Abstract: In mammalian reproductive tract, the oviduct secretes
huge number of growth factors and cytokines that create an optimal
micro-environment for the initial stages of preimplantation embryos.
Secretion of these growth factors is stage-specific. Among them,
VEGF is a potent mitogen for vascular endothelium and stimulates
vascular permeability. Apart from angiogenesis, VEGF in the oviduct
may be involved in regulating the oocyte maturation and subsequent
developmental process during embryo production in vitro. In
experiment 1, to evaluate the effect of VEGF during IVM of porcine
COC and subsequent developmental ability after PA and SCNT. The
results from these experiments indicated that maturation rates among
the different VEGF concentrations were not significant different. In
experiment 2, total intracellular GSH concentrations of oocytes
matured with VEGF (5-50 ng/ml) were increased significantly
compared to a control and VEGF group (500 ng/ml). In experiment 3,
the blastocyst formation rates and total cell number per blastocyst
after parthenogenesis of oocytes matured with VEGF (5-50 ng/ml)
were increased significantly compared to a control and VEGF group
(500 ng/ml). Similarly, in experiment 4, the blastocyst formation rate
and total cell number per blastocyst after SCNT and IVF of oocytes
matured with VEGF (5 ng/ml) were significantly higher than that of
oocytes matured without VEGF group. In experiment 5, at 10 hour
after the onset of IVF, pronuclear formation rate was evaluated.
Monospermy was significantly higher in VEGF-matured oocytes than
in the control, and polyspermy and sperm penetration per oocyte
were significantly higher in the control group than in the VEGFmatured
oocytes. Supplementation with VEGF during IVM
significantly improved male pronuclear formation as compared with
the control. In experiment 6, type III cortical granule distribution in
oocytes was more common in VEGF-matured oocytes than in the
control. In conclusion, the present study suggested that
supplementation of VEGF during IVM may enhance the
developmental potential of porcine in vitro embryos through increase
of the intracellular GSH level, higher MPN formation and increased
fertilization rate as a consequence of an improved cytoplasmic
maturation.
Abstract: Scolothrips longicornis Priesner is one of the
important predators of tetranychid mites with a wide distribution
throughout Iran. Life table and population growth parameters of S.
longicornis feeding on two-spotted spider mite, Tetranychus
turkestani Ugarov & Nikolski were investigated under laboratory
condition (26±1ºC, 65±5% R.H. and 16L: 8D). To carry of these
experiments, S. longicornis collections reared on cowpea infested
with T. turkestani were prepared. The eggs with less than 24 hours
old were selected and reared. The emerged larvae feeding directly on
cowpea leaf discs which were infested with T. turkestani. Thirty
females of S. longicornis with 24 hours age were selected and
released on infested leaf discs. They replaced daily to a new leaf disc
and the laying eggs have counted. The experiment continued till the
last thrips had died. The result showed that the mean age mortality of
the adult female thrips were between 21-25 days which is nearly
equal life expectancy (ex) at the time of adult eclosion. Parameters
related to reproductive table including gross reproductive rate, net
reproductive rate, intrinsic rate of natural increase and finite rate of
increase were 48.91, 37.63, 0.26 and 2.3, respectively. Mean age per
female/day, mean fertile egg per female/day, gross hatch rate, mean
net age fertility, mean net age fecundity, net fertility rate and net
fecundity rate were 2.23, 1.76, 0.87, 13.87, 14.26, 69.1 and 78.5,
respectively. Sex ratio of offspring also recorded daily. The highest
sex ratio for females was 0.88 in first day of oviposition. The sex
ratio decreased gradually and reached under 0.46 after the day 26 and
the oviposition rate declined. Then it seems that maintenance of
rearing culture of predatory thrips for mass rearing later than 26 days
after egg-laying commence is not profitable.
Abstract: Theoptimal extraction condition of dried Phaseolus
vulgaris powderwas studied. The three independent variables are raw
material concentration, shaking and centrifugaltime. The dependent
variables are both yield percentage of crude extract and alphaamylase
enzyme inhibition activity. The experimental design was
based on box-behnkendesign. Highest yield percentage of crude
extract could get from extraction condition at concentration of 1, 0,1,
concentration of 0.15 M ,extraction time for 2hour, and
separationtime for60 min. Moreover, the crude extract with highest
alpha-amylase enzyme inhibition activityoccurred by extraction
condition at concentration of 0.10 M, extraction time for 2 min, and
separation time for 45 min
Abstract: Sickle cell anemia is a recessive genetic disease
caused by the presence in the red blood cell, of abnormal hemoglobin
called hemoglobin S. It results from the replacement in the beta chain
of the acid glutamic acid by valin at position 6. Topics may be
homozygous (SS) or heterozygous (AS) most often
asymptomatic. Other mutations result in compound heterozygous:
- Synthesis of hemoglobin C mutation in the sixth leucin codon
(heterozygous SC);
- ß-thalassemia (heterozygous S-ß thalassemia).
