Abstract: This study examined the toxicological effects and
safety of polypeptide k isolated from the seeds of Momordica
charantia in laboratory rats. 30 male Sprague Dawley rats (12 weeks
old, bodyweight 180-200 g) were randomly divided into 3 groups
(1000 mg/kg, 500 mg and 0 mg/kg). Rats were acclimatized to
laboratory conditions for 7 days and at day 8 rats were dosed orally
with polypeptide k (in 2% DMSO/normal saline) and the controls
received the dosed vehicle only. Rats were then observed for 72
hours before sacrificed. Rats were anaesthetized by pentobarbital
(50 mg/kg ip) and 2-3.0 mL of blood was taken by cardiac puncture
and rats were scarified by anaesthetic overdose. Immediately, organs
(heart, lungs, liver, kidneys) were weigh and taken for histology.
Organ sections were then evaluated by a histopathologist. Serum
samples were assayed for liver functions (ALT and γ-GT) and kidney
functions (BUN and creatinine). All rats showed normal behavior
after the dosing and no statistical changes were observed in all blood
parameters and organ weight. Histological examinations revealed
normal organ structures. In conclusion, dosing of rats up to 1000
mg/kg did not have any effects on the rat behavior, liver or kidney
functions nor histology of the selected organs.
Abstract: The present study was carried out to evaluate the
nutritional value of sorghum flour during processing of injera
(unleavened thick bread). The proximate composition of sorghum
flour before and after fermentation and that of injera was determined.
Compared to the raw flour and fermented one, injera had low protein
(11.55%), ash (1.57%) and fat (2.40%) contents but high in fiber
content. Moreover, injera was found to have significantly (P ≤ 0.05)
higher energy (389.08 Kcal/100g) compared to raw and fermented
sorghum flour. Injera contained lower levels of anti-nutritional
factors (polyphenols, phytate and tannins) compared to raw and
fermented sorghum. Also it was found to be rich in Ca
(4.75mg/100g), Fe (3.95 mg/100g), and Cu (0.7 mg/100g) compared
to that of raw and fermented flour. Moreover, both the extractable
minerals and protein digestibility were high for injera due to low
amount of anti-nutrients. Injera was found to contain an appreciable
amount of amino acids except arginine and tyrosine.
Abstract: Segmentation, filtering out of measurement errors and
identification of breakpoints are integral parts of any analysis of
microarray data for the detection of copy number variation (CNV).
Existing algorithms designed for these tasks have had some successes
in the past, but they tend to be O(N2) in either computation time or
memory requirement, or both, and the rapid advance of microarray
resolution has practically rendered such algorithms useless. Here we
propose an algorithm, SAD, that is much faster and much less thirsty
for memory – O(N) in both computation time and memory requirement
-- and offers higher accuracy. The two key ingredients of SAD are the
fundamental assumption in statistics that measurement errors are
normally distributed and the mathematical relation that the product of
two Gaussians is another Gaussian (function). We have produced a
computer program for analyzing CNV based on SAD. In addition to
being fast and small it offers two important features: quantitative
statistics for predictions and, with only two user-decided parameters,
ease of use. Its speed shows little dependence on genomic profile.
Running on an average modern computer, it completes CNV analyses
for a 262 thousand-probe array in ~1 second and a 1.8 million-probe
array in 9 seconds
Abstract: The whole-cell protein-profiling technique was
evaluated for studying differences in banding pattern of three
different species of Cyanobacteria i.e. Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica under the influence
of four different pesticides-2,4-D (Ethyl Ester of 2,4-Dichloro
Phenoxy Acetic Acid), Pencycuron (N-[(4-chlorophenyl)methyl]-Ncyclopentyl-
N'–phenylurea), Endosulfan (6,7,8,9,10,10hexachloro-
1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-
oxide) and Tebuconazole (1-(4-Chlorophenyl)-4,4-dimethyl-3-(1,2,4-
triazol-1-ylmethyl)pentan-3-ol). Whole-cell extracts were obtained by
sonication treatment (Sonifier cell disruptor -Branson Digital Sonifier
S-450D, USA) and were analyzed by sodium dodecyl sulfate
polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE
analyses of the total protein profile of Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica showed a linear
decrease in the protein content with increasing pesticide stress when
administered to different concentrations of 2, 4-D, Pencycuron,
Endosulfan and Tebuconazole. The results indicate that different
stressors exert specific effects on cyanobacterial protein synthesis.
Abstract: Relevant agricultural information disseminator
(extension agent) ratio of 1:3500 farm families which become a
menace to agricultural production capacity in developing countries
necessitate this study. Out of 4 zones in the state, 24 extension agents
in each zone, 4 extension agents using cell phones and 120 farmers
using cell phone and 120 other farmers not using cell phone were
purposively selected to give 240 farmers that participated in the
research. Data were collected using interview guide and analysized
using frequency, percentage and t-test.. Frequency of contact with
agricultural information centers revealed that cell phone user farmers
had greater means score of X 41.43 contact as against the low mean
X19.32 contact recorded by farmers receiving agricultural
information from extension agents not using cell phone and their
production was statistically significant at P < 0.05. Usage of cell
phone increase extension agent contact and increase farmers-
production capacity.
