Denitrification of Wastewater Containing High Nitrate Using a Bioreactor System Packed by Microbial Cellulose

A Laboratory-scale packed bed reactor with microbial cellulose as the biofilm carrier was used to investigate the denitrification of high-strength nitrate wastewater with specific emphasis on the effect the nitrogen loading rate and hydraulic retention time. Ethanol was added as a carbon source for denitrification. As a result of this investigation, it was found that up to 500 mg/l feed nitrate concentration the present system is able to produce an effluent with nitrate content below 10 ppm at 3 h hydraulic retention time. The highest observed denitrification rate was 4.57 kg NO3-N/ (m3 .d) at a nitrate load of 5.64 kg NO3- N/(m3 .d), and removal efficiencies higher than 90% were obtained for loads up to 4.2 kg NO3-N/(m3 .d). A mass relation between COD consumed and NO3-N removed around 2.82 was observed. This continuous-flow bioreactor proved an efficient denitrification system with a relatively low retention time.




References:
[1] C. Y. Yang, D. C. Wu, and C. C. Chang, "Nitrate in drinking water and
risk of death from colon cancer in Taiwan," Environ. Int., vol 33, no. 5,
pp. 649-653, Jul. 2007.
[2] D. C. Bouchard, M. K. Williams, and R. Y. Surampalli, "Nitrate
contamination of groundwater: source and potential health effects,"
Journal. AWWA, vol. 84, no 9, pp. 85-90, Sep. 1992.
[3] C. E. Boyd, and C. S. Tucker, "Sustainability and Environmental
Issues," Pond Aquaculture and Water Quality Management, pp. 601-
624, 1998.
[4] S. Aslan, and H. Cakici, "Biological denitrification of drinking water in
a slow sand filter," J. Hazard. Mat., vol. 148, no. 1-2, pp. 253-258, Sep.
2007.
[5] B. Delanghe, F. Nakamura, H. Myoga, Y. Magara, and E. Guibal,
"Drinking water denitrification in a membrane bioreactor," Water. Sci.
Technol., vol. 30, no. 6, pp. 157-160, Jun. 1994.
[6] S. Sozen, and D. Orhon, "The effect of nitrite correction on the
evaluation of the rate of nitrate utilization under anoxic conditions," J.
Chem. Technol. Biotechnol., vol. 74, no. 8, pp. 790-800, Jul. 1999.
[7] I. Kessreu, and Z. Kiss, "Biological denitrification in a continuous-flow
bioreactor containing immobilized Pseudomonas butanovora cells,"
Bioresour. Tech., vol. 87, no. 1, pp. 75-80, March. 2003.
[8] X. Dong, and E. W. Tollner, "Evaluation of Anammox and
denitri¬fication during anaerobic digestion of poultry manure,"
Bioresour. Technol., vol. 86, no. 2, pp. 139-145, Jan. 2003.
[9] R. Pujol, M. Hamon, X. Kendel, and H. Lemmel, "Biofilters: flexible,
reliable biological reactors," Wat. Sci. Tech., vol. 29, no. 10-11, pp. 33-
38, 1994.
[10] W. W. Eckenfelder, and Y. Argaman, Principles of biological and
physical/chemical nitrogen removal, Lewis Publishers, New York, 1991,
pp. 3-42.
[11] V. R. Borregaard, "Experience with nutrient removal in fixed-film
system at full scale wastewater treatment plants, Wat. Sci. Tech., vol.
36, no. 1, pp. 129-137, 1997.
[12] M. Henze, P. Harremoes, J. A. Cour Jansen and E. Arvin, Wastewater
Treatment. "Biological and Chemical Processes (third ed.)," Springer,
Berlin, 2002.
[13] S. Manohar, and T. B. Karegoudar, "Degradation of naphthalene by cells
of Pseudomonas sp. strain NGK 1 immobilized in alginate, agar and
polyacrylamide," Appl. Microbiol. Biotechnol., vol. 49, no. 6, pp. 785-
792, Jun. 1998.
[14] A. Rezaee, J. Drayat, and S. B. Mortazavi, "Removal of mercury from
chlor-alkali industry wastewater using Acetobacter xylinum cellulose,"
Am. J. Environ. Sci.,vol. 1, no. 2, pp. 102-105, 2005.
[15] D. Klemm, D. Schumann, and U. Udhardt, "Bacterial synthesized
cellulose - artificial blood vessels for microsurgery," Prog. Polym.
Sci.,vol. 26, no. 9, pp. 1561-1603, Nov. 2001.
[16] R. Grommen, M. Verhaege, and W. Verstraete, "Removal of nitrate in
aquaria by means of electrochemically generated hydrogen gas as
electron donor for biological denitrification," Aquacult. Eng., vol. 34,
no. 1, pp. 33-39, Jan. 2006.
[17] J. Van Rijn, Y. Tal, and H.J. Schreier, "Denitrification in recirculating
systems: theory and applications," Aquacult. Eng., vol. 34, no. 3, pp.
364-376, May. 2006.
