HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers
Protorhus longifolia is known as a medicinal plant that has been used traditionally to treat various ailments such as hemiplegic paralysis, blood clotting related diseases, diarrhoea, heartburn, etc. The study reports a High-Performance Thin Layer Chromatography (HPTLC) fingerprint profile of Protorhus longifolia methanolic extract and its qualitative analysis of gallic acid, rutin, and quercetin. HPTLC analysis was achieved using CAMAG HPTLC system equipped with CAMAG automatic TLC sampler 4, CAMAG Automatic Developing Chamber 2 (ADC2), CAMAG visualizer 2, CAMAG Thin Layer Chromatography (TLC) scanner and visionCATS CAMAG HPTLC software. Mobile phase comprising toluene, ethyl acetate, formic acid (21:15:3) was used for qualitative analysis of gallic acid and revealed eight peaks while the mobile phase containing ethyl acetate, water, glacial acetic acid, formic acid (100:26:11:11) for qualitative analysis of rutin and quercetin revealed six peaks. HPTLC sillica gel 60 F254 glass plates (10 × 10) were used as the stationary phase. Gallic acid was detected at the Rf = 0.35; while rutin and quercetin were not evident in the extract. Further studies will be performed to quantify gallic acid in Protorhus longifolia leaves and also identify other biomarkers.
[1] R. A. Street and G. Prinsloo, “Commercially important medicinal plants of South Africa”, J. Chem. vol. 2013, pp. 1-16, November 2012.
[2] M. Saxena, J. Saxena, R Nema, D. Singh and A. Gupta “Phytochemistry of medicinal plants”, J Pharmacogn Phytochem. vol. 1, pp. 168-182, March 2013.
[3] Y. A. Barku, Wound Healing-Current Perspectives. IntechOpen, 2019, ch.5.
[4] M. F. Mahomoodally, “Traditional medicines in Africa: An appraisal of ten potent African medicinal plants”, Evid Based Complement Alternat Med. vol. 2013, pp. 1-14, October 2013.
[5] S. Van Vuuren and D. Holl, "Antimicrobial natural product research: a review from a South African perspective for the years 2009–2016”, J. Ethnopharmacol. vol. 208, pp. 236-252, August 2017.
[6] L. V. Buwa-Komoren, B. Mayekiso, Z. Mhinana, and A. L. Adeniran, “An ethnobotanical and ethnomedicinal survey of traditionally used medicinal plants in Seymour, South Africa: An attempt toward digitization and preservation of ethnic knowledge”, Pharmacogn. Mag. vol. 15, pp. 115-123, January 2019.
[7] S. H. Nile and Park, S. W, “HPTLC analysis, antioxidant and antigout activity of Indian plants. Iran J Pharm Res. vol. 13, pp. 531-539, September 2014.
[8] L. Seasotiya, P. Siwach, A. Malik, S. Bai, P. Bharti and S. Dalal, “ Phytochemical evaluation and HPTLC fingerprint profile of Cassia fistula”, Int. J. Adv. Pharm., Biol. Chem. vol. 3, pp. 604-611, September 2014.
[9] A. K. Chandrakar, “Phytochemical fingerprinting through computerized HPTLC System for quality control of herbal drugs”, nt. j. res. appl. sci. eng. technol. vol. 6, pp. 733-738, January 2019.
[10] D. Gomathi, G. Ravikumar, M. Kalaiselvi, B. Vidya and C. Uma, “HPTLC fingerprinting analysis of Evolvulus alsinoides (L.) L.”, J. Acute Med. vol. 3, pp. 77-82, September 2012.
[11] D. Penduka, N. P. Gasa, M. S. Hlongwane, R. A. Mosa, F. O. Osunsanmi, and A. R. Opoku, “The antibacterial activities of some plant-derived triterpenes” afr j tradit complem. vol. 12, pp. 180-188, September 2015.
[12] R. A. Mosa, Nhleko, M.L., Dladla, T.V. and Opoku, A.R, “Antibacterial activity of two triterpenes from stem bark of Protorhus longifolia”, J. Med. Plant Res. vol. 8, pp. 686-702, May 2014.
[13] M. M. Suleiman, L. I. McGaw, V. Naidoo and J. Eloff, “Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species” AFR J TRADIT COMPLEM. vol. 7, pp. 64-78, October 2009.
[14] R. A. Mosa, G. G. Lazarus, P. E. Gwala, A. O. Oyedeji, and A. R. Opoku, “In vitro anti-platelet aggregation, antioxidant and cytotoxic activity of extracts of some Zulu medicinal plants”, J Nat Prod. vol. 4, pp. 136-146, June 2011.
[15] C. I. Sajeeth, “Quantitative estimation of gallic acid, rutin and quercetin in certain herbal plants by HPTLC method”, Der Chemica Sinica. vol. 1, pp. 80-85, 2010.
[16] J. Bora, D. Syiem and S. Bhan, “Quantitative analysis of total phenolic, flavonoid”, J. Pharmacognosy Phytother. vol. 8, pp. 906-911, April 2019.
[17] H. Imtara, et al, “Chemical analysis and cytotoxic and cytostatic Effects of twelve honey samples collected from different regions in morocco and Palestine”, Evid Based Complement Alternat Med. vol. 2019, pp. 1-11, May 2019.
[1] R. A. Street and G. Prinsloo, “Commercially important medicinal plants of South Africa”, J. Chem. vol. 2013, pp. 1-16, November 2012.
