Human Elastin-derived Biomimetic Coating Surface to Support Cell Growth
A new sythetic gene coding for a Human
Elastin-Like Polypeptide was constructed and expressed. The
recombinant product was tested as coating agent to realize a
surface suitable for cell growth. Coatings showed peculiar
features and different human cell lines were seeded and
cultured. All cell lines tested showed to adhere and proliferate
on this substrate that has been shown also to exert a specific
effect on cells, depending on cell type.
[1] J.Y. Lim and H.J. Donahue "Cell sensing and response to micro- and
nanostructured surfaces produced by chemical and topographic
patterning," Tissue Eng., vol. 13, pp. 1879-1891, Aug. 2007.
[2] P. Roach, D. Eglin, K. Rohde and C.C. Perry "Modern biomaterials: a
review - bulk properties and implications of surface modifications," J.
Mater. Sci. Mater. Med., vol. 18, pp. 1263-1277, July 2007.
[3] M.S. Bretscher "On the shape of migrating cells-a 'front-to-back' model,"
J. Cell Sci., vol.121, pp. 2625-2628, Aug. 2008
[4] M.P. Lutolf and J.A. Hubbell "Synthetic biomaterials as instructive
extracellular microenvironments for morphogenesis in tissue
engineering," Nat. Biotechnol.,vol. 23, pp. 47-55, Jan. 2005.
[5] V.A. Schulte, M. D├¡ez, M. Möller and M.C. Lensen "Surface topography
induces fibroblast adhesion on intrinsically nonadhesive poly(ethylene
glycol) substrates," Biomacromolecules, vol. 10, pp. 2795-2801, Oct.
2009.
[6] K.S. Straley and S.C. Heilshorn "Design and adsorption of modular
engineered proteins to prepare customized, neuron-compatible coatings,"
Front. Neuroengineerin., vol. 2., pp. -10, Jun. 2009.
[7] V. Hasirci and H. Kenar "Novel surface patterning approaches for tissue
engineering and their effect on cell behavior," Nanomed., vol. 1, pp. 73-
90. Jun. 2006.
[8] Y. Ikada "Challenges in tissue engineering," J. R. Soc. Interface, vol. 3,
pp. 589-601, Oct. 2006.
[9] W.L. Grayson, T.P. Martens, G.M. Eng, M. Radisic and G. Vunjak-
Novakovic "Biomimetic approach to tissue engineering," Semin Cell
Dev. Biol., vol. 20, 665-673, Aug. 2009.
[10] A. Chilkoti, T. Christensen and J.A. MacKay "Stimulus responsive
elastin biopolymers: Applications in medicine and biotechnology," Curr.
Opin. Chem. Biol., vol. 10, pp. 652-657, Dec. 2006.
[11] D.W. Urry " Characterization of soluble peptides of elastin by physical
techniques," Methods Enzymol., vol. 82, pt A, pp. 673-716, 1982.
[12] D.W. Urry, T. Hugel, M. Seitz, H.E. Gaub, L. Sheiba, J. Dea, J. Xu and
T. Parker. "Elastin: a representative ideal protein elastomer," Philos.
Trans. R. Soc. Lond. B Biol. Sci., vol. 357, pp.169-184, Feb. 2002.
[13] A. Chilkoti, M.R. Dreher and D.E. Meyer., "Design of thermally
responsive, recombinant polypeptide carriers for targeted drug delivery,"
Adv. Drug Deliv. Rev., vol. 54, pp. 1093-1111, Oct. 2002.
[14] A. Nagarsekar, J. Crissman, M. Crissman, F. Ferrari, J. Cappello and H.
Ghandehari "Genetic engineering of stimuli-sensitive silkelastin-like
protein block copolymers," Biomacromolecules, vol. 4, pp. 602-607,
May-Jun 2003.
[15] J.C. Rodríguez-Cabello, S. Prieto, J. Reguera, F.J. Arias and A. Ribeiro,
"Biofunctional design of elastin-like polymers for advanced applications
in nanobiotechnology," J. Biomater. Sci. Polym. Ed., vol. 18, pp. 269-
286, 2007.
[16] C.M. Bellingham, K.A. Woodhouse, P. Robson, S.J. Rothstein and FW
Keeley, "Self-aggregation characteristics of recombinantly expressed
human elastin polypeptides," Biochim. Biophys. Acta, vol. 1550, pp. 6-
19, Nov 2001.
[17] A. Bandiera, A. Taglienti, F. Micali, B. Pani, M. Tamaro, V. Crescenzi
and G. Manzini "Expression and characterization of human-elastinrepeat-
based temperature-responsive protein polymers for
biotechnological purposes," Biotechnol. Appl. Biochem., vol. 42, pp.
247-256, Dec 2005.
[18] A. Bandiera "Assembly and optimization of expression of synthetic
genes derived from the human elastin repeated motif," Prep. Biochem.
Biotechnol., to be published.
[19] R. Langer and D.A. Tirrell "Designing materials for biology and
medicine," Nature, vol. 428, pp. 487-492, Apr. 2004.
[20] D. Chow, M.L. Nunalee, D.W. Lim, A.J. Simnick and A. Chilkoti
"Peptide-based Biopolymers in Biomedicine and Biotechnology," Mater.
Sci. Eng. R. Rep., vol. 62, pp. 125-155, Jan 2008.
[1] J.Y. Lim and H.J. Donahue "Cell sensing and response to micro- and
nanostructured surfaces produced by chemical and topographic
patterning," Tissue Eng., vol. 13, pp. 1879-1891, Aug. 2007.
