Evaluation of the Antifungal and Antioxidant Activities of the Leaf Extract of Aloe vera(Aloe barbadensis Miller)
Aloe vera has been used worldwide both for
pharmaceutical, food, and cosmetic industries due to the plethora of
biological activities of some of its metabolites. The aim of this study
was to evaluate the antifungal and antioxidant activities of the leaf
extract. The antifungal activity was determined by the agar-well
diffusion method against plant and human fungal pathogens. The
methanol and ethanol portions of the extracts studied were more
bioactive than ethyl acetate portion. It was also observed that the
activity was more pronounced on plant pathogen than human
pathogen except Candida albicans. This is an indication that the
extract has the potential to treat plant fungal infections. The Aloe
extract showed the significant antioxidant activity by the DPPH
radical scavenging method. Therefore, the Aloe extract provided as
natural antioxidant has been used in health foods for medical and
preservative purposes.
[1] B. Mahesh and S. Satish, "Antimicrobial activity of some important
medicinal plant against plant and human pathogens," World J. Agri.Sci.,
vol. 4, no. 5, pp. 839-843, 2008.
[2] B. Halliwell, R. Aeschbach, J. Lo Liger and O.I. Aruoma, "The
characterization of antioxidant," Food Chem. Toxicol., vol. 33, pp. 601-
617, 1995.
[3] A. Mann, A. Banso , L.C. Clifford, "An Antifungal property of crude
plant extracts from Anogeissus leiocarpus and Terminalia
avicennioides," Tanzania J. Health Res., vol.10, no. 1, pp. 34-38, 2008.
[4] M. B. Ibrahim, "Anti-microbial effects of extract leaf, stem and root bark
of Anogeissus leiocarpus on Staphylococcus aureaus, Streptococcus
pyogenes, Escherichia coli and Proteus vulgaris," J. Pharma. Devpt.,
vol. 2, pp. 20-30, 1997.
[5] O. Ogundipe, O. Akinbiyi, J. O. Moody, "Antibacterial activities of
essential ornamental plants," Nigeria J. Natural Products & Medicine.,
vol. 2, pp. 46-47, 1998.
[6] T. Reynolds, Bot. J. Linn. Soc., vol. 90, pp. 157, 1985.
[7] D. Grindly, T. Reynolds, J. Ethnopharmacology, vol. 16, pp. 117, 1986.
[8] Jr. J. S. Haller, Bulletin of the New York Academy of Medicine, vol. 66,
pp. 647, 1990.
[9] U. Eggli, Illustrated Handbook of Succulent Plants: Monocotyledons,
Springer, 2001.
[10] L.G. Plaskett, "Aloe vera against infections", Newsletter 9, Publ.
Biomedical Information Services Ltd., Launceston, Cornwall, UK, 1997.
[11] K. Fujita, R. Teradaira, T. Nagatsu, "Bradykinase activity in Aloe
extract", Biochemical Pharmacology, vol. 25, pp. 205, 1976.
[12] K. R. Anilakumar, K. R. Sudarshanakrishna, G. Chandramohan,
Ilaiyaraja, K. Farhath, A. S. Bawa, "Effect of Aloe vera gel extract on
antioxidant enzymes and azoxymethane-induced oxidative stress in
rats," Ind. J. Expt. Biol., vol. 48, pp. 837-842, 2010.
[13] B. Vazquez, G. Avila, D. Segura, B. Edcalante, "Antiinflammatory
activity of extracts from Aloe vera gel," J. Ethanopharmacol, vol . 55,
pp. 9-15, 1996.
[14] Yun, N. Nasi, Lee, C. H. Chan-Ho, S. M. Sun-Meec, "Aloe vera could
be a potential therapeutic agent for the clinical treatment of sepsis,"
Food Chem. Toxicol., vol. 47, pp. 1341-1350, 2009.
