Induction of Hsp70 and Antioxidant Status in Porcine Granulosa Cells in Response to Deoxynivalenol and Zearalenone Exposure in vitro
The aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), total antioxidant status (TAS) and accumulation of Hsp70 in porcine ovarian granulosa cells after deoxynivalenol (DON) and zearalenone (ZEA) exposure in vitro. Porcine ovarian granulosa cells were incubated with DON/ZEA administrations as follows: group A (10/10 ng/mL), group B (100/100 ng/mL), group C (1000/1000 ng/mL), and the control group without any additions for 24h. In this study mycotoxins developed stress reaction of porcine ovarian granulosa cells and increased accumulation of Hsp70 what resulted in increasing activities of SOD and GPx in groups with lower doses of mycotoxins. High dose of DON and ZEA had opposite effect on GPx activity than the lower doses. Slight increase in TAS of porcine granulosa cells was observed after mycotoxins exposure. These results contribute towards the understanding of cellular stress and its response.
[1] R. Kumar, K. M. Ansari, B. P. Chaudhari. A. Dhawan, P. D. Dwidedi, S.K. Jain and M. Das, "Topical Application of Ochratoxin A Causes DNA Damage and Tumor Initiation in Mouse Skin”, Plos One, vol. 7(10), pp. e47280, 2012.
[2] J.F. Grove, "The Trichotecenes and Their Biosynthesis”, Fortschritte der ChemieorganisherNaturstoffe, vol. 88, pp. 63-130, 2007.
[3] J.G. Pace, M.R. Watts and W.J. Cantenbury, "T-2 Mycotoxin Inhibits Mitochondrial Protein Synthesis”, Toxicon, vol. 26, pp. 77-85, 1988.
[4] J.J. Pestka and A.T. Smolinski, "Deoxynivalenol: Toxicology and Potential Effects on Humans”, Journal of Toxicology and Environmental Health, part B, vol. 8(1), pp. 39-69, 2005.
[5] K. Suiyama, M. Muroi, K. Tanamoto, M. Nishijima and Y. Sugita-Konishi, "Deoxyvalenol and Nivalenol Inhibit Lipopolysaccharide-Induces Nitric Oxide Production by Mouse Macrophage Cells”, Toxicology Letters, vol. 192(2), pp. 150-154, 2010.
[6] E. Roda, T. Coccini, D. Acerbi, A.F. Castoldi and L. Manzo, "Comparative in vitro and ex-vivo Myelotoxicity of Aflatoxins B1 and M1 on Haematopoietic Progenitors (BFU-E, CFU-E, and CFU-GM): Species-related susceptibility”, Toxicology in Vitro, vol. 24, pp. 217-223, 2010.
[7] G. Ranzenigo, F. Caloni, F. Cremonesi, P.Y. Aad and L.J. Spicer, "Effects of Fusarium Mycotoxins on Steroid Production by Porcine Granulosa Cells”, Animal Reproduction Science, vol. 107(1-2), pp. 115-130, 2008.
[8] E.A. Chandler, D.R. Simpson, M.A. Thomsett and P. Nicholson, "Development of PCR Assays to Tri7 and Tri13 Trichothecene Biosynthetic Genes, and Characterisation of Chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis”, Physiological and Plant Pathology, vol. 62, pp. 355-367, 2003.
[9] D. Dinu, G.O. Bodea, C.D. Ceapa, M.C. Munteanu, F.I. Roming, A.I. Serban, A. Hermenean, M. Costache, O. Zarnescu and A. Dinischiotu, "Adapted Response of the Antioxidant Defense System to Oxidative Stress Induced by Deoxyvalenol in Hek-293 cells”, Toxicon, vol. 57(7-8), pp. 1023-1032, 2011.
[10] A. Zinedine, J.M. Soriano, J.C. Molto and J. Magnes, "Review on the Toxicity, Occurrence, Metabolism, Detoxifications and Intake of Zearalenone: An Estrogenic Mycotoxin”, Food and Chemical Toxicology, vol. 45, pp. 1-18, 2007.
