Isolation and Molecular Identification of Two Fungal Strains Capable of Degrading Hydrocarbon Contaminants on Saudi Arabian Environment
In the vicinity of red sea about 15 fungi species were
isolated from oil contaminated sites. On the basis of aptitude to
degrade the crude oil and DCPIP assay, two fungal isolates were
selected amongst 15 oil degrading strains. Analysis of ITS-1, ITS-2
and amplicon pyrosequencing studies of fungal diversity revealed
that these strains belong to Penicillium and Aspergillus species. Two
strains that proved to be the most efficient in degrading crude oil was
Aspergillus niger (54%) and Penicillium commune (48%) Subsequent
to two weeks of cultivation in BHS medium the degradation rate
were recorded by using spectrophotometer and GC-MS. Hence, it is
cleared that these fungal strains has capability of degradation and can
be utilize for cleaning the Saudi Arabian environment.
[1] Plohl K, Leskovsˇek H, Bricelj M (2002) Biological degradation of
motor oil in water. Acta Chim Slov 49:279–289.
[2] Bumpus JA (1989) Biodegradation of polycyclic aromatic hydrocarbons
by Phanerochaete chrysosporium. Appl Environ Microbiol 55:154–158.
[3] Clemente AR, Anazawa TA, Durrant LR (2001) Biodegradation of
polycyclic aromatic hydrocarbons by soil fungi. Braz J Microbiol
32:255–261.
[4] Cerniglia CE, Sutherland JB (2001) Bioremediation of polycyclic
aromatic hydrocarbons by ligninolytic and non-ligninolytic fungi. In:
Gadd GM (ed) Fungi in bioremediation. Cambridge University Press,
Cambridge, pp 136–187.
[5] Bumpus JA, Tien M, Wright D, Aust SD (1985) Oxidation of persistent
environmental pollutants by a white rot fungus. Science 228:1434–1436.
[6] Novotny C, Erbanova P, Sasek V, Kubatova A, Cajthaml T, Lang E et al
(1999) Extracellular oxidative enzyme production and PAH removal in
soil by exploratory mycelium of white rot fungi. Biodegradation
10:159–168.
[7] April TM, Fought JM, Currah RS (2000) Hydrocarbon-degrading
filamentous fungi isolated from flare pit soils in northern and western
Canada. Can J Microbiol 46:38–49.
[8] Prenafeta-Boldu´ FX, Kuhn A, Luykx D, Anke H, van Groenestijn JW,
de Bont JAM (2001) Isolation and characterisation of fungi growing on
volatile aromatic hydrocarbons as their sole carbon and energy source.
Mycol Res 105:477–484.
[9] Saraswathy A, Hallberg R (2002) Degradation of pyrene by indigenous
fungi from a former gaswork site. FEMS Microbiol Lett 210:227–232.
[10] Mesyami P, Baheri M (2003) Pre-screening of fungi and bulking agents
for contaminated soil bioremediation. Adv Environ Res 7:881–887.
[11] Yamada K, Mukumoto H, Katsuyama Y, Tani Y (2002) Degradation of
long-chain alkanes by a polyethelene-degrading fungus, Penicillium
simplicissimum YK. Enzyme Microb Technol 30:828–831.
[12] Santos EO, Centeno da Rosa CF, dos Passos CT, Sanzo AVL, de
Medeiros Burkert JF, Kalil SJ et al (2008) Pre-screening of filamentous
fungi isolated from a contaminated site in Southern Brazil for
bioaugmentation purposes. Afr J Biotechnol 7(9):1314–1317.
[13] Adekunle, I.M., Adetunji M.T., Gbadebo A.M., and Banjoko O.B.
(2007). Assessment of Groundwater Quality in a Typical Rural
Settlement in Southwest, Nigeria”. Int. Journal of Environment and
Public Health. 4(4): 307-318.
[14] Anupama M, Padma S.(2009). Studies on biodegradaction of crude oil
by Aspergillus niger, The South Pacific Journal of Natural Science,
27:57-60
[15] Esteve-Zarzoso B., Belloch C., Uruburu F.and A.Querol(1999).
International Journal of Systematic Bacteriology 49: 329 (1999).
[16] White T. J., Bruns T., Lee S. and J.Taylor, in: A. Innis, Gelfand D. H.
and J. J. Sninsky (eds.), (1990). PCR Protocols, Academic Press, San
Diego, USA, pp. 315-322.
[17] Tamura, K., Nei, M., Kumar, S. (2004). Prospects for inferring very
large phylogenies by using the neighbor-joining method. Proc. Natl.
Acad. Sci. U S A. 101, 11030-11035.
[18] Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: Molecular
Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol.
Biol. Evol. 24, 1596-1599.
[19] Hanson KG, Desai JD, Desai AJ (1993). A rapid and simple screening
technique for potential crude oil degrading microorganisms. Biotechnol
Tech 7: 745-748.