SS homozygous, heterozygous SC and S- ß -thalassemia are grouped
under the major sickle cell syndromes.
To make a laboratory diagnosis of hemoglobinopathies in a
portion of the population in region of Batna, our study was
conducted on 115 patients with suspected sickle cell anemia, all cases
have benefited from hematological tests as blood count (count RBC,
calculated erythrocyte indices, MCV and MCHC, measuring the
hemoglobin concentration) and a biochemical test in this case
electrophoresis CAPILLARYS HEMOGLOBIN (E).
The results showed:
27 cases of sickle cell anemia were found on 115 suspected cases,
73,03% homozygous sickle cell disease and 59,25% sickle cell trait.
Finally, the double heterozygous S/C, represent the incidence rate of
3, 70%.
Abstract: This is a genetic comparison study of Arabian Oryx
(Oryx leucoryx) population at two different locations (A &B) based
on nuclear microsatellite DNA markers. Arabian Oryx is listed as
vulnerable and endanger by the World Conservation Union (IUCN).
Thirty microsatellite markers from bovine family were applied to
investigate the genetic diversity of the Arabian Oryx and to set up a
molecular inventory. Among 30 microsatellite markers used, 13
markers were moderately polymorphic. Arabian Oryx at location A
has shown better gene diversity over location B. However, mean
number of alleles were less than location B. Data of within
population inbreeding coefficient indicates inbreeding at both
locations (A&B). Based on the analysis of polymorphic microsatellite
markers, the study revealed that Arabian Oryx need a genetically
designed breeding program.
Abstract: Protein residue contact map is a compact
representation of secondary structure of protein. Due to the
information hold in the contact map, attentions from researchers in
related field were drawn and plenty of works have been done
throughout the past decade. Artificial intelligence approaches have
been widely adapted in related works such as neural networks,
genetic programming, and Hidden Markov model as well as support
vector machine. However, the performance of the prediction was not
generalized which probably depends on the data used to train and
generate the prediction model. This situation shown the importance
of the features or information used in affecting the prediction
performance. In this research, support vector machine was used to
predict protein residue contact map on different combination of
features in order to show and analyze the effectiveness of the
features.
Abstract: Changes in stem diameter of orchid plants were
investigated in a control growing climate. Previous studies have
focused on stem diameter in relation to plant water on terrestrial
plants in order to schedule the irrigation. The objective of this work
was to evaluate the ability of the strain gauges to capture changes in
the epiphytes plant stem. Experiments were carried out by using the
sympodial orchid, Dendrobium Sonia in a stressed condition. From
the findings, the sensor can detect changes in the plant stem and the
result can easily be used as a reference for further studies for the
development of a proper watering system.
Abstract: In the paper, the relative performances on spectral
classification of short exon and intron sequences of the human and
eleven model organisms is studied. In the simulations, all
combinations of sixteen one-sequence numerical representations, four
threshold values, and four window lengths are considered. Sequences
of 150-base length are chosen and for each organism, a total of
16,000 sequences are used for training and testing. Results indicate
that an appropriate combination of one-sequence numerical
representation, threshold value, and window length is essential for
arriving at top spectral classification results. For fixed-length
sequences, the precisions on exon and intron classification obtained
for different organisms are not the same because of their genomic
differences. In general, precision increases as sequence length
increases.
Abstract: The trial in the city, located 170 kilometers from the
Iranian city of Ahvaz was Omidiyeh. The main factor in this project
includes 4 levels in control (without hormones), use of hormones in
the seed, vegetative and flowering stage respectively. And sub-plots
included 3 varieties of vetch in three levels, with local names, was the
jewel in the study of light and Auxin in the vegetative and
reproductive different times in different varieties of vetch was
investigated. This test has been taken in the plots in a randomized
complete block with four replications. In order to study the effects of
the hormone Auxin in the growth stages (seed, vegetative and
flowering) to control (no hormone Auxin) on three local varieties of
vetch, the essence of light and plant height, number of pods per plant,
seed number The pods, seeds per plant, grain weight, grain yield,
plant dry weight and protein content were measured. Among the
vetch varieties for plant height, number of pods per plant, a seed per
plant, grain weight, grain yield, and plant dry weight and protein
levels of 1 percent of plant and seed number per pod per plant at 5%
level of There was no significant difference. Interactions for grain
yield per plant, grain yield and protein levels of 1 percent and the
number of seeds per pod and seed weight are significant differences
in levels 5 and plant height and plant dry weight of the interaction
were INFLUENCE There was no significant difference in them.
