New Malate Dehydrogenase-Glutamate Oxaolacetate Aminotransferase Glutamate Oxaloacetate Aminotransferase Enzyme System from Cereals and its Bioengineering Application
Malate dehydrogenase-glutamate oxaloacetate
aminotransferase (MDh-GOAT) enzyme complex (the EC) was
isolated and purified from wheat and rise, their some main physicchemical
properties were studied. Michael-s constants of the EC
MDh-GOAT to malate, glutamate and NAD were investigated. This
kinetic results show a high relationship to glutamate. Taking into
account important role of the the EC in catabolism of glutamate – the
central amino acid of a nitric exchange, there is a sharp necessity of
deeper studying of this enzyme complex. Therefore the basic purpose
of the work is studying the basic physical and chemical properties of
this enzyme complex discovered by us, which would be very
important for understanding the mechanisms of reaction catalyzed by
the EC.
[1] B.G. Forde, P. J. Lea, "Glutamate in plants: metabolism, regulation, and
signaling", Journal of Experimental Botany, vol. 58, issue 9, pp. 2339-
2358, 2007.
[2] J. L Bailey, Techniques in protein chemistry, Elsevier Scientific
Publishing Co., Inc., New York, N.Y. p. 294-295, 1962.
[3] M.K. Gilmanov, O.V. Fursov, A.P. Frantsev, Methods of clearing and
studying of enzymes of plants, Alma-ata, 1981, pp. 91-92. (In russian).
[4] A. Almeida, S.J. Heales, J.P. Bola├▒os, J.M. Medina, "Glutamate
neurotoxicity is associated with nitric oxide-mediated mitochondrial d",
Brain Res, vol. 790, pp. 209-216, 1998.
[1] B.G. Forde, P. J. Lea, "Glutamate in plants: metabolism, regulation, and
signaling", Journal of Experimental Botany, vol. 58, issue 9, pp. 2339-
2358, 2007.
[2] J. L Bailey, Techniques in protein chemistry, Elsevier Scientific
Publishing Co., Inc., New York, N.Y. p. 294-295, 1962.
[3] M.K. Gilmanov, O.V. Fursov, A.P. Frantsev, Methods of clearing and
studying of enzymes of plants, Alma-ata, 1981, pp. 91-92. (In russian).
[4] A. Almeida, S.J. Heales, J.P. Bola├▒os, J.M. Medina, "Glutamate
neurotoxicity is associated with nitric oxide-mediated mitochondrial d",
Brain Res, vol. 790, pp. 209-216, 1998.
@article{"International Journal of Medical, Medicine and Health Sciences:54861", author = "Zhanar S. Kudiyarova and Zhanar K. Rakhmetova and L. K. Bekbayeva and N. Z. Omirbekova and M. K. Gilmanov", title = "New Malate Dehydrogenase-Glutamate Oxaolacetate Aminotransferase Glutamate Oxaloacetate Aminotransferase Enzyme System from Cereals and its Bioengineering Application", abstract = "Malate dehydrogenase-glutamate oxaloacetate
aminotransferase (MDh-GOAT) enzyme complex (the EC) was
isolated and purified from wheat and rise, their some main physicchemical
properties were studied. Michael-s constants of the EC
MDh-GOAT to malate, glutamate and NAD were investigated. This
kinetic results show a high relationship to glutamate. Taking into
account important role of the the EC in catabolism of glutamate – the
central amino acid of a nitric exchange, there is a sharp necessity of
deeper studying of this enzyme complex. Therefore the basic purpose
of the work is studying the basic physical and chemical properties of
this enzyme complex discovered by us, which would be very
important for understanding the mechanisms of reaction catalyzed by
the EC.", keywords = "Malate dehydrogenase-glutamate oxaloacetate aminotransferase, enzyme complex, glutamate.", volume = "2", number = "8", pages = "266-3", }