[20] Guo-liang Zhang, Yue-ting Wu, Xin-ping Qian, Qin Meng(2005).
Biodegradation of crude oil by Pseudomonas aeruginosa in the presence
of rhamnolipids, J Zhejiang Univ Sci B. 6: 725–730.
[21] Joo, M.H., Kim, J.Y. (2013). Characteristics of crude oil biodegradation
by biosurfactant-producing bacterium Bacillus subtilis JK-1. J. Korean
Soc. Appl. Biol. Chem. 56, 193–200.
[22] Al-Nasrawi Hussein (2012). Biodegradation of Crude Oil by Fungi
Isolated from Gulf of Mexico. J Bioremed Biodegrad 3:147.
[23] Leahy, J.G., Tracy, K.D., Eley, M.H. (2003). Degradation of mixtures of
aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbondegrading
bacteria. FEMS Microbiol. Ecol. 43:271-276.
[24] Gesinde AF, Agbo EB, Agho MO, Dike EFC (2008) Bioremediation of
Some Nigerian and Arabian Crude Oils by Fungal Isolates. Int Jor P App
Scs 2: 37-44.
[25] Obire O, Anyanwu E.C. (2009). Impact of various concentrations of
crude oil on fungal populations of soil. Int J Environ Sci Technol 6: 211-
218.
[26] De N, Bello YM, Saleh M (2000) Biodegradation of crude oil by
Fusarium sp. and Trichoderma sp. Isolated from oil contaminated soil in
different auto mechanic garages. Waste and Management 18 (6).
[27] Bartha R (1977). The microbiology of aquatic oil spills. Adv Appl
Microbiol 22: 225-266.
[28] Norman, R.S., Moeller, P., McDonald, T.J., Morris, P.J. (2004). Effect
of Pyocyanin on a Crude-Oil-Degrading Microbial Community. Appl.
Environ. Microbiol. 70: 4004–4011.
[29] Abioye OP, Akinsola, RO, Aransiola SA, Damisa D.(2013).
Biodegradation of crude oil by saccharomyces cerevisiae isolated from
fermented zobo (locally fermented beverage in Nigeria). Pak J Biol sci .
15; 16:2058-61.
[1] Plohl K, Leskovsˇek H, Bricelj M (2002) Biological degradation of
motor oil in water. Acta Chim Slov 49:279–289.
[2] Bumpus JA (1989) Biodegradation of polycyclic aromatic hydrocarbons
by Phanerochaete chrysosporium. Appl Environ Microbiol 55:154–158.
[3] Clemente AR, Anazawa TA, Durrant LR (2001) Biodegradation of
polycyclic aromatic hydrocarbons by soil fungi. Braz J Microbiol
32:255–261.
[4] Cerniglia CE, Sutherland JB (2001) Bioremediation of polycyclic
aromatic hydrocarbons by ligninolytic and non-ligninolytic fungi. In:
Gadd GM (ed) Fungi in bioremediation. Cambridge University Press,
Cambridge, pp 136–187.
[5] Bumpus JA, Tien M, Wright D, Aust SD (1985) Oxidation of persistent
environmental pollutants by a white rot fungus. Science 228:1434–1436.
[6] Novotny C, Erbanova P, Sasek V, Kubatova A, Cajthaml T, Lang E et al
(1999) Extracellular oxidative enzyme production and PAH removal in
soil by exploratory mycelium of white rot fungi. Biodegradation
10:159–168.
[7] April TM, Fought JM, Currah RS (2000) Hydrocarbon-degrading
filamentous fungi isolated from flare pit soils in northern and western
Canada. Can J Microbiol 46:38–49.
[8] Prenafeta-Boldu´ FX, Kuhn A, Luykx D, Anke H, van Groenestijn JW,
de Bont JAM (2001) Isolation and characterisation of fungi growing on
volatile aromatic hydrocarbons as their sole carbon and energy source.
Mycol Res 105:477–484.
[9] Saraswathy A, Hallberg R (2002) Degradation of pyrene by indigenous
fungi from a former gaswork site. FEMS Microbiol Lett 210:227–232.
[10] Mesyami P, Baheri M (2003) Pre-screening of fungi and bulking agents
for contaminated soil bioremediation. Adv Environ Res 7:881–887.
[11] Yamada K, Mukumoto H, Katsuyama Y, Tani Y (2002) Degradation of
long-chain alkanes by a polyethelene-degrading fungus, Penicillium
simplicissimum YK. Enzyme Microb Technol 30:828–831.
[12] Santos EO, Centeno da Rosa CF, dos Passos CT, Sanzo AVL, de
Medeiros Burkert JF, Kalil SJ et al (2008) Pre-screening of filamentous
fungi isolated from a contaminated site in Southern Brazil for
bioaugmentation purposes. Afr J Biotechnol 7(9):1314–1317.
[13] Adekunle, I.M., Adetunji M.T., Gbadebo A.M., and Banjoko O.B.
