Investigation of Anti-Inflammatory, Antipyretic and Analgesic Effect of Yemeni Sidr Honey
Traditionally, Yemini Sidr honey has been reported to
cure liver problems, stomach ulcers, and respiratory disorders. In this
experiment, we evaluated Yemeni Sidr honey for its ability to protect
inflammations caused by acetic acid and formalin -induced writhing,
carrageenan and histamine-induced paw oedema in experimental rat
model. Hyperpyrexia, membrane stabilizing activity, and
phytochemical screening of the honey was also examined. Yemini
Sidr Honey at (100, 200 and 500 mg/kg) exhibited a concentration
dependant inhibition of acetic acid induced and formalin induced
writhing, paw oedema induced by carrageenan & histamine, and
hyperpyrexia induced by brewer's yeast, it also inhibited membrane
stabilizing activity. Phytochemical screenings of the honey reveal the
presence of flavonoids, steroid, alkaloids, saponins and tannins. This
study suggested that Yemeni Sidr honey possess very strong antiinflammatory,
analgesic and antipyretic effects and these effects
would be a result of the phytochemicals present.
[1] Al-Mamary, M., Al-Meeri, A., AL-Habori, M., (2002). Antioxidant
activities and total phenolics of different types of honey. Nutrition
Research, 22, 1041-1047.
[2] Basualdo, C., Sgroy, V., Finola, M. S., Marioli, J. M., (2007).
Comparison of the antibacterial activity of honey from different
provenance against bacteria usually isolated from skin wounds.
Veterinary Microbiology. 124, 375-381.
[3] Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P., Estevinho, L.,
(2009). Physicochemical, microbiological and antimicrobial properties
of commercial honeys from Portugal.Food and Chemical Toxicology.
48, 544-548.
[4] White, J.W. Jr., (1975).Composition of Honey.In : Honey : a
comprehensive survey. (Crane E, ed) Heinemann,London, 180-194
[5] Collier, H.O.J., Dinneen, L.C, Johnson,C.A., Schneider, C., (1968).The
abdominal constriction response and its suppression by analgesic drugs
in the mouse. British Journal of pharmacology and chemotherapy
32,295-310.
[6] Fontenele,J.B., Viana, G.S.B., Xavier-Filho,J., Alencar, J.W., (1996).
Anti-inflammatory and analgesic activity of a water-soluble fraction
from shark cartilage.Brazilian Journal of Medicinal and Biology
Research 29, 643-464.
[7] Koster, R., Anderson, M., De Beer, E.J., (1959). Acetic acid and
analgesic screening. Proceedings of the Society for Experimental
Biology and Medicine 18, 412-415.
[8] Santos, A.R., Calixto,J.B.,(1997). Further evidence for the involvement
of tachykinin receptor subtypes in formalin and capsaicin models of pain
in mice. Neuropeptides 31,381- 389.
[9] Vilela, F.C., Padilha, M.M., Dos Santos-E-Silva, L., Alves-da-silva, G.,
Giusti-Paiva, A., (2009). Evaluation of the antinociceptive activity of
extracts of sonchusoleraceus L. in mice. Journal of Enthopharmacology
124, 306-310.
[10] Winter, C.A., Risley, E.A., Nuss, G.W., (1962).Carrageenan-induced
oedema in hind paw of the rat as an assay for anti-inflammatory drugs.
Proceeding of the society for Experimental Biology and Medicine
111,544-547.
[11] Birch, P.J., Harrison, S.M., Hayes, A.G., Rogers, H.,Tyers, M.B.,
(1992). The non-peptide NK1 receptor antagonist,(+/)-CP-96,345,
produces antinociceptive and anti-edema effects in the rat. Br. J.
Pharmacol.105,508-510.
[12] Al-Ghamdi, M.S., (2001). The anti-inflammatory, analgesic and
antipyretic activity of Nigella sativa. J Ethnopharmacol ;76:45-8.
[13] Smith, P. K. & Hamburger, W. E. (1935). The ratio of the Toxicity of
Acetanilid to its Antipyretic Activity in Rat. J. Pharmacol. Exp. Ther.,
54, 346-351.
[14] Makonnen, E.,Makonnen, A.,Debella, L.,Zerihun, D.,Abebe,F and Teka,
F, (2003). Antipyretic properties of the aqueous and ethanol extracts of
the leaves of Ocimum suave and Ocimumlamiifolium in mice, Journal of
Ethnopharmacology 88, pp. 85-91.