[11] G.G. Kuiper, J.G. Lemmen, B. Carlsson, J.C. Corton, S.H. Safe, P.T. van der Saag, B. van der Burg and J.A. Gustafsson, "Interaction of Estrogenic Chemicals and Phytoestrogens with Estrogen Receptor Beta”, Endocrinology, vol. 139, pp. 4252-4263, 1998.
[12] M. Olsen, "Metabolism of zearalenone in farm animals”, Fusarium Mycotoxins, Taxonomy and Pathogenicity, 1st Ed.; Chekowski, J. Ed. Elsevier: Amsterdam-Oxford-New York, p.p. 167-177, 1989.
[13] M. Gajecki, "Zearalenone – Undesirable Substances in Feed”, Polish Journal of Veterinary Science, vol. 80, pp. 183-205, 2002.
[14] F. Minervini and M.E. Dell Aquila, "Zearalenone and Reproductive Function in Farm Animals”, International Journal of Molecular Sciences, vol. 9, pp. 2570-2584, 2008.
[15] S. Jiang, Z. Yang, W. Yang, J. Gao, F. Liu, C. Chen and F. Chi, "Physiopathological Effects of Zearalenone in Post-Weaning Female Piglets with or without Montmorillonite Clay Adsorbent”, Livestock Sciences, vol. 131, pp. 130-136, 2010.
[16] F. Caloni, G. Ranzenigo, F. Cremonesi and L.J. Spicer, "Effects of a Trichothecene, T-2 Toxin, on Proliferation and Steroid Production by Porcine Granulosa Cells”, Toxicon, vol. 54(3), pp. 337-344, 2009.
[17] H. Sies, "Oxidative Stress: From Basic Research to Clinical Application”, American Journal of Medicine, vol. 91, pp. 31S-38S, 1991.
[18] S.Y. Jiang, Y.B. Zang, W.R. Zang, J. Gao, F.X. Liu, J. Broomhead and F. Chi, "Effects of Purified Zearalenone on Growth Performance, Organ Size, Serum Metabolites, and Oxidative Stress in Postweaning Gilts”, Journal of Animal Science, vol. 89, pp. 3008-3015, 2011.
[19] N.N. Gessler, A.A. Averyanov and T.A. Belozerskaya, "Reactive Oxygen Species in Regulation of Fungal Development”, Biochemistry, vol. 72, pp. 1091-1109, 2007.
[20] M. Miskei, Z. Karanyi and I. Pocsi, "Annotation of Stress-Response Proteins in the Aspergilla”, Fungal Genetics and Biology, vol. 46(1), pp. S105-S120, 2009.
[21] L. Yin, J. Huang, W. Huang, D. Li, G. Wang and Y. Liu, "Microcystin-RR-Induced Accumulation of Reactive Oxygen Species and Alteration of Antioxidant Systems in Tobacco BY-2 cells”, Toxicon, vol. 46(5), pp. 507-512, 2005.
[22] V.S. Mary, M.G. Theumer, S.L. Arias and H.R. Rubinstein, "Reactive Oxygen Species Sources and Biomolecular Oxidative Damage Induced by Aflatoxin B1 and Fumonisin B1 in Rat Spleen Mononuclear Cells”, Toxicology, vol. 302(2-3), pp. 299-307, 2012.
[23] M. Akcam, R. Artan, A. Yilmaz, S. Ozdem, T. Gelen and M. Naziroglu, "Caffeic Acid Phenethyl Ester Modulates Aflatoxin B1-Induced hepatotoxIcity in Rats”, Cell Biochemistry and Function, in press, doi: 10.1002/cbf.2957. 2013
[24] M. Jaattela, "Heat Shock Proteins as Cellular Lifeguards”, Annual Medicine, vol. 31, pp. 261-271, 1999.
