A Study of the Cyclic Variations of the Enzyme and the Electrolyte Activity in Uterine and Oviducal Secretions during an Estrous Cycle of the Ewe

Uterine and oviducal fluids are necessary for capacitation of the spermatozoa and early embryonic development. The aim of the present study was to determine the effects of estrous cycle phases (follicular and luteal) on some biological parameters (enzymes, electrolytes and total proteins) in uterine and oviducal secretions of ewes. Oviducal and uterine fluids were collected, diluted and centrifuged. According to our results, concentrations of GPT, G6PDH, total proteins, K and Na were significantly (P <0.05) higher at the luteal phase, however, the levels of aldolase, Mg, Ca and P were significantly (P <0.05) higher at the follicular phase in uterine secretions. While, only oviducal K and Ca were significantly (P<0.05) higher at the follicular phase. Our study revealed the existence of significant cyclic variations for some uterine and oviducal parameters which indicates the effect of ovarian hormones on the components of genital secretions.




References:
[1] S. Hugentobler, D. Morris, J. Sreenan and M. Diskin, "Ion
concentrations in oviduct and uterine fluid and blood serum during the
estrous cycle in the bovine," Theriogenology, vol. 68, pp. 538-548,
2007.
[2] P. Lamothe , P. Guay, M. Ibrahim and A. Tremblay A,"Total proteins
and their fractions in the serum and endometrial secretions in the cow
with of sine materia infertility, "Can. J. comp. Med, vol. 36, pp.94-98,
August. 1972.
[3] C. Thibault and M-C. Levasseur, " Reproduction in mammals and
human," Nouv. Éd. ent. ref. ed. Paris: INRA : Ellipses, 2001:928 p., 928
p. de pl.2001.
[4] P. Guay and P. Lamothe, "Biochemical study of genital secretions of
infertile bovine females with normal estrous cycles," Can. J. comp. Med,
Vol. 33, pp.33-93-97, 1969.
[5] G.P. Roberts, J.M. Parker and H.W. Symonds,"Macromolecular
components of genital tract fluids from the sheep,"J.Reprod.Fert,
vol.107, pp.48-99, 1976.
[6] J.L. Perkins, L. Goode, W.A. Wilder and D.B. Henson,"Collection of
Secretions from the Oviduct and Uterus of the Ewe," J Anim Sci, vol. 24,
pp.383-387, 1965.
[7] M. L. Fahning, R. H. SChultz and E. F. Graham, "The free amino acid
content of uterine fluids and blood serum in the cow," J. Reprod. Fert,
vol.13, pp. 229-236, 1967.
[8] S.M. Alavi-Shoushtari, S. Asri-Rezai and J. Abshenas, "A study of the
uterine protein variations during the estrous cycle in the cow: Molecular
weights determination.Anim. Reprod," Science, vol. 105, pp. 302-310,
2006.
[9] D. Olds and N L. Vandemark, "Composition of luminal fluids of bovine
female genitalia," Fertility Sterility, vol. 8, pp.345-354, 1957.
[10] M. L. Fahning, R. H. SChultz and E. F. Graham, "The free amino acid
content of uterine fluids and blood serum in the cow," J. Reprod. Fert,
vol.13, pp. 229-236, 1967.
[11] S.M. Alavi-Shoushtari, S. Asri-Rezai and J. Abshenas, "A study of the
uterine protein variations during the estrous cycle in the cow: Molecular
weights determination.Anim. Reprod," Science, vol. 105, pp. 302-310,
2006.