Abstract: The objective of this study was to investigate hydrogen production from alcohol wastewater by anaerobic sequencing batch reactor (ASBR) under thermophillic operation. The ASBR unit used in this study had a liquid holding volume of 4 L and was operated at 6 cycles per day. The seed sludge taken from an upflow anaerobic sludge blanket unit treating the same wastewater was boiled at 95 °C for 15 min before being fed to the ASBR unit. The ASBR system was operated at different COD loading rates at a thermophillic temperature (55 °C), and controlled pH of 5.5. When the system was operated under optimum conditions (providing maximum hydrogen production performance) at a feed COD of 60 000 mg/l, and a COD loading rate of 68 kg/m3 d, the produced gas contained 43 % H2 content in the produced gas. Moreover, the hydrogen yield and the specific hydrogen production rate (SHPR) were 130 ml H2/g COD removed and 2100 ml H2/l d, respectively.
Abstract: In this work, biohydrogen production via dark
fermentation from alcohol wastewater using upflow anaerobic sludge
blanket reactors (UASB) with a working volume of 4 L was
investigated to find the optimum conditions for a maximum hydrogen
yield. The system was operated at different COD loading rates (23,
31, 46 and 62 kg/m3d) at mesophilic temperature (37 ºC) and pH 5.5.
The seed sludge was pretreated before being fed to the UASB system
by boiling at 95 ºC for 15 min. When the system was operated under
the optimum COD loading rate of 46 kg/m3d, it provided the
hydrogen content of 27%, hydrogen yield of 125.1 ml H2/g COD
removed and 95.1 ml H2/g COD applied, hydrogen production rate of
18 l/d, specific hydrogen production rate of 1080 ml H2/g MLVSS d
and 1430 ml H2/ L d, and COD removal of 24%.