Effect of Different Methods to Control the Parasitic Weed Phelipanche ramosa (L.- Pomel) in Tomato Crop

Phelipanche ramosa is the most damaging obligate
flowering parasitic weed on wide species of cultivated plants. The
semi-arid regions of the world are considered the main centers of this
parasitic plant that causes heavy infestation. This is due to its
production of high numbers of seeds (up to 200,000) that remain
viable for extended periods (up to 20 years). In this study, 13
treatments for the control of Phelipanche were carried out, which
included agronomic, chemical, and biological treatments and the use
of resistant plant methods. In 2014, a trial was performed at the
Department of Agriculture, Food and Environment, University of
Foggia (southern Italy), on processing tomato (cv ‘Docet’) grown in
pots filled with soil taken from a field that was heavily infested by P.
ramosa). The tomato seedlings were transplanted on May 8, 2014,
into a sandy-clay soil (USDA). A randomized block design with 3
replicates (pots) was adopted. During the growing cycle of the
tomato, at 70, 75, 81 and 88 days after transplantation, the number of
P. ramosa shoots emerged in each pot was determined. The tomato
fruit were harvested on August 8, 2014, and the quantitative and
qualitative parameters were determined. All of the data were
subjected to analysis of variance (ANOVA) using the JMP software
(SAS Institute Inc. Cary, NC, USA), and for comparisons of means
(Tukey's tests). The data show that each treatment studied did not
provide complete control against P. ramosa. However, the virulence
of the attacks was mitigated by some of the treatments tried: radicon
biostimulant, compost activated with Fusarium, mineral fertilizer
nitrogen, sulfur, enzone, and the resistant tomato genotype. It is
assumed that these effects can be improved by combining some of
these treatments with each other, especially for a gradual and
continuing reduction of the “seed bank” of the parasite in the soil.

• G. Disciglio
• F. Lops
• A. Carlucci
• G. Gatta
• A. Tarantino
• E. Tarantino

• Control methods
• Phelipanche ramosa
• tomato crop.

[1] R. Zindahl, 1993. Fundamentals of weed science. Academic Press Inc.,
New York, pp. 499.
[2] K.H. Linke, J. Sauerborn, M.C. Saxena, 1989. Study on viability and
longevity of Orobanche seed under laboratory conditions. In: Progress in
Orobanche research (Eds. Wegmann K., Musselman L.J.), Eberhard-
Karls_Universitat, Tubingen, FRG, 110-114.
[3] Habimana S., Murthy K.N.K., Hatti V., Nduwumuremyi A., 2013.
Management of Orobanche in field crops. A review. Scientific Journal
of Crop Science, no 2(11), 144-158.
[4] D.M Joel, 2000. The long-term approach to parasitic weeds control,
manipulation of specific developmental mechanisms of the parasite.
Crop Prot., 19, 753-758.
[5] Y. Goldwasser, Y. Kleifeld, 2004. Recent approaches to Orobanche
management, A Review. Weed Biol. Manag., 439-466.
[6] Z. Alejandro, S. Barghouthi, B. Cohen, I. Goldwasser, J .Gressel, L.
Hornok, Z. Kerenyi, I. Kleifeld, O. Klein, J. Kroschel, J. Sauerborn, D.
Muller-Stover, H. Thomas, M. Vurro, M.C.H. Zonno, 2001. Recent
advances in the biocontrol of Orobanche (Broomrape) species. Bio-
Control, 46, 211-228.
[7] D. Rubiales, M. Fernandenz-Aparicio, 2012. Innovations in parasitic
weeds management in legume crops. Agron. Sustain. Dev., no.32, 433-
449.
[8] F.F. Bebawi, 1981. Response of Sorghum bicolor cultivars and
Strigahermonthica population to nitrogen fertilization. Plant Soil, 59,
261-268.
[9] M. Jamil, T. Charnikhova, C. Cardoso, T. Jamil, K. Ueno, F.
