Abstract: In this study, static batch fermentation was used for bacterial cellulose production in date syrup solution (Bx. 10%) at 28°C using Gluconacetobacter. xylinus (PTCC 1734). The physicochemical properties of standard Sigma CMC and the produced carboxymethyl bacterial cellulose (CMBC) were studied using FT-IR spectroscopy, X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM). According to the FT-IR spectra the bands at 1664 and 1431 cm-1 indicate that carboxylic acid groups and carboxylate groups exist on the surface. The SEM imaging of CMBC and CMC carried out in magnification of 1K. Comparing the SEM imaging obviously showed that the ribbon shape in CMC remained but the length of ribbons became shorter while that shape changed to flake shape for CMBC. Determination of the area under XRD patterns demonstrated that the crystallinity amount of CMC was more than that for CMBC (51.08% and 81.84% for CMBC and CMC, respectively).
Abstract: Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value
Abstract: Agropyron cristatum L. Gaertn. is a native grass of
semiarid region in Iran which is quit resistant to cool and drought
climate and withstand heavy grazing. This species has close
phylogenetic relationship with Triticum and Hordeum. In this
research, the effect of seven different concentrations of growth
regulator 2,4-D on callus production and somatic embryogenesis of
A. cristatum was investigated on Murashige and Skoog medium. The
results showed that the rate of callus, embryo and neomorph were
highest in 1 mg L-1 2,4-D. Callus production was increased in 1 mg
L-1 2,4-D but dramatically decreased at 5.5 and 9 mg L-1 2,4-D. The
somatic embryos were observed at 1 and 4 mg L-1 2,4-D but matured
embryos and plantlet were only occurred at 1 mg L-1 2,4-D. There
were significant differences between 1 mg L-1 2,4-D and other
treatments for producing globular and torpedo embryos, plantlet,
rooted callus and number of roots (p
Abstract: Sixteen selected deep-sea fish obtained from Southern
Java Ocean and Western Sumatra Ocean was analyzed to determine
its proximate, fatty acid and mineral composition. The moisture
content was ranged from 64.38 to 86.04 %, ash from 0.17 to 0.69 %,
the fat content was 1.54 – 13.30 % while the protein content varied
from 15.84 to 23.60%. Among the fatty acids, oleic acid and palmitic
acid was the dominant MUFA and SFA. Linoleic acid was the
highest PUFA found at the selected deep-sea fish. Phospor was the
highest macroelement concentration on selected deep-sea fish,
followed by K, Ca, Mg and Iod, Fe and Zn among microelement. The
trace concentration was found at Se microelement.
Abstract: Fruit drying is a well known process mostly used for
preservation of fruits. Osmotic dehydration of apricot slices were
carried out in three different salt-sucrose concentrations and four
different temperatures. Also three different weight ratios of solution
to sample were conducted to one set of experiments. The dehydration
curves were constructed using Peleg-s model. Increasing the solution
volume increased the mass transfer rate and hence the solid gain
increased rapidly. Increasing the volume of osmotic media caused an
increase in overall mass transfer but a 'solution to sample' ratio of 5:1
gave the best product quality. The best temperature and concentration
that had a high water loss to solid gain ratio and an acceptable taste
were 40°C and 5%, respectively.
Abstract: The effect of magnetic field on germination
characteristics of two wheat Seeds has been studied under laboratory
conditions. Seeds were magnetically exposed to magnetic field
strengths, 125 or 250mT for different periods of time. Mean
germination time and the time required to obtain 10, 25, 50, 75 and
90%of seeds to germinate were calculated. The germination time for
each treatment were in general, higher than corresponding control
values, in the other word in treated seeds time required for mean seed
germination time increased nearly 3 hours in compared non treated
control seeds. T10 for doses D5, D6, D11 and D12 significantly higher
than the control values for both cultivars. Mean germination time
(MGT) in both cultivars significantly increased when the time of
seed exposed at magnetic field treatments increased , about 3 and 2
hour respectively for Omid and BCR cultivars.
