Abstract: Presently a significant portion of the Earth's
population does not have access to healthy food. Either because they
cannot afford it or because they do not know which one are they. The
aim of the VII th Framework Chance project (Nr. 266331) supported
by the European Union has been to develop relatively cheap food
with favourable nutritional value and it should have acceptable
quality for consumers. As one task of the project we manufactured
bread products as a basic food. We examined the enrichment of bread
products with four kinds of bran, with a special milling product of
grain industry (aleurone-rich flour) and with a soy-based sprouted
additive. The applied concentration of the six mentioned additives
has been optimized and the physical properties of the bread products
were monitored. The weight/density of the enriched breads increased
a bit, however the volume and height decreased slightly compared to
the corresponding data of the control bread. The optimized
composition of the final product is favourably affected by these
additives having highly preferred composition from nutritional point
of view.
Abstract: More than 3000 plants of notable phyto-therapeutic
value grow in South Africa; these include Cissampelos capensis,
commonly known in Afrikaans as dawidjie or dawidjiewortel. C.
capensis is the most significant and popular medicinal plant used by
the Khoisan as well as other rural groups in the Western region of
South Africa. Its rhizomes are traditionally used to treat male fertility
problems. Yet, no studies have investigated the effects of this plant or
its extracts on human spermatozoa. Therefore, this study aimed at
investigating the effects of C. capensis rhizome extract (CRE)
fractions on ejaculated human spermatozoa in vitro. Spermatozoa
from a total of 77 semen samples were washed with human tubular
fluid medium supplemented with bovine serum albumin (HTF-BSA)
and incubated for 2 hours with 20 μg/ml progesterone (P4) followed
by incubation with different concentrations (0, 0.05, 0.5, 5, 50, 200
μg/ml) of fractionated CRE (F1=0% MeOH, F2=30% MeOH,
F3=60% MeOH and F4=100% MeOH) for 1.5 hours at 37°C. A
sample without addition of CRE fractions served as control. Samples
were analyzed for sperm motility, reactive oxygen species (ROS),
DNA-fragmentation, acrosome reaction and capacitation. Results
showed that F1 resulted in significantly higher values for ROS,
capacitation and hyper-activation compared to F2, F3, and F4 with
P4-stimulated samples generally having higher values. No significant
effect was found for the other parameters. In conclusion, alkaloids
present in F1 of CRE appear to have triggered sperm intrinsic ROS
production leading to sperm capacitation and acrosome reaction
induced by P4.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: Quantitative analyses of whisker movements provide a
means to study functional recovery and regeneration of mouse facial
nerve after an injury. However, accurate tracking of the mouse whisker
movement is challenging. Most methods for whisker tracking require
manual intervention, e.g. fixing the head of the mouse during a study.
Here we describe a semi-automated image processing method, which
is applied to high-speed video recordings of free-moving mice to track
the whisker movements. We first track the head movement of a mouse
by delineating the lower head contour frame-by-frame that allows for
detection of the location and orientation of the head. Then, a region of
interest is identified for each frame; the subsequent application of a
mask and the Hough transform detects the selected whiskers on each
side of the head. Our approach is used to examine the functional
recovery of damaged facial nerves in mice over a course of 21 days.
Abstract: Durian is the flagship fruit of Mindanao and there is
an abundance of several cultivars with many confusing identities/
names.
The project was conducted to develop procedure for reliable and
rapid detection and sorting of durian planting materials. Moreover, it
is also aimed to establish specific genetic or DNA markers for routine
testing and authentication of durian cultivars in question.
The project developed molecular procedures for routine testing.
SSR primers were also screened and identified for their utility in
discriminating durian cultivars collected.
Results of the study showed the following accomplishments:
1. Twenty (29) SSR primers were selected and identified based on
their ability to discriminate durian cultivars,
2. Optimized and established standard procedure for identification
and authentication of Durian cultivars
3. Genetic profile of durian is now available at Biotech Unit
Our results demonstrate the relevance of using molecular
techniques in evaluating and identifying durian clones. The most
polymorphic primers tested in this study could be useful tools for
detecting variation even at the early stage of the plant especially for
commercial purposes. The process developed combines the efficiency
of the microsatellites development process with the optimization of
non-radioactive detection process resulting in a user-friendly protocol
that can be performed in two (2) weeks and easily incorporated into
laboratories about to start microsatellite development projects. This
can be of great importance to extend microsatellite analyses to other
crop species where minimal genetic information is currently
available. With this, the University can now be a service laboratory
for routine testing and authentication of durian clones.
