Abstract: A simple method for the simultaneous determination
of hippuric acid and benzoic acid in urine using reversed-phase high
performance liquid chromatography was described. Chromatography
was performed on a Nova-Pak C18 (3.9 x 150 mm) column with a
mobile phase of mixed solution methanol: water: acetic acid
(20:80:0.2) and UV detection at 254 nm. The calibration curve was
linear within concentration range at 0.125 to 6.0 mg/ml of hippuric
acid and benzoic acid. The recovery, accuracy and coefficient
variance of hippuric acid were 104.54%, 0.2% and 0.2% respectively
and for benzoic acid were 98.48%, 1.25% and 0.60% respectively.
The detection limit of this method was 0.01ng/l for hippuric acid and
0.06ng/l for benzoic acid. This method has been applied to the
analysis of urine samples from the suspected of toluene abuser or
glue sniffer among secondary school students at Johor Bahru.