Effect of Boric Acid on a-Hydroxy Acids Compounds in Thin Layer Chromatography
In this investigation Salicylic acid, Sulfosalicylic acid
and Acetyl salicylic acid were chosen as a sample for thin layer
chromatography (TLC) on silica gel plates. Bicarbonate buffer at
different pH containing different amounts of boric acid was applied
as mobile phase. Specific interaction of these substances with boric
acid has effect on Rf in thin layer chromatography. Regular and
similar trend was observed in variations of Rf for mentioned
compounds in TLC by altering of percentages of boric acid in mobile
phase in pH range of 8-10. Also effect of organic solvent, mixture of
water/ organic solvent and organic solvent containing boric acid as
mobile phase was studied.
[1] The Salt Collaborative Group. Lancet., vol. 388, pp. 1345-1349, 1991.
[2] M. Lecomte, O.J.C. Laneuville, D.L. Dewitt, W.L. Smith, J. Biochem.,
vol. 269, pp. 13207-13215, 1994.
[3] J.P. Metraux, H. Signer, J. Ryals, E. Ward, M. Wyss-Benz, G. Gaudin,
K. Raschdorf, E. Schmid, W. Blum, and B. Inverardi, Science, vol. 250,
pp. 1004-1006, 1990.
[4] J. Malamy, J.P. Carr, D.F. Klessig, and I. Raskin, Science, vol. 250, pp.
1002-1004, 1990.
[5] G.A. Higgs, J.A. Salmon, B. Henderson, and J.R. Vane, Proc. Natl.
Acad. Sci.USA., vol. 84, pp. 1417-1420, 1987.
[6] M.J .Thun, M.M. Namboodiri, E.E. Calle, W.D. Flanders, and C.W.
Heath, Cancer Res., vol. 53, pp. 122-1327, 1993.
[7] X.M. Xu, G, Sansores, A. Chenxm, B. Matijevik, N. Aleksic, P. Dum,
and A.Wukk, Proc. Nati. Acad. Sci. USA, vol. 96, pp. 5292-5297, 1999.
[8] S. Dongz, B. Huangc, R.E. Brown, and Z. Mawy, J. Biol. Chem. vol.
271, pp. G 722 -G 727, 1996.
[9] N.H. Pillinger, A. Capodicic, P. Rosenthal, N. Kheterpal, B. Hanfts,
M.R. Philips and G. Welssmann. Pro. Natl. Acad. Sci. USA., vol. 95,
pp. 14540-14545, 1998.
[10] P. Ricchi, S. Pignata, A. Dipoplo, A.M. Memoli, A. Apicellu, R. Zarrilli,
and A.M. Acquaviva, Int. J. cancer, vol. 73, pp. 880-884, 1997.
[11] P. Ricchi, T. Dimatola, A. Ruggieog , D. Zanzi, A. Apicellu, A.
Dipalma, M. Pensabene, S. Pignata, R. Zarrilli, and A.M. Acquaviva,
Brj. Cancer, vol. 86, pp. 1501-1509, 2002.
[12] B. K. Low, M. E. Waltner-Low, A. J. Entingh, A. Chytil, M.E. Akre, P.
Norgaard, and H.L. Moses, J. Biol. Chem., vol. 275, pp. 38261-38267,
2000.
[13] P. Ricchi, A.D. Palma, T.D. Matola, A. Apicella,, R. Fortuato, R. Zarrilli
and A. M. Acquaviva, Mol. Pharmacol. vol. 64, pp. 407-414, 2003.
[14] J. Kochana, A. Zakrzewska, A. Parczewski, and J. Wilamowski, J. of
Liq. Chro. & Relat. Tech., vol. 28, pp. 2875-2886, 2005.
[15] J. Kochana, A. Parczewski and J. Wilamowski, J. of Liq. Chro. & Relat.
Tech., vol. 29, pp. 1247-1256, 2006.
[1] The Salt Collaborative Group. Lancet., vol. 388, pp. 1345-1349, 1991.
