Genetic Polymorphism of Main Lactoproteins of Romanian Grey Steppe Breed in Preservation
The paper presents a part of the results obtained in a
complex research project on Romanian Grey Steppe breed, owner of
some remarkable qualities such as hardiness, longevity, adaptability,
special resistance to ban weather and diseases and included in the
genetic fund (G.D. no. 822/2008.) from Romania.
Following the researches effectuated, we identified alleles of six
loci, codifying the six types of major milk proteins: alpha-casein S1
(α S1-cz); beta-casein (β-cz); kappa-casein (K-cz); beta-lactoglobulin
(β-lg); alpha-lactalbumin (α-la) and alpha-casein S2 (α S2-cz). In
system αS1-cz allele αs1-Cn B has the highest frequency (0.700), in
system β-cz allele β-Cn A2 ( 0.550 ), in system K-cz allele k-CnA2 (
0.583 ) and heterozygote genotype AB ( 0.416 ) and BB (0.375), in
system β-lg allele β-lgA1 has the highest frequency (0.542 ) and
heterozygote genotype AB ( 0.500 ), in system α-la there is
monomorphism for allele α-la B and similarly in system αS2-cz for
allele αs2-Cn A.
The milk analysis by the isoelectric focalization technique (I.E.F.)
allowed the identification of a new allele for locus αS1-casein, for two
of the individuals under analysis, namely allele called αS1-casein
IRV. When experiments were repeated, we noticed that this is not a
proteolysis band and it really was a new allele that has not been
registered in the specialized literature so far. We identified two
heterozygote individuals, carriers of this allele, namely: BIRV and
CIRV. This discovery is extremely important if focus is laid on the
national genetic patrimony.
[1] ┼×t. Creangâ., V. Maciuc, M. P├«ntea : Polimorfismul genetic ├«n ameliorarea
bovinelor din zona Moldovei. Editura ÔÇ×Ion Ionescu de la Brad", Ia┼ƒi, 2008,
pp. 23 - 65.
[2] V. Maciuc, ┼×t. Creangâ, M. P├«ntea : Ameliorarea geneticâ a bovinelor ├«n
regiunrea transfrontalierâ. Editura ÔÇ×Ion Ionescu de la Brad", Ia┼ƒi, 2008, pp.
166 - 175.
[3] I. Hekken, s. a. : Functional properties of dephosphorylated bovine whole
casein., J. Dairy Science, nr. 11, 1993.
[4] C., J. Mercier, s. a. : Génétique moléculaire des protéines du lait et de
leurs gènes. Biologie de la lactation. ├ëditions INRA., 1993.
[5] B. Ribadeau-Dumas : Protèines du lait i structure et fonctions. Biologie de
la lactation. Édition INRA, 1993.
[6] ┼×t. Creangâ, V. Maciuc, M. P├«ntea, I. G├«lcâ, R. Danaila : Romanian
Grey Steppe cattle breed in the context of genetic resources
preservation in Romania. 3-rd International Symposium, 18-20
September, University of Technology and Life Sciences in Bydgoszcz,
Poland. Book of abstract. Journal Central European of Agriculture,
2008, pp. 34 - 35.
[7] V. Râileanu : Sisteme polimorfe lactoproteice ┼ƒi influenţa lor asupra
proprietâţilor tehnologice ale laptelui. Tezâ de doctorat, U.A.S.M. Chi┼ƒinâu,
Republica Moldova, 2002.
[8] A., V. Balteanu, A. Vlaic, F., D. Pop, A., R. Rusu s.a. :
Characteriyation at protein level of the new ╬▒S1-cazein allele IRV
discovered in Romanian Grey Steppe cattle breed Moldavian variety.
Bulletin of USAMV - BT Anim. Sci. 4, 2008, pp. 1-10.
[9] A., V. Balteanu, A. Vlaic, A., R. Rusu, ┼×t. Creangâ, s.a. : Milk proteins
polymorphism in Romanian cattle breeds, identified by isoelectric
focusing technique (IEF). Bulletin of USAMV Iasi, Anim. Sci. 50, 2007,
pp.173-181.
[10] F. Grosclaude şi colab. : A mendelian polymorphism underlyng quantitative
variations of goat ╬▒S1-casein. Génét. Sél. Evol., 4, 1987, pp. 399 - 412.
[11] F. Grosclaude : Le polymorphisme génétique des principales lactoprotéines
bovines. INRA, Prod. Anim., 1, 1988, pp. 5 - 17.
[12] Ng-Kwai-Hang s. a. : Relationshisps between milk protein polymorphisms
and major milk constituants in Holstein-Friesian cows. J. Dairy Sci., 69,
1986, pp. 22-26.
[13] F., M. Mahé, G. Miranda, R., Queval, A. Bado, P.S. Zafindrajaona, and
F. Grosclaude : Genetic polymorphism of milk proteins in African Bos
taurus and Bos indicus populations. Characterization of variants aS1-
Cn H and k-Cn J. Genet. Sel. Evol. 31: 239 - 253, 1999.
[14] Y., Kawamoto, T.A. Namikawa, T. Adachi, T. Amano, T. Shotake, T.
Nishida, Y. Hayashi, B. Kattel, and H.B. Rajubhandary : A population
genetic study on yaks, cattle and their hybrids in Nepal using milk
protein variations. Anim. Sci. Tech. 63: 563 - 575, 1992.
[1] ┼×t. Creangâ., V. Maciuc, M. P├«ntea : Polimorfismul genetic ├«n ameliorarea
bovinelor din zona Moldovei. Editura ÔÇ×Ion Ionescu de la Brad", Ia┼ƒi, 2008,
pp. 23 - 65.
