Laboratory Scale Extraction of Sugar Cane using High Electric Field Pulses

The aim of this study was to extract sugar from sugarcane using high electric field pulse (HELP) as a non-thermal cell permeabilization method. The result of this study showed that it is possible to permeablize sugar cane cells using HELP at very short times (less than 10 sec.) and at room temperature. Increasing the field strength (from 0.5kV/cm to 2kV/cm) and pulse number (1 to 12) led to increasing the permeabilization of sugar cane cells. The energy consumption during HELP treatment of sugar cane (2.4 kJ/kg) was about 100 times less compared to thermal cell disintegration at 85 <=C (about 271.7 kJ/kg). In addition, it was possible to extract sugar cane at a moderate temperature (45 <=C) using HELP pretreatment. With combination of HELP pretreatment followed by thermal extraction at 75 <=C, extraction resulted in up to 3% more sugar (on the basis of total extractable sugar) compared to samples without HELP pretreatment.




References:
[1] D. Knorr, M. Geulen, T. Grahl, and W. Sitzmann, W. "Food application of high electric field pulses,". Trends Food Sci Technol.,
vol. 5, pp.71-75, 1994.
[2] M. Bazhal.and E. Vorobiev, "Electrical treatment of apple cossettes for intensifying juice processing,". J. Sci. Food Agric. vol. 80, pp. 1668-
1674, 2000.
[3] M. Fincan, F. DeVito and P. Dejmek, "Pulsed electric field treatment for
solid-liquid extraction of red beetroot pigment," J. Food Eng. vol. 64, pp. 381-388, 2004.
[4] W. Sitzmann, and E.W. Munch, "Das ELCRACK Verfahren: Ein neues
Verfahren f├╝r Verarbeitung tierischer Rohstoffe, " Die
Fischmehlindustrie, vol. 40, pp. 22-28, February 1988.
[5] H. Dovenspeck, "Verfahren und Vorrichtung yur Gewinnung der
einyelne Phasen aus dispersen Systemen, " DE 1, pp. 237-541, 1960.
[6] H. Dovenspeck, 1961. "Influencing cells and cell walls by electrostatic
impulses, " Fleischwirtschaft. vol. 13, pp. 968-987, Dec. 1961.
[7] B L.Flaumenbaum, "Anwendung der Electroplasmolyse bei der
Herstellung von Fruchtsaften, " Flussiges Obst. vol. 35, pp. 19-22, 1968.
[8] C. Schultheiss, H.J. Bluhm, H.G. Mayer, M. Kern, T. Michelberger, and
G. Witte, "Processing of sugar beets with pulsed electric fields," IEEE
Trans. Plasma Sci., vol. 30, pp. 1547-1551, April 2002.
[9] K.M. Bliesener, D. Miehe, K. Buchholz, "Verfahrensentwicklungen zur
Schnitzelentwesserung, ". Zuckerindustre, vol. 16, pp. 978-986, 1991.
[10] M.N. Eshtiaghi and D. Knorr, "High electric field pulse treatment:
Potential for sugar beet processing," J. Food Eng. vol. 52, pp. 265-272, 2002.
[11] M.N. Eshtiaghi and D. Knorr, D. "Process for treatment of sugar beet,
European Patent," Patent-Nr. :EP 99923708. 1999.
[12] J. Kuldiloke,.M.N. Eshtiaghi, C. Neatpisarnvanit and T. Uan-On, T.
"Application of high electric field pulses for sugar cane processing". KMITL Sci. Tech. J., vol. 8, pp. 75-83, June 2008.