[25] A.V. Sirotkin and M. Bauer, Heat Shock Proteins in Porcine Ovary: Synthesis, Accumulation and Regulation by Stress and Hormones”, Cell Stress and Chaperons, vol. 16, pp. 379-387, 2011.
[26] C. Bouaziz, A. Bouslimi, R. Kadri, C. Zaied, H. Bacha and S. Abid-Essefi, "The in vitro Effects of Zearalenone and T-2 Toxins on Vero Cells”, Experimental and Toxicologic Pathology, vol. 65(5), pp. 497-501, 2013.
[27] M. Medvedova, A. Kolesarova, M. Capcarova, R. Labuda, A.V. Sirotkin, J. Kovacik and J. Bulla, "The Effect of Deoxynivalenol on the Secretion Activity, Proliferation and Apoptosis of Porcine Ovarian Granulosa Cells in vitro”, Journal of Environmental Science and Health, Part B, vol. 46, pp. 213-219, 2011.
[28] A. Kolesarova, M. Capcarova, N. Maruniakova, N. Lukac, R.E. Ciereszko and A.V. Sirotkin, "Resveratrol Inhibits Reproductive Toxicity Induced by Deoxyvalenol”, Journal of Environmental Science and Health, Part A, vol. 47, pp. 1329-1334, 2012.
[29] A. Kolesarova, M. Capcarova, A. Sirotkin, M. Medvedova and J. Kovacik, "Cobalt-Induced Changes the IGF-I and Progesteron Release, Expression of Proliferation- and Apoptosis-Related Peptides in Porcine Ovarian Granulosa Cells in vitro”, Journal of Environmental Science and Health, Part A, vol. 42(7), pp. 810-817, 2010.
[30] A.V. Sirotkin and A.V. Makarevich, „GH Regulates Secretory Activity and Apoptosis in Cultured Bovine Granulosa Cells through the Activation of the cAMP/Protein Dinase A System”, Journal of Endocrinology, vol. 163, p. 317–327, 1999.
[31] U. Tiemann, W. Tomek, F. Schneider, M. Müller, R. Pöhland and J. Vanselow, "The Mycotoxinsalternariol and Alternariol Methyl Ether Negatively Affect Progesterone Synthesis in Porcine Granulosa Cells in vitro”, Toxicology Letters, vol. 186(2), pp. 139-145, 2009.
[32] L. Zhu, H. Yuan, C. Guo, Y. Lu, Y. Deng, Q. Wei, L. Wen and Z. He, "Zearalenone Induces Apoptosis and Necrosis in Porcine Granulosa Cells via a Caspase-3 and Caspase-9-Dependent Mitochondrial Signalling Pathway”, Journal of Cell Physiology, vol. 227(5), pp. 1814-1820, 2012.
[33] J. Wu, L. Jing, H. Yuan and S.Q. Peng, "T-2 Toxin Induces Apoptosis in Ovarian Granulosa Cells of Rats through Reactive Oxygen Species-Mediated Mitochondrial Pathway”, Toxicology Letters, vol. 202(3), pp. 168-177, 2011.
[34] R. Borutova, S. Faix, I. Placha, L. Gresakova, K. Cobanova and L. Leng, "Effects of Deoxynivalenol and Zearalenone on Oxidative Stress and Blood Phagocytic Activity in Broilers”, Archives of Animal Nutrition, vol. 62, pp. 303-312, 2008.
[35] X. Zhang, L. Jiang, C. Geng, J. Cao and L. Zhong, "The role of Oxidative Stress in Deoxynivalenol-Induced DNA Damage in HepG2 Cells”, Toxicon, vol. 54, pp. 513-518, 2009.
[36] S.P. Boeira, C.B. Filho, L. DelFabbro, L.F. Royes, C.R. Jessé, M.S. Oliveira and A.F. Furian, "Possible Role for Glutathione-S-Transferase in the Oligozoospermia Elicited by Acute Zearalenone Administration in Swiss Albino Mice”, Toxicon, vol. 60(3), pp. 358-366, 2012.
