Effect of COD Loading Rate on Hydrogen Production from Alcohol Wastewater
The objective of this study was to investigate hydrogen production from alcohol wastewater by anaerobic sequencing batch reactor (ASBR) under thermophillic operation. The ASBR unit used in this study had a liquid holding volume of 4 L and was operated at 6 cycles per day. The seed sludge taken from an upflow anaerobic sludge blanket unit treating the same wastewater was boiled at 95 °C for 15 min before being fed to the ASBR unit. The ASBR system was operated at different COD loading rates at a thermophillic temperature (55 °C), and controlled pH of 5.5. When the system was operated under optimum conditions (providing maximum hydrogen production performance) at a feed COD of 60 000 mg/l, and a COD loading rate of 68 kg/m3 d, the produced gas contained 43 % H2 content in the produced gas. Moreover, the hydrogen yield and the specific hydrogen production rate (SHPR) were 130 ml H2/g COD removed and 2100 ml H2/l d, respectively.
[1] Das D, Veziroglu TN. "Hydrogen production by biological processes: a
survey of literature". International Journal Hydrogen Energy, Vol. 26,
pp. 13-28, 2001.
[2] Lay JJ, Le YJ, Noike T. "Feasibility of biological hydrogen production
from organic fraction of municipal solid waste". Water Research, Vol.
33, pp. 2579-2586, 1999.
[3] Mizuno O, Dinsdale R, Hawkes DL, Noike T. "Enhancement of
hydrogen production from glucose by nitrogen gas sparging".
Bioresource Technology, Vol. 73, pp.59-65, 2000.
[4] Kapdan IK, Kargi F. "Bio-hydrogen production from waste materials".
Enzyme Microbial Technology, Vol. 38, pp. 569-582, 2006.
[5] Mohan SV, Babu VL, Sarma PN. "Anaerobic biohydrogen production
from dairy wastewater treatment in sequencing batch reactor (AnSBR):
effect of organic loading rate". Enzyme Microbial Technology, Vol. 41,
pp. 506-15, 2007.
[6] Han SK, Shin HS. "Biohydrogen production by anaerobic fermentation
of food waste". International Journal Hydrogen Energy, Vol. 29pp. 569-
577, 2004.
[7] Sreethawong T, Chatsiriwatana S, Rangsunvigit P, Chavadej S.
"Hydrogen production from cassava wastewater using an aerobic
sequencing batch reactor: Effects of operational parameters, COD:N
ratio, and organic acid composition". International Journal Hydrogen
Energy, Vol. 35, pp. 4092-4102, 2010.
[8] Shin HS, Youn JH, Kim SH. "Hydrogen production from food waste in
anaerobic mesophillic and thermophillic acidogenesis". International
Journal Hydrogen Energy, Vol. 29, pp. 1355-1363. 2004.
[9] Arooj MF, Han SK, Kim AH, Kim DH, Shin HS. "Effect of HRT on
ASBR converting starch into biologicl hydrogen". International Journal
Hydrogen Energy, Vol. 33, pp. 6509-6514, 2008.
[10] Argun H, Kargi F, Kapdan IK, Oztekin R. "Biohydrogen production
by dark fermemtation of wheat powder solution: Effects of C/N and C/P
ratio on hydrogen yield and formation rate". International Journal
Hydrogen Energy, Vol. 33, pp. 1813-1819, 2008.
[11] Chong ML, Sabaratnam V, Shirai Y, Hassan MA. "Biohydrogen
production from biomass and industrial wastes by dark fermentation".
International Journal Hydrogen Energy, Vol. 34, pp. 3277-3287, 2009.
[12] Lee KS, Hsu YF, Lo YC, Lin PJ, Lin CY, Chang JS. "Exploring
optimal environmental factors for fermentative hydrogen production
from starch using mixed anaerobic microflora". International Journal
Hydrogen Energy, Vol. 32, pp. 1565-1572, 2008.
[13] Wang J, Wan W. "Comparison of different pretreatment methods
for enriching hydrogen-producing bacteria from digested sludge".
International Journal Hydrogen Energy, Vol. 33, pp. 2934-2941, 2008.