[18] W. Fuchs, G. Schatzmayr, and R. Braun, "Nitrate removal from drinking
water using a membrane-fixed biofilm reactor," Appl. Microbiol.
Biotechnol., vol. 48, no. 2, pp. 267-274, August. 1997.
[19] A. ├ås├©y, H. ├ÿdegaard, K. Bach, R. Pujol, and M. Hamon,
"Denitrification in a packed bed biofilm reactor (BIOFOR)-experiments
with different carbon sources," Water. Res., vol. 32, no. 5, pp. 1463-
1470, May. 1998.
[20] A. Mohseni-Bandpi, and D. J. Elliott, "Groundwater denitrification with
alternative carbon sources," Wat. Sci. Tech., vol. 38, no.6, pp. 237-243,
1998.
[21] S. Hallin, C. F. Lindberg, M. Pell, E. Plaza, and B. Carlsson, "Microbial
adaptation, process performance and a suggested control strategy in a
pre-denitrifying system with ethanol dosage," Wat. Sci. Tech., vol. 34,
no. 1, pp. 91-99, 1996.
[22] S. Hasselblad, and S. Hallin, "Intermittent dosage of ethanol in a predenitrifying
activated sludge process," Wat. Sci. Tech., vol. 34, no 1-2,
pp. 387-389, 1996.
[23] W. J. B. Saliling, P. W. Westerman, and T. M. Losordo, "Wood chips
and wheat straw as alternative biofilter media for denitrification reactors
treating aquaculture and other wastewaters with high nitrate
concentrations," Aquacultur. Eng., vol. 37, no. 3, pp. 222-233, Nov.
2007.
[24] APHA, AWW, WPCF, "Standard Methods for the Examination of
Water and Wastewater," 21th ed, American Public Health Association,
Washington, DC, USA, 2005.
[25] M. Dahab, and Y. W. Lee, "Nitrate removal from water supplies using
biological denitrification," J. Water. Pollut. Control. Fed., vol. 60, no. 9,
pp. 1670-1678, 1998.
[26] A. Mohseni-Bandpi, D. Elliot, and A. Momeny-Mazdeh, "Denitrification
of ground water using acetic acid as a carbon source," Wat. Sci. Tech.,
vol. 40, no. 2, pp. 53-59, 1999.
[27] A. Hirata, Y. Makamura, and S. Tsuneda, "Biological nitrogen removal
from industrial wastewater discharged from metal recovery processes,"
Wat. Sci. Tech., vol. 44, no. 2-3, pp. 171-179, 2001.
[28] Y. Suzuki, T. Maruyama, H. Numata, H. Sato, and M. Asakawa,
"Performance of a closed recirculating system with foam separation,
nitrification and denitrification units," Aquacult. Eng., vol. 29, no. 3-4,
pp. 165-182, December. 2003.
[29] P. Menasveta, T. Panritdam, and P. Sihanonth, "Design and function of a
closed, recirculating seawater system with denitrification for the culture
of black tiger shrimp broodstock," Aquacult. Eng., vol. 25, no. 1, pp.35-
49, Aug. 2001.
[30] Y. K. Kim, K. Nakano, and T. L. Lee, "On-site nitrate removal of
groundwater by an immobilized phychrophilic denitrifier using soluble
starch as a carbon source," J. Biosci. Bioeng., vol. 93, no. 3, pp. 303-
308, Mar. 2002.
[31] Y. Tal, A. Nussinovitch, and J. van Rijn, "Nitrate removal in aquariums
by immobilized denitrifers," Biotechnol. Prog, vol. 19, no. 3, pp. 1019-
1021, 2003.
[32] M. Gomez, J. Gonzalez-Lopez, and E. Honotia-Garcia, "Influence of
carbon source on nitrate removal of contaminated groundwater in a
dentrifying submerged filter," J. Hazard. Mater, vol. 80, no. 1-3, pp. 69-
80, Dec. 2000.
[33] E. J. Park, J. K. Seo, M. R. Kim, I. H. Jung, and J. Y. Kim, "Salinity
acclimation of immobilized freshwater denitrifiers," Aquacult. Eng., vol.
24, no. 3, pp. 169-180, April. 2001.
[34] P. Kesseru, I. Kiss, Z. Bihari, and B. Polyák, "Investigation of the
denitrification activity of immobilized Pseudomonas butanovora cells in
the presence of different organic substrate," Water. Res., vol. 36, no. 6,
pp. 1565-1571, Mar. 2002.
[35] USEPA (US Environmental Protection Agency), "Manual: Nitrogen
Control," EPA/625/R-93/010. Environmental Protection Agency,
Washington, DC., 1993
[36] Metcalf and Eddy Inc, (Tchobanoglous, G, Burton, FL, and Stensel, HD,
Editors), "Wastewater Engineering-Treatment, Disposal and Reuse
(fourth ed.)," McGraw-Hill, New York., 2002.
[37] W. D. Robertson, G. I. Ford and P. S. Lombardo "Wood-based filter for
nitrate removal in septic systems," Trans. ASAE., vol. 48, no. 1, pp.
121-128, 2005.