[15] Sulaimana,M.R.,.Perimala,E.K.,Akhtarb,M.N.,Mohamada,A.S.
,Khalida,M.H.,Tasripa,N.A.,Mokhtarb,F.,Zakariaa,Z.A.,Lajisb,N.H.
andIsrafa, D.A., (2010). Anti-inflammatory effect of zerumbone on
acute and chronic inflammation models in mice. Fitoterapia 81(7) 855-
858.
[16] Sadique, J., Al-Raobah, N.A., Baghaith, M.F., El-Gindy, A.R., (1989).
The bioactivity of certain medicinal plants on the stabilization of red
blood cell membrane system.Fitoterapia LX, 525-532.
[17] Oyedapo, O.O., Famurewa, A.J., (1995). Antiprotease and membrane
stabilizing activity of extracts of Fagarazanthoxyloides,
Olaxsubscorpioides and Tetrapleuratetraptera. International Journal of
Pharmacognosy33, 65-69.
[18] Odebiyi, O.O., Sofowora, E.A., (1978). Phytochemical screening of
Nigerian medicinal plants II. Lloydia, Ohio 41, 234-
246.Kumar,A.,Llavarasan,R.,Jayachandran,T.,Decarama,M.,Aravindhan
,P.,Padmanabhan,N.and Krishan,M.R.V.,(2009). Phytochemical
Investigation on Tropical plants,Syzgiumcumnini from Kattuppalayam
,Erode District,TamilNaclu,south India. Pakistan Journal of Nutrition,
8(1), 83-85. Additional reference
[19] Vidyalashmi, K., Kamalakannan,P., Viswanathan, S., Ramaswamy, S.,
(2010).Antinociceptive effect of certain dihydroxy flavones in mice.
Pharmacology, Biochemistry and Behavior 96, 1-6.
[20] Collier , H.O., Dinneen, L.C.,Johnson,C.A., Schneider, C., ( 1968).The
abdominal constriction response and its suppression by analgesic drugs
in the mouse. British Journal of pharmacology and chemotherapy 32,
295-310.
[21] Deradt, R., Jougney, S., Benzoni, J., Peterfalvi, M., (1980).Release of
prostaglandins E and F in an algogenic reaction and its inhibition
.European journal of pharmacology 61, 17-24.
[22] Martinez, V., Thakur, S., Mogil, J.S., Tache, Y., Mayer, E.A.,
1999.Differential effects of chemical and mechanical colonic irritation
on behavioral pain response to intraperitoneal acetic in mice. Pain 81,
163-185.
[23] Julius, D., Basbaum, A.I., (2001). Molecular mechanism of nociception
.Nature 413, 203-210.
[24] Hunskaar, S., Hole, K., (1987) .The formalin test in mice:dissociation
between inflammatory and non-inflammatory pain. Pain 30, 130-114.
[25] Tjolsen, A., Berge, O.G., Hunskaar, S., Roslan, J.H., Hole,K.,
(1992).The formain test: an evaluation of the method.Pain 51, 5-17.
[26] Crunkhorn P, Meacock S. C. R., (1971). Mediators of the inflammation
induced in the rat paw by carrageenan. Br J Pharmacol. 42(3): 392-402.
[27] Van Arman, C. G., Begany A. J., Miller, L. M. andPless, H. H. (2002).
Some details of the inflammations caused by yeast and carrageenin (with
appendix on kinetics of the reaction). J. Neurosci. 22:6208-6217.
[28] Ozaki, Y., (1990). Antiinflammatory effect of Curcuma xanthorrhiza
ROXB.and its active principles . Chemical and pharmaceutical
bulletin.1045-1048 (Tokyo).
[29] Babior,B.M, Kipnes,R.S, Curnutte, J.T. (1973). The production by
leucocytes of superoxide, a potential bactericidal agent. J Clin Invest,
52: 741-4.
[30] Sethi, S., Singh, M.P., Dikshit, M., (2000). Mechanism involved in the
augmentation of arachidonic acid-induced free radicalgeneration from
rat neutrophils following hypoxia-reoxygenation. Trom Res 98:445-50.
[31] Latenti, A., Ianaro, A., Moncada, S., Di Rosa, M.,(1992).Modulation of
acute inflammation by endogenous nitric oxide. Eur J Pharmacol
211:177-86.
[32] Di Rosa, M., Giroud, J.P., Willoughby, D.A., (1971).Studies of
mediators of the acute inflammatory response induced in rats indifferent
site by carrageenan and turpentine. J Pathol 104: 15-21.
