A New Method to Enhance Contrast of Electron Micrograph of Rat Tissues Sections
This report presents an alternative technique of
application of contrast agent in vivo, i.e. before sampling. By this
new method the electron micrograph of tissue sections have an
acceptable contrast compared to other methods and present no artifact
of precipitation on sections. Another advantage is that a small amount
of contrast is needed to get a good result given that most of them are
expensive and extremely toxic.
[1] R. A. Bloodgood, “The history of medical histology teaching: where
have we come from and where are we going?” FASEB J., vol. 27, pp
191-1, 2013.
[2] J. A. Hightower, F.R. Boockfor, C. A.Blake, and C. F. Millette, “The
Standard Medical Microscopic Anatomy Cours,” Histology Circa, The
anatomical record (New Anat.), vol. 257, pp. 96–101, 1999.
[3] E. M. Slayter and H S. Slayte, “Light and Electron Microscopy”,
Cambridge University Press, Cambridge, pp 100-110.
[4] R.M., Amderson and D, Walck, “Specimen Preparation for
Transmission Electron Microscopy IV” in MRS Proceedings, Jun 5,
2014, pp. 480-483.
[5] M. A. Hayat, “Basic Techniques for Transmission Electron
Microscopy,” 1986.
[6] J. Kuo, Electron microscopy: methods and protocols. 2 ed. Totowa, N.J.:
Humana Press, 2007, pp. 608-624.
[7] I. M. Watt, “The Principles and Practice of Electron Microscopy,”
Cambridge University Press, 1997.
[8] J. Fertig and H. Rose, “On the theory of image formation in the electron
microscope,” Optik, vol. 54, pp.165- 174, 1979.
[9] L. E. Ross, M Dykstra, “Biological Electron Microscopy: Theory,
techniques and troubleshooting” Springer, New York. , 2003.
[10] M. Nakakoshi, H. Nishioka, and E. Katayama, “New versatile staining
reagents for biological transmission electron microscopy that substitute
for uranyl acetate,” J Electron Microsc., vol. 60, pp. 401-407, 2011.
[11] S. W. Avery, “Routine Transmission Electron Microscopy (TEM)
Staining Protocol for Tissues,” in Transmission Electron Microscopy,
2nd ed. vol. 3, J. Peters, Ed. New York: McGraw-Hill, 1964, pp. 15–64
[12] S. W. Avery, E. A. Ellis, “Methods for removing uranyl acetate
precipitate from ultrathin sections,” Stain Technol., vol. 53, no. 3, pp.
137-40, 1978.
[13] E. S. Reynolds, “The use of lead citrate at high pH as an electron-opaque
stain in electron microscopy,” Journal of Cellular Biology, vol. 17, pp
208-214, 1963.
[14] H. Niederstrasser, “Electron microscopy, Negative staining”, Snaggled
Works, 2004 http://www.snaggledworks.com
[15] G. J. Gage, D. R. Kipke, and W. Shain, “Whole Animal Perfusion
Fixation for Rodents,” Journal of Visualized Experiments (JoVE), vol
65, pp. 3564, 2012.
[16] R. Kasukurthi, M.J. Brenner, A.M. Morre, A. Moradzadeh, Z.R. Wilson,
K.B. Santosa, S.E. Mackinnon, and D. A. Hunter, “Transcardial
perfusion versus immersion fixation for assessment of peripheral nerve
regeneration,” Journal of Neurosciences Methods, vol. 184, pp. 303-309,
2009.
[17] M. L. Watson, “Staining of Tissue Sections for Electron Microscopy
with Heavy Metals,” Journal of Biophysical and Biochemical Biology,
vol. 4, no. 4, pp. 475-478, 1958.
[18] R. S. P. Bizerra, G. B. Costa, L. P. Labejof, “Estudo morfológico em
microscopia de luz e eletrônica da mucosa intestinal de ratos após
administração de acetato de uranilo,” in Abstract Book of the 16°
Seminário de Iniciação Cientifica, Universidade Estadual de Santa Cruz,
Cruz , Ilhéus, Bahia, 2010, pp. 141-141.
[19] T. B. dos Santos, L. P. Labéjof, “Estudo correlativo em microscopia de
luz e eletrônica do modo de acumulação do urânio no enterócito após
ingestão experimental,”in Abstract Book of the 18° Seminário de
Iniciação Cientifica , Universidade Estadual de Santa Cruz, Cruz ,
Ilhéus, Bahia, 2012, pp. 765-765.
[20] F. Paquet, “Accumulation and distribution of uranium in rats after
chronic exposure by ingestion,” Health Physics, vol. 90, no. 2, pp.139-
147, 2006.
[21] I. Dublineau, “Absorption of uranium through the entire gastrointestinal
tract of the rat,” Internat. J. Rad. Biol., vol. 81, no. 6, pp. 473–482,
2005.
[22] I. Dublineau, “Absorption, accumulation and biological effects of
depleted uranium in Peyer’s patches of rats,” Toxicology, vol. 227, pp.
227–239, 2006.
[23] W. Rasband, “ImageJ - Image processing and Analysis in Java, National
Institute of Mental Health (NIMH) of the National Institutes of Health
(NIH), http://rsb.info.nih.gov/ij/.
[24] P. Bankhead. Analyzing fluorescence microscopy images with ImageJ
Queen's University Belfast 2014.
[25] F. Zernike, “How I discovered phase contrast” Nobel Lecture, December
11, 1953
[1] R. A. Bloodgood, “The history of medical histology teaching: where
have we come from and where are we going?” FASEB J., vol. 27, pp
191-1, 2013.