Verstappen, T. Asami, H.J. Bouwmeester, 2011. Quantification of the
relationship between strigolactones and Strigahermonthica infection in
rice under varying levels of nitrogen and phosphorus. Weed Res., 51,
373-385.
[10] B. E. Abu-Irmaileh, 1981. Response of hemp broomrape
(Orobancheramosa) infestation to some nitrogenous compounds. Weed
Sci., 29, 8-10.
[11] M.A. Haidar, M.M. Sidahmed, 2006. Elemental sulphur and chicken
manure for the control of branched broomrape (Orobancheramosa).
Crop Protection, 25, 47-51.
[12] M.A. Haidar, M.M. Sidahmed, 2000. Soil solarization and chicken
manure for the control of Orobanchecrenataand other weeds in
Lebanon. Crop Prot., 19, 169-173.
[13] A.M. Litterick, L.A. Harrier, P. Wallace, C.A.Watson, M. Wood, 2004.
The role of uncomposted materials, composts, manures and compost
extracts in reducing pest and disease incidence and severity in
sustainable temperate agricultural and horticultural crop production. A
review. Crit Rev Plant Sci 23, 453-479.
[14] G. Alfano, G. Lustrato, G. Lima, D. Vuitullo, G. Ranalli, 2011.
Characterization of composted olive-mill wastes to predict potential
plant disease suppressiveness. Biol. Control, 58, 199-207.
[15] S. Tardioli, E.T.G. Bannè, F. Santoi, 1997. Species-specific selection on
soil fungal population after olive miller wastewater treatment.
Chemosphere 34, 2329-2336.
[16] H. Saad, Y. Laor, M. Raviv, S. Medina, 2007. Land spreading of oliver
mill wastewater. Effects on soil microbial activity and potential
phytotoxicity. Chemosphere, 66, 75-83.
[17] G. Disciglio, G. Gatta, A. Libutti, A. Gagliardi, A., Carlucci, F. Lops, F.
Cibelli, A. Tarantino, 2015. Effects of irrigation with treated agroindustrial
wastewater on soil chemical characteristics and fungal
populations during processing tomato crop cycle (In press).
[18] Z.Y. Amsellem, S. Barghouthi, B. Cohen, Y. Goldwasser, J. Gressel, L.,
Hornok, Z. Kerenyi, Y. Kleifeld, O. Klein, J. Kroschel, J. Sauerborn, D.
Müller-Stöver, H. Thomas, M. Vurro, M.C. Zonno, 2001. Recent
advances in the biocontrol of Orobanche (broomrape) species,
Biocontrol, 46, 211-228.
[19] E. Dor, A. Evidente, C. Amalfitano, D. Agrelli, J. Hershenhorn, 2007.
The influence of growth conditions on biomass, toxins and pathogenicity
of Fusariumoxysporum f. sp. Orthoceras, a potential agent for broomrape
control. Weed Research, 47, 345-352.
[20] J. Sauerborn, D. Muller-Stover, J. Hershenhorn, 2007. The role of
biological control in managing parasitic weeds. Crop Prot. 26, 246-254.
[21] M.C. Zonno, M. Vurro, 2002. Inhibition of germination of
Orobancheramosa seeds by Fusariumtoxins Phytoparasitica, 30, 519-
524.
[22] I.E. Azam, M.A. Abouzeid, A. Boari, M. Vurro, A. Evidente, 2003.
Identification of phytotoxic metabolites of a new Fusarium species
inhibiting germination of Strigahermonthica seeds.
PhytopathologicMediterranea, 42, 65-70.
[23] A. Boari, M. Vurro, 2004. Evaluation of Fusarium spp. and other fungi
as biological control agents of Broomrape (Orobancheramosa). Biol.
Control, 30, 212-219.
[24] V.W. Lendzemo, T.W. Kuyper, R. Matusova, H.J. Bouwmeester, A.
Van Ast, 2007. Colonization by arbuscularmycorrhizal fungi of
sorghum leads to reduced germination and subsequent attachment and
emergence of Strigahermonthica. Plant Signalling and Behavior, 2, 1-5.