[37] M. Chaudhari, R. Jayaraj, S.R. Santhosh and P.V. Rao, "Oxidative Damage and Gene Expression Profile of Antioxidant Enzymes after T-2 Toxin Exposure in Mice”, Journal of Biochemical and Molecular Toxicology, vol. 23(3), pp. 212-221, 2009.
[38] I. Placha, R. Borutova, I. Gresakova, V. Petrovic, S. Faix and L. Leng, "Effect of Excessive Selenium Supplementation to Diet Contaminated with Deoxynivalenol on Blood Phagocytic Activity and Antioxidant Status of Broilers”, Journal of Animal Physiology and Animal Nutrition, vol. 93, pp. 695-702, 2009.
[39] D.E. Marin, G.C. Pistol, I.V. Neagoe, L. Calin and I. Taranu, "Effects of Zearalenone on Oxidative Stress and Inflammation in Weanling Piglets”, Food and Chemical Toxicology, vol. 58, pp. 408-415, 2013.
[40] J. Wu, D. Tu, L.Y. Yuan and L.X. Wen, "T-2 Toxin Exposure Induces Apoptosis in Rat Ovarian Granulosa Cells through Oxidative Stress”, Environmental Toxicology and Pharmacology, vol. 36, pp. 493-500, 2013.
[41] Y.J. Hou, Y.Y. Zhao, B. Xiong, X.S. Cui, N.H. Kim, Y.X. Xu and S.S. Sun, "Mycotoxin-Containing Diet Causes Oxidative Stress in the Mouse”, PloS One, vol. 8, pp. e60374, 2013.
[42] O. Rocha, K. Ansari and F.M. Doohan, "Effects of Trichothecenemycotoxins on Eukaryotic Cells: A Review”, Food Additives and Contaminants, vol. 22(4), pp. 369-378, 2005.
[43] F. Gao, L.P. Jiang, M. Chen, C.Y. Geng, G. Yang, F. Ji, L.F. Zhong, and X.F. Liu, "Genotoxic Effects Induced by Zearalenone in a Human Embryonic Kidney Cell Line”, Mutation Research, vol. 755, pp. 6-10, 2013.
[44] . El GolliBennour, C. Bouaziz, M. Ladjimi, F. Renaud, and H. Bacha, "Comparative Mechanism of Zearalenone and Ochratoxin A Toxicities on Cultured HepG2 Cells: Is Oxidative Stress a Common Process?”, Environmental Toxicology, vol. 24(6), pp. 538-548, 2009.
[45] A. De Maio, „Extracellular Heat Shock Proteins, Cellular Export Vesicles, and the Stress Observation System: A Form of Communication during Injury, Infection, and Cell Damage", Cell Stress Chaperones, vol. 16(3), pp. 235-249, 2011.
[46] B. McLaughlin, K.A. Hartnett, J.A. Erhardt, J.J. Legos, R.F. White, F.C. Barone and E. Aizenman, „Caspase 3 Activation is Essential for Neuroprotection in Preconditioning", Proceedings of the National Academy of Sciences of USA, vol. 100(2), pp. 715-720, 2003.
[47] S. Guo, W. Wharton, P. Moseley and H. Shi, „Heat Shock Protein 70 Regulates Cellular Redox Status by Modulating Glutathione-Related enzyme activities”, Cell Stress and Chaperones, vol. 12, p. 245-254, 2007.
[48] M. Capcarova, A. Kolesarova and A.V. Sirotkin, "Antioxidant Status and Expression of Heat Shock Protein of Cobalt-Treated Porcine Ovarian Granulosa Cells”, Journal of Microbiology, Biotechnology and Food Sciences, vol. 2(1), pp. 11715-11720, 2013.
[1] R. Kumar, K. M. Ansari, B. P. Chaudhari. A. Dhawan, P. D. Dwidedi, S.K. Jain and M. Das, "Topical Application of Ochratoxin A Causes DNA Damage and Tumor Initiation in Mouse Skin”, Plos One, vol. 7(10), pp. e47280, 2012.