[2] J. A. Hightower, F.R. Boockfor, C. A.Blake, and C. F. Millette, “The
Standard Medical Microscopic Anatomy Cours,” Histology Circa, The
anatomical record (New Anat.), vol. 257, pp. 96–101, 1999.
[3] E. M. Slayter and H S. Slayte, “Light and Electron Microscopy”,
Cambridge University Press, Cambridge, pp 100-110.
[4] R.M., Amderson and D, Walck, “Specimen Preparation for
Transmission Electron Microscopy IV” in MRS Proceedings, Jun 5,
2014, pp. 480-483.
[5] M. A. Hayat, “Basic Techniques for Transmission Electron
Microscopy,” 1986.
[6] J. Kuo, Electron microscopy: methods and protocols. 2 ed. Totowa, N.J.:
Humana Press, 2007, pp. 608-624.
[7] I. M. Watt, “The Principles and Practice of Electron Microscopy,”
Cambridge University Press, 1997.
[8] J. Fertig and H. Rose, “On the theory of image formation in the electron
microscope,” Optik, vol. 54, pp.165- 174, 1979.
[9] L. E. Ross, M Dykstra, “Biological Electron Microscopy: Theory,
techniques and troubleshooting” Springer, New York. , 2003.
[10] M. Nakakoshi, H. Nishioka, and E. Katayama, “New versatile staining
reagents for biological transmission electron microscopy that substitute
for uranyl acetate,” J Electron Microsc., vol. 60, pp. 401-407, 2011.
[11] S. W. Avery, “Routine Transmission Electron Microscopy (TEM)
Staining Protocol for Tissues,” in Transmission Electron Microscopy,
2nd ed. vol. 3, J. Peters, Ed. New York: McGraw-Hill, 1964, pp. 15–64
[12] S. W. Avery, E. A. Ellis, “Methods for removing uranyl acetate
precipitate from ultrathin sections,” Stain Technol., vol. 53, no. 3, pp.
137-40, 1978.
[13] E. S. Reynolds, “The use of lead citrate at high pH as an electron-opaque
stain in electron microscopy,” Journal of Cellular Biology, vol. 17, pp
208-214, 1963.
[14] H. Niederstrasser, “Electron microscopy, Negative staining”, Snaggled
Works, 2004 http://www.snaggledworks.com
[15] G. J. Gage, D. R. Kipke, and W. Shain, “Whole Animal Perfusion
Fixation for Rodents,” Journal of Visualized Experiments (JoVE), vol
65, pp. 3564, 2012.
[16] R. Kasukurthi, M.J. Brenner, A.M. Morre, A. Moradzadeh, Z.R. Wilson,
K.B. Santosa, S.E. Mackinnon, and D. A. Hunter, “Transcardial
perfusion versus immersion fixation for assessment of peripheral nerve
regeneration,” Journal of Neurosciences Methods, vol. 184, pp. 303-309,
2009.
[17] M. L. Watson, “Staining of Tissue Sections for Electron Microscopy
with Heavy Metals,” Journal of Biophysical and Biochemical Biology,
vol. 4, no. 4, pp. 475-478, 1958.
[18] R. S. P. Bizerra, G. B. Costa, L. P. Labejof, “Estudo morfológico em
microscopia de luz e eletrônica da mucosa intestinal de ratos após
administração de acetato de uranilo,” in Abstract Book of the 16°
Seminário de Iniciação Cientifica, Universidade Estadual de Santa Cruz,
Cruz , Ilhéus, Bahia, 2010, pp. 141-141.
[19] T. B. dos Santos, L. P. Labéjof, “Estudo correlativo em microscopia de
luz e eletrônica do modo de acumulação do urânio no enterócito após
ingestão experimental,”in Abstract Book of the 18° Seminário de
Iniciação Cientifica , Universidade Estadual de Santa Cruz, Cruz ,
Ilhéus, Bahia, 2012, pp. 765-765.
[20] F. Paquet, “Accumulation and distribution of uranium in rats after
chronic exposure by ingestion,” Health Physics, vol. 90, no. 2, pp.139-
147, 2006.
[21] I. Dublineau, “Absorption of uranium through the entire gastrointestinal
tract of the rat,” Internat. J. Rad. Biol., vol. 81, no. 6, pp. 473–482,
2005.
[22] I. Dublineau, “Absorption, accumulation and biological effects of
depleted uranium in Peyer’s patches of rats,” Toxicology, vol. 227, pp.
227–239, 2006.
[23] W. Rasband, “ImageJ - Image processing and Analysis in Java, National
Institute of Mental Health (NIMH) of the National Institutes of Health
(NIH), http://rsb.info.nih.gov/ij/.
[24] P. Bankhead. Analyzing fluorescence microscopy images with ImageJ
Queen's University Belfast 2014.
[25] F. Zernike, “How I discovered phase contrast” Nobel Lecture, December
11, 1953
@article{"International Journal of Biological, Life and Agricultural Sciences:70720", author = "Lise P. Labéjof and Raiza S. P. Bizerra and Galileu B. Costa and Thaísa B. dos Santos", title = "A New Method to Enhance Contrast of Electron Micrograph of Rat Tissues Sections", abstract = "This report presents an alternative technique of
application of contrast agent in vivo, i.e. before sampling. By this
new method the electron micrograph of tissue sections have an
acceptable contrast compared to other methods and present no artifact
of precipitation on sections. Another advantage is that a small amount
of contrast is needed to get a good result given that most of them are
expensive and extremely toxic.", keywords = "Image quality, Microscopy research, Staining
technique, Ultrathin section.", volume = "9", number = "6", pages = "694-5", }