[33] Abramson, S.B., Weissman, G., (1989). The mechanism of action of
nonsteroidal anti-inflammatory drugs. Arthritis Rheum 32: 1-9.
[34] McCord, J.M., (1974). Free radicals and inflammation: Protection of
synovial fluid by superoxidedismutase. Science 185: 529-31.
[35] Salvemini, D., Wang, Z.O., Bourdon, D.M., Stern, M.K., Currie, M.G.,
Manning, P.T.,(1996). Evidence of peroxynitrite involvement in the
carrageenan-induced rat pawoedema.Eur J pharmacol 303: 217-20.
[36] Cuzzocrea, S., Zingarelli, B., Gilard, E., Hake, P., Salzman, A.L., Szabo,
C.,(1998). Antiinflammatory effect of mercapoethylguanidine, a
combined inhibitor of nitricoxide synthase and peroxynitrite scavenger,
in carrageenan-induced models of inflammation .Free RadicBiolMed 24:
450-9.
[37] Al-Ghamdi, M.S.,(2001).The anti-inflammatory ,analgesic and
antipyretic activity of Nigella sativa .J Ethanopharmacol ,76:45-8 .
[38] Hess, S.M., Millonig, R.C., (1972) .Assay for anti-inflammatory drugs
.In: Lepow, I.H., Ward,P.A., (Eds.),Inflammation ,Mechanisms and
control. Publication of Academic Press, London.
[39] Menuka, A.,Thabrew, I., Lakshmi, A., Shiroma, H., (2010). Antiinflammatory
activity of Trichosanthescucumerina Linn.in rats .Journal
of Ethnopharmacology 131:538-543.
[40] Aitadafoun, M., Mounieri, C., Heyman, S.F., Binistic, C., Bon, C.,
Godfroid, J., (1996).4-Alkoxybenzamidines as new potent
phospholipase A2 inhibitors.Biochemical pharmacology 51, 737-742.
[1] Al-Mamary, M., Al-Meeri, A., AL-Habori, M., (2002). Antioxidant
activities and total phenolics of different types of honey. Nutrition
Research, 22, 1041-1047.
[2] Basualdo, C., Sgroy, V., Finola, M. S., Marioli, J. M., (2007).
Comparison of the antibacterial activity of honey from different
provenance against bacteria usually isolated from skin wounds.
Veterinary Microbiology. 124, 375-381.
[3] Gomes, S., Dias, L. G., Moreira, L. L., Rodrigues, P., Estevinho, L.,
(2009). Physicochemical, microbiological and antimicrobial properties
of commercial honeys from Portugal.Food and Chemical Toxicology.
48, 544-548.
[4] White, J.W. Jr., (1975).Composition of Honey.In : Honey : a
comprehensive survey. (Crane E, ed) Heinemann,London, 180-194
[5] Collier, H.O.J., Dinneen, L.C, Johnson,C.A., Schneider, C., (1968).The
abdominal constriction response and its suppression by analgesic drugs
in the mouse. British Journal of pharmacology and chemotherapy
32,295-310.
[6] Fontenele,J.B., Viana, G.S.B., Xavier-Filho,J., Alencar, J.W., (1996).
Anti-inflammatory and analgesic activity of a water-soluble fraction
from shark cartilage.Brazilian Journal of Medicinal and Biology
Research 29, 643-464.
[7] Koster, R., Anderson, M., De Beer, E.J., (1959). Acetic acid and
analgesic screening. Proceedings of the Society for Experimental
Biology and Medicine 18, 412-415.
[8] Santos, A.R., Calixto,J.B.,(1997). Further evidence for the involvement
of tachykinin receptor subtypes in formalin and capsaicin models of pain
in mice. Neuropeptides 31,381- 389.
[9] Vilela, F.C., Padilha, M.M., Dos Santos-E-Silva, L., Alves-da-silva, G.,
Giusti-Paiva, A., (2009). Evaluation of the antinociceptive activity of
extracts of sonchusoleraceus L. in mice. Journal of Enthopharmacology
124, 306-310.
[10] Winter, C.A., Risley, E.A., Nuss, G.W., (1962).Carrageenan-induced
oedema in hind paw of the rat as an assay for anti-inflammatory drugs.
Proceeding of the society for Experimental Biology and Medicine
111,544-547.