[2] J.F. Grove, "The Trichotecenes and Their Biosynthesis”, Fortschritte der ChemieorganisherNaturstoffe, vol. 88, pp. 63-130, 2007.
[3] J.G. Pace, M.R. Watts and W.J. Cantenbury, "T-2 Mycotoxin Inhibits Mitochondrial Protein Synthesis”, Toxicon, vol. 26, pp. 77-85, 1988.
[4] J.J. Pestka and A.T. Smolinski, "Deoxynivalenol: Toxicology and Potential Effects on Humans”, Journal of Toxicology and Environmental Health, part B, vol. 8(1), pp. 39-69, 2005.
[5] K. Suiyama, M. Muroi, K. Tanamoto, M. Nishijima and Y. Sugita-Konishi, "Deoxyvalenol and Nivalenol Inhibit Lipopolysaccharide-Induces Nitric Oxide Production by Mouse Macrophage Cells”, Toxicology Letters, vol. 192(2), pp. 150-154, 2010.
[6] E. Roda, T. Coccini, D. Acerbi, A.F. Castoldi and L. Manzo, "Comparative in vitro and ex-vivo Myelotoxicity of Aflatoxins B1 and M1 on Haematopoietic Progenitors (BFU-E, CFU-E, and CFU-GM): Species-related susceptibility”, Toxicology in Vitro, vol. 24, pp. 217-223, 2010.
[7] G. Ranzenigo, F. Caloni, F. Cremonesi, P.Y. Aad and L.J. Spicer, "Effects of Fusarium Mycotoxins on Steroid Production by Porcine Granulosa Cells”, Animal Reproduction Science, vol. 107(1-2), pp. 115-130, 2008.
[8] E.A. Chandler, D.R. Simpson, M.A. Thomsett and P. Nicholson, "Development of PCR Assays to Tri7 and Tri13 Trichothecene Biosynthetic Genes, and Characterisation of Chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis”, Physiological and Plant Pathology, vol. 62, pp. 355-367, 2003.
[9] D. Dinu, G.O. Bodea, C.D. Ceapa, M.C. Munteanu, F.I. Roming, A.I. Serban, A. Hermenean, M. Costache, O. Zarnescu and A. Dinischiotu, "Adapted Response of the Antioxidant Defense System to Oxidative Stress Induced by Deoxyvalenol in Hek-293 cells”, Toxicon, vol. 57(7-8), pp. 1023-1032, 2011.
[10] A. Zinedine, J.M. Soriano, J.C. Molto and J. Magnes, "Review on the Toxicity, Occurrence, Metabolism, Detoxifications and Intake of Zearalenone: An Estrogenic Mycotoxin”, Food and Chemical Toxicology, vol. 45, pp. 1-18, 2007.
[11] G.G. Kuiper, J.G. Lemmen, B. Carlsson, J.C. Corton, S.H. Safe, P.T. van der Saag, B. van der Burg and J.A. Gustafsson, "Interaction of Estrogenic Chemicals and Phytoestrogens with Estrogen Receptor Beta”, Endocrinology, vol. 139, pp. 4252-4263, 1998.
[12] M. Olsen, "Metabolism of zearalenone in farm animals”, Fusarium Mycotoxins, Taxonomy and Pathogenicity, 1st Ed.; Chekowski, J. Ed. Elsevier: Amsterdam-Oxford-New York, p.p. 167-177, 1989.
[13] M. Gajecki, "Zearalenone – Undesirable Substances in Feed”, Polish Journal of Veterinary Science, vol. 80, pp. 183-205, 2002.
[14] F. Minervini and M.E. Dell Aquila, "Zearalenone and Reproductive Function in Farm Animals”, International Journal of Molecular Sciences, vol. 9, pp. 2570-2584, 2008.
