Effect of Plasma Therapy on Epidermal Regeneration
The purpose of our study was to compare spontaneous
re-epithelisation characteristics versus assisted re-epithelisation. In
order to assess re-epithelisation of the injured skin, we have imagined
and designed a burn wound model on Wistar rat skin. Our aim was to
create standardised, easy reproducible and quantifiable skin lesions
involving entire epidermis and superficial dermis. We then have
applied the above mentioned therapeutic strategies to compare
regeneration of epidermis and dermis, local and systemic parameter
changes in different conditions. We have enhanced the reepithelisation
process under a moist atmosphere of a polyurethane
wound dress modified with helium non-thermal plasma, and with the
aid of direct cold-plasma treatment respectively. We have followed
systemic parameters change: hematologic and biochemical
parameters, and local features: oxidative stress markers and histology
of skin in the above mentioned conditions. Re-epithelisation is just a
part of the skin regeneration process, which recruits cellular
components, with the aid of epidermal and dermal interaction via
signal molecules.
[1] Alvarez, O.M,, Mertz PM, Eaglestein WH. The effect of occlusive
dressings on collagen synthesis and re-epithelialization in superficial
wounds. J Surg Res 35: 142-8; 1983.
[2] Arturson, G. Pathophysiology of the burn wound. Ann Chir Gyn; 69:
178; 1980.
[3] Bothwell, J.W., Rovee DT, Downes AM, Flanagan PA, Kurowsky CA.
The effects of the climate on the repair of cutaneous wounds in humans.
In: Maibach HI, Rovee DT, eds. Epidermal wound healing. Chicago:
Year Book Medical Publishers, Inc., 255-66; 1972.
[4] Cross, K.J., Mustoe TA - Growth Factors in Wound Healing, Surg Clin
N Am, 83 531-545; 2003.
[5] Eaglestein, W.H., Davis SC, Mchlc AL, Mertz PM. Optimal use of an
occlusive dressing to enhance healing. Arch Dermatol, 1988.
[6] Friedman, G., Peddinghaus M, Fridman A, Balasubramanian M, Gutsol
A, Friedman G. Use of Non-Thermal Atmospheric Pressure Plasma
Discharge for Coagulation and Steriliztion of Surface Wounds, Plasma
Chemistry and Plasma Processing, 2006.
[7] Friedman, G; Chirokov, et al., Non-Thermal Atmospheric Pressure
Discharges, Journal of Physics D-Applied Physics, 2005.
[8] Hermans, M.H.E., Hermans RP. Duoderm, an alternative dressing for
smaller burns. Burns 1986; 12: 214-9;
[9] Krawcyk, WS., A pattern of epidermal cell migration during wound
healing. J Cell Biol; 49: 247-63. 99; 1971.
[10] May, S.R., Physiology, immunology, and clinical efficacy of an
adherent polyurethane wound dressing: Opsitc, Wise DL, cd. Burn
wound coverings. Boca Raton: CRC Press, Inc., 53-78; 1984.
[11] May, S.R., Properties of an adherent polyurcthanc wound dressing, Care
of the burn wound. Basel: Kargcr, 204-17, 1985.
[12] Miller, T.A., The healing of partial-thickness skin injuries. In: Hunt
KH,ed. Wound healing and wound infection. New York: Appleton-
Century-Crofts, 81-96; 1980.
[13] Petrie, N.C., Feng Yao, Eriksson E - Gene Therapy in Wound Healing,
Surg Clin N Am, 83, 597-616; 2003.
[14] Schwentker, A., Timothy, R., - Billiar-Nitric Oxide and Wound Repair,
Surg Clin N Am 83, 521-530; 2003.
[15] Sosnin, Stoffels, et al., The Effects of Uv Irradiation and Gas Plasma
Treatment on Living Mammalian Cells and Bacteria: A comparatice
Approach, Ieee Transactions on Plasma Science, 2004.