Abstract: A cross sectional study design and standard
microbiological procedures were used to determine the prevalence
and antimicrobial susceptibility patterns of Escherichia coli,
Salmonella enterica serovar typhimurium and Vibrio cholerae O1
isolated from water and two fish species Rastrineobola argentea and
Oreochromis niloticus collected from fish landing beaches and
markets in the Lake Victoria Basin of western Kenya. Out of 162
samples analyzed, 133 (82.1%) were contaminated, with S.
typhimurium as the most prevalent (49.6%), followed by E. coli
(46.6%), and lastly V. cholerae (2.8%). All the bacteria isolates were
sensitive to ciprofloxacin. E. coli isolates were resistant to ampicillin,
tetracycline, cotrimoxazole, chloramphenical and gentamicin while
S. typhimurium isolates exhibited resistance to ampicillin,
tetracycline, and cotrimoxazole. The V. cholerae O1 isolates were
resistant to tetracycline and ampicillin. The high prevalence of drug
resistant enteric bacteria in water and fish from the study region
needs public health intervention from the local government.
Abstract: The aim of this in vitro study was to evaluate the possible interference of a Nectandra membranacea extract (i) on the labeling of blood cells (BC), (ii) on the labeling process of BC and plasma (P) proteins with technetium-99m (Tc-99m) and (iii) on the morphology of red blood cells (RBC). Blood samples were incubated with a Nectandra membranacea crude extract, stannous chloride, Tc- 99m (sodium pertechnetate) was added, and soluble (SF) and insoluble (IF) fractions were isolated. Morphometry studies were performed with blood samples incubated with Nectandra membranacea extract. The results show that the Nectandra membranacea extract does not promote significant alteration of the labeling of BC, IF-P and IF-BC. The Nectandra membranacea extract was able to alter the erythrocyte membrane morphology, but these morphological changes were not capable to interfere on the labeling of blood constituents with Tc-99m.
Abstract: The present work was conducted to find out the effect
of biofertilizer formulated with four species of bacteria (two species
of Azotobacter and two species of Lysobacter) and zinc sulphate.
Field experiments with mustard plant were conducted to study the
effectiveness of soil application of zinc sulphate and biofertilizer at
0, 10, 20, 30, 40, 50 days after sowing. Plant height and condition of
plant was found to be increased significantly using a mixture of
biofertilizer and zinc sulphate than other treatments after 40 days
sowing. Three treatments were also used in this field experiment such
as bacteria only, zinc sulphate only and mixture of biofertilizer and
zinc sulphate. The treatment using a mixture of zinc sulphate and
biofertilizer had the best yield (4688.008 kg/ha) within 50 days of
sowing and performed better than other treatments. Field experiment
using zinc sulphate only was second best yield (3380.75Kg/ha) and
biofertilizer only treatment gave (2639.04kg/ha).
Abstract: A New features are extracted and compared to
improve the prediction of protein-protein interactions. The basic idea
is to select and use the best set of features from the Tensor matrices
that are produced by the frequency vectors of the protein sequences.
Three set of features are compared, the first set is based on the
indices that are the most common in the interacting proteins, the
second set is based on the indices that tend to be common in the
interacting and non-interacting proteins, and the third set is
constructed by using random indices. Moreover, three encoding
strategies are compared; that are based on the amino asides polarity,
structure, and chemical properties. The experimental results indicate
that the highest accuracy can be obtained by using random indices
with chemical properties encoding strategy and support vector
machine.
Abstract: MicroRNAs (miRNAs) are small, non-coding and
regulatory RNAs about 20 to 24 nucleotides long. Their conserved
nature among the various organisms makes them a good source of
new miRNAs discovery by comparative genomics approach. The
study resulted in 21 miRNAs of 20 pre-miRNAs belonging to 16
families (miR156, 157, 158, 164, 165, 168, 169, 172, 319, 390, 393,
394, 395, 400, 472 and 861) in evergreen spruce tree (Picea). The
miRNA families; miR 157, 158, 164, 165, 168, 169, 319, 390, 393,
394, 400, 472 and 861 are reported for the first time in the Picea. All
20 miRNA precursors form stable minimum free energy stem-loop
structure as their orthologues form in Arabidopsis and the mature
miRNA reside in the stem portion of the stem loop structure. Sixteen
(16) miRNAs are from Picea glauca and five (5) belong to Picea
sitchensis. Their targets consist of transcription factors, growth
related, stressed related and hypothetical proteins.