Abstract: Nowadays, consumption of whole flours and flours
with high extraction rate is recommended, because of their high
amount of fibers, vitamins and minerals. Despite nutritional benefits
of whole flour, concentration of some undesirable components such
as phytic acid is higher than white flour. In this study, effect of
several lactic acid bacteria sourdough on Toast bread is investigated.
Sourdough from lactic acid bacteria (Lb. plantarum, Lb. reuteri) with
different dough yield (250 and 300) is made and incubated at 30°C
for 20 hour, then added to dough in the ratio of 10, 20 and 30%
replacement. Breads that supplemented with Lb. plantarum
sourdough had lower phytic acid. Higher replacement of sourdough
and higher DY cause higher decrease in phytic acid content.
Sourdough from Lb. plantarum, DY = 300 and 30% replacement
cause the highest decrease in phytic acid content (49.63 mg/100g).
As indicated by panelists, Lb. reuteri sourdough can present the
greatest effect on overall quality score of the breads. DY reduction
cause a decrease in bread quality score. Sensory score of Toast bread
is 81.71 in the samples that treated with Lb. reuteri sourdough with
DY = 250 and 20% replacement.
Abstract: In order to study of The Effect of seed inoculation
with Pseudomonas putida+Bacillus lentus on yield and yield
components of wheat (Triticum aestivum L.) cultivars, an experiment
was carried out as factorial based on Randomized Complete Block
Design (RCBD) in Agricultural Research Station of Shahrood
University of Technology. Results showed that inoculation with
Pseudomonas putida+Bacillus lentus promoted seed germination.
Also, inoculation with Pseudomonas putida+Bacillus lentus
significantly affected grain yield, Number of spikes per m2,
Number of grain per spike and 1000-seed weight and There was not
statistically significant difference between Chamran and Pishtaz
cultivars . Finally, the dosages of chemical fertilizers currently
applied in commercial wheat field in Iran (Shahrood region) could be
reduced through proper combination of Pseudomonas
putida+Bacillus lentus inoculation plus fertilization.
Abstract: Twenty - nine Holstein cows were used to evaluate the effects of different dry period (DP) lengths on milk yield and composition, some blood metabolites, and complete blood count (CBC). Cows were assigned to one of 2 treatments: 1) 60-d dry period, 2) 35-d DP. Milk yield, from calving to 60 days, was not different for cows on the treatments (p =0.130). Cows in the 35-d DP produced more milk protein and SNF compare with cows in treatment 1 (p ≤ 0.05). Serum glucose, non-esterified fatty acids (NEFA), beta hydroxyl butyrate acid (BHBA), blood urea nitrogen (BUN), urea, and glutamic oxaloacetic transaminase (GOT) were all similar among the treatments. Body condition score (BCS), body weight (BW), complete blood count (CBC) and health problems were similar between the treatments. The results of this study demonstrated we can reduce the dry period length to 35 days with no problems.
Abstract: In Croatia, the majority of cultured marine fish species are reared in net cages. The intensive production of the fish in net cages may generate the considerable amount of bio waste and change water quality especially in enclosed and semi-enclosed coastal areas. The aim of this paper is to assess the potential impact of sea bass (Dicentrarchus labrax L.) cage farm on water quality. The weak relationship between food supply and water quality parameters (nutrient content and phytoplankton biomass) was found, but significant changes in oxygen saturation was observed in the cages during the warmer period of a year especially in the morning (occasionally it dropped below 70 %). Despite of, satisfactory results of water quality parameters, it is necessary to establish comprehensive monitoring process, especially to include quality assessment of fouling communities.
Abstract: In this study four Holstein steers with rumen fistula
fed 7 kg of dry matter (DM) of diets differing in concentrate to
alfalfa hay ratios as 60:40, 70:30, 80:20, and 90:10 in a 4 × 4 latin
square design. The pH of the ruminal fluid was measured before
the morning feeding (0.0 h) to 8 h post feeding. In this study, a
two-layered feed-forward neural network trained by the
Levenberg-Marquardt algorithm was used for modelling of ruminal
pH. The input variables of the network were time, concentrate to
alfalfa hay ratios (C/F), non fiber carbohydrate (NFC) and neutral
detergent fiber (NDF). The output variable was the ruminal pH.