Abstract: The grain quality of chickpea in Iran is low and
instable, which may be attributed to the evolution of cultivars with a
narrow genetic base making them vulnerable to biotic stresses. Four
chickpea varieties from diverse geographic origins were chosen and
arranged in a randomized complete block design. Mesorhizobium sp.
cicer strain SW7 was added to all the chickpea seeds. Chickpea seeds
were planted on October 9, 2013. Each genotype was sown 5 m in
length, with 35 cm inter-row spacing, in 3 rows. Weeds were
removed manually in all plots. Results showed that Analysis of
variance on the studied traits showed significant differences among
genotypes for N, P, K and Fe contents of chickpea, but there is not a
significant difference among Ca, Zn and Mg continents of chickpea.
The experimental coefficient of variation (CV) varied from 7.3 to
15.8. In general, the CV value lower than 20% is considered to be
good, indicating the accuracy of conducted experiments. The highest
grain N was observed in Hashem and Jam cultivars. The highest grain
P was observed in Jam cultivar. Phosphorus content (mg/100g)
ranged from 142.3 to 302.3 with a mean value of 221.3. The negative
correlation (-0.126) was observed between the N and P of chickpea
cultivars. The highest K and Fe contents were observed in Jam
cultivar.
Abstract: Fruit juices play important roles in human health as
being a key part of nutrition. Juice and nectar are two categories of
drinks with so many variations for consumers, regardless of age,
lifestyle and taste preferences, which they can find their favorites.
Juices contain 100% pulp when pulp content of ‘nectar’ changes
between 25%-50%. In this study, potassium (K), magnesium (Mg),
and phosphorus (P) contents in orange juice and nectar is determined
for conscious consumption. For this purpose inductively coupled
plasma optical emission spectrometry (ICP-OES) is used to find out
potassium (K), magnesium (Mg), and phosphorus (P) contents in
orange juices and nectar. Furthermore, the daily intake of elements
from orange juice and nectar that affects human health is also
investigated. From the results of experiments K, Mg and P contents
are found in orange juice as 1351; 73,25; 89,27 ppm and in orange
nectar as 986; 33,76; 51,30 respectively.
Abstract: In this work, two fermentations at different
temperatures (25 and 30ºC), with cell recycling, were accomplished
to produce ethanol, using a mix of commercial substrates, xylose
(70%) and glucose (30%), as organic source for Scheffersomyces
stipitis. Five consecutive fermentations of 80 g L-1 (1º, 2º and 3º
recycles), 96 g L-1 (4º recycle) and 120 g L-1 (5º recycle)reduced
sugars led to a final maximum ethanol concentration of 17.2 and 34.5
g L-1, at 25 and 30ºC, respectively. Glucose was the preferred
substrate; moreover xylose startup degradation was initiated after a
remaining glucose presence in the medium. Results showed that yeast
acid treatment, performed before each cycle, provided improvements
on cell viability, accompanied by ethanol productivity of 2.16 g L-1 h-
1 at 30ºC. A maximum 36% of xylose was retained in the
fermentation medium and after five-cycle fermentation an ethanol
yield of 0.43 g ethanol/g sugars was observed. S. stipitis fermentation
capacity and tolerance showed better results at 30ºC with 83.4% of
theoretical yield referenced on initial biomass.
Abstract: This work assesses the cortical and the sub-cortical
neural activity recorded from rodents using entropy and mutual
information based approaches to study how hypothermia affects neural
activity. By applying the multi-scale entropy and Shannon entropy, we
quantify the degree of the regularity embedded in the cortical and
sub-cortical neurons and characterize the dependency of entropy of
these regions on temperature. We study also the degree of the mutual
information on thalamocortical pathway depending on temperature.
The latter is most likely an indicator of coupling between these highly
connected structures in response to temperature manipulation leading
to arousal after global cerebral ischemia.
Abstract: Mangosteen (Garcinia mangostana) pericarp is
considered as agricultural waste and not fully utilized in food
products. It is widely reported that mangosteen pericarp contains high
antioxidant properties. The objective of this study is to develop novel
yellow alkaline noodle (YAN) substituted with different levels of
mangosteen pericarp powder (MPP). YAN formulation was
substituted with different levels of MPP (0%, 5%, 10% and 15%).
The effect on nutritional and antioxidant properties was evaluated.
Higher substitution levels of MPP resulted in significant increase
(p
Abstract: A field study was conducted to evaluate the efficacy
of lavender for phytoremediation of contaminated soils. The
experiment was performed on an agricultural fields contaminated by
the Non-Ferrous-Metal Works near Plovdiv, Bulgaria. The
concentrations of Pb, Zn and Cd in lavender (roots, stems, leaves and
inflorescences) and in the essential oils of lavender were determined.
Lavender is a plant which is tolerant to heavy metals and can be
grown on contaminated soils, and which can be referred to the
hyperaccumulators of lead and the accumulators of cadmium and
zinc, and can be successfully used in the phytoremediation of heavy
metal contaminated soils. Favorable is also the fact that heavy metals
do not influence the development of the lavender, as well as on the
quality and quantity of the essential oil. The possibility of further
industrial processing will make lavender economically interesting
crops for farmers of phytoextraction technology.