[16] Winter, G.D., A note on wound healing under dressings with special
reference to perforated-film dressings. J Invest Dermatol; 45: 299-302;
1965.
[17] Winter, G.D., Formation of the scab and the rate of cpithclization of
superficial wounds in the skin of the young domestic pig. Nature
(Lond); 193: 293-4; 1962.
[18] Winter, G.D., Healing of skin wounds and the influence of dressings on
the repair process. In: Harkiss KJ, ed. Surgical dressings and wound
healing. Bradford: Bradford University Press, 46-60; 1971.
[19] Zawacki, B.E., Reversal of capillary statis and prevention of necrosis in
burns. Ann Surg; 180: 98-102; 1974.
[20] Ebell, B., The Papyrus Ebers. The greatest Egyptian medical document.
Coopenhagen: Levin and Munlsgaard, 1937.
[21] Stickel, D.L., Seigler HF. Transplantation. I. Historical aspects. In:
Sabiston DC, Textbook of Surgery, 11th ed. Philadelphia: WB Saunders
Company, 4:56-63, 1977.
[22] Vasilets, V.N., A. Gutsol, A.B. Shekhter, A. Fridman. Plasma Medicine.
[23] High Energy Chemistry, v.43 (N3) pp.229-233, 2009.
[24] Gregory Fridman, Victor N.Vasilets, Alexander Gutsol, Gary Friedman,
Anatoly B.Shekhter, Alexander Fridman. Applied Plasma Medicine.
Plasma Processes and Polymers, N5, pp.503-533, 2008.
[25] Yildirim Eda D., Halim Ayan, Victor N.Vasilets, Alexander Fridman,
Selcuk Guceri, Wei Sun. Effect of Dielectric Barrier Discharge Plasma
on the Attachment And Proliferation of Osteoblasts Cultured over Poly
(e-Caprolactone) Scaffolds.
[1] Alvarez, O.M,, Mertz PM, Eaglestein WH. The effect of occlusive
dressings on collagen synthesis and re-epithelialization in superficial
wounds. J Surg Res 35: 142-8; 1983.
[2] Arturson, G. Pathophysiology of the burn wound. Ann Chir Gyn; 69:
178; 1980.
[3] Bothwell, J.W., Rovee DT, Downes AM, Flanagan PA, Kurowsky CA.
The effects of the climate on the repair of cutaneous wounds in humans.
In: Maibach HI, Rovee DT, eds. Epidermal wound healing. Chicago:
Year Book Medical Publishers, Inc., 255-66; 1972.
[4] Cross, K.J., Mustoe TA - Growth Factors in Wound Healing, Surg Clin
N Am, 83 531-545; 2003.
[5] Eaglestein, W.H., Davis SC, Mchlc AL, Mertz PM. Optimal use of an
occlusive dressing to enhance healing. Arch Dermatol, 1988.
[6] Friedman, G., Peddinghaus M, Fridman A, Balasubramanian M, Gutsol
A, Friedman G. Use of Non-Thermal Atmospheric Pressure Plasma
Discharge for Coagulation and Steriliztion of Surface Wounds, Plasma
Chemistry and Plasma Processing, 2006.
[7] Friedman, G; Chirokov, et al., Non-Thermal Atmospheric Pressure
Discharges, Journal of Physics D-Applied Physics, 2005.
[8] Hermans, M.H.E., Hermans RP. Duoderm, an alternative dressing for
smaller burns. Burns 1986; 12: 214-9;
[9] Krawcyk, WS., A pattern of epidermal cell migration during wound
healing. J Cell Biol; 49: 247-63. 99; 1971.
[10] May, S.R., Physiology, immunology, and clinical efficacy of an
adherent polyurethane wound dressing: Opsitc, Wise DL, cd. Burn
wound coverings. Boca Raton: CRC Press, Inc., 53-78; 1984.
[11] May, S.R., Properties of an adherent polyurcthanc wound dressing, Care
of the burn wound. Basel: Kargcr, 204-17, 1985.