The modeling results showed that there was excellent agreement
between the experimental data and predicted values, with a high
determination coefficient (R2 >0.96). Therefore, we suggest using
these model-derived biological values to summarize continuously
recorded pH data.
Abstract: To assess the effects of functional protein on osteoblast, Large quantity of high-purity osteoblasts had been cultivated successfully by adopting sequential enzyme digestion. The growth curve of osteoblasts was protracted by cell counting. Proliferation of osteoblasts was assessed by MTT colorimetry. The experimental results show the functional protein can enhance proliferation, the properties of adhesion and discuss the effect of osteopontin on osteoblast.
Abstract: In the present study, we aimed to design the
intrauterine and extrauterine exposure to 1800 MHz GSM-like RF
radiation and investigate its possible bio-effects on infant female
rabbits. Totally thirty-six New Zealand White female rabbits, onemonth
old, were randomly divided into four groups which are
composed of 9 rabbits; i. Group I [Intrauterine (IU) exposure(-);
Extrauterine (EU) exposure (-)], Group II [IU exposure (-); EU
exposure (+)], Group III [IU exposure(+);EU exposure(-)], Group IV
[IU exposure (+);EU exposure(+)]. The master regulatory enzymes
activities of pentose phosphate pathway (glucose-6-phosphate
dehydrogenase, G-6PD; 6-phosphogluconate dehydrogenase, 6-
PGDH) and glutathione-dependent metabolism (glutathione
peroxidase, GSH-Px; glutathione reductase, GR; glutathione Stransferase,
GST, thioredoxin reductase, TRx) were analyzed in liver
tissues of young female rabbits. Decreased G-6PD, 6-PGD, GSH-Px,
GR activities were found in Group III compared to Group I (p
Abstract: Dietary macro and micro nutrients in their respective proportion and fractions present a practical potential tool to fabricate milk constituents since cells of lactating mammary glands obtain about 80 % of milk synthesis nutrients from blood, reflecting the existence of an isotonic equilibrium between blood and milk. Diverting milk biosynthetic activities through manipulation of nutrients towards producing milk not only keeping in view its significance as natural food but also as food item which prevents or dilutes the adverse effects of some diseases (like cardiovascular problem by saturated milk fat intake) has been area of interest in the last decade. Nutritional modification / supplementation has been reported to enhance conjugated linoleic acid, fatty acid type and concentration, essential fatty acid concentration, vitamin B12& C, Se, Cu, I and Fe which are involved to counter the health threats to human well being. Synchronizing dietary nutrients aimed to modify rumen dynamics towards synthesis of nutrients or their precursors to make their drive towards formulated milk constituents presents a practical option. Formulating dietary constituents to design milk constituents will let the farmers, consumers and investors know about the real potential and profit margins associated with this enterprise. This article briefly recapitulates the ways and means to modify milk constituents keeping an eye on human health and well being issues, which allows milk to serve more than a food item.
Abstract: In order to study of hydropriming and halopriming on
germination and early growth stage of wheat (Triticum aestivum) an
experiment was carried out in laboratory of the Department of
Agronomy and Plant breeding, Shahrood University of Technology.
Seed treatments consisted of T1: control (untreated seeds), T2:
soaking in distilled water for 18 h (hydropriming). T3: soaking in -
1.2 MPa solution of CaSO4 for 36 h (halopriming). Germination and
early seedling growth were studied using distilled water (control) and
under osmotic potentials of -0.4, -0.8 and -1.2 MPa for NaCl and
polyethylene glycol (PEG 6000), respectively. Results showed that
Hydroprimed seeds achieved maximum germination seedling dry
weight, especially during the higher osmotic potentials. Minimum
germination was recorded at untreated seeds (control) followed by
osmopriming. Under high osmotic potentials, hydroprimed seeds had
higher GI (germination index) as compared to haloprimed or
untreated seeds. Interaction effect of seed treatment and osmotic
potential significantly affected the seedling vigour index (SVI).