[15] A. B. K. Chandan, Z. A. K Saxena, S. Sukla, N. Sharma, D. K. Gupta,
K. A. Suri, J. Suri, M. Bahauria, B. Singh, " Hepatoprotective potential
of Aloe barbedensis Mill. Against carbon tetrachloride induced
heptatotoxicity," J. Ethanopharmacol., vol. 11, pp. 560-569, 2007.
[16] L. Zhang, I. R. Tizard, "Activation of a mouse microphage cell line by
acemannan: the major carbohydrate fraction from Aloe vera gel,"
Immuno. Phar., vol. 35, pp. 119-128, 1996.
[17] P. Amruta , G. K. Purushottam, " Alteration in the antioxidant Potential
of Aloe vera Due to Fungal Infection," Plant Pathology J., vol. 6, no. 2,
pp. 169-173, 2007.
[18] W. B. Williams, M. Cuvelier, C. Berset, "Use of a free radical method
to evaluate antioxidant activity," Lebensm-Wiss Technol, vol. 28, pp. 25-
30, 1995.
[19] A. B. Ribeiro, V. S. Bolzani, M. Yoshida, L. S. Santos, M. N. Eberlin,
D. H. S. Silva, "A new neolignan and antioxidant phenols from
Nectandra grandiflora," J. Braz. Chem. Soc., Vol. 16, pp. 526-530,
2005.
[20] D. Jasso de Rodriguez, D. Hernandez-Castillo, R. Rodriguez-Garcia, J.
L. Angulo-Sanchez, "Antifungal activity in vitro of Aloe vera pulp and
liquid fraction against plant pathogenic fungi," Ind. Crops Prod., vol.
21, no. 1, pp. 81-87, 2005.
[21] V. Saritha, K. R. Anilakumar, F. Khanum, "Antioxidant and
antibacterial activity of Aloe vera gel extracts," Int. J. Pharmaceutical
&Bio. Arch., vol. 1, no. 4, pp. 376-384, 2010.
[1] B. Mahesh and S. Satish, "Antimicrobial activity of some important
medicinal plant against plant and human pathogens," World J. Agri.Sci.,
vol. 4, no. 5, pp. 839-843, 2008.
[2] B. Halliwell, R. Aeschbach, J. Lo Liger and O.I. Aruoma, "The
characterization of antioxidant," Food Chem. Toxicol., vol. 33, pp. 601-
617, 1995.
[3] A. Mann, A. Banso , L.C. Clifford, "An Antifungal property of crude
plant extracts from Anogeissus leiocarpus and Terminalia
avicennioides," Tanzania J. Health Res., vol.10, no. 1, pp. 34-38, 2008.
[4] M. B. Ibrahim, "Anti-microbial effects of extract leaf, stem and root bark
of Anogeissus leiocarpus on Staphylococcus aureaus, Streptococcus
pyogenes, Escherichia coli and Proteus vulgaris," J. Pharma. Devpt.,
vol. 2, pp. 20-30, 1997.
[5] O. Ogundipe, O. Akinbiyi, J. O. Moody, "Antibacterial activities of
essential ornamental plants," Nigeria J. Natural Products & Medicine.,
vol. 2, pp. 46-47, 1998.
[6] T. Reynolds, Bot. J. Linn. Soc., vol. 90, pp. 157, 1985.
[7] D. Grindly, T. Reynolds, J. Ethnopharmacology, vol. 16, pp. 117, 1986.
[8] Jr. J. S. Haller, Bulletin of the New York Academy of Medicine, vol. 66,
pp. 647, 1990.
[9] U. Eggli, Illustrated Handbook of Succulent Plants: Monocotyledons,
Springer, 2001.
[10] L.G. Plaskett, "Aloe vera against infections", Newsletter 9, Publ.
Biomedical Information Services Ltd., Launceston, Cornwall, UK, 1997.
[11] K. Fujita, R. Teradaira, T. Nagatsu, "Bradykinase activity in Aloe
extract", Biochemical Pharmacology, vol. 25, pp. 205, 1976.