[15] S. Jiang, Z. Yang, W. Yang, J. Gao, F. Liu, C. Chen and F. Chi, "Physiopathological Effects of Zearalenone in Post-Weaning Female Piglets with or without Montmorillonite Clay Adsorbent”, Livestock Sciences, vol. 131, pp. 130-136, 2010.
[16] F. Caloni, G. Ranzenigo, F. Cremonesi and L.J. Spicer, "Effects of a Trichothecene, T-2 Toxin, on Proliferation and Steroid Production by Porcine Granulosa Cells”, Toxicon, vol. 54(3), pp. 337-344, 2009.
[17] H. Sies, "Oxidative Stress: From Basic Research to Clinical Application”, American Journal of Medicine, vol. 91, pp. 31S-38S, 1991.
[18] S.Y. Jiang, Y.B. Zang, W.R. Zang, J. Gao, F.X. Liu, J. Broomhead and F. Chi, "Effects of Purified Zearalenone on Growth Performance, Organ Size, Serum Metabolites, and Oxidative Stress in Postweaning Gilts”, Journal of Animal Science, vol. 89, pp. 3008-3015, 2011.
[19] N.N. Gessler, A.A. Averyanov and T.A. Belozerskaya, "Reactive Oxygen Species in Regulation of Fungal Development”, Biochemistry, vol. 72, pp. 1091-1109, 2007.
[20] M. Miskei, Z. Karanyi and I. Pocsi, "Annotation of Stress-Response Proteins in the Aspergilla”, Fungal Genetics and Biology, vol. 46(1), pp. S105-S120, 2009.
[21] L. Yin, J. Huang, W. Huang, D. Li, G. Wang and Y. Liu, "Microcystin-RR-Induced Accumulation of Reactive Oxygen Species and Alteration of Antioxidant Systems in Tobacco BY-2 cells”, Toxicon, vol. 46(5), pp. 507-512, 2005.
[22] V.S. Mary, M.G. Theumer, S.L. Arias and H.R. Rubinstein, "Reactive Oxygen Species Sources and Biomolecular Oxidative Damage Induced by Aflatoxin B1 and Fumonisin B1 in Rat Spleen Mononuclear Cells”, Toxicology, vol. 302(2-3), pp. 299-307, 2012.
[23] M. Akcam, R. Artan, A. Yilmaz, S. Ozdem, T. Gelen and M. Naziroglu, "Caffeic Acid Phenethyl Ester Modulates Aflatoxin B1-Induced hepatotoxIcity in Rats”, Cell Biochemistry and Function, in press, doi: 10.1002/cbf.2957. 2013
[24] M. Jaattela, "Heat Shock Proteins as Cellular Lifeguards”, Annual Medicine, vol. 31, pp. 261-271, 1999.
[25] A.V. Sirotkin and M. Bauer, Heat Shock Proteins in Porcine Ovary: Synthesis, Accumulation and Regulation by Stress and Hormones”, Cell Stress and Chaperons, vol. 16, pp. 379-387, 2011.
[26] C. Bouaziz, A. Bouslimi, R. Kadri, C. Zaied, H. Bacha and S. Abid-Essefi, "The in vitro Effects of Zearalenone and T-2 Toxins on Vero Cells”, Experimental and Toxicologic Pathology, vol. 65(5), pp. 497-501, 2013.
[27] M. Medvedova, A. Kolesarova, M. Capcarova, R. Labuda, A.V. Sirotkin, J. Kovacik and J. Bulla, "The Effect of Deoxynivalenol on the Secretion Activity, Proliferation and Apoptosis of Porcine Ovarian Granulosa Cells in vitro”, Journal of Environmental Science and Health, Part B, vol. 46, pp. 213-219, 2011.
[28] A. Kolesarova, M. Capcarova, N. Maruniakova, N. Lukac, R.E. Ciereszko and A.V. Sirotkin, "Resveratrol Inhibits Reproductive Toxicity Induced by Deoxyvalenol”, Journal of Environmental Science and Health, Part A, vol. 47, pp. 1329-1334, 2012.
