Abstract: The present investigation was aimed to develop methodology for the standardization of Marichyadi Vati and its raw materials. Standardization was carried using systematic Pharmacognostical and physicochemical parameters as per WHO guidelines. The detailed standardization of Marichyadi Vati, it is concluded that there are no major differences prevailed in the quality of marketed products and laboratory samples of Marichyadi Vati. However, market samples showed slightly better amount of Piperine than the laboratory sample by both methods. This is the first attempt to generate complete set of standards required for the Marichyadi Vati.
Abstract: The neurogenic potential of many herbal extracts used
in Indian medicine is hitherto unknown. Extracts derived from
Clitoria ternatea Linn have been used in Indian Ayurvedic system of
medicine as an ingredient of “Medhya rasayana", consumed for
improving memory and longevity in humans and also in treatment of
various neurological disorders. Our earlier experimental studies with
oral intubation of Clitoria ternatea aqueous root extract (CTR) had
shown significant enhancement of learning and memory in postnatal
and young adult Wistar rats. The present study was designed to
elucidate the in vitro effects of 200ng/ml of CTR on proliferation,
differentiation and growth of anterior subventricular zone neural
stem cells (aSVZ NSC-s) derived from prenatal and postnatal rat
pups. Results show significant increase in proliferation and growth of
neurospheres and increase in the yield of differentiated neurons of
aSVZ neural precursor cells (aSVZNPC-s) at 7 days in vitro when
treated with 200ng/ml of CTR as compared to age matched control.
Results indicate that CTR has growth promoting neurogenic effect on
aSVZ neural stem cells and their survival similar to neurotrophic
factors like Survivin, Neuregulin 1, FGF-2, BDNF possibly the basis
for enhanced learning and memory.
Abstract: There is a growing interest in the food industry and in preventive health care for the development and evaluation of natural antioxidants from medicinal plant materials. In the present work, extracts of three medicinal plants (Tilia argentea, Crataegi folium leaves and Polygonum bistorta roots) used in Turkish phytotheraphy were screened for their phenolic profiles and antioxidant properties. Crude extracts were obtained from different parts of plants, by solidliquid extraction with pure water, 70% acetone and 70% methanol aqueous solvents. The antioxidant activity of the extracts was determined by ABTS.+ radical cation scavenging activity. The Folin Ciocalteu procedure was used to assess the total phenolic concentrations of the extracts as gallic acid equivalents. A modified liquid chromatography-electro spray ionization-mass spectrometry (LC-ESI-MS) was used to obtain chromatographic profiles of the phenolic compounds in the medicinal plants. The predominant phenolic compounds detected in different extracts of the plants were catechin, protocatechuic and chlorogenic acids. The highest phenolic contents were obtained by using 70% acetone as aqueous solvent, whereas the lowest phenolic contents were obtained by water extraction due to Folin Ciocalteu results. The results indicate that acetone extracts of Tilia argentea had the highest antioxidant capacity as free ABTS radical scavengers. The lowest phenolic contents and antioxidant capacities were obtained from Polygonum bistorta root extracts.
Abstract: In this paper we study different similarity based approaches for the development of QSAR model devoted to the prediction of activity of antiobesity drugs. Classical similarity approaches are compared regarding to dissimilarity models based on the consideration of the calculation of Euclidean distances between the nonisomorphic fragments extracted in the matching process. Combining the classical similarity and dissimilarity approaches into a new similarity measure, the Approximate Similarity was also studied, and better results were obtained. The application of the proposed method to the development of quantitative structure-activity relationships (QSAR) has provided reliable tools for predicting of inhibitory activity of drugs. Acceptable results were obtained for the models presented here.
Abstract: Supplementation of palm vitamin E has been reported
to prevent loss of bone density in ovariectomised female rats. The
mechanism by which palm vitamin E exerts these effects is still
unknown. We hypothesized that palm vitamin E may act by
preventing the protein expression changes. Two dimensional poly
acyrilamide gel electrophoresis (2-D PAGE) and PD Quest software
genomic solutions Investigator (proteomics) was used to analyze the
differential protein expression profile in femoral and humeri bones
harvested from three groups of rats; sham-operated rats (SO),
ovariectomised rats (Ovx) and ovariectomised rats supplemented for
2 months with palm vitamin E. The results showed that there were
over 300 valued spot on each of the groups PVE and OVX as
compared to about 200 in SO. Comparison between the differential
protein expression between OVX and PVE groups showed that ten
spots were down –regulated in OVX but up-regulated in PVE. The
ten differential spots were separately named P1-P10. The
identification and understanding of the pathway of the differential
protein expression among the groups is ongoing and may account for
the molecular mechanism through which palm vitamin E exert its
anti-osteoporotic effect.
Abstract: In order to find the particular interaction energy
between cylcloguanil and the amino acids surrounding the pocket of
wild type and quadruple mutant type PfDHFR enzymes, the MP2
method with basis set 6-31G(d,p) level of calculations was
performed. The obtained interaction energies found that Asp54 has
the strongest interaction energy to both wild type and mutant type of -
12.439 and -11.250 kcal/mol, respectively and three amino acids;
Asp54, Ile164 and Ile14 formed the H-bonding with cycloguanil
drug. Importantly, the mutation at Ser108Asn was the key important
of cycloguanil resistant with showing repulsive interaction energy.
Abstract: The possible therapeutic effect of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated against acute hepatotoxicity induced by a single oral dose of acetaminophen (500mg/kg) in mice. Cannabidiol (two intraperitoneal injections, 5mg/kg, each) was given 1 hour and 12 hours following acetaminophen administration. Acetaminophen administration caused significant elevations of serum alanine aminotransferase, and hepatic malondialdehyde, and nitric oxide levels, and a significant decrease in hepatic reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters resulted from acetaminophen administration. Also, histopathological examination showed that cannabidiol markedly attenuated ameliorated acetaminophen-induced liver tissue damage. These results emphasize that cannabidiol represents a potential therapeutic option to protect against acetaminophen hepartotoxicity which is a common clinical problem.