[14] Hawkes FR, Hussy I, Kyazze G, Dinsdale R, Hawkes DL. Continuous
dark fermentative hydrogen production by mesophilicmicroflora:
Principles and progress. International Journal Hydrogen Energy, Vol.
32, pp. 174-184, 2007.
[15] Lin CY, Hung WC. "Enhancement of fermentative hydrogen/ethanol
production from cellulose using mixed anaerobic cultures". International
Journal Hydrogen Energy, Vol. 33, pp. 3660-3667, 2008.
[16] Kapdan IK, Kargi F. "Bio-hydrogen production from waste materials".
Enzyme Microbial Technology, pp. 38569-53882, 2006.
[17] Cheng J, Su H, Zhou J, Song W, Cen K. "Hydrogen production by
mixed bacteria through dark and photo fermentation". International
Journal Hydrogen Energy, Vol. 36, pp. 450-457, 2011.
[18] Hwang JH, Choi JA, Shanab RA, Bhatnagar A, Min B, Song H, Kumar
E, Choi J, Lee ES, Kim YJ, Umg S, Lee DS, Joen BH. "Effect of pH and
sulfate concentration on hydrogen production using anaerobic mixed
microflora". International Journal Hydrogen Energy, Vol. 34, pp. 9702-
9719, 2009.
[19] Eaton AD, Clesceri LS, Rice EW, Greenberg AE, "Standard Methods
for The Examination of Water and Wastewater". American Public
Health Association (APHA), American Water Works Association
(AWWA) & Water Environment Federation (WEF), Washington, DC,
2005.
[20] Sreethawong T, Niyamapa T, Neramitsuk H, Rangsunvigit P,
Leethochawalit M, Chavadej S. "Hydrogen production from glucosecontaining
wastewater using an anaerobic sequencing batch reactor:
Effects of COD loading rate, nitrogen content. And organic acid
composition". Chemical Engineering Journal, Vol. 160, pp. 322-332,
2010.
[21] Fang HH, Liu H. "Effect of pH on hydrogen production from glucose by
a mixed culture". Bioresource Technology, Vol. 82, pp. 87-93, 2002.
[22] Lin CY, Lay CH. "Effects of carbonate and phosphate concentrations on
hydrogen production using anaerobic sewage sludge microflora".
International Journal Hydrogen Energy, Vol. 29, pp. 275-281, 2004.
[23] Chen WH, Sung S, Chen SY. "Biological hydrogen production in an
anaerobic sequencing batch reactor: pH and cyclic duration effects".
International Journal Hydrogen Energy, Vol. 34, pp. 227-234, 2009.
[24] Miyake J. "The science of biohydrogen: an energetic view. In: Zaborsky
OR, editor. Biohydrogen". New York: Plenum Press, pp. 7-18, 1998.
[25] Luo G, Xie L, Zou Z, Zhou Q, Wang JY. "Fermentative hydrogen
production from cassava stillage by mixed anaerobic microflora: Effects
of temperature and pH". Applied Energy, Vol. 87, pp. 3710-3117,
2010.
[26] O-Thong S, Prasertsan P, Intrasungkha, Dhamwichukorn S, Birkeland
NK. "Optimization of simultaneous thermophillic fermentative
hydrogen production and COD reduction from palm oil mill effluent by
Thermoanaerobacterium-rich sludge". International Journal Hydrogen
Energy, Vol. 33, pp. 1221-31, 2008.
[27] Hawkes FR, Dinsdale R, awkes DL, Hussy I. "Sustainable fermentative
hydrogen production: challenges for process optimization". International
Journal Hydrogen Energy, Vol. 27, pp. 1339-1347, 2002.
[28] Guo WQ, Ren NQ, Wang XJ, Xiang WS, Meng ZH, Ding J, Qu YY,
Zhang LS. "Biohydrogen production from ethanol-type fermentation of
molasses in an expanded granular sludge bed (EGSB) reactor".
International Journal Hydrogen Energy, Vol. 33, pp. 4981-4988, 2008.
[1] Das D, Veziroglu TN. "Hydrogen production by biological processes: a
survey of literature". International Journal Hydrogen Energy, Vol. 26,
pp. 13-28, 2001.