[2] M. Lecomte, O.J.C. Laneuville, D.L. Dewitt, W.L. Smith, J. Biochem.,
vol. 269, pp. 13207-13215, 1994.
[3] J.P. Metraux, H. Signer, J. Ryals, E. Ward, M. Wyss-Benz, G. Gaudin,
K. Raschdorf, E. Schmid, W. Blum, and B. Inverardi, Science, vol. 250,
pp. 1004-1006, 1990.
[4] J. Malamy, J.P. Carr, D.F. Klessig, and I. Raskin, Science, vol. 250, pp.
1002-1004, 1990.
[5] G.A. Higgs, J.A. Salmon, B. Henderson, and J.R. Vane, Proc. Natl.
Acad. Sci.USA., vol. 84, pp. 1417-1420, 1987.
[6] M.J .Thun, M.M. Namboodiri, E.E. Calle, W.D. Flanders, and C.W.
Heath, Cancer Res., vol. 53, pp. 122-1327, 1993.
[7] X.M. Xu, G, Sansores, A. Chenxm, B. Matijevik, N. Aleksic, P. Dum,
and A.Wukk, Proc. Nati. Acad. Sci. USA, vol. 96, pp. 5292-5297, 1999.
[8] S. Dongz, B. Huangc, R.E. Brown, and Z. Mawy, J. Biol. Chem. vol.
271, pp. G 722 -G 727, 1996.
[9] N.H. Pillinger, A. Capodicic, P. Rosenthal, N. Kheterpal, B. Hanfts,
M.R. Philips and G. Welssmann. Pro. Natl. Acad. Sci. USA., vol. 95,
pp. 14540-14545, 1998.
[10] P. Ricchi, S. Pignata, A. Dipoplo, A.M. Memoli, A. Apicellu, R. Zarrilli,
and A.M. Acquaviva, Int. J. cancer, vol. 73, pp. 880-884, 1997.
[11] P. Ricchi, T. Dimatola, A. Ruggieog , D. Zanzi, A. Apicellu, A.
Dipalma, M. Pensabene, S. Pignata, R. Zarrilli, and A.M. Acquaviva,
Brj. Cancer, vol. 86, pp. 1501-1509, 2002.
[12] B. K. Low, M. E. Waltner-Low, A. J. Entingh, A. Chytil, M.E. Akre, P.
Norgaard, and H.L. Moses, J. Biol. Chem., vol. 275, pp. 38261-38267,
2000.
[13] P. Ricchi, A.D. Palma, T.D. Matola, A. Apicella,, R. Fortuato, R. Zarrilli
and A. M. Acquaviva, Mol. Pharmacol. vol. 64, pp. 407-414, 2003.
[14] J. Kochana, A. Zakrzewska, A. Parczewski, and J. Wilamowski, J. of
Liq. Chro. & Relat. Tech., vol. 28, pp. 2875-2886, 2005.
[15] J. Kochana, A. Parczewski and J. Wilamowski, J. of Liq. Chro. & Relat.
Tech., vol. 29, pp. 1247-1256, 2006.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:49663", author = "Elham Moniri and Homayon Ahmad Panahi and Ahmad Izadi and Mohamad Mehdi Parvin and Atyeh Rahimi", title = "Effect of Boric Acid on a-Hydroxy Acids Compounds in Thin Layer Chromatography", abstract = "In this investigation Salicylic acid, Sulfosalicylic acid
and Acetyl salicylic acid were chosen as a sample for thin layer
chromatography (TLC) on silica gel plates. Bicarbonate buffer at
different pH containing different amounts of boric acid was applied
as mobile phase. Specific interaction of these substances with boric
acid has effect on Rf in thin layer chromatography. Regular and
similar trend was observed in variations of Rf for mentioned
compounds in TLC by altering of percentages of boric acid in mobile
phase in pH range of 8-10. Also effect of organic solvent, mixture of
water/ organic solvent and organic solvent containing boric acid as
mobile phase was studied.", keywords = "Thin layer chromatography (TLC), Aspirin,Salicylic acid, Sulfosalycylic acid, Boric acid.", volume = "2", number = "6", pages = "64-3", }