[25] M.A. Abouzeid, K.A., El-Tarabily, 2010. Fusarium spp. Suppress
germination and parasitic establishment of bean and hemp broomrapes.
Phytopathol. Mediterr., 49, 51-64.
[26] E. Dor, B. Alperin, S. Wininger, B.,Ben-Dor, V.S. Somvanshi, H.
Koltai, Y. Kapulnik, J. Hershenhorn, 2010. Characterization of a novel
tomato mutant resistant to the weedy parasites Orobanche and
Phelipanche spp.Euphytica, 171, 371-380.
[27] J. Gressel, 2013. Biotechonologs for directly generating crop resistant to
parasite. In Joel D.M., Gressel J., Lytton J., Parasitic Orobanchaceae.
Parasitism mechanisms on control strategies. Musselman Editors. Cap.
24, 433-458.
[28] A. Caliandro, N. Lamaddalena, M. Stelluti, P. Steduto, 2005.
Caratterizzazione agro-ecologica della Regione Puglia in funzione della
potenzialità produttiva. Progetto ACLA. Ideaprint, BARI, Italy, ISBN:
2-85352-339-X.
[29] M. Jiménez-Cuesta, J. Cuquarella, J.M. Martinez-Javaga, 1981.
Determination of color index for Citrus fruits degrening. Proc. Int.
Citriculture, 2, 750-753.
[30] F.J. Francis F.M. Clydesdale, 1975. Food Colorimetry: Theory and
applications. AVI Publ. Co., Westport, CT. pp. 477.Fisher P.J., O.
Petrini, L.E., Petrini and E. Descals, 1992. A preliminary study of fungi
inhabiting xylem and whole stems of Oleaeuropaea. Sydowia 44, 117–
121.
[31] F. Favati, S. Lovelli, F. Galgano, V. Miccolis, T. Di Tommaso, V.
Candido, 2009. Processing tomato quality as affected by irrigation
scheduling. ScientiaHorticulturae, Vol. 122, No. 4, (November 2009),
562–571.
[32] M. Vurro, A. Boari, A.L. Pilgeram, D.C. Sands, 2005. Exogenous
amino acids inhibit seed germination and tubercle formation by
Orobancheramosa (Broomrape): potential application for management
of parasitic weed. Biological Control, 36, 258-265.
[33] M.A. Abouzeid, M.C. Boari, M. Zonno, M. Vurro, A. Evidente, 2004.
Toxicity profiles of potential biocontrol agents of Orobancheramosa.
Weed Science, 52, 326-332.
[34] J. Sauerborn, D. Rubiales, 2007. Biology and management of weedy
root parasites. In: Janick J. (Ed.) Horticultural Reviews. JohnWiley&
Sons Inc. USA, 267-349.
[35] K. Joneyama, X. Xie, K Yoneyama, Y. Takfuchi, 2009. Stigolactones:
structures and biological activities. Pest. Management science, 65, 467-
470.
[36] M.Fernandez-Aporicio, F. Flores, D. Rubiales, 2009. Recognition of
root exudates by seeds of broompare (Orobancheand Phelipanche)
species. Annals of Botany, 103, 423-431.
[37] Y. El-Halmouch, P. Thalouam, 2006. Effect of root exudates from
different tomato genotypes on broomrape (o. aegyptiaca) seed
germination and tubercle development. Crop Prot., 25, 501-507.[38] J.A Lopez-Ráez, R. Matusova, C. Cardoso, M. Jamil, T. Charnikhova,
W. Kohlen, C. Ruyter-Spira, F. Verstappen, H. Bouwmeester, 2008.
Stigolactones: ecological significance and use as a target for parasitic
plant control. Pest. Manag. Sci, 65, 471-477.
[39] X. Xie, D. Kusumoto, Y. Takeuchi, K. Yoneyama, Y. Yamada, K.
Yoneyama, 2007. 2’-Epi-orobanchol and solanacol, two unique
strigolactones, germination stimulants for root parasitic weeds, produced
by tobacco. J. Agric. Food Chem., 55-8067-8072.