Abstract: A total of 115 yeast strains isolated from local cassava
processing wastes were measured for crude protein content. Among
these strains, the strain MSY-2 possessed the highest protein
concentration (>3.5 mg protein/mL). By using molecular
identification tools, it was identified to be a strain of Pichia
kudriavzevii based on similarity of D1/D2 domain of 26S rDNA
region. In this study, to optimize the protein production by MSY-2
strain, Response Surface Methodology (RSM) was applied. The
tested parameters were the carbon content, nitrogen content, and
incubation time. Here, the value of regression coefficient (R2) =
0.7194 could be explained by the model which is high to support the
significance of the model. Under the optimal condition, the protein
content was produced up to 3.77 g per L of the culture and MSY-2
strain contains 66.8 g protein per 100 g of cell dry weight. These
results revealed the plausibility of applying the novel strain of yeast
in single-cell protein production.
Abstract: Hydrothermal liquefaction (HTL) is a technique for obtaining clean biofuel from biomass in the presence of heat and pressure in an aqueous medium which leads to a decomposition of this biomass to the formation of various products. A role of operating conditions is essential for the bio-oil and other products’ yield and also quality of the products. The effects of these parameters were investigated in regards to the composition and yield of the products. Chlorellaceae microalgae were tested under different HTL conditions to clarify suitable conditions for extracting bio-oil together with value-added co-products. Firstly, different microalgae loading rates (5-30%) were tested and found that this parameter has not much significant to product yield. Therefore, 10% microalgae loading rate was selected as a proper economical solution for conditioned schedule at 250oC and 30 min-reaction time. Next, a range of temperature (210-290oC) was applied to verify the effects of each parameter by keeping the reaction time constant at 30 min. The results showed no linkage with the increase of the reaction temperature and some reactions occurred that lead to different product yields. Moreover, some nutrients found in the aqueous product are possible to be utilized for nutrient recovery.
Abstract: Lignocellolusic material is a substance that is resistant to be degraded by microorganisms or hydrolysis enzymes. To be used as materials for biofuel production, it needs pretreatment process to improve efficiency of hydrolysis. In this work, chemical pretreatments on rice straw using three diluted organic acids, including acetic acid, citric acid, oxalic acid, were optimized. Using Response Surface Methodology (RSM), the effect of three pretreatment parameters, acid concentration, treatment time, and reaction temperature, on pretreatment efficiency were statistically evaluated. The results indicated that dilute oxalic acid pretreatment led to the highest enhancement of enzymatic saccharification by commercial cellulase and yielded sugar up to 10.67 mg/ml when using 5.04% oxalic acid at 137.11 oC for 30.01 min. Compared to other acid pretreatment by acetic acid, citric acid, and hydrochloric acid, the maximum sugar yields are 7.07, 6.30, and 8.53 mg/ml, respectively. Here, it was demonstrated that organic acids can be used for pretreatment of lignocellulosic materials to enhance of hydrolysis process, which could be integrated to other applications for various biorefinery processes.
Abstract: Rice straw is lignocellulosic biomass which can be utilized as substrate for the biogas production. However, due to the property and composition of rice straw, it is difficult to be degraded by hydrolysis enzymes. One of the pretreatment methods that modify such properties of lignocellulosic biomass is the application of lignocellulose-degrading microbial consortia. The aim of this study is to investigate the effect of microbial consortia to enhance biogas production. To select the high efficient consortium, cellulase enzymes were extracted and their activities were analyzed. The results suggested that microbial consortium culture obtained from cattle manure is the best candidate compared to decomposed wood and horse manure. A microbial consortium isolated from cattle manure was then mixed with anaerobic sludge and used as inoculum for biogas production. The optimal conditions for biogas production were investigated using response surface methodology (RSM). The tested parameters were the ratio of amount of microbial consortium isolated and amount of anaerobic sludge (MI:AS), substrate to inoculum ratio (S:I) and temperature. Here, the value of the regression coefficient R2 = 0.7661 could be explained by the model which is high to advocate the significance of the model. The highest cumulative biogas yield was 104.6 ml/g-rice straw at optimum ratio of MI:AS, ratio of S:I, and temperature of 2.5:1, 15:1 and 44°C respectively.
Abstract: Honeys are produced by Apis mellifera and stingless
bees (Meliponini) in Ecuador. We studied honey produced in
beeswax combs by Apis mellifera, and honey produced in pots by
Geotrigona and Scaptotrigona bees. Chloroform extracts of honey
were obtained for fast NMR spectra. The 1D spectra were acquired at
298 K, with a 600 MHz NMR Bruker instrument, using a modified
double pulsed field gradient spin echoes (DPFGSE) sequence.