Abstract: The aims of this paper are to study the efficacy of
chitosan nanoparticles in stimulating specific antibody against
A/H1N1 influenza antigen in mice. Chitosan nanoparticles (CSN)
were characterized by TEM. The results showed that the average size
of CSN was from 80nm to 106nm. The efficacy of A/H1N1 influenza
vaccine loaded on the surface of CSN showed that loading efficiency
of A/H1N1 influenza antigen on CSN was from 93.75 to 100%. Safe
property of the vaccine were tested. In 10 days post vaccination,
group of CSN 30 kDa and 300 kDa loaded A/H1N1 influenza antigen
were the rate of immune response on mice to be 100% (9/9) higher
than Al(OH)3 and other adjuvant. 100% mice in the experiment of all
groups had immune response in 20 days post vaccination. The results
also showed that HI titer of the group using CSN 300 kDa as an
adjuvant increased significantly up to 3971 HIU, over three-fold
higher than the Al(OH)3 adjuvant, chitosan (CS), and one hundredfold
than the A/H1N1 antigen only. Stability of the vaccine
formulation was investigated.
Abstract: This study was set to determine the antimicrobial
activities of brine salting, chlorinated solution, and oil frying
treatments on enteric bacteria and fungi in Rastrineobola argentea
fish from fish landing beaches within L. Victoria basin of western
Kenya. Statistical differences in effectiveness of the different
treatment methods was determined by single factor ANOVA, and
paired two-tail t-Test was performed to compare the differences in
moisture contents before and after storage. Oil fried fish recorded the
lowest microbial loads, sodium chloride at 10% concentration was
the second most effective and chlorinated solution even at 150ppm
was the least effective against the bacteria and fungi in fish. Moisture
contents of the control and treated fish were significantly lower after
storage. These results show that oil frying of fish should be adopted
for processing and preserving Rastrineobola argentea which is the
most abundant and affordable fish species from Lake Victoria.
Abstract: The increasing usage of antibiotics in the animal
farming industry is an emerging worldwide problem contributing to
the development of antibiotic resistance. The purpose of this work was
to investigate the prevalence and antibiotic resistance profile of
bacterial isolates collected from aquatic environments and meats in a
peri-urban community in Daejeon, Korea. In an antibacterial
susceptibility test, the bacterial isolates showed a high incidence of
resistance (~ 26.04 %) to cefazolin, tetracycline, gentamycin,
norfloxacin, erythromycin and vancomycin. The results from a test for
multiple antibiotic resistance indicated that the isolates were
displaying an approximately 5-fold increase in the incidence of
multiple antibiotic resistance to combinations of two different
antibiotics compared to combinations of three or more antibiotics.
Most of the isolates showed multi-antibiotic resistance, and the
resistance patterns were similar among the sampling groups.
Sequencing data analysis of 16S rRNA showed that most of the
resistant isolates appeared to be dominated by the classes
Betaproteobacteria and Gammaproteobacteria in the phylum
Proteobacteria.
Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: Seaweed farming is emerging as a viable alternative
activity in the Indonesian fisheries sector. This paper aims to
investigate people-s perceptions of seaweed farming, to analyze its
social and economic impacts and to identify the problems and
obstacles hindering its continued development. Structured and
semi-structured questionnaires were prepared to obtain qualitative
data, and interviews were conducted with fishermen who also plant
seaweed. The findings showed that fishermen in the Laikang Bay were
enthusiastic about cultivating seaweeds and that seaweed plays a major
role in supporting the household economy of fishermen. However,
current seaweed drying technologies cannot support increased
seaweed production on a farm or plot, especially in the rainy season.
Additionally, variable monsoon seasons and long marketing channels
are still major constraints on the development of the industry. Finally,
capture fisheries, the primary economic livelihood of fishermen of
older generations, is being slowly replaced by seaweed farming.
Abstract: The present study was designed to demonstrate the seasonal variations in physico-chemical parameters of fish farm at Govt. Nursery Unit, Muzaffargarh, Department of Fisheries Govt. of Punjab, Pakistan for a period of eight months from January to August 2008. Water samples were collected on fifteen days basis and have been analyzed for estimation of Air temperature, Water temperature, Light penetration, pH, Total dissolved oxygen, Clouds, Carbonates, Bicarbonates, Total carbonates, Total dissolved solids, Chlorides, Calcium and Hardness. Seasonal fluctuations were observed in all the physico-chemical parameters of fish farm. The overall physicochemical parameters of fish pond water remained within the tolerable range throughout the study period.