(2007). Assessment of Groundwater Quality in a Typical Rural
Settlement in Southwest, Nigeria”. Int. Journal of Environment and
Public Health. 4(4): 307-318.
[14] Anupama M, Padma S.(2009). Studies on biodegradaction of crude oil
by Aspergillus niger, The South Pacific Journal of Natural Science,
27:57-60
[15] Esteve-Zarzoso B., Belloch C., Uruburu F.and A.Querol(1999).
International Journal of Systematic Bacteriology 49: 329 (1999).
[16] White T. J., Bruns T., Lee S. and J.Taylor, in: A. Innis, Gelfand D. H.
and J. J. Sninsky (eds.), (1990). PCR Protocols, Academic Press, San
Diego, USA, pp. 315-322.
[17] Tamura, K., Nei, M., Kumar, S. (2004). Prospects for inferring very
large phylogenies by using the neighbor-joining method. Proc. Natl.
Acad. Sci. U S A. 101, 11030-11035.
[18] Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: Molecular
Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol.
Biol. Evol. 24, 1596-1599.
[19] Hanson KG, Desai JD, Desai AJ (1993). A rapid and simple screening
technique for potential crude oil degrading microorganisms. Biotechnol
Tech 7: 745-748.
[20] Guo-liang Zhang, Yue-ting Wu, Xin-ping Qian, Qin Meng(2005).
Biodegradation of crude oil by Pseudomonas aeruginosa in the presence
of rhamnolipids, J Zhejiang Univ Sci B. 6: 725–730.
[21] Joo, M.H., Kim, J.Y. (2013). Characteristics of crude oil biodegradation
by biosurfactant-producing bacterium Bacillus subtilis JK-1. J. Korean
Soc. Appl. Biol. Chem. 56, 193–200.
[22] Al-Nasrawi Hussein (2012). Biodegradation of Crude Oil by Fungi
Isolated from Gulf of Mexico. J Bioremed Biodegrad 3:147.
[23] Leahy, J.G., Tracy, K.D., Eley, M.H. (2003). Degradation of mixtures of
aromatic and chloroaliphatic hydrocarbons by aromatic hydrocarbondegrading
bacteria. FEMS Microbiol. Ecol. 43:271-276.
[24] Gesinde AF, Agbo EB, Agho MO, Dike EFC (2008) Bioremediation of
Some Nigerian and Arabian Crude Oils by Fungal Isolates. Int Jor P App
Scs 2: 37-44.
[25] Obire O, Anyanwu E.C. (2009). Impact of various concentrations of
crude oil on fungal populations of soil. Int J Environ Sci Technol 6: 211-
218.
[26] De N, Bello YM, Saleh M (2000) Biodegradation of crude oil by
Fusarium sp. and Trichoderma sp. Isolated from oil contaminated soil in
different auto mechanic garages. Waste and Management 18 (6).
[27] Bartha R (1977). The microbiology of aquatic oil spills. Adv Appl
Microbiol 22: 225-266.
[28] Norman, R.S., Moeller, P., McDonald, T.J., Morris, P.J. (2004). Effect
of Pyocyanin on a Crude-Oil-Degrading Microbial Community. Appl.
Environ. Microbiol. 70: 4004–4011.
[29] Abioye OP, Akinsola, RO, Aransiola SA, Damisa D.(2013).
Biodegradation of crude oil by saccharomyces cerevisiae isolated from
fermented zobo (locally fermented beverage in Nigeria). Pak J Biol sci .
15; 16:2058-61.
@article{"International Journal of Biological, Life and Agricultural Sciences:71495", author = "Amr A. El Hanafy and Yasir Anwar and Saleh A. Mohamed and Saleh Mohamed Saleh Al-Garni and Jamal S. M. Sabir and Osama A. H. Abu Zinadah and Mohamed Morsi Ahmed", title = "Isolation and Molecular Identification of Two Fungal Strains Capable of Degrading Hydrocarbon Contaminants on Saudi Arabian Environment", abstract = "In the vicinity of red sea about 15 fungi species were
isolated from oil contaminated sites. On the basis of aptitude to
degrade the crude oil and DCPIP assay, two fungal isolates were
selected amongst 15 oil degrading strains. Analysis of ITS-1, ITS-2
and amplicon pyrosequencing studies of fungal diversity revealed
that these strains belong to Penicillium and Aspergillus species. Two
strains that proved to be the most efficient in degrading crude oil was
Aspergillus niger (54%) and Penicillium commune (48%) Subsequent
to two weeks of cultivation in BHS medium the degradation rate
were recorded by using spectrophotometer and GC-MS. Hence, it is
cleared that these fungal strains has capability of degradation and can
be utilize for cleaning the Saudi Arabian environment.
", keywords = "Fungal strains, hydrocarbon contaminants, molecular
identification, biodegradation, GC-MS.", volume = "9", number = "12", pages = "1215-4", }