[11] Birch, P.J., Harrison, S.M., Hayes, A.G., Rogers, H.,Tyers, M.B.,
(1992). The non-peptide NK1 receptor antagonist,(+/)-CP-96,345,
produces antinociceptive and anti-edema effects in the rat. Br. J.
Pharmacol.105,508-510.
[12] Al-Ghamdi, M.S., (2001). The anti-inflammatory, analgesic and
antipyretic activity of Nigella sativa. J Ethnopharmacol ;76:45-8.
[13] Smith, P. K. & Hamburger, W. E. (1935). The ratio of the Toxicity of
Acetanilid to its Antipyretic Activity in Rat. J. Pharmacol. Exp. Ther.,
54, 346-351.
[14] Makonnen, E.,Makonnen, A.,Debella, L.,Zerihun, D.,Abebe,F and Teka,
F, (2003). Antipyretic properties of the aqueous and ethanol extracts of
the leaves of Ocimum suave and Ocimumlamiifolium in mice, Journal of
Ethnopharmacology 88, pp. 85-91.
[15] Sulaimana,M.R.,.Perimala,E.K.,Akhtarb,M.N.,Mohamada,A.S.
,Khalida,M.H.,Tasripa,N.A.,Mokhtarb,F.,Zakariaa,Z.A.,Lajisb,N.H.
andIsrafa, D.A., (2010). Anti-inflammatory effect of zerumbone on
acute and chronic inflammation models in mice. Fitoterapia 81(7) 855-
858.
[16] Sadique, J., Al-Raobah, N.A., Baghaith, M.F., El-Gindy, A.R., (1989).
The bioactivity of certain medicinal plants on the stabilization of red
blood cell membrane system.Fitoterapia LX, 525-532.
[17] Oyedapo, O.O., Famurewa, A.J., (1995). Antiprotease and membrane
stabilizing activity of extracts of Fagarazanthoxyloides,
Olaxsubscorpioides and Tetrapleuratetraptera. International Journal of
Pharmacognosy33, 65-69.
[18] Odebiyi, O.O., Sofowora, E.A., (1978). Phytochemical screening of
Nigerian medicinal plants II. Lloydia, Ohio 41, 234-
246.Kumar,A.,Llavarasan,R.,Jayachandran,T.,Decarama,M.,Aravindhan
,P.,Padmanabhan,N.and Krishan,M.R.V.,(2009). Phytochemical
Investigation on Tropical plants,Syzgiumcumnini from Kattuppalayam
,Erode District,TamilNaclu,south India. Pakistan Journal of Nutrition,
8(1), 83-85. Additional reference
[19] Vidyalashmi, K., Kamalakannan,P., Viswanathan, S., Ramaswamy, S.,
(2010).Antinociceptive effect of certain dihydroxy flavones in mice.
Pharmacology, Biochemistry and Behavior 96, 1-6.
[20] Collier , H.O., Dinneen, L.C.,Johnson,C.A., Schneider, C., ( 1968).The
abdominal constriction response and its suppression by analgesic drugs
in the mouse. British Journal of pharmacology and chemotherapy 32,
295-310.
[21] Deradt, R., Jougney, S., Benzoni, J., Peterfalvi, M., (1980).Release of
prostaglandins E and F in an algogenic reaction and its inhibition
.European journal of pharmacology 61, 17-24.
[22] Martinez, V., Thakur, S., Mogil, J.S., Tache, Y., Mayer, E.A.,
1999.Differential effects of chemical and mechanical colonic irritation
on behavioral pain response to intraperitoneal acetic in mice. Pain 81,
163-185.
[23] Julius, D., Basbaum, A.I., (2001). Molecular mechanism of nociception
.Nature 413, 203-210.
[24] Hunskaar, S., Hole, K., (1987) .The formalin test in mice:dissociation
between inflammatory and non-inflammatory pain. Pain 30, 130-114.
[25] Tjolsen, A., Berge, O.G., Hunskaar, S., Roslan, J.H., Hole,K.,
(1992).The formain test: an evaluation of the method.Pain 51, 5-17.
[26] Crunkhorn P, Meacock S. C. R., (1971). Mediators of the inflammation
induced in the rat paw by carrageenan. Br J Pharmacol. 42(3): 392-402.
[27] Van Arman, C. G., Begany A. J., Miller, L. M. andPless, H. H. (2002).
Some details of the inflammations caused by yeast and carrageenin (with
appendix on kinetics of the reaction). J. Neurosci. 22:6208-6217.
