Optimization of Growth of Rhodobacter Sphaeroides Using Mixed Volatile Fatty Acidsby Response Surface Methodology
A combination of photosynthetic bacteria along with
anaerobic acidogenic bacteria is an ideal option for efficient
hydrogen production. In the present study, the optimum
concentration of substrates for the growth of Rhodobacter
sphaeroides was found by response surface methodology. The
optimum combination of three individual fatty acids was determined
by Box Behnken design. Increase of volatile fatty acid concentration
decreased the growth. Combination of sodium acetate and sodium
propionate was most significant for the growth of the organism. The
results showed that a maximum biomass concentration of 0.916 g/l
was obtained when the concentrations of acetate, propionate and
butyrate were 0.73g/l,0.99g/l and 0.799g/l, respectively. The growth
was studied under an optimum concentration of volatile fatty acids
and at a light intensity of 3000 lux, initial pH of 7 and a temperature
of 35°C.The maximum biomass concentration of 0.92g/l was
obtained which verified the practicability of this optimization.
[1] D. Das and T. N. Veziroglu, "Hydrogen production by biological
processes: a survey of literature", International Journal of Hydrogen
Energy, vol. 26, 2001, pp. 13-28.
[2] M. J. Barbosa, J. M. S. Rocha, J. Tramper and R.H. Wijffels, "Acetate as
a carbon source for hydrogen production by photosynthetic bacteria"
Journal of Biotechnology, vol.85, 2001, pp.25-33.
[3] K. Nath, M. Muthukumar, A. Kumar and D. Das, "Kinetics of twostage
fermentation process for the production of hydrogen", International
Journal of Hydrogen Energy, vol.33, 2008, pp.1195-1203
[4] H.H.P. Fang and H. Q. Yu, "Mesophilic acidification of gelatinaceous
wastewater", Journal of Biotechnology, vol. 93, 2002, pp. 99-108.
[5] H. G. Zhu, T. Wakayama, Y. Asada and J. Miyake, "Hydrogen
production by four cultures with participation by anoxygenic
phototrophic bacterium and anaerobic bacterium in the presence of
NH+4", International Journal of Hydrogen Energy, vol. 26, 2001,
pp.1149-1154.
[6] E. Fascetti, E. D-Addario, O. Todini and A. Robertiello, "Photosynthetic
hydrogen evolution with volatile organic acids derived from the
fermentation of source selected municipal solid wastes", International
Journal of Hydrogen Energy, vol.23, 1998, pp. 753-760.
[7] X. Y. Mao, J. Miyake and S.Kawamura, "Screening photosynthetic
bacteria for hydrogen production from organic acids", Journal of
Fermentation Technology, vol.64, 1986, 245-249.
[8] T. Arik, U. Gunduz, M. Yucel, L. Turker, V. Sediroglu and I. Eroglu,
"Photoproduction of hydrogen by Rhodobacter sphaeroides O.U.001",
In Hydrogen energy progress XI, Proceedings of the 11th WHEC, vol. 3.
Stuttgart, Germany: International Association for Hydrogen Energy,
1996, pp. 2417-24.
[9] I. Eroglu, K. Aslan, U. Gunduz, M. Yucel and L. Turker, "Substrate
consumption rates for hydrogen production by Rhodobacter sphaeroides
in a column photobioreactor" Journal of Biotechnology, vol. 70, 1999,
pp. 103-13.
[10] K. Sasikala, C.V. Ramana and P.R. Rao, "Environmental regulation for
optimal biomass yield and photoproduction of hydrogen by Rhodobacter
sphaeroides O.U. 001", International Journal of Hydrogen Energy, vol.
16, 1991, pp. 597-601.
[11] X.Y. Shi and H.Q. Yu, "Conversion of individual and mixed volatile
fatty acids to hydrogen by Rhodopseudomonas capsulata", International
Biodeterioration & Biodegradation, vol. 58, 2006, pp. 82-88.
[12] M. Kennedy and D. Krouse, "Strategies for improving fermentation
medium erformance: a review", Journal of Industrial Microbiogy
Biotechnolgy, vol. 23, 1999, pp. 456-475.
[13] J. T. Luftig and V.S. Jordan, "Design of experiments in quality
engineering", NewYork, McGraw-Hill; 1998.
[14] Jianlong Wang, Wei Wan, "Experimental design methods for
fermentative hydrogen production: A review", International journal of
hydrogen energy, vol. 34, 2008, pp. 235-244.
[15] G. E. P. Box AND E. W. Behnken, "Some new three level designs for
the study of quantitative variables", Technometrics, vol. 2, 1960, p.455-
475.
[1] D. Das and T. N. Veziroglu, "Hydrogen production by biological
processes: a survey of literature", International Journal of Hydrogen
Energy, vol. 26, 2001, pp. 13-28.
