi, K. Kaack, and K. Granby, "The effect of asparaginase on
acrylamide formation in French fries," Food Chem., vol.109,pp.386-
392,2008.
[19] N.A. Soliman, M.B. Mahmoud, and Y.R. Abdel-Fattah, "Polyglutamic
acid production by Bacillus sp. SAB-26: Application of Plackett-
Burman experimental design to evaluate culture requirements," Appl.
Microbiol. Biotechnol., vol. 69, pp. 259-267,2005.
[20] K.W. Domsch, W. Gams and T.H. Anderson, "Compendium of Soil
Fungi," Academic Press, London, pp: 859. 1980.
[21] G. Dhanya, S. Swetha, N.K. Madhavan, S. Rajeev and P. Ashok,
"Response surface methodology for the optimization of alpha amylase
production by Bacillus amyloliquefaciens,- Biores. Technol., vol. 99, pp.
4597-4602,2008.
[22] H. Treichel, D. de Oliveira, M. A. Mazutti, M. D. Luccio, and J.
Vladimir Oliveira, "A review on microbial lipases production food,"
Bioprocess Technol., vol.3, pp.182-196,2010.
[23] N. Nadia, Z.A. Nehad, A.E. Elsayed, M.A. Essam, and M.A. Hanan,
"Optimization of lipase synthesis by Mucor racemosus production in a
triple impeller bioreactor," Malaysian J. Microbiol., vol. 6, pp. 7-
15,2010.
[24] D. Maccio, A. Fabra, and S. Castro, "Acidity and calcium interaction
affect the growth of Bradyrhizobium sp. and attachment to peanut
roots," Soil Biol. Biochem., vol. 34, pp.201-208, 2002.
[25] G. Baskar, and S. Renganathan, "Statistical screening of process
variables for the production of L-asparaginase from cornflour by
Aspergillus terreus MTCC 1782 in submerged fermentation," Indian J.
Sci. Technol., vol. 2, pp. 45-48, 2009.
[26] M. Hymavathi, T. Sathish, P. Brahmaiah, and R. S. Prakasham, "Impact
of carbon and nitrogen sources on L-asparaginase production by isolated
Bacillus circulans (MTCC 8574): Application of saturated Plackett-
Burman design," Chem. Biochem. Eng. Q., vol. 24, no. 4, pp. 473-480,
2010.
@article{"International Journal of Biological, Life and Agricultural Sciences:50423", author = "R. Rajendiran and S. Gayathri devi and B.T. SureshKumar and V. Arul Priya", title = "Screening of Process Variables for the Production of Extracellular Lipase from Palm Oil by Trichoderma Viride using Plackett-Burman Design", abstract = "Plackett-Burman statistical screening of media
constituents and operational conditions for extracellular lipase
production from isolate Trichoderma viride has been carried out in
submerged fermentation. This statistical design is used in the early
stages of experimentation to screen out unimportant factors from a
large number of possible factors. This design involves screening of
up to 'n-1' variables in just 'n' number of experiments. Regression
coefficients and t-values were calculated by subjecting the
experimental data to statistical analysis using Minitab version 15.
The effects of nine process variables were studied in twelve
experimental trials. Maximum lipase activity of 7.83 μmol /ml /min
was obtained in the 6th trail. Pareto chart illustrates the order of
significance of the variables affecting the lipase production. The
present study concludes that the most significant variables affecting
lipase production were found to be palm oil, yeast extract, K2HPO4,
MgSO4 and CaCl2.", keywords = "lipase, submerged fermentation, statistical optimization, Trichoderma viride", volume = "5", number = "3", pages = "102-4", }