[2] P. Roach, D. Eglin, K. Rohde and C.C. Perry "Modern biomaterials: a
review - bulk properties and implications of surface modifications," J.
Mater. Sci. Mater. Med., vol. 18, pp. 1263-1277, July 2007.
[3] M.S. Bretscher "On the shape of migrating cells-a 'front-to-back' model,"
J. Cell Sci., vol.121, pp. 2625-2628, Aug. 2008
[4] M.P. Lutolf and J.A. Hubbell "Synthetic biomaterials as instructive
extracellular microenvironments for morphogenesis in tissue
engineering," Nat. Biotechnol.,vol. 23, pp. 47-55, Jan. 2005.
[5] V.A. Schulte, M. D├¡ez, M. Möller and M.C. Lensen "Surface topography
induces fibroblast adhesion on intrinsically nonadhesive poly(ethylene
glycol) substrates," Biomacromolecules, vol. 10, pp. 2795-2801, Oct.
2009.
[6] K.S. Straley and S.C. Heilshorn "Design and adsorption of modular
engineered proteins to prepare customized, neuron-compatible coatings,"
Front. Neuroengineerin., vol. 2., pp. -10, Jun. 2009.
[7] V. Hasirci and H. Kenar "Novel surface patterning approaches for tissue
engineering and their effect on cell behavior," Nanomed., vol. 1, pp. 73-
90. Jun. 2006.
[8] Y. Ikada "Challenges in tissue engineering," J. R. Soc. Interface, vol. 3,
pp. 589-601, Oct. 2006.
[9] W.L. Grayson, T.P. Martens, G.M. Eng, M. Radisic and G. Vunjak-
Novakovic "Biomimetic approach to tissue engineering," Semin Cell
Dev. Biol., vol. 20, 665-673, Aug. 2009.
[10] A. Chilkoti, T. Christensen and J.A. MacKay "Stimulus responsive
elastin biopolymers: Applications in medicine and biotechnology," Curr.
Opin. Chem. Biol., vol. 10, pp. 652-657, Dec. 2006.
[11] D.W. Urry " Characterization of soluble peptides of elastin by physical
techniques," Methods Enzymol., vol. 82, pt A, pp. 673-716, 1982.
[12] D.W. Urry, T. Hugel, M. Seitz, H.E. Gaub, L. Sheiba, J. Dea, J. Xu and
T. Parker. "Elastin: a representative ideal protein elastomer," Philos.
Trans. R. Soc. Lond. B Biol. Sci., vol. 357, pp.169-184, Feb. 2002.
[13] A. Chilkoti, M.R. Dreher and D.E. Meyer., "Design of thermally
responsive, recombinant polypeptide carriers for targeted drug delivery,"
Adv. Drug Deliv. Rev., vol. 54, pp. 1093-1111, Oct. 2002.
[14] A. Nagarsekar, J. Crissman, M. Crissman, F. Ferrari, J. Cappello and H.
Ghandehari "Genetic engineering of stimuli-sensitive silkelastin-like
protein block copolymers," Biomacromolecules, vol. 4, pp. 602-607,
May-Jun 2003.
[15] J.C. Rodríguez-Cabello, S. Prieto, J. Reguera, F.J. Arias and A. Ribeiro,
"Biofunctional design of elastin-like polymers for advanced applications
in nanobiotechnology," J. Biomater. Sci. Polym. Ed., vol. 18, pp. 269-
286, 2007.
[16] C.M. Bellingham, K.A. Woodhouse, P. Robson, S.J. Rothstein and FW
Keeley, "Self-aggregation characteristics of recombinantly expressed
human elastin polypeptides," Biochim. Biophys. Acta, vol. 1550, pp. 6-
19, Nov 2001.
[17] A. Bandiera, A. Taglienti, F. Micali, B. Pani, M. Tamaro, V. Crescenzi
and G. Manzini "Expression and characterization of human-elastinrepeat-
based temperature-responsive protein polymers for
biotechnological purposes," Biotechnol. Appl. Biochem., vol. 42, pp.
247-256, Dec 2005.
[18] A. Bandiera "Assembly and optimization of expression of synthetic
genes derived from the human elastin repeated motif," Prep. Biochem.
Biotechnol., to be published.
[19] R. Langer and D.A. Tirrell "Designing materials for biology and
medicine," Nature, vol. 428, pp. 487-492, Apr. 2004.
[20] D. Chow, M.L. Nunalee, D.W. Lim, A.J. Simnick and A. Chilkoti
"Peptide-based Biopolymers in Biomedicine and Biotechnology," Mater.
Sci. Eng. R. Rep., vol. 62, pp. 125-155, Jan 2008.
@article{"International Journal of Biological, Life and Agricultural Sciences:64659", author = "Antonella Bandiera", title = "Human Elastin-derived Biomimetic Coating Surface to Support Cell Growth", abstract = "A new sythetic gene coding for a Human
Elastin-Like Polypeptide was constructed and expressed. The
recombinant product was tested as coating agent to realize a
surface suitable for cell growth. Coatings showed peculiar
features and different human cell lines were seeded and
cultured. All cell lines tested showed to adhere and proliferate
on this substrate that has been shown also to exert a specific
effect on cells, depending on cell type.", keywords = "elastin, recombinant protein, coating, cell adhesion.", volume = "4", number = "1", pages = "137-5", }
