Antibacterial Activity of Ethanol Extract from Some Thai Medicinal Plants against Campylobacter Jejuni
In this study, the forty Thai medicinal plants were
used to screen the antibacterial activity against Campylobacter jejuni.
Crude 95% ethanolic extracts of each plant were prepared.
Antibacterial activity was investigated by the disc diffusion assay,
and MICs and MBCs were determined by broth microdilution. The
results of antibacterial screening showed that five plants have activity
against C.jejuni including Adenanthera pavonina L., Moringa
oleifera Lam., Annona squamosa L., Hibiscus sabdariffa L. and
Eupotorium odortum L. The extraction of A. pavonina L. and A.
squamosa L. produced an outstanding against C. jejuni, inhibiting
growth at 62.5-125 and 250-500 μg/mL, respectively. The MBCs of
two extracts were just 4-fold higher than MICs against C. jejuni,
suggesting the extracts are bactericidal against this species. These
results indicate that A. pavonina and A. squamosa could potentially
be used in modern applications aimed at treatment or prevention of
foodborne disease from C. jejuni.
[1] Murray PR, Rosenthal KS, Pfaller MA. Medical Microbiology. 6th
edition. Philadelphia: Elsevier Mosby; 2009.
[2] Friedman C, Neimann J, Wegener H, Tauxe R. Epidemiology of
Campylobacter jejuni Infections in the United States and Other
Industrialized Nations Campylobacter. 2 ed: ASM Press; 2000: p. 121 -
38.
[3] Näther G, Alter T, Martin A, Ellerbroek L. Analysis of Risk Factors for
Campylobacter Species Infection in Broiler Flocks. Poult Sci. 2009 June
1, 2009;88(6): 1299-305.
[4] Figueroa G, Troncoso M, Lopez C, Rivas P, Toro M. Occurrence and
Enumeration of Campylobacter spp. During the Processing of Chilean
Broilers. BMC Microbiology. 2009;9(1): 94.
[5] Habib I, Sampers I, Uyttendaele M, Berkvens D, De Zutter L. Baseline
data from a Belgium-wide survey of Campylobacter species
contamination in chicken meat preparations and considerations for a
reliable monitoring program. Appl Environ Microb. 2008 September 1,
2008;74(17): 5483-9.
[6] Mor-Mur M, Yuste J. Emerging Bacterial Pathogens in Meat and
Poultry: an Overview. Food and Bioprocess Tech. 2010;3(1): 24-35.
[7] Centers for Disease Control and Prevention. Outbreak of Campylobacter
jejuni Infections Associated with Drinking Unpasteurized Milk
Produced Through a Cow-Leasing Program---Wisconsin, 2001. MMWR
weekly. 2002 June, 28;Sect. 548-9.
[8] Lee C-F, Han C-K, Tsau J-L. In vitro inhibitory activity of Chinese leek
extract against Campylobacter species. Int J Food Microbiol.
2004;94(2): 169-74.
[9] Ramadan MF, Selim Asker MM. Antimicrobical and antivirial impact of
novel quercetin-enriched lecithin. J Food Biochem. 2009;33(4): 557-71.
[10] Rauha J-P, Remes S, Heinonen M, Hopia A, K├ñhkönen M, Kujala T, et
al. Antimicrobial effects of Finnish plant extracts containing flavonoids
and other phenolic compounds. Int J Food Microbiol. 2000;56(1): 3-12.
[11] Kaur GJ, Arora DS. Antibacterial and Phytochemical Screening of
Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi.
BMC complementary and alternative medicine. 2009 August, 6: 1-10.
[12] Ríos JL, Recio MC. Medicinal Plants and Antimicrobial Activity. J
Ethnopharmacol. 2005;100(1-2): 80-4.
[13] Ballabh B, Chaurasia OP. Traditional Medicinal Plants of Cold Desert
Ladakh--Used in Treatment of Cold, Cough and Fever. J
Ethnopharmacol. 2007;112(2): 341-9.
[14] Grabley S, Thiericke R. Drug discovery from nature. Berlin: Springer-
Verlag; 2000.
[15] Thailand Public Relations Department. Thailand Declares 2010 the Year
of Diodiversity. Thailand: Thai Government; 2009 December, 22.
[16] Craig W. Ginger - Useful for More Than Just Candy. Vegetarianism &
Vegetarian Nutrition 2003.
[17] Saralamp P, Chukul W, Temsiririrkkul R, Clayton T. Medicinal Plants in
Thailand Volume I. Bangkok: Faculty of Pharmacy, Mahidol University;
1996.
[18] Ratnam KV, Raju RRV. In vitro Antimicrobial Screening of the Fruit
Extracts of two Syzygium Species (Myrtaceae). Advances in Biological
Research. 2008;2(1-2): 17-20.