Abstract: An effort to find out the smaller size of cuttings for propagation of Morus alba was made in experimental area Department of Forestry, Range Management and Wildlife, University of Agriculture, Faisalabad, Pakistan. Different size of cuttings i.e. 2", 4", 6" and 8" were planted in polythene tubes of 3.5"x7". The effort was also made to compare the performance of cuttings in open air and in polythene low tunnel. Root length, number of root branches, root diameter and root fresh and dry weight were found maximum in two inches cuttings while minimum in four inches cuttings. Root growth was found maximum in open air as compared to under polythene sheet.
Abstract: A rare phenomenon of SDS-induced activation of a latent protease activity associated with the purified silkworm excretory red fluorescent protein (SE-RFP) was noticed. SE-RFP aliquots incubated with SDS for different time intervals indicated that the protein undergoes an obligatory breakdown into a number of subunits which exhibit autoproteolytic (acting upon themselves) and/or heteroproteolytic (acting on other proteins) activities. A strong serine protease activity of SE-RFP subunits on Bombyx mori nucleopolyhedrovirus (BmNPV) polyhedral protein was detected by zymography technique. A complete inhibition of BmNPV infection to silkworms was observed by the oral administration assay of the SE-RFP. Here, it is proposed that the SE-RFP prevents the initial infection of BmNPV to silkworms by obliterating the polyhedral protein. This is the first report on a silkworm red fluorescent protein that exhibits a protease activity on exposure to SDS. The present studies would help in understanding the antiviral mechanism of silkworm red fluorescent proteins.
Abstract: Matrix metalloproteinases (MMP) are a class of
structural and functional related enzymes involved in altering the
natural elements of the extracellular matrix. Most of the MMP
structures are cristalographycally determined and published in
WorldWide ProteinDataBank, isolated, in full structure or bound to
natural or synthetic inhibitors. This study proposes an algorithm to
replace missing crystallographic structures in PDB database. We
have compared the results of a chosen docking algorithm with a
known crystallographic structure in order to validate enzyme sites
reconstruction there where crystallographic data are missing.
Abstract: Bacterial cellulose, a biopolysaccharide, is produced by the bacterium, Gluconacetobacter xylinus. Static batch fermentation for bacterial cellulose production was studied in sucrose and date syrup solutions (Bx. 10%) at 28 °C using G. xylinus (PTCC, 1734). Results showed that the maximum yields of bacterial cellulose (BC) were 4.35 and 1.69 g/l00 ml for date syrup and sucrose medium after 336 hours fermentation period, respectively. Comparison of FTIR spectrum of cellulose with BC indicated appropriate coincidence which proved that the component produced by G. xylinus was cellulose. Determination of the area under X-ray diffractometry patterns demonstrated that the crystallinity amount of cellulose (83.61%) was more than that for the BC (60.73%). The scanning electron microscopy imaging of BC and cellulose were carried out in two magnifications of 1 and 6K. Results showed that the diameter ratio of BC to cellulose was approximately 1/30 which indicated more delicacy of BC fibers relative to cellulose.
Abstract: The study of the fouling deposition of pink guava
juice (PGJ) is relatively new research compared to milk fouling
deposit. In this work, a new experimental set-up was developed to
imitate the fouling formation in heat exchanger, namely a continuous
flow experimental set-up heat exchanger. The new experimental setup
was operated under industrial pasteurization temperature of PGJ,
which was at 93°C. While the flow rate and pasteurization period
were based on the experimental capacity, which were 0.5 and 1
liter/min for the flow rate and the pasteurization period was set for 1
hour. Characterization of the fouling deposit was determined by
using various methods. Microstructure of the deposits was carried
out using ESEM. Proximate analyses were performed to determine
the composition of moisture, fat, protein, fiber, ash and carbohydrate
content. A study on the hardness and stickiness of the fouling deposit
was done using a texture analyzer. The presence of seedstone in pink
guava juice was also analyzed using a particle analyzer. The findings
shown that seedstone from pink guava juice ranging from 168 to
200μm and carbohydrate was found to be a major composition
(47.7% of fouling deposit consists of carbohydrate). Comparison
between the hardness and stickiness of the deposits at two different
flow rates showed that fouling deposits were harder and denser at
higher flow rate. Findings from this work provide basis knowledge
for further study on fouling and cleaning of PGJ.