Abstract: Most of the commercial gluten free products are
nutritionally inferior when compared to gluten containing
counterparts as manufacturers most often use the refined flours and
starches. So it is possible that people on gluten free diet have low
intake of fibre content. The foxtail millet flour and copra meal are
gluten free and have high fibre and protein contents. The formulation
of fibre rich gluten free cookies was optimized by response surface
methodology considering independent process variables as proportion
of Foxtail millet (Setaria italica) flour in mixed flour, fat content and
guar gum. The sugar, sodium chloride, sodium bicarbonates and
water were added in fixed proportion as 60, 1.0, 0.4 and 20% of
mixed flour weight, respectively. Optimum formulation obtained for
maximum spread ratio, fibre content, surface L-value, overall
acceptability and minimum breaking strength were 80% foxtail millet
flour in mixed flour, 42.8 % fat content and 0.05% guar gum.
Abstract: An ethnobotanical study was conducted to document
local knowledge and potentials of wild edible tubers that has been
reported and sighted and to investigate and record their distribution in
Pulau Redang and nearby islands of Terengganu, Malaysia.
Information was gathered from 42 villagers by using semi-structured
questionnaire. These respondents were selected randomly and no
appointment was made prior to the visits. For distribution, the
locations of wild edible tubers were recorded by using the Global
Positioning System (GPS). The wild edible tubers recorded were ubi
gadung, ubi toyo, ubi kasu, ubi jaga, ubi seratus and ubi kertas.
Dioscorea or commonly known as yam is reported to be one of the
major food sources worldwide. The majority of villagers used
Dioscorea hispida Dennst. or ubi gadung in many ways in their life
such as for food, medicinal purposes and fish poison. The villagers
have identified this ubi gadung by looking at the morphological
characteristics; that include leaf shape, stem and the color of the
tuber-s flesh.
Abstract: This study carried out to determine the effect of plant
densities on some agronomic characteristics of four safflower cultivars in spring planting. The experiment was conducted at Yazd,
Iran- using a factorial in a randomized complete block design with four replications. Cultivars were including Arak, IL, Asteria and Local and plant densities were 10, 13.3, 20 and 40 plant/m2. Number
of seeds/head, number of heads/plant, HI, 1000-seed weight and seed yield significantly decreased as planting density increased. With increasing planting density, LAI, plant height, first branch height and
biological yield increased. The highest seed yield was obtained in 13.3 plant/m2 (2167 kg/ha). There were significant differences
between cultivars. Local cv. had higher seed yield than the other cultivars mainly due to higher heads/plant and seeds/head.
Abstract: The common bean is the most important grain legume for direct human consumption in the world and BCMV is one of the world's most serious bean diseases that can reduce yield and quality of harvested product. To determine the best tolerance index to BCMV and recognize tolerant genotypes, 2 experiments were conducted in field conditions. Twenty five common bean genotypes were sown in 2 separate RCB design with 3 replications under contamination and non-contamination conditions. On the basis of the results of indices correlations GMP, MP and HARM were determined as the most suitable tolerance indices. The results of principle components analysis indicated 2 first components totally explained 98.52% of variations among data. The first and second components were named potential yield and stress susceptible respectively. Based on the results of BCMV tolerance indices assessment and biplot analysis WA8563-4, WA8563-2 and Cardinal were the genotypes that exhibited potential seed yield under contamination and noncontamination conditions.
Abstract: Soil chemical and physical properties have important
roles in compartment of the environment and agricultural
sustainability and human health. The objectives of this research is
determination of spatial distribution patterns of Cd, Zn, K, pH, TNV,
organic material and electrical conductivity (EC) in agricultural soils
of Natanz region in Esfehan province. In this study geostatistic and
non-geostatistic methods were used for prediction of spatial
distribution of these parameters. 64 composite soils samples were
taken at 0-20 cm depth. The study area is located in south of
NATANZ agricultural lands with area of 21660 hectares. Spatial
distribution of Cd, Zn, K, pH, TNV, organic material and electrical
conductivity (EC) was determined using geostatistic and geographic
information system. Results showed that Cd, pH, TNV and K data
has normal distribution and Zn, OC and EC data had not normal
distribution. Kriging, Inverse Distance Weighting (IDW), Local
Polynomial Interpolation (LPI) and Redial Basis functions (RBF)
methods were used to interpolation. Trend analysis showed that
organic carbon in north-south and east to west did not have trend
while K and TNV had second degree trend. We used some error
measurements include, mean absolute error(MAE), mean squared
error (MSE) and mean biased error(MBE). Ordinary
kriging(exponential model), LPI(Local polynomial interpolation),
RBF(radial basis functions) and IDW methods have been chosen as
the best methods to interpolating of the soil parameters. Prediction
maps by disjunctive kriging was shown that in whole study area was
intensive shortage of organic matter and more than 63.4 percent of
study area had shortage of K amount.