Signals of 1H NMR spectra were integrated and used as inputs for
PCA, PLS-DA analysis, and labelled sets of classes were successfully
identified, enhancing the separation between the three groups of
honey according to the entomological origin: A. mellifera,
Geotrigona and Scaptotrigona. This procedure is therefore
recommended for authenticity test of honey in Ecuador.
Abstract: In this research the effect of moisture at three levels
(47, 57, and 67 w.b.%) on the physical properties of the Pofaki pea
variety including, dimensions, geometric mean diameter, volume,
sphericity index and the surface area was determined. The influence
of different moisture levels (47, 57 and 67 w.b.%), in two loading
orientation (longitudinal and transverse) and three loading speed (4,6
and 8 mm min-1) on the mechanical properties of pea such as
maximum deformation, rupture force, rupture energy, toughness and
the power to break the pea was investigated. It was observed in the
physical properties that moisture changes were affective at 1% on,
dimensions, geometric mean diameter, volume, sphericity index and
the surface area. It was observed in the mechanical properties that
moisture changes were effective at 1% on, maximum deformation,
rupture force, rupture energy, toughness and the power to break.
Loading speed was effective on maximum deformation, rupture
force, rupture energy at 1% and it was effective on toughness at 5%.
Loading orientation was effective on maximum deformation, rupture
force, rupture energy, toughness at 1% and it was effective on power
at 5%. The mutual effect of speed and orientation were effective on
rupture energy at 1% and were effective on toughness at 5%
probability. The mutual effect of moisture and speed were effective
on rupture force and rupture energy at 1% and were effective on
toughness 5% probability. The mutual effect of orientation and
moisture on rupture energy and toughness were effective at 1%.
Abstract: The quality and condition of perishable products
delivered to the market and their subsequent selling prices are
directly affected by the care taken during harvesting and handling.
Mechanical injury, in fact, occurs at all stages, from pre-harvest
operations through post-harvest handling, packing and transport to
the market. The main implications of this damage are the reduction of
the product’s quality and economical losses related to the shelf life
diminution. For most perishable products, the shelf life is relatively
short and it is typically dictated by microbial growth related to the
application of dynamic and static loads during transportation. This
paper presents the correlation between vibration levels and
microbiological growth on strawberries and woodland strawberries
and detects the presence of volatile organic compounds (VOC) in
order to develop an intelligent logistic unit capable of monitoring
VOCs using a specific sensor system. Fresh fruits were exposed to
vibrations by means of a vibrating table in a temperature-controlled
environment. Microbiological analyses were conducted on samples,
taken at different positions along the column of the crates. The values
obtained were compared with control samples not exposed to
vibrations and the results show that different positions along the
column influence the development of bacteria, yeasts and filamentous
fungi.
Abstract: Microalgae Meyerella planktonica is a potential
biofuel source because it can grow in bulk in either autotrophic or
heterotrophic condition. However, the quantitative growth of this
algal type is still low as it tends to precipitates on the bottom.
Besides, the lipid concentration is still low when grown in
autotrophic condition. In contrast, heterotrophic condition can
enhance the lipid concentration. The combination of autotrophic
condition and agitation treatment was conducted to increase the
density of the culture. On the other hand, a heterotrophic condition
was set up to raise the lipid production. A two-stage experiment
was applied to increase the density at the first step and to increase
the lipid concentration in the next step. The autotrophic condition
resulted higher density but lower lipid concentration compared to
heterotrophic one. The agitation treatment produced higher density
in both autotrophic and heterotrophic conditions. The two-stage
experiment managed to enhance the density during the autotrophic
stage and the lipid concentration during the heterotrophic stage.
The highest yield was performed by using 0.4% v/v glycerol as a
carbon source (2.9±0.016 x 10^6 cells w/w) attained 7 days after the
heterotrophic stage began. The lipid concentration was stable
starting from day 7.
Abstract: In 2010-2011, the research on zooplankton was
conducted in the southern part of the Baltic Sea to determine seasonal
variability in changes occurring throughout the zooplankton in 2010
and 2011, both in the region of Gdańsk Deep, and in the western part
of Gdańsk Bay. The research in the sea showed that the taxonomic
composition of holoplankton in the southern part of the Baltic Sea
was similar to that recorded in this region for many years. The
maximum values of abundance and biomass of zooplankton both in
the Deep and the Bay of Gdańsk were observed in the summer
season. Copepoda dominated in the composition of zooplankton for
almost the entire study period, while rotifers occurred in larger
numbers only in the summer 2010 in the Gdańsk Deep as well as in
May and July 2010 in the western part of Gdańsk Bay, and
meroplankton – in April 2011.