Abstract: Essential hypertension (HTN) usually clusters with other cardiovascular risk factors such as age, overweight, diabetes, insulin resistance and dyslipidemia. The target organ damage (TOD) such as left ventricular hypertrophy, microalbuminuria (MA), acute coronary syndrome (ACS), stroke and cognitive dysfunction takes place early in course of hypertension. Though the prevalence of hypertension is high in India, the relationship between microalbuminuria and target organ damage in hypertension is not well studied. This study aim at detecting MA in essential hypertension and its relation to severity of HTN, duration of HTN, body mass index (BMI), age and TOD such as HTN retinopathy and acute coronary syndrome The present study was done in 100 patients of essential hypertension non diabetics admitted to B.L.D.E.University-s Sri B.M.Patil Medical College, Bijapur, from October 2008 to April 2011. The patients underwent detailed history and clinical examination. Early morning 5 ml of urine sample was collected & MA was estimated by immunoturbidometry method. The relationship of MA with the duration & severity of HTN, BMI, age, sex and TOD's like hypertensive retinopathy, ACS was assessed by univariate analysis. The prevalence of MA in this study was found to be 63 %. In that 42% were male & 21% were female. In this study a significant association between MA and the duration of hypertension (p = 0.036) & (OR =0.438). Longer the duration of hypertension, more possibility of microalbumin in urine. Also there was a significant association between severity of hypertension and MA (p=0.045) and (OR=0.093). MA was positive in 50 (79.4%) patients out of 63, whose blood pressure was >160/100 mm Hg. In this study a significant association between MA and the grades of hypertensive retinopathy (p =0.011) and acute coronary syndrome (p = 0.041) (OR =2.805). Gender and BMI did not pose high risk for MA in this study.The prevalence of MA in essential hypertension is high in this part of the community and MA will increase the risk of developing target organ damage.Early screening of patients with essential hypertension for MA and aggressive management of positive cases might reduce the burden of chronic kidney diseases and cardiovascular diseases in the community.
Abstract: A simple method for the simultaneous determination
of hippuric acid and benzoic acid in urine using reversed-phase high
performance liquid chromatography was described. Chromatography
was performed on a Nova-Pak C18 (3.9 x 150 mm) column with a
mobile phase of mixed solution methanol: water: acetic acid
(20:80:0.2) and UV detection at 254 nm. The calibration curve was
linear within concentration range at 0.125 to 6.0 mg/ml of hippuric
acid and benzoic acid. The recovery, accuracy and coefficient
variance of hippuric acid were 104.54%, 0.2% and 0.2% respectively
and for benzoic acid were 98.48%, 1.25% and 0.60% respectively.
The detection limit of this method was 0.01ng/l for hippuric acid and
0.06ng/l for benzoic acid. This method has been applied to the
analysis of urine samples from the suspected of toluene abuser or
glue sniffer among secondary school students at Johor Bahru.