[2] M. Saxena, J. Saxena, R Nema, D. Singh and A. Gupta “Phytochemistry of medicinal plants”, J Pharmacogn Phytochem. vol. 1, pp. 168-182, March 2013.
[3] Y. A. Barku, Wound Healing-Current Perspectives. IntechOpen, 2019, ch.5.
[4] M. F. Mahomoodally, “Traditional medicines in Africa: An appraisal of ten potent African medicinal plants”, Evid Based Complement Alternat Med. vol. 2013, pp. 1-14, October 2013.
[5] S. Van Vuuren and D. Holl, "Antimicrobial natural product research: a review from a South African perspective for the years 2009–2016”, J. Ethnopharmacol. vol. 208, pp. 236-252, August 2017.
[6] L. V. Buwa-Komoren, B. Mayekiso, Z. Mhinana, and A. L. Adeniran, “An ethnobotanical and ethnomedicinal survey of traditionally used medicinal plants in Seymour, South Africa: An attempt toward digitization and preservation of ethnic knowledge”, Pharmacogn. Mag. vol. 15, pp. 115-123, January 2019.
[7] S. H. Nile and Park, S. W, “HPTLC analysis, antioxidant and antigout activity of Indian plants. Iran J Pharm Res. vol. 13, pp. 531-539, September 2014.
[8] L. Seasotiya, P. Siwach, A. Malik, S. Bai, P. Bharti and S. Dalal, “ Phytochemical evaluation and HPTLC fingerprint profile of Cassia fistula”, Int. J. Adv. Pharm., Biol. Chem. vol. 3, pp. 604-611, September 2014.
[9] A. K. Chandrakar, “Phytochemical fingerprinting through computerized HPTLC System for quality control of herbal drugs”, nt. j. res. appl. sci. eng. technol. vol. 6, pp. 733-738, January 2019.
[10] D. Gomathi, G. Ravikumar, M. Kalaiselvi, B. Vidya and C. Uma, “HPTLC fingerprinting analysis of Evolvulus alsinoides (L.) L.”, J. Acute Med. vol. 3, pp. 77-82, September 2012.
[11] D. Penduka, N. P. Gasa, M. S. Hlongwane, R. A. Mosa, F. O. Osunsanmi, and A. R. Opoku, “The antibacterial activities of some plant-derived triterpenes” afr j tradit complem. vol. 12, pp. 180-188, September 2015.
[12] R. A. Mosa, Nhleko, M.L., Dladla, T.V. and Opoku, A.R, “Antibacterial activity of two triterpenes from stem bark of Protorhus longifolia”, J. Med. Plant Res. vol. 8, pp. 686-702, May 2014.
[13] M. M. Suleiman, L. I. McGaw, V. Naidoo and J. Eloff, “Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species” AFR J TRADIT COMPLEM. vol. 7, pp. 64-78, October 2009.
[14] R. A. Mosa, G. G. Lazarus, P. E. Gwala, A. O. Oyedeji, and A. R. Opoku, “In vitro anti-platelet aggregation, antioxidant and cytotoxic activity of extracts of some Zulu medicinal plants”, J Nat Prod. vol. 4, pp. 136-146, June 2011.
[15] C. I. Sajeeth, “Quantitative estimation of gallic acid, rutin and quercetin in certain herbal plants by HPTLC method”, Der Chemica Sinica. vol. 1, pp. 80-85, 2010.
[16] J. Bora, D. Syiem and S. Bhan, “Quantitative analysis of total phenolic, flavonoid”, J. Pharmacognosy Phytother. vol. 8, pp. 906-911, April 2019.
[17] H. Imtara, et al, “Chemical analysis and cytotoxic and cytostatic Effects of twelve honey samples collected from different regions in morocco and Palestine”, Evid Based Complement Alternat Med. vol. 2019, pp. 1-11, May 2019.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:79345", author = "P. S. Seboletswe and Z. Mkhize and L. M. Katata-Seru", title = "HPTLC Fingerprint Profiling of Protorhus longifolia Methanolic Leaf Extract and Qualitative Analysis of Common Biomarkers", abstract = "Protorhus longifolia is known as a medicinal plant that has been used traditionally to treat various ailments such as hemiplegic paralysis, blood clotting related diseases, diarrhoea, heartburn, etc. The study reports a High-Performance Thin Layer Chromatography (HPTLC) fingerprint profile of Protorhus longifolia methanolic extract and its qualitative analysis of gallic acid, rutin, and quercetin. HPTLC analysis was achieved using CAMAG HPTLC system equipped with CAMAG automatic TLC sampler 4, CAMAG Automatic Developing Chamber 2 (ADC2), CAMAG visualizer 2, CAMAG Thin Layer Chromatography (TLC) scanner and visionCATS CAMAG HPTLC software. Mobile phase comprising toluene, ethyl acetate, formic acid (21:15:3) was used for qualitative analysis of gallic acid and revealed eight peaks while the mobile phase containing ethyl acetate, water, glacial acetic acid, formic acid (100:26:11:11) for qualitative analysis of rutin and quercetin revealed six peaks. HPTLC sillica gel 60 F254 glass plates (10 × 10) were used as the stationary phase. Gallic acid was detected at the Rf = 0.35; while rutin and quercetin were not evident in the extract. Further studies will be performed to quantify gallic acid in Protorhus longifolia leaves and also identify other biomarkers.
", keywords = "Biomarkers, fingerprint profiling, gallic acid, HPTLC, Protorhus longifolia.", volume = "13", number = "12", pages = "553-5", }