[2] V. Maciuc, ┼×t. Creangâ, M. P├«ntea : Ameliorarea geneticâ a bovinelor ├«n
regiunrea transfrontalierâ. Editura ÔÇ×Ion Ionescu de la Brad", Ia┼ƒi, 2008, pp.
166 - 175.
[3] I. Hekken, s. a. : Functional properties of dephosphorylated bovine whole
casein., J. Dairy Science, nr. 11, 1993.
[4] C., J. Mercier, s. a. : Génétique moléculaire des protéines du lait et de
leurs gènes. Biologie de la lactation. ├ëditions INRA., 1993.
[5] B. Ribadeau-Dumas : Protèines du lait i structure et fonctions. Biologie de
la lactation. Édition INRA, 1993.
[6] ┼×t. Creangâ, V. Maciuc, M. P├«ntea, I. G├«lcâ, R. Danaila : Romanian
Grey Steppe cattle breed in the context of genetic resources
preservation in Romania. 3-rd International Symposium, 18-20
September, University of Technology and Life Sciences in Bydgoszcz,
Poland. Book of abstract. Journal Central European of Agriculture,
2008, pp. 34 - 35.
[7] V. Râileanu : Sisteme polimorfe lactoproteice ┼ƒi influenţa lor asupra
proprietâţilor tehnologice ale laptelui. Tezâ de doctorat, U.A.S.M. Chi┼ƒinâu,
Republica Moldova, 2002.
[8] A., V. Balteanu, A. Vlaic, F., D. Pop, A., R. Rusu s.a. :
Characteriyation at protein level of the new ╬▒S1-cazein allele IRV
discovered in Romanian Grey Steppe cattle breed Moldavian variety.
Bulletin of USAMV - BT Anim. Sci. 4, 2008, pp. 1-10.
[9] A., V. Balteanu, A. Vlaic, A., R. Rusu, ┼×t. Creangâ, s.a. : Milk proteins
polymorphism in Romanian cattle breeds, identified by isoelectric
focusing technique (IEF). Bulletin of USAMV Iasi, Anim. Sci. 50, 2007,
pp.173-181.
[10] F. Grosclaude şi colab. : A mendelian polymorphism underlyng quantitative
variations of goat ╬▒S1-casein. Génét. Sél. Evol., 4, 1987, pp. 399 - 412.
[11] F. Grosclaude : Le polymorphisme génétique des principales lactoprotéines
bovines. INRA, Prod. Anim., 1, 1988, pp. 5 - 17.
[12] Ng-Kwai-Hang s. a. : Relationshisps between milk protein polymorphisms
and major milk constituants in Holstein-Friesian cows. J. Dairy Sci., 69,
1986, pp. 22-26.
[13] F., M. Mahé, G. Miranda, R., Queval, A. Bado, P.S. Zafindrajaona, and
F. Grosclaude : Genetic polymorphism of milk proteins in African Bos
taurus and Bos indicus populations. Characterization of variants aS1-
Cn H and k-Cn J. Genet. Sel. Evol. 31: 239 - 253, 1999.
[14] Y., Kawamoto, T.A. Namikawa, T. Adachi, T. Amano, T. Shotake, T.
Nishida, Y. Hayashi, B. Kattel, and H.B. Rajubhandary : A population
genetic study on yaks, cattle and their hybrids in Nepal using milk
protein variations. Anim. Sci. Tech. 63: 563 - 575, 1992.
@article{"International Journal of Biological, Life and Agricultural Sciences:53747", author = "Şt. Creangâ and V. Maciuc and A.V. Bâlteanu and S.S. Chelmu", title = "Genetic Polymorphism of Main Lactoproteins of Romanian Grey Steppe Breed in Preservation", abstract = "The paper presents a part of the results obtained in a
complex research project on Romanian Grey Steppe breed, owner of
some remarkable qualities such as hardiness, longevity, adaptability,
special resistance to ban weather and diseases and included in the
genetic fund (G.D. no. 822/2008.) from Romania.
Following the researches effectuated, we identified alleles of six
loci, codifying the six types of major milk proteins: alpha-casein S1
(α S1-cz); beta-casein (β-cz); kappa-casein (K-cz); beta-lactoglobulin
(β-lg); alpha-lactalbumin (α-la) and alpha-casein S2 (α S2-cz). In
system αS1-cz allele αs1-Cn B has the highest frequency (0.700), in
system β-cz allele β-Cn A2 ( 0.550 ), in system K-cz allele k-CnA2 (
0.583 ) and heterozygote genotype AB ( 0.416 ) and BB (0.375), in
system β-lg allele β-lgA1 has the highest frequency (0.542 ) and
heterozygote genotype AB ( 0.500 ), in system α-la there is
monomorphism for allele α-la B and similarly in system αS2-cz for
allele αs2-Cn A.
The milk analysis by the isoelectric focalization technique (I.E.F.)
allowed the identification of a new allele for locus αS1-casein, for two
of the individuals under analysis, namely allele called αS1-casein
IRV. When experiments were repeated, we noticed that this is not a
proteolysis band and it really was a new allele that has not been
registered in the specialized literature so far. We identified two
heterozygote individuals, carriers of this allele, namely: BIRV and
CIRV. This discovery is extremely important if focus is laid on the
national genetic patrimony.", keywords = "allele, breed, genetic preservation, lactoproteins,Romanian Grey Steppe", volume = "4", number = "5", pages = "264-5", }