[2] M. J. Barbosa, J. M. S. Rocha, J. Tramper and R.H. Wijffels, "Acetate as
a carbon source for hydrogen production by photosynthetic bacteria"
Journal of Biotechnology, vol.85, 2001, pp.25-33.
[3] K. Nath, M. Muthukumar, A. Kumar and D. Das, "Kinetics of twostage
fermentation process for the production of hydrogen", International
Journal of Hydrogen Energy, vol.33, 2008, pp.1195-1203
[4] H.H.P. Fang and H. Q. Yu, "Mesophilic acidification of gelatinaceous
wastewater", Journal of Biotechnology, vol. 93, 2002, pp. 99-108.
[5] H. G. Zhu, T. Wakayama, Y. Asada and J. Miyake, "Hydrogen
production by four cultures with participation by anoxygenic
phototrophic bacterium and anaerobic bacterium in the presence of
NH+4", International Journal of Hydrogen Energy, vol. 26, 2001,
pp.1149-1154.
[6] E. Fascetti, E. D-Addario, O. Todini and A. Robertiello, "Photosynthetic
hydrogen evolution with volatile organic acids derived from the
fermentation of source selected municipal solid wastes", International
Journal of Hydrogen Energy, vol.23, 1998, pp. 753-760.
[7] X. Y. Mao, J. Miyake and S.Kawamura, "Screening photosynthetic
bacteria for hydrogen production from organic acids", Journal of
Fermentation Technology, vol.64, 1986, 245-249.
[8] T. Arik, U. Gunduz, M. Yucel, L. Turker, V. Sediroglu and I. Eroglu,
"Photoproduction of hydrogen by Rhodobacter sphaeroides O.U.001",
In Hydrogen energy progress XI, Proceedings of the 11th WHEC, vol. 3.
Stuttgart, Germany: International Association for Hydrogen Energy,
1996, pp. 2417-24.
[9] I. Eroglu, K. Aslan, U. Gunduz, M. Yucel and L. Turker, "Substrate
consumption rates for hydrogen production by Rhodobacter sphaeroides
in a column photobioreactor" Journal of Biotechnology, vol. 70, 1999,
pp. 103-13.
[10] K. Sasikala, C.V. Ramana and P.R. Rao, "Environmental regulation for
optimal biomass yield and photoproduction of hydrogen by Rhodobacter
sphaeroides O.U. 001", International Journal of Hydrogen Energy, vol.
16, 1991, pp. 597-601.
[11] X.Y. Shi and H.Q. Yu, "Conversion of individual and mixed volatile
fatty acids to hydrogen by Rhodopseudomonas capsulata", International
Biodeterioration & Biodegradation, vol. 58, 2006, pp. 82-88.
[12] M. Kennedy and D. Krouse, "Strategies for improving fermentation
medium erformance: a review", Journal of Industrial Microbiogy
Biotechnolgy, vol. 23, 1999, pp. 456-475.
[13] J. T. Luftig and V.S. Jordan, "Design of experiments in quality
engineering", NewYork, McGraw-Hill; 1998.
[14] Jianlong Wang, Wei Wan, "Experimental design methods for
fermentative hydrogen production: A review", International journal of
hydrogen energy, vol. 34, 2008, pp. 235-244.
[15] G. E. P. Box AND E. W. Behnken, "Some new three level designs for
the study of quantitative variables", Technometrics, vol. 2, 1960, p.455-
475.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:59011", author = "R.Sangeetha and T.Karunanithi", title = "Optimization of Growth of Rhodobacter Sphaeroides Using Mixed Volatile Fatty Acidsby Response Surface Methodology", abstract = "A combination of photosynthetic bacteria along with
anaerobic acidogenic bacteria is an ideal option for efficient
hydrogen production. In the present study, the optimum
concentration of substrates for the growth of Rhodobacter
sphaeroides was found by response surface methodology. The
optimum combination of three individual fatty acids was determined
by Box Behnken design. Increase of volatile fatty acid concentration
decreased the growth. Combination of sodium acetate and sodium
propionate was most significant for the growth of the organism. The
results showed that a maximum biomass concentration of 0.916 g/l
was obtained when the concentrations of acetate, propionate and
butyrate were 0.73g/l,0.99g/l and 0.799g/l, respectively. The growth
was studied under an optimum concentration of volatile fatty acids
and at a light intensity of 3000 lux, initial pH of 7 and a temperature
of 35°C.The maximum biomass concentration of 0.92g/l was
obtained which verified the practicability of this optimization.", keywords = "Biohydrogen, Response Surface Methodology,Rhodobacter sphaeroides, Volatile fatty acid", volume = "4", number = "1", pages = "97-4", }