Abstract: The present work represents an investigation of the
hydrolysis of hull-less pumpkin (Cucurbita Pepo L.) oil cake protein
isolate (PuOC PI) by pepsin. To examine the effectiveness and
suitability of pepsin towards PuOC PI the kinetic parameters for
pepsin on PuOC PI were determined and then, the hydrolysis process
was studied using Response Surface Methodology (RSM). The
hydrolysis was carried out at temperature of 30°C and pH 3.00. Time
and initial enzyme/substrate ratio (E/S) at three levels were selected
as the independent parameters. The degree of hydrolysis, DH, was
mesuared after 20, 30 and 40 minutes, at initial E/S of 0.7, 1 and 1.3
mA/mg proteins. Since the proposed second-order polynomial model
showed good fit with the experimental data (R2 = 0.9822), the
obtained mathematical model could be used for monitoring the
hydrolysis of PuOC PI by pepsin, under studied experimental
conditions, varying the time and initial E/S. To achieve the highest
value of DH (39.13 %), the obtained optimum conditions for time
and initial E/S were 30 min and 1.024 mA/mg proteins.
Abstract: Salinity level may affect early development of
biofuel feedstock crops. The biofuel feedstock crops canola
(Brassica napus L.), sorghum [Sorghum bicolor (L.) Moench], and
sunflower (Helianthus annuus L.); and the potential feedstock crop
sweet corn (Zea mays L.) were planted in media in pots and treated
with aqueous solutions of 0, 0.1, 0.5 and 1.0 M NaCl once at: 1)
planting; 2) 7-10 days after planting or 3) first true leaf expansion.
An additional treatment (4) comprised of one-half strength of the 0.1,
0.5 and 1.0 M (concentrations 0.05, 0.25, 0.5 M at each application)
was applied at first true leaf expansion and four days later. Survival
of most crops decreased below 90% above 0.5 M; survival of canola
decreased above 0.1 M. Application timing had little effect on crop
survival. For canola root fresh and dry weights improved when
application was at plant emergence; for sorghum top and root fresh
weights improved when the split application was used. When
application was at planting root dry weight was improved over most
other applications. Sunflower top fresh weight was among the
highest when saline solutions were split and top dry weight was
among the highest when application was at plant emergence. Sweet
corn root fresh weight was improved when the split application was
used or application was at planting. Sweet corn root dry weight was
highest when application was at planting or plant emergence. Even at
high salinity rates survival rates greater than what might be expected
occurred. Plants that survived appear to be able to adjust to saline
during the early stages of development.
Abstract: Application of pesticides in the paddy fields has
deleterious effects on non-target organisms including cyanobacteria
which are photosynthesizing and nitrogen fixing micro-organisms
contributing significantly towards soil fertility and crop yield.
Pesticide contamination in the paddy fields has manifested into a
serious global environmental concern. To study the effect of one such
pesticide, three cyanobacterial strains; Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica were selected for
their stress responses to an Organochlorine insecticide - 6, 7, 8, 9, 10,
10-hexachloro-1, 5, 5a, 6, 9, 9a-hexahydro-6, 9-methano-2, 4, 3-
benzodioxathiepine-3-oxide, with reference to their photosynthesic
pigments-chlorophyll-a and carotenoids as well as accessory
pigments-phycobiliproteins (phycocyanin, allophycocyanin and
phycoerythrin), stress induced biochemical metabolites like
carbohydrates, proteins, amino acids, phenols and enzymes-nitrate
reductase, glutamine synthetase and succinate dehydrogenase. All
the three cyanobacterial strains were adversely affected by the
insecticide doses and inhibition was dose dependent. Reduction in
photosynthetic and accessory pigments, metabolites, nitrogen fixing
and respiratory enzymes of the test organisms were accompanied
with an initial increase in their total protein at lower Organochlorine
doses. On the other hand, increased amount of phenols in all the
insecticide treated concentrations was indicative of stressed activities
of the organisms.
Abstract: Plackett-Burman statistical screening of media
constituents and operational conditions for extracellular lipase
production from isolate Trichoderma viride has been carried out in
submerged fermentation. This statistical design is used in the early
stages of experimentation to screen out unimportant factors from a
large number of possible factors. This design involves screening of
up to 'n-1' variables in just 'n' number of experiments. Regression
coefficients and t-values were calculated by subjecting the
experimental data to statistical analysis using Minitab version 15.
The effects of nine process variables were studied in twelve
experimental trials. Maximum lipase activity of 7.83 μmol /ml /min
was obtained in the 6th trail. Pareto chart illustrates the order of
significance of the variables affecting the lipase production. The
present study concludes that the most significant variables affecting
lipase production were found to be palm oil, yeast extract, K2HPO4,
MgSO4 and CaCl2.
Abstract: In order to investigate water deficit stress on 24 of
soybean (Glycine Max. L) cultivars and lines in temperate climate, an
experiment was conducted in Iran Seed and Plant Improvement
Institute. Stress levels were irrigation after evaporation of 50, 100,
150 mm water from pan, class A. Randomized Completely Block
Design was arranged for each stress levels. Some traits such as, node
number, plant height, pod number per area, grain number per pod,
grain number per area, 1000 grains weight, grain yield and harvest
index were measured. Results showed that water deficit stress had
significant effect on node number, plant height, pod number per area,
grain number per pod, grain number per area, 1000 grains weight and
harvest index. Also all of agronomic traits except harvest index
influenced significantly by cultivars and lines. The least and most
grain yield was belonged to Ronak X Williams and M41 x Clark
respectively.