[12] Miller, T.A., The healing of partial-thickness skin injuries. In: Hunt
KH,ed. Wound healing and wound infection. New York: Appleton-
Century-Crofts, 81-96; 1980.
[13] Petrie, N.C., Feng Yao, Eriksson E - Gene Therapy in Wound Healing,
Surg Clin N Am, 83, 597-616; 2003.
[14] Schwentker, A., Timothy, R., - Billiar-Nitric Oxide and Wound Repair,
Surg Clin N Am 83, 521-530; 2003.
[15] Sosnin, Stoffels, et al., The Effects of Uv Irradiation and Gas Plasma
Treatment on Living Mammalian Cells and Bacteria: A comparatice
Approach, Ieee Transactions on Plasma Science, 2004.
[16] Winter, G.D., A note on wound healing under dressings with special
reference to perforated-film dressings. J Invest Dermatol; 45: 299-302;
1965.
[17] Winter, G.D., Formation of the scab and the rate of cpithclization of
superficial wounds in the skin of the young domestic pig. Nature
(Lond); 193: 293-4; 1962.
[18] Winter, G.D., Healing of skin wounds and the influence of dressings on
the repair process. In: Harkiss KJ, ed. Surgical dressings and wound
healing. Bradford: Bradford University Press, 46-60; 1971.
[19] Zawacki, B.E., Reversal of capillary statis and prevention of necrosis in
burns. Ann Surg; 180: 98-102; 1974.
[20] Ebell, B., The Papyrus Ebers. The greatest Egyptian medical document.
Coopenhagen: Levin and Munlsgaard, 1937.
[21] Stickel, D.L., Seigler HF. Transplantation. I. Historical aspects. In:
Sabiston DC, Textbook of Surgery, 11th ed. Philadelphia: WB Saunders
Company, 4:56-63, 1977.
[22] Vasilets, V.N., A. Gutsol, A.B. Shekhter, A. Fridman. Plasma Medicine.
[23] High Energy Chemistry, v.43 (N3) pp.229-233, 2009.
[24] Gregory Fridman, Victor N.Vasilets, Alexander Gutsol, Gary Friedman,
Anatoly B.Shekhter, Alexander Fridman. Applied Plasma Medicine.
Plasma Processes and Polymers, N5, pp.503-533, 2008.
[25] Yildirim Eda D., Halim Ayan, Victor N.Vasilets, Alexander Fridman,
Selcuk Guceri, Wei Sun. Effect of Dielectric Barrier Discharge Plasma
on the Attachment And Proliferation of Osteoblasts Cultured over Poly
(e-Caprolactone) Scaffolds.
@article{"International Journal of Medical, Medicine and Health Sciences:54423", author = "Magda Bădescu and Daniela Jitaru and C.Grigoraş and L.Bădescu and I. Topala and Manuela Ciocoiu", title = "Effect of Plasma Therapy on Epidermal Regeneration", abstract = "The purpose of our study was to compare spontaneous
re-epithelisation characteristics versus assisted re-epithelisation. In
order to assess re-epithelisation of the injured skin, we have imagined
and designed a burn wound model on Wistar rat skin. Our aim was to
create standardised, easy reproducible and quantifiable skin lesions
involving entire epidermis and superficial dermis. We then have
applied the above mentioned therapeutic strategies to compare
regeneration of epidermis and dermis, local and systemic parameter
changes in different conditions. We have enhanced the reepithelisation
process under a moist atmosphere of a polyurethane
wound dress modified with helium non-thermal plasma, and with the
aid of direct cold-plasma treatment respectively. We have followed
systemic parameters change: hematologic and biochemical
parameters, and local features: oxidative stress markers and histology
of skin in the above mentioned conditions. Re-epithelisation is just a
part of the skin regeneration process, which recruits cellular
components, with the aid of epidermal and dermal interaction via
signal molecules.", keywords = "Plasma medicine, re-epithelisation and tissue
regeneration", volume = "6", number = "4", pages = "81-6", }
