Abstract: The objective of the present study was to determine the polymorphism in the leptin (332G>A) and its association with biometric traits in Sanjabi sheep. For this purpose, blood samples from 96 rams were taken, and tail length, width tail, circumference tail, body length, body width, and height were simultaneously recorded. PCR was performed using specific primer to amplify 463 bp fragment including exon 3 of leptin gene, and PCR products were digested by Cail restriction enzymes. The 332G>A (at 332th nucleotide of exon 3 leptin gene) that caused an amino acid change from Arg to Gln was detected by Cail (CAGNNNCTG) endonuclease, as the endonuclease cannot cut this region if G nucleotide is located in this position. Three genotypes including GG (463), GA (463, 360and 103 bp) and GG (360 bp and 103 bp) were identified after digestion by enzyme. The estimated frequencies of three genotypes including GG, GA, and AA for 332G>A locus were 0.68, 0.29 and 0.03 and those were 0.18 and 0.82 for A and G alleles, respectively. In the current study, chi-square test indicated that 332G>A positions did not deviate from the Hardy–Weinberg (HW) equilibrium. The most important reason to show HW equation was that samples used in this study belong to three large local herds with a traditional breeding system having random mating and without selection. Shannon index amount was calculated which represent an average genetic variation in Sanjabi rams. Also, heterozygosity estimated by Nei index indicated that genetic diversity of mutation in the leptin gene is moderate. Leptin gene polymorphism in the 332G>A had significant effect on body length (P0.05). This non-synonymous SNP resulted in different amino acid changes at codon positions111(R/Q). As leptin activity is localized, at least in part, in domains between amino acid residues 106-1406, it is speculated that the detected SNP at position 332 may affect the activity of leptin and may lead to different biological functions. Based to our results, due to significant effect of leptin gene polymorphism on body size traits, this gene may be used a candidate gene for improving these traits.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: One of the purposes of the robust method of
estimation is to reduce the influence of outliers in the data, on the
estimates. The outliers arise from gross errors or contamination from
distributions with long tails. The trimmed mean is a robust estimate.
This means that it is not sensitive to violation of distributional
assumptions of the data. It is called an adaptive estimate when the
trimming proportion is determined from the data rather than being
fixed a “priori-.
The main objective of this study is to find out the robustness
properties of the adaptive trimmed means in terms of efficiency, high
breakdown point and influence function. Specifically, it seeks to find
out the magnitude of the trimming proportion of the adaptive
trimmed mean which will yield efficient and robust estimates of the
parameter for data which follow a modified Weibull distribution with
parameter λ = 1/2 , where the trimming proportion is determined by a
ratio of two trimmed means defined as the tail length. Secondly, the
asymptotic properties of the tail length and the trimmed means are
also investigated. Finally, a comparison is made on the efficiency of
the adaptive trimmed means in terms of the standard deviation for the
trimming proportions and when these were fixed a “priori".
The asymptotic tail lengths defined as the ratio of two trimmed
means and the asymptotic variances were computed by using the
formulas derived. While the values of the standard deviations for the
derived tail lengths for data of size 40 simulated from a Weibull
distribution were computed for 100 iterations using a computer
program written in Pascal language.
The findings of the study revealed that the tail lengths of the
Weibull distribution increase in magnitudes as the trimming
proportions increase, the measure of the tail length and the adaptive
trimmed mean are asymptotically independent as the number of
observations n becomes very large or approaching infinity, the tail
length is asymptotically distributed as the ratio of two independent
normal random variables, and the asymptotic variances decrease as
the trimming proportions increase. The simulation study revealed
empirically that the standard error of the adaptive trimmed mean
using the ratio of tail lengths is relatively smaller for different values
of trimming proportions than its counterpart when the trimming
proportions were fixed a 'priori'.