[2] Lay JJ, Le YJ, Noike T. "Feasibility of biological hydrogen production
from organic fraction of municipal solid waste". Water Research, Vol.
33, pp. 2579-2586, 1999.
[3] Mizuno O, Dinsdale R, Hawkes DL, Noike T. "Enhancement of
hydrogen production from glucose by nitrogen gas sparging".
Bioresource Technology, Vol. 73, pp.59-65, 2000.
[4] Kapdan IK, Kargi F. "Bio-hydrogen production from waste materials".
Enzyme Microbial Technology, Vol. 38, pp. 569-582, 2006.
[5] Mohan SV, Babu VL, Sarma PN. "Anaerobic biohydrogen production
from dairy wastewater treatment in sequencing batch reactor (AnSBR):
effect of organic loading rate". Enzyme Microbial Technology, Vol. 41,
pp. 506-15, 2007.
[6] Han SK, Shin HS. "Biohydrogen production by anaerobic fermentation
of food waste". International Journal Hydrogen Energy, Vol. 29pp. 569-
577, 2004.
[7] Sreethawong T, Chatsiriwatana S, Rangsunvigit P, Chavadej S.
"Hydrogen production from cassava wastewater using an aerobic
sequencing batch reactor: Effects of operational parameters, COD:N
ratio, and organic acid composition". International Journal Hydrogen
Energy, Vol. 35, pp. 4092-4102, 2010.
[8] Shin HS, Youn JH, Kim SH. "Hydrogen production from food waste in
anaerobic mesophillic and thermophillic acidogenesis". International
Journal Hydrogen Energy, Vol. 29, pp. 1355-1363. 2004.
[9] Arooj MF, Han SK, Kim AH, Kim DH, Shin HS. "Effect of HRT on
ASBR converting starch into biologicl hydrogen". International Journal
Hydrogen Energy, Vol. 33, pp. 6509-6514, 2008.
[10] Argun H, Kargi F, Kapdan IK, Oztekin R. "Biohydrogen production
by dark fermemtation of wheat powder solution: Effects of C/N and C/P
ratio on hydrogen yield and formation rate". International Journal
Hydrogen Energy, Vol. 33, pp. 1813-1819, 2008.
[11] Chong ML, Sabaratnam V, Shirai Y, Hassan MA. "Biohydrogen
production from biomass and industrial wastes by dark fermentation".
International Journal Hydrogen Energy, Vol. 34, pp. 3277-3287, 2009.
[12] Lee KS, Hsu YF, Lo YC, Lin PJ, Lin CY, Chang JS. "Exploring
optimal environmental factors for fermentative hydrogen production
from starch using mixed anaerobic microflora". International Journal
Hydrogen Energy, Vol. 32, pp. 1565-1572, 2008.
[13] Wang J, Wan W. "Comparison of different pretreatment methods
for enriching hydrogen-producing bacteria from digested sludge".
International Journal Hydrogen Energy, Vol. 33, pp. 2934-2941, 2008.
[14] Hawkes FR, Hussy I, Kyazze G, Dinsdale R, Hawkes DL. Continuous
dark fermentative hydrogen production by mesophilicmicroflora:
Principles and progress. International Journal Hydrogen Energy, Vol.
32, pp. 174-184, 2007.
[15] Lin CY, Hung WC. "Enhancement of fermentative hydrogen/ethanol
production from cellulose using mixed anaerobic cultures". International
Journal Hydrogen Energy, Vol. 33, pp. 3660-3667, 2008.
[16] Kapdan IK, Kargi F. "Bio-hydrogen production from waste materials".
Enzyme Microbial Technology, pp. 38569-53882, 2006.
[17] Cheng J, Su H, Zhou J, Song W, Cen K. "Hydrogen production by
mixed bacteria through dark and photo fermentation". International
Journal Hydrogen Energy, Vol. 36, pp. 450-457, 2011.
[18] Hwang JH, Choi JA, Shanab RA, Bhatnagar A, Min B, Song H, Kumar
E, Choi J, Lee ES, Kim YJ, Umg S, Lee DS, Joen BH. "Effect of pH and
sulfate concentration on hydrogen production using anaerobic mixed
microflora". International Journal Hydrogen Energy, Vol. 34, pp. 9702-
9719, 2009.