[29] A. Kolesarova, M. Capcarova, A. Sirotkin, M. Medvedova and J. Kovacik, "Cobalt-Induced Changes the IGF-I and Progesteron Release, Expression of Proliferation- and Apoptosis-Related Peptides in Porcine Ovarian Granulosa Cells in vitro”, Journal of Environmental Science and Health, Part A, vol. 42(7), pp. 810-817, 2010.
[30] A.V. Sirotkin and A.V. Makarevich, „GH Regulates Secretory Activity and Apoptosis in Cultured Bovine Granulosa Cells through the Activation of the cAMP/Protein Dinase A System”, Journal of Endocrinology, vol. 163, p. 317–327, 1999.
[31] U. Tiemann, W. Tomek, F. Schneider, M. Müller, R. Pöhland and J. Vanselow, "The Mycotoxinsalternariol and Alternariol Methyl Ether Negatively Affect Progesterone Synthesis in Porcine Granulosa Cells in vitro”, Toxicology Letters, vol. 186(2), pp. 139-145, 2009.
[32] L. Zhu, H. Yuan, C. Guo, Y. Lu, Y. Deng, Q. Wei, L. Wen and Z. He, "Zearalenone Induces Apoptosis and Necrosis in Porcine Granulosa Cells via a Caspase-3 and Caspase-9-Dependent Mitochondrial Signalling Pathway”, Journal of Cell Physiology, vol. 227(5), pp. 1814-1820, 2012.
[33] J. Wu, L. Jing, H. Yuan and S.Q. Peng, "T-2 Toxin Induces Apoptosis in Ovarian Granulosa Cells of Rats through Reactive Oxygen Species-Mediated Mitochondrial Pathway”, Toxicology Letters, vol. 202(3), pp. 168-177, 2011.
[34] R. Borutova, S. Faix, I. Placha, L. Gresakova, K. Cobanova and L. Leng, "Effects of Deoxynivalenol and Zearalenone on Oxidative Stress and Blood Phagocytic Activity in Broilers”, Archives of Animal Nutrition, vol. 62, pp. 303-312, 2008.
[35] X. Zhang, L. Jiang, C. Geng, J. Cao and L. Zhong, "The role of Oxidative Stress in Deoxynivalenol-Induced DNA Damage in HepG2 Cells”, Toxicon, vol. 54, pp. 513-518, 2009.
[36] S.P. Boeira, C.B. Filho, L. DelFabbro, L.F. Royes, C.R. Jessé, M.S. Oliveira and A.F. Furian, "Possible Role for Glutathione-S-Transferase in the Oligozoospermia Elicited by Acute Zearalenone Administration in Swiss Albino Mice”, Toxicon, vol. 60(3), pp. 358-366, 2012.
[37] M. Chaudhari, R. Jayaraj, S.R. Santhosh and P.V. Rao, "Oxidative Damage and Gene Expression Profile of Antioxidant Enzymes after T-2 Toxin Exposure in Mice”, Journal of Biochemical and Molecular Toxicology, vol. 23(3), pp. 212-221, 2009.
[38] I. Placha, R. Borutova, I. Gresakova, V. Petrovic, S. Faix and L. Leng, "Effect of Excessive Selenium Supplementation to Diet Contaminated with Deoxynivalenol on Blood Phagocytic Activity and Antioxidant Status of Broilers”, Journal of Animal Physiology and Animal Nutrition, vol. 93, pp. 695-702, 2009.
[39] D.E. Marin, G.C. Pistol, I.V. Neagoe, L. Calin and I. Taranu, "Effects of Zearalenone on Oxidative Stress and Inflammation in Weanling Piglets”, Food and Chemical Toxicology, vol. 58, pp. 408-415, 2013.