[19] Philip K, Malek SNA, Sani W, Shin SK, Kumar S, Lai HS, et al.
Antimicrobial activity of some medicinal plants from Malaysia. Am J
Appl Sci. 2009;6(8): 1613-7.
[20] CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for
Bacteria That Grow Aerobically: Approved Guideline (M7-M5). Wayne,
Pennsylvania: Clinical and Laboratory Standards Institute; 2000.
[21] CLSI. Methods for Determining Bactericidal Activity of Antimicrobial
Agents: Approved Guideline (M26-A). Wayne, Pennsylvania: Clinical
and Laboratory Standards Institute; 1999.
[22] Cowan MM. Plant Products as Antimicrobial Agents. Clin Microbiol
Rev. 1999 October 1, 1999;12(4): 564-82.
[23] Al-Bayati F, Al-Mola H. Antibacterial and antifungal activities of
different parts of Tribulus terrestris L. growing in Iraq. Journal of
Zhejiang University - Science B. 2008;9(2): 154-9.
[24] Ncube NS, Afolayan AJ, Okoh AI. Assessment Techniques of
Antimicrobial Properties of Natural Compounds of Plant Origin: Current
Methods and Future Trends. Afr J Biotechnol. 2008;7(14): 1797-806.
[25] Nostro Aea. Extraction Methods and Bioautography for Evaluation of
Medicinal Plant Antimicrobial Activity. Lett Appl Microbiol. 2000;30:
379-84.
[26] Adedapo ADA, Osude YO, Adedapo AA. Blood Pressure Lowering
Effect of Adenanthera pavonina Seed Extract on Normotensive Rats
Aduragbenro. Records of Natural Products. 2009;3(2): 82-9.
[27] Ara A, Saleh-e-In MM, Ahmed NU, Ahmed M, Hashem A, Bachar SC.
Phytochemical Screening, Analgesic, Antimicrobial and Anti-Oxidant
Activities of Bark Extracts of Adenanthera pavonina L. (Fabaceae). Adv
Nat Appl Sci. 2010;4(3): 352-60.
[28] Prescott LM, Harley JP, Klein DA. Microbiology. 6th edition. New
York: Mc Graw-Hill; 2005.
[1] Murray PR, Rosenthal KS, Pfaller MA. Medical Microbiology. 6th
edition. Philadelphia: Elsevier Mosby; 2009.
[2] Friedman C, Neimann J, Wegener H, Tauxe R. Epidemiology of
Campylobacter jejuni Infections in the United States and Other
Industrialized Nations Campylobacter. 2 ed: ASM Press; 2000: p. 121 -
38.
[3] Näther G, Alter T, Martin A, Ellerbroek L. Analysis of Risk Factors for
Campylobacter Species Infection in Broiler Flocks. Poult Sci. 2009 June
1, 2009;88(6): 1299-305.
[4] Figueroa G, Troncoso M, Lopez C, Rivas P, Toro M. Occurrence and
Enumeration of Campylobacter spp. During the Processing of Chilean
Broilers. BMC Microbiology. 2009;9(1): 94.
[5] Habib I, Sampers I, Uyttendaele M, Berkvens D, De Zutter L. Baseline
data from a Belgium-wide survey of Campylobacter species
contamination in chicken meat preparations and considerations for a
reliable monitoring program. Appl Environ Microb. 2008 September 1,
2008;74(17): 5483-9.
[6] Mor-Mur M, Yuste J. Emerging Bacterial Pathogens in Meat and
Poultry: an Overview. Food and Bioprocess Tech. 2010;3(1): 24-35.
[7] Centers for Disease Control and Prevention. Outbreak of Campylobacter
jejuni Infections Associated with Drinking Unpasteurized Milk
Produced Through a Cow-Leasing Program---Wisconsin, 2001. MMWR
weekly. 2002 June, 28;Sect. 548-9.
[8] Lee C-F, Han C-K, Tsau J-L. In vitro inhibitory activity of Chinese leek
extract against Campylobacter species. Int J Food Microbiol.
2004;94(2): 169-74.
[9] Ramadan MF, Selim Asker MM. Antimicrobical and antivirial impact of
novel quercetin-enriched lecithin. J Food Biochem. 2009;33(4): 557-71.
[10] Rauha J-P, Remes S, Heinonen M, Hopia A, K├ñhkönen M, Kujala T, et
al. Antimicrobial effects of Finnish plant extracts containing flavonoids
and other phenolic compounds. Int J Food Microbiol. 2000;56(1): 3-12.
[11] Kaur GJ, Arora DS. Antibacterial and Phytochemical Screening of
Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi.
BMC complementary and alternative medicine. 2009 August, 6: 1-10.