[19] Eaton AD, Clesceri LS, Rice EW, Greenberg AE, "Standard Methods
for The Examination of Water and Wastewater". American Public
Health Association (APHA), American Water Works Association
(AWWA) & Water Environment Federation (WEF), Washington, DC,
2005.
[20] Sreethawong T, Niyamapa T, Neramitsuk H, Rangsunvigit P,
Leethochawalit M, Chavadej S. "Hydrogen production from glucosecontaining
wastewater using an anaerobic sequencing batch reactor:
Effects of COD loading rate, nitrogen content. And organic acid
composition". Chemical Engineering Journal, Vol. 160, pp. 322-332,
2010.
[21] Fang HH, Liu H. "Effect of pH on hydrogen production from glucose by
a mixed culture". Bioresource Technology, Vol. 82, pp. 87-93, 2002.
[22] Lin CY, Lay CH. "Effects of carbonate and phosphate concentrations on
hydrogen production using anaerobic sewage sludge microflora".
International Journal Hydrogen Energy, Vol. 29, pp. 275-281, 2004.
[23] Chen WH, Sung S, Chen SY. "Biological hydrogen production in an
anaerobic sequencing batch reactor: pH and cyclic duration effects".
International Journal Hydrogen Energy, Vol. 34, pp. 227-234, 2009.
[24] Miyake J. "The science of biohydrogen: an energetic view. In: Zaborsky
OR, editor. Biohydrogen". New York: Plenum Press, pp. 7-18, 1998.
[25] Luo G, Xie L, Zou Z, Zhou Q, Wang JY. "Fermentative hydrogen
production from cassava stillage by mixed anaerobic microflora: Effects
of temperature and pH". Applied Energy, Vol. 87, pp. 3710-3117,
2010.
[26] O-Thong S, Prasertsan P, Intrasungkha, Dhamwichukorn S, Birkeland
NK. "Optimization of simultaneous thermophillic fermentative
hydrogen production and COD reduction from palm oil mill effluent by
Thermoanaerobacterium-rich sludge". International Journal Hydrogen
Energy, Vol. 33, pp. 1221-31, 2008.
[27] Hawkes FR, Dinsdale R, awkes DL, Hussy I. "Sustainable fermentative
hydrogen production: challenges for process optimization". International
Journal Hydrogen Energy, Vol. 27, pp. 1339-1347, 2002.
[28] Guo WQ, Ren NQ, Wang XJ, Xiang WS, Meng ZH, Ding J, Qu YY,
Zhang LS. "Biohydrogen production from ethanol-type fermentation of
molasses in an expanded granular sludge bed (EGSB) reactor".
International Journal Hydrogen Energy, Vol. 33, pp. 4981-4988, 2008.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:63130", author = "Patcharee Intanoo and Jittipan Chavadej and Sumaeth Chavadej", title = "Effect of COD Loading Rate on Hydrogen Production from Alcohol Wastewater", abstract = "The objective of this study was to investigate hydrogen production from alcohol wastewater by anaerobic sequencing batch reactor (ASBR) under thermophillic operation. The ASBR unit used in this study had a liquid holding volume of 4 L and was operated at 6 cycles per day. The seed sludge taken from an upflow anaerobic sludge blanket unit treating the same wastewater was boiled at 95 °C for 15 min before being fed to the ASBR unit. The ASBR system was operated at different COD loading rates at a thermophillic temperature (55 °C), and controlled pH of 5.5. When the system was operated under optimum conditions (providing maximum hydrogen production performance) at a feed COD of 60 000 mg/l, and a COD loading rate of 68 kg/m3 d, the produced gas contained 43 % H2 content in the produced gas. Moreover, the hydrogen yield and the specific hydrogen production rate (SHPR) were 130 ml H2/g COD removed and 2100 ml H2/l d, respectively.
", keywords = "Biohydrogen, Alcohol wastewater, Anaerobic
sequencing batch reactor (ASBR), Thermophillic operation", volume = "6", number = "4", pages = "375-5", }