[40] J. Wu, D. Tu, L.Y. Yuan and L.X. Wen, "T-2 Toxin Exposure Induces Apoptosis in Rat Ovarian Granulosa Cells through Oxidative Stress”, Environmental Toxicology and Pharmacology, vol. 36, pp. 493-500, 2013.
[41] Y.J. Hou, Y.Y. Zhao, B. Xiong, X.S. Cui, N.H. Kim, Y.X. Xu and S.S. Sun, "Mycotoxin-Containing Diet Causes Oxidative Stress in the Mouse”, PloS One, vol. 8, pp. e60374, 2013.
[42] O. Rocha, K. Ansari and F.M. Doohan, "Effects of Trichothecenemycotoxins on Eukaryotic Cells: A Review”, Food Additives and Contaminants, vol. 22(4), pp. 369-378, 2005.
[43] F. Gao, L.P. Jiang, M. Chen, C.Y. Geng, G. Yang, F. Ji, L.F. Zhong, and X.F. Liu, "Genotoxic Effects Induced by Zearalenone in a Human Embryonic Kidney Cell Line”, Mutation Research, vol. 755, pp. 6-10, 2013.
[44] . El GolliBennour, C. Bouaziz, M. Ladjimi, F. Renaud, and H. Bacha, "Comparative Mechanism of Zearalenone and Ochratoxin A Toxicities on Cultured HepG2 Cells: Is Oxidative Stress a Common Process?”, Environmental Toxicology, vol. 24(6), pp. 538-548, 2009.
[45] A. De Maio, „Extracellular Heat Shock Proteins, Cellular Export Vesicles, and the Stress Observation System: A Form of Communication during Injury, Infection, and Cell Damage", Cell Stress Chaperones, vol. 16(3), pp. 235-249, 2011.
[46] B. McLaughlin, K.A. Hartnett, J.A. Erhardt, J.J. Legos, R.F. White, F.C. Barone and E. Aizenman, „Caspase 3 Activation is Essential for Neuroprotection in Preconditioning", Proceedings of the National Academy of Sciences of USA, vol. 100(2), pp. 715-720, 2003.
[47] S. Guo, W. Wharton, P. Moseley and H. Shi, „Heat Shock Protein 70 Regulates Cellular Redox Status by Modulating Glutathione-Related enzyme activities”, Cell Stress and Chaperones, vol. 12, p. 245-254, 2007.
[48] M. Capcarova, A. Kolesarova and A.V. Sirotkin, "Antioxidant Status and Expression of Heat Shock Protein of Cobalt-Treated Porcine Ovarian Granulosa Cells”, Journal of Microbiology, Biotechnology and Food Sciences, vol. 2(1), pp. 11715-11720, 2013.
@article{"International Journal of Biological, Life and Agricultural Sciences:66779", author = "Marcela Capcarova and Adriana Kolesarova and Marina Medvedova and Peter Petruska and Alexander V. Sirotkin", title = "Induction of Hsp70 and Antioxidant Status in Porcine Granulosa Cells in Response to Deoxynivalenol and Zearalenone Exposure in vitro", abstract = "The aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), total antioxidant status (TAS) and accumulation of Hsp70 in porcine ovarian granulosa cells after deoxynivalenol (DON) and zearalenone (ZEA) exposure in vitro. Porcine ovarian granulosa cells were incubated with DON/ZEA administrations as follows: group A (10/10 ng/mL), group B (100/100 ng/mL), group C (1000/1000 ng/mL), and the control group without any additions for 24h. In this study mycotoxins developed stress reaction of porcine ovarian granulosa cells and increased accumulation of Hsp70 what resulted in increasing activities of SOD and GPx in groups with lower doses of mycotoxins. High dose of DON and ZEA had opposite effect on GPx activity than the lower doses. Slight increase in TAS of porcine granulosa cells was observed after mycotoxins exposure. These results contribute towards the understanding of cellular stress and its response.
", keywords = "Deoxynivalenol, zearalenone, antioxidants, Hsp70, granulosa cells.", volume = "8", number = "3", pages = "248-6", }
