Effects of Chitosan as the Growth Stimulator for Grammatophyllum speciosum in Vitro Culture
The effects of chitosan, a biodegradable polymer,
were studied in Grammatophyllum speciosum protocorm-like bodies
(PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15,
20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige
and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v)
sucrose. The results showed that liquid medium supplemented with
15 mg/l chitosan showed the highest relative growth rate (7-fold
increase) of PLBs. On 1/2 MS agar medium supplemented with 25
mg/l chitosan gave the highest relative growth rate (4-fold increase).
The relative growth rate of G. speciosum PLBs on agar medium was
significantly lower than that in liquid medium. Moreover, chitosan,
supplemented to agar medium promoted shoot formation but not
rooting. However, supplementation at too high a level, such as 100
mg/l can inhibit growth and kill PLBs.
[1] A. Pridgeon, The Illustrated Encyclopedia of Orchid. Kyodo Printing Pte
Ltd, Devon, Singapore, 2003.
[2] G.M. Morel, Producing virus free Cymbidium. Am Orchid SocBull , vol.
29, pp. 495-497, 1960.
[3] K.L. Nge, N. Nwe, S. Chandrkrachang and S. Stevens, Chitosan as a
growth stimulator in orchid tissue culture. PlantSci. vol. 170, pp. 1185-
1190, 2005.
[4] E.A. Barka, P. Eullaffroy, C. Clement and G. Vernet, Chitosan improves
development and protects Vitis vinifera L. against Borytis cinerea. Plant
Cell Rep, vol,. 22, pp. 608-614, 2004.
[5] K. Sopalun, K. Thammasiri and K. Ishikawa, Micropropagation of the
Thai orchid Grammatophyllum speciosum blume. Plant Cell Tiss Organ
Cult, vol. 101, pp. 143-150, 2010.
[6] A. Uthairatanakij, J.A. Teixeira da Silva and K. Obi Wan, Chitosan for
improving orchid production and quality. Orchid Sci Biotechnol, vol. 1,
pp. 1-5, 2007.
[1] A. Pridgeon, The Illustrated Encyclopedia of Orchid. Kyodo Printing Pte
Ltd, Devon, Singapore, 2003.
[2] G.M. Morel, Producing virus free Cymbidium. Am Orchid SocBull , vol.
29, pp. 495-497, 1960.
[3] K.L. Nge, N. Nwe, S. Chandrkrachang and S. Stevens, Chitosan as a
growth stimulator in orchid tissue culture. PlantSci. vol. 170, pp. 1185-
1190, 2005.
[4] E.A. Barka, P. Eullaffroy, C. Clement and G. Vernet, Chitosan improves
development and protects Vitis vinifera L. against Borytis cinerea. Plant
Cell Rep, vol,. 22, pp. 608-614, 2004.
[5] K. Sopalun, K. Thammasiri and K. Ishikawa, Micropropagation of the
Thai orchid Grammatophyllum speciosum blume. Plant Cell Tiss Organ
Cult, vol. 101, pp. 143-150, 2010.
[6] A. Uthairatanakij, J.A. Teixeira da Silva and K. Obi Wan, Chitosan for
improving orchid production and quality. Orchid Sci Biotechnol, vol. 1,
pp. 1-5, 2007.
@article{"International Journal of Biological, Life and Agricultural Sciences:54760", author = "Sopalun K. and Thammasiri K. and Ishikawa K.", title = "Effects of Chitosan as the Growth Stimulator for Grammatophyllum speciosum in Vitro Culture", abstract = "The effects of chitosan, a biodegradable polymer,
were studied in Grammatophyllum speciosum protocorm-like bodies
(PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15,
20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige
and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v)
sucrose. The results showed that liquid medium supplemented with
15 mg/l chitosan showed the highest relative growth rate (7-fold
increase) of PLBs. On 1/2 MS agar medium supplemented with 25
mg/l chitosan gave the highest relative growth rate (4-fold increase).
The relative growth rate of G. speciosum PLBs on agar medium was
significantly lower than that in liquid medium. Moreover, chitosan,
supplemented to agar medium promoted shoot formation but not
rooting. However, supplementation at too high a level, such as 100
mg/l can inhibit growth and kill PLBs.", keywords = "Chitosan, Grammatophyllum speciosum, Growth
stimulator", volume = "4", number = "11", pages = "815-3", }