Analysis and Remediation of Fecal Coliform Bacteria Pollution in Selected Surface Water Bodies of Enugu State of Nigeria

The assessment of surface waters in Enugu metropolis for fecal coliform bacteria was undertaken. Enugu urban was divided into three areas (A1, A2 and A3), and fecal coliform bacteria analysed in the surface waters found in these areas for four years (2005-2008). The plate count method was used for the analyses. Data generated were subjected to statistical tests involving; Normality test, Homogeneity of variance test, correlation test, and tolerance limit test. The influence of seasonality and pollution trends were investigated using time series plots. Results from the tolerance limit test at 95% coverage with 95% confidence, and with respect to EU maximum permissible concentration show that the three areas suffer from fecal coliform pollution. To this end, remediation procedure involving the use of saw-dust extracts from three woods namely; Chlorophora-Excelsa (C-Excelsa),Khayan-Senegalensis,(CSenegalensis) and Erythrophylum-Ivorensis (E-Ivorensis) in controlling the coliforms was studied. Results show that mixture of the acetone extracts of the woods show the most effective antibacterial inhibitory activities (26.00mm zone of inhibition) against E-coli. Methanol extract mixture of the three woods gave best inhibitory activity (26.00mm zone of inhibition) against S-areus, and 25.00mm zones of inhibition against E-Aerogenes. The aqueous extracts mixture gave acceptable zones of inhibitions against the three bacteria organisms.




References:
[1] G. Kiely, "Environmental Engineering International edition", MaGraw
Hill, New York, 1999, PP 52-146.
[2] H. Ezeigbo and B. Ezeanyim,. (1993), Mine Water and Environment;
12, 53-62, 1993.
[3] R. P. Lettenmaier, (1991), Trends in Stream quality in continental
United States, 1978-1987, Water Resour Res., 27 327-339.
[4] T. A Cohn and L. L.Delong, "Estimating Constitutent Loads". Water
Resour. Res 25(5), 937-942. 1989.
[5] C. M. Ademoroti "Standard Methods fro Water and effluents Analysis",
Foludex Press Ltd. Ibadan Nigeria,2002, PP 2-44.
[6] S. A Junaid, A O. Olabode, F. C Onwuliri and A. E. Okworiu, "The
antimicrobial properties of Ocimum gratissimum extracts on some
sleected bacterial gastrointestinal Isolates". Afr. J. Biotechnol. 5(22):
2315-2321. 2006.
[7] J H Douyghari, "Antimicrobial Activity of Tamarinchus Indica Linn
Tropical" J. Pharm. Res., 5(2); 592-603, 2006.
[8] H.J.Boer and A. Kool, "Antifungal and antibacterial activity of some
Herbal remedies from Tanzarica" J.Ethopharm; 96:461-469, 2005.
[9] B. Bibitaa,"Antibacterial activity of different plant extracts", Indian
Journal of Microbiol, 42: 361-363,2002
[10] P. Aidan, V. Rose, F. Blamea, A. Tomas, and C Salvador, "Paraguyam
Plants Used in Traditional Medicine", J. Ethnopharm; 16, 93-98, 2001.
[11] J. H Doughari, A. M. Elmanhmood, and S.Manzara, "Studies on the
antibacterial activity of root extracts of Carica papaya," Afr. J.
Microbio. Res. 9(2), 37-41, 2007
[12] O. Ogunlaja, and A. Ogunlaja, "Physicochemical analysis of water
Sources in Ubeji Community and their Histological Impacts on Organs
of Albino Mice". Journal of Applied Sciences Environmental
Management, 11(4): 91 - 94, 2007.
[13] G. C. William, "Understanding Microbes" W.H. Freeman and Coy.
USA. 2000.
[14] C. Erin, and D. E. Mark, "A Statistical Examination of Water quality in
two-Towa lakes", American journal of undergraduate Research, 1(2),
502-530,2002
[15] A. M. Libetran, "Water quality sampling, some statistical
considerations", Water Resour. Res; 15, 1717-1728, 1979.
[16] J. T. Spence, J. W. Cotton, B. J. Underwood, "Elementary Statistics 3rd
Ed".; Prentice Hlal Inc. New Jersey, 1974.
[17] R. L. Ott "An Introduction to Statistical Methods and data analysis",
Duxburg Press, Belont, California, P 1,051, 1993
[18] P.Y. Idakwo, and G. O. Abu, "Distribution and Statistical Analysis of
Bacteria in Lake Alau in the Arid Northern Nigeria", J. Appl Sci.
Environ. Mgt; 8(1), 5-9, 2004
[19] V. G. Christensen, P. P. Rasmussen, and A. C. Ziegler "Realtime Water-
Quality Monitoring and Regression Analysis to Estimate Nutrient and
Bacteria Concentration in Kansas Stream", USGS, 4821, KS66-049,
USA, 2008.
[20] C. R. Andronachie, and Q. Xiang, "Statistical Analysis of Surface
Water Quality Data of Eastern Massachusetts", Geological Society of
America-Philadelphia Annual Meeting, 38(7), 438, 2006.
[21] R. Brender, and O. Matt, "Statistical Analysis of Streambed Sedimnent
Grain Size Distribution", Redwood Sci. Symposium Califonia, 2004.
[22] N. Nishida, "Statistical Analysis of water Quality Data of the Oota River
system", Bulletin of Faculty of Human Life and Environmental
Sciences, 8, 13-25. 2002.
[23] S.P. Latha and K. Kannabiran, "Antimicrobial activity and
Phytochemicals of Solannum Trinobatum Linn", Afr. J. Biotechnol
5(23); 2402-2404, 2006
[24] A. C. Emeruwa, "Antibacterial Substance from arica Papaya Fruit
Extract", J. Natl. Product; 3; 123-127,1992.
[25] P. Jigna,, K. Nehal,, C. Sumitra,"Evlauation of antibacterial and
Phytochemical analysis of Bauhinia variegate L. bark"; Afr. J. Biomed
Res; 9(1): 53-56, 2006
[26] W. B. Bowden "Comparison of two direct count techniques for
enumerating aquatic Bacteria". Appl. Environ, Microbiol, 13; 1229-
1232, 1977.
[27] C. J. Pretorins, and E. Watts, "Purification and Identification of active
components of Carpobrotus edulis", J. Ethnopharm, 76:87-91, 2001
[28] D. Srinivasan, L. P. Perumalsamy and S. T. Nathen, "Antimicrobial
activity of certain Indian Medicinal plants used in folkoric Medicine", J.
Ethropharm, 94,2001.
[29] B. Z. Diamant, "Environment Health Impacts of Water use in Africa",
Proc. Water Techn. 13; 171-178, 1980.