[28] Ozaki, Y., (1990). Antiinflammatory effect of Curcuma xanthorrhiza
ROXB.and its active principles . Chemical and pharmaceutical
bulletin.1045-1048 (Tokyo).
[29] Babior,B.M, Kipnes,R.S, Curnutte, J.T. (1973). The production by
leucocytes of superoxide, a potential bactericidal agent. J Clin Invest,
52: 741-4.
[30] Sethi, S., Singh, M.P., Dikshit, M., (2000). Mechanism involved in the
augmentation of arachidonic acid-induced free radicalgeneration from
rat neutrophils following hypoxia-reoxygenation. Trom Res 98:445-50.
[31] Latenti, A., Ianaro, A., Moncada, S., Di Rosa, M.,(1992).Modulation of
acute inflammation by endogenous nitric oxide. Eur J Pharmacol
211:177-86.
[32] Di Rosa, M., Giroud, J.P., Willoughby, D.A., (1971).Studies of
mediators of the acute inflammatory response induced in rats indifferent
site by carrageenan and turpentine. J Pathol 104: 15-21.
[33] Abramson, S.B., Weissman, G., (1989). The mechanism of action of
nonsteroidal anti-inflammatory drugs. Arthritis Rheum 32: 1-9.
[34] McCord, J.M., (1974). Free radicals and inflammation: Protection of
synovial fluid by superoxidedismutase. Science 185: 529-31.
[35] Salvemini, D., Wang, Z.O., Bourdon, D.M., Stern, M.K., Currie, M.G.,
Manning, P.T.,(1996). Evidence of peroxynitrite involvement in the
carrageenan-induced rat pawoedema.Eur J pharmacol 303: 217-20.
[36] Cuzzocrea, S., Zingarelli, B., Gilard, E., Hake, P., Salzman, A.L., Szabo,
C.,(1998). Antiinflammatory effect of mercapoethylguanidine, a
combined inhibitor of nitricoxide synthase and peroxynitrite scavenger,
in carrageenan-induced models of inflammation .Free RadicBiolMed 24:
450-9.
[37] Al-Ghamdi, M.S.,(2001).The anti-inflammatory ,analgesic and
antipyretic activity of Nigella sativa .J Ethanopharmacol ,76:45-8 .
[38] Hess, S.M., Millonig, R.C., (1972) .Assay for anti-inflammatory drugs
.In: Lepow, I.H., Ward,P.A., (Eds.),Inflammation ,Mechanisms and
control. Publication of Academic Press, London.
[39] Menuka, A.,Thabrew, I., Lakshmi, A., Shiroma, H., (2010). Antiinflammatory
activity of Trichosanthescucumerina Linn.in rats .Journal
of Ethnopharmacology 131:538-543.
[40] Aitadafoun, M., Mounieri, C., Heyman, S.F., Binistic, C., Bon, C.,
Godfroid, J., (1996).4-Alkoxybenzamidines as new potent
phospholipase A2 inhibitors.Biochemical pharmacology 51, 737-742.
@article{"International Journal of Biological, Life and Agricultural Sciences:52527", author = "Aman Ahmed Alzubier and Patrick Nwabueze Okechukwu", title = "Investigation of Anti-Inflammatory, Antipyretic and Analgesic Effect of Yemeni Sidr Honey", abstract = "Traditionally, Yemini Sidr honey has been reported to
cure liver problems, stomach ulcers, and respiratory disorders. In this
experiment, we evaluated Yemeni Sidr honey for its ability to protect
inflammations caused by acetic acid and formalin -induced writhing,
carrageenan and histamine-induced paw oedema in experimental rat
model. Hyperpyrexia, membrane stabilizing activity, and
phytochemical screening of the honey was also examined. Yemini
Sidr Honey at (100, 200 and 500 mg/kg) exhibited a concentration
dependant inhibition of acetic acid induced and formalin induced
writhing, paw oedema induced by carrageenan & histamine, and
hyperpyrexia induced by brewer's yeast, it also inhibited membrane
stabilizing activity. Phytochemical screenings of the honey reveal the
presence of flavonoids, steroid, alkaloids, saponins and tannins. This
study suggested that Yemeni Sidr honey possess very strong antiinflammatory,
analgesic and antipyretic effects and these effects
would be a result of the phytochemicals present.", keywords = "Anti-inflammatory, Analgesic, Carrageenan, Aceticacid, Histamine, Yemini Sidr Honey", volume = "5", number = "8", pages = "431-6", }
