Abstract: The process parameters, temperature, pH and
substrate concentration, were optimized for the production of
gentamicin using Micromonouspora echinospora. Experiments were
carried out according to central composite design in response surface
method. The optimum conditions for the maximum production of
gentamicin were found to be: temperature – 31.7oC, pH – 6.8 and
substrate concentration – 3%. At these optimized conditions the
production of gentamicin was found to be – 1040 mg/L. The R2 value
of 0.9465 indicates a good fitness of the model.