Abstract: The most demanded organic foods worldwide are those that are consumed fresh, such as fruits and vegetables. However, there is a knowledge gap about some aspects of organic food microbiological quality and safety. Organic fruits and vegetables are more exposed to pathogenic microorganisms due to surface contact with natural fertilizers such as animal manure, wastes and vermicompost used during farming. Therefore, the objective of this work was to study the contamination of organic fresh green leafy vegetables by two emergent pathogens, Arcobacter spp. and Helicobacter pylori. For this purpose, a total of 24 vegetable samples, 13 lettuce and 11 spinach were acquired from 10 different ecological supermarkets and greengroceries and analyzed by culture and PCR. Arcobacter spp. was detected in five samples (20%) by PCR, four spinach and one lettuce. One spinach sample was found to be also positive by culture. For H. pylori, the H. pylori VacA gene-specific band was detected in 12 vegetable samples (50%), 10 lettuces and two spinach. Isolation in the selective medium did not yield any positive result, possibly because of low contamination levels together with the presence of the organism in its viable but non-culturable form. Results showed significant levels of H. pylori and Arcobacter contamination in organic vegetables that are generally consumed raw, which seems to confirm that these foods can act as transmission vehicles to humans.
Abstract: The purpose of this research is to study of consumer
perception and understanding consumer buying behavior that related
between satisfied and factors affecting the purchasing. Methodology
can be classified between qualitative and quantitative approaches for
the qualitative research were interviews from middlemen who bought
organic vegetables, and middlemen related to production and
marketing system. A questionnaire was utilized as a tool to collect
data. Statistics utilized in this research included frequency,
percentage, mean, standard deviation, and multiple regression
analysis. The result show the reason to decision buying motives is
Fresh products of organic vegetables is the most significant factor on
individuals’ income, with a b of –.143, t = –2.470, the price of
organic vegetables is the most significant factor on individuals’
income, with a b of .176, t = 2.561, p value = .011. The results show
that most people with higher income think about the organic products
are expensive and have negative attitudes towards organic vegetable
as individuals with low and medium income level. Therefore,
household income had a significant influence on the purchasing
decision.