[12] Ríos JL, Recio MC. Medicinal Plants and Antimicrobial Activity. J
Ethnopharmacol. 2005;100(1-2): 80-4.
[13] Ballabh B, Chaurasia OP. Traditional Medicinal Plants of Cold Desert
Ladakh--Used in Treatment of Cold, Cough and Fever. J
Ethnopharmacol. 2007;112(2): 341-9.
[14] Grabley S, Thiericke R. Drug discovery from nature. Berlin: Springer-
Verlag; 2000.
[15] Thailand Public Relations Department. Thailand Declares 2010 the Year
of Diodiversity. Thailand: Thai Government; 2009 December, 22.
[16] Craig W. Ginger - Useful for More Than Just Candy. Vegetarianism &
Vegetarian Nutrition 2003.
[17] Saralamp P, Chukul W, Temsiririrkkul R, Clayton T. Medicinal Plants in
Thailand Volume I. Bangkok: Faculty of Pharmacy, Mahidol University;
1996.
[18] Ratnam KV, Raju RRV. In vitro Antimicrobial Screening of the Fruit
Extracts of two Syzygium Species (Myrtaceae). Advances in Biological
Research. 2008;2(1-2): 17-20.
[19] Philip K, Malek SNA, Sani W, Shin SK, Kumar S, Lai HS, et al.
Antimicrobial activity of some medicinal plants from Malaysia. Am J
Appl Sci. 2009;6(8): 1613-7.
[20] CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for
Bacteria That Grow Aerobically: Approved Guideline (M7-M5). Wayne,
Pennsylvania: Clinical and Laboratory Standards Institute; 2000.
[21] CLSI. Methods for Determining Bactericidal Activity of Antimicrobial
Agents: Approved Guideline (M26-A). Wayne, Pennsylvania: Clinical
and Laboratory Standards Institute; 1999.
[22] Cowan MM. Plant Products as Antimicrobial Agents. Clin Microbiol
Rev. 1999 October 1, 1999;12(4): 564-82.
[23] Al-Bayati F, Al-Mola H. Antibacterial and antifungal activities of
different parts of Tribulus terrestris L. growing in Iraq. Journal of
Zhejiang University - Science B. 2008;9(2): 154-9.
[24] Ncube NS, Afolayan AJ, Okoh AI. Assessment Techniques of
Antimicrobial Properties of Natural Compounds of Plant Origin: Current
Methods and Future Trends. Afr J Biotechnol. 2008;7(14): 1797-806.
[25] Nostro Aea. Extraction Methods and Bioautography for Evaluation of
Medicinal Plant Antimicrobial Activity. Lett Appl Microbiol. 2000;30:
379-84.
[26] Adedapo ADA, Osude YO, Adedapo AA. Blood Pressure Lowering
Effect of Adenanthera pavonina Seed Extract on Normotensive Rats
Aduragbenro. Records of Natural Products. 2009;3(2): 82-9.
[27] Ara A, Saleh-e-In MM, Ahmed NU, Ahmed M, Hashem A, Bachar SC.
Phytochemical Screening, Analgesic, Antimicrobial and Anti-Oxidant
Activities of Bark Extracts of Adenanthera pavonina L. (Fabaceae). Adv
Nat Appl Sci. 2010;4(3): 352-60.
[28] Prescott LM, Harley JP, Klein DA. Microbiology. 6th edition. New
York: Mc Graw-Hill; 2005.
@article{"International Journal of Medical, Medicine and Health Sciences:50821", author = "Achara Dholvitayakhun and Nathanon Trachoo", title = "Antibacterial Activity of Ethanol Extract from Some Thai Medicinal Plants against Campylobacter Jejuni", abstract = "In this study, the forty Thai medicinal plants were
used to screen the antibacterial activity against Campylobacter jejuni.
Crude 95% ethanolic extracts of each plant were prepared.
Antibacterial activity was investigated by the disc diffusion assay,
and MICs and MBCs were determined by broth microdilution. The
results of antibacterial screening showed that five plants have activity
against C.jejuni including Adenanthera pavonina L., Moringa
oleifera Lam., Annona squamosa L., Hibiscus sabdariffa L. and
Eupotorium odortum L. The extraction of A. pavonina L. and A.
squamosa L. produced an outstanding against C. jejuni, inhibiting
growth at 62.5-125 and 250-500 μg/mL, respectively. The MBCs of
two extracts were just 4-fold higher than MICs against C. jejuni,
suggesting the extracts are bactericidal against this species. These
results indicate that A. pavonina and A. squamosa could potentially
be used in modern applications aimed at treatment or prevention of
foodborne disease from C. jejuni.", keywords = "Antibacterial activity, Thai medicinal plants,
Campylobacter jejuni", volume = "6", number = "5", pages = "125-4", }