[12] K. R. Anilakumar, K. R. Sudarshanakrishna, G. Chandramohan,
Ilaiyaraja, K. Farhath, A. S. Bawa, "Effect of Aloe vera gel extract on
antioxidant enzymes and azoxymethane-induced oxidative stress in
rats," Ind. J. Expt. Biol., vol. 48, pp. 837-842, 2010.
[13] B. Vazquez, G. Avila, D. Segura, B. Edcalante, "Antiinflammatory
activity of extracts from Aloe vera gel," J. Ethanopharmacol, vol . 55,
pp. 9-15, 1996.
[14] Yun, N. Nasi, Lee, C. H. Chan-Ho, S. M. Sun-Meec, "Aloe vera could
be a potential therapeutic agent for the clinical treatment of sepsis,"
Food Chem. Toxicol., vol. 47, pp. 1341-1350, 2009.
[15] A. B. K. Chandan, Z. A. K Saxena, S. Sukla, N. Sharma, D. K. Gupta,
K. A. Suri, J. Suri, M. Bahauria, B. Singh, " Hepatoprotective potential
of Aloe barbedensis Mill. Against carbon tetrachloride induced
heptatotoxicity," J. Ethanopharmacol., vol. 11, pp. 560-569, 2007.
[16] L. Zhang, I. R. Tizard, "Activation of a mouse microphage cell line by
acemannan: the major carbohydrate fraction from Aloe vera gel,"
Immuno. Phar., vol. 35, pp. 119-128, 1996.
[17] P. Amruta , G. K. Purushottam, " Alteration in the antioxidant Potential
of Aloe vera Due to Fungal Infection," Plant Pathology J., vol. 6, no. 2,
pp. 169-173, 2007.
[18] W. B. Williams, M. Cuvelier, C. Berset, "Use of a free radical method
to evaluate antioxidant activity," Lebensm-Wiss Technol, vol. 28, pp. 25-
30, 1995.
[19] A. B. Ribeiro, V. S. Bolzani, M. Yoshida, L. S. Santos, M. N. Eberlin,
D. H. S. Silva, "A new neolignan and antioxidant phenols from
Nectandra grandiflora," J. Braz. Chem. Soc., Vol. 16, pp. 526-530,
2005.
[20] D. Jasso de Rodriguez, D. Hernandez-Castillo, R. Rodriguez-Garcia, J.
L. Angulo-Sanchez, "Antifungal activity in vitro of Aloe vera pulp and
liquid fraction against plant pathogenic fungi," Ind. Crops Prod., vol.
21, no. 1, pp. 81-87, 2005.
[21] V. Saritha, K. R. Anilakumar, F. Khanum, "Antioxidant and
antibacterial activity of Aloe vera gel extracts," Int. J. Pharmaceutical
&Bio. Arch., vol. 1, no. 4, pp. 376-384, 2010.
@article{"International Journal of Medical, Medicine and Health Sciences:51755", author = "Tin A. Khaing", title = "Evaluation of the Antifungal and Antioxidant Activities of the Leaf Extract of Aloe vera(Aloe barbadensis Miller)", abstract = "Aloe vera has been used worldwide both for
pharmaceutical, food, and cosmetic industries due to the plethora of
biological activities of some of its metabolites. The aim of this study
was to evaluate the antifungal and antioxidant activities of the leaf
extract. The antifungal activity was determined by the agar-well
diffusion method against plant and human fungal pathogens. The
methanol and ethanol portions of the extracts studied were more
bioactive than ethyl acetate portion. It was also observed that the
activity was more pronounced on plant pathogen than human
pathogen except Candida albicans. This is an indication that the
extract has the potential to treat plant fungal infections. The Aloe
extract showed the significant antioxidant activity by the DPPH
radical scavenging method. Therefore, the Aloe extract provided as
natural antioxidant has been used in health foods for medical and
preservative purposes.", keywords = "Aloe vera, antifungal, antioxidant, DPPH", volume = "5", number = "3", pages = "99-3", }
