Preliminary Study of Antimicrobial Activity against Escherichia coli and Probiotic Properties of Lactic Acid Bacteria Isolated from Thailand Fermented Foods

The lactic acid bacteria (LAB) were isolated from
10 samples of fermented foods (Sa-tor-dong and Bodo) in South
locality of Thailand. The 23 isolates of lactic acid bacteria were
selected, which were exhibited a clear zone and growth on MRS
agar supplemented with CaCO3. All of lactic acid bacteria were
tested on morphological and biochemical. The result showed that
all isolates were Gram’s positive, non-spore forming but only
10 isolates displayed catalase negative. The 10 isolates including
BD1 .1, BD 1.2, BD 2.1, BD2.2, BD 2.3, BD 3.1, BD 4.1, BD 5.2,
ST 4.1 and ST 5.2 were selected for inhibition activity
determination. Only 2 strains (ST 4.1 and BD 2.3) showed
inhibition zone on agar, when using Escherichia coli sp. as target
strain. The ST 4.1 showed highest inhibition zone on agar, which
was selected for probiotic property testing. The ST4.1 isolate
could grow in MRS broth containing a high concentration of
sodium chloride 6%, bile salts 7%, pH 4-10 and vary temperature
at 15-45°C.

• Phanwipa Pangsri
• Yawariyah Weahayee

• Lactic acid bacteria
• Probiotic
• Antimicrobial.

[1] Leroy, F. and De Vuyst, L. 2004. Lactic acid bacteria as
functional starter cultures for the food fermentation industry.
Trends in Food Science and Technology. 15,.78-67.
[2] Goldin, B.R. and Gorbach, S.L. 1992. Probiotics for humans In
Probiotics: the Scientific Basis, R. Fuller, editor Chapman and
Hall, London, UK, pp. .376-356.
[3] Ammor, M.S. and Mayo, B. 2007. Selection criteria for lactic acid
bacteria to be used as functional starter cultures in dry sausage
production: an update. Meat Science.76, 138-146.
[4] Hyronimus, B., Le Marrec, C., Hadi Sassi, A. and Deschamps,A. 2000.
Acid and bile tolerance of spore-forming lactic acid bacteria. Int. J. Food
Microbiol. 61: 193-197.
[5] Maragkoudakis, P.A.,Zoumpopoulou, G.,Miaris, C.,Kalantzopoulos,
G.,Pot, B. and E. Tsakalidou. 2006. Probiotic potential of Lactobacillus
strains isolated from dairy products. International Dairy Journal. 16 :
189-199.
[6] Onda, T., Yanagida, F., Uchimura, T., Tsuji, M., Ogino, S.,
Shinohara, T., & Yokotsuka, K. .2002. Widespread distribution of
the bacteriocin-producing lactic acid cocci in Miso-paste
products. Journal of Applied Microbiology, (4) 92 .695-705.
[7] Hwanhlema,N., Buradaleng S.,Wattanachant, S., Benjakul, S.,
Tani, A. and S. Maneerat. 2011. Isolation and screening of lactic
acid bacteria from Thai traditional fermented fish (Plasom) and
production of Plasom from selected strains. Food Control 22 :
401-407.
[8] Roth, L. A., & Keenan, D. 1971. Acid injury of Escherichia coli.
Canadian Journal of Microbiology. 17(8), 1005-1008.
[9] Przybylski, K. S., and Witter, L. D. 1979. Injury and recovery of
Escherichia coli after sublethal acidification. Applied and
Environmental Microbiology. 37(2), 261-265.
[10] Ahmad Faris Mohd Adnan, Irene K.P. Tan 2007. Isolation of lactic acid
bacteria from Malaysian foods and assessment of the isolates for
industrial potential. Volume 98, Issue 7, 1380–1385.
[11] Moser, S.A. and Savage, D.C. 2001. Bile salt hydrolase activity and
resistance to toxicity of conjugated bile salts are unrelated properties in
lactobacilli. Applied and Environmental Microbiology. 67, 3476-3480.
[12] Salminen, S. (1999). Probiotics: scientific support for use. Food
Technology. 53 (11), 66-77.