Abstract: In this investigation, the antibacterial effects of
ethanolic and 7:3 isopropyl –hexane mixture extracts of Zingiber
officinale were evaluated against three Gram positive bacteria, B.
cereus, S.epidermidis, S. aureus and three Gram negative bacteria, E.
coli, K.pneumonia and P.areuginosa. Utilizing paper disk diffusion
and well methods in-vitro, MIC and MBC were determined by
macrodilution. The results showed that ethanolic rhizome extract of
ginger had significantly active than Isopropyl –hexan extract. Further
work needs to be done in these extracts including fractionation to
isolate active constituents and subsequent pharmacological
evaluation.
Abstract: New graph similarity methods have been proposed in this work with the aim to refining the chemical information extracted from molecules matching. For this purpose, data fusion of the isomorphic and nonisomorphic subgraphs into a new similarity measure, the Approximate Similarity, was carried out by several approaches. The application of the proposed method to the development of quantitative structure-activity relationships (QSAR) has provided reliable tools for predicting several pharmacological parameters: binding of steroids to the globulin-corticosteroid receptor, the activity of benzodiazepine receptor compounds, and the blood brain barrier permeability. Acceptable results were obtained for the models presented here.
Abstract: This study describes the methodology for the development of a validated in-vitro in-vivo correlation (IVIVC) for metoprolol tartrate modified release dosage forms with distinctive release rate characteristics. Modified release dosage forms were formulated by microencapsulation of metoprolol tartrate into different amounts of ethylcellulose by non-solvent addition technique. Then in-vitro and in-vivo studies were conducted to develop and validate level A IVIVC for metoprolol tartrate. The values of regression co-efficient (R2-values) for IVIVC of T2 and T3 formulations were not significantly (p
Abstract: A sensitive and specific method for quantitative
determination of aflatoxins(B1, B2, G1,G2), deoxynivalenol,
fumonisin(B1,B2), ochratoxin A, zearalenone, T-2 and HT-2 in
roasted and ground grains using liquid chromatography combined
with tandem mass spectrometry. A double extraction using a
phosphate buffer solution followed by methanol was applied to
achieve effective co extraction of 11 mycotoxins. A multitoxin
immunoaffinity column for all these mycotoxins was used to clean up
the extract. The LODs of mycotoxins were 0.1~6.1 μg/kg, LOQs were
0.3~18.4 μg/kg. Forty seven samples collected from Seoul (Korea) for
mycotoxin contamination monitoring. The results showed that the
occurrence of zearalenone and deoxynivalenol were frequent.
Zearalenone was detected in all samples and deoxynivalenol was
detected in 80.9 % samples in the range 0.626 ~ 29.264 μg/kg and N.D
~ 48.332 μg/kg respectively. Fumonisins and ochratoxin A were
detected in 46.8% samples and 17 % samples respectively, aflatoxins
and T-2/HT-2 toxins were not detected all samples.
Abstract: Acetaminophen (Paracetamol) tablets are popular OTC products among patients as analgesics and antipyretics. Paracetamol is marketed by a lot of suppliers around the world. The aim of the present investigation was to compare between many types of paracetamol tablets obtained from different suppliers (six brands produced by different pharmaceutical companies in middle east countries, and Panadol® manufactured in Ireland), by different quality control tests according to USP pharmacopeia.Using Non official tests-hardness and friability; official tests- disintegration, dissolution, and drug content. Additionally, evaluate the influence of temperatures 4°C, 25°C and 40°C at 75% relative humidity on the stability of the same brands in their original packaging has been conducted for two months. The results revealed that all paracetamol tablet brands complied with the official USP specifications. In conclusion, paracetamol tablets preferred to be stored at 25°C. All the tested brands being biopharmaceutically and chemically equivalent.
Abstract: Presence of phytosterol compound in Durian seed
(Durio zibethinus) or known as King of fruits has been discovered
from screening work using reagent test. Further analysis work has
been carried out using mass spectrometer in order to support the
priliminary finding. Isolation and purification of the major
phytosterol has been carried out using an open column
chromatography. The separation was monitored using thin layer
chromatography (TLC). Major isolated compounds and purified
phytosterol were identified using mass spectrometer and nuclear
magnetic resonance (NMR). This novel finding could promote
utilization of durian seeds as a functional ingredient in food products
through production of standardized extract based on phytosterol
content.
Abstract: The aim of the present study was to evaluate the
mucoadhesion and the release of nicotinamide gel formulations using
in vitro methods. An agar plate technique was used to investigate the
adhesiveness of the gels whereas a diffusion apparatus was employed
to determine the release of nicotinamide from the gels. In this
respect, 10% w/w nicotinamide gels containing bioadhesive
polymers: Carbopol 934P (0.5-2% w/w), hydroxypropylmethyl
cellulose (HPMC) (4-10% w/w), sodium carboxymethyl cellulose
(SCMC) (4-6% w/w) and methylcellulose 4000 (MC) (3-5% w/w)
were prepared. The gel formulations had pH values in the range of
7.14 - 8.17, which were considered appropriate to oral mucosa
application. In general, the rank order of pH values appeared to be
SCMC > MC4000 > HPMC > Carbopol 934P. Types and
concentrations of polymers used somewhat affected the
adhesiveness. It was found that anionic polymers (Carbopol 934 and
SCMC) adhered more firmly to the agar plate than the neutral
polymers (HPMC and MC 4000). The formulation containing 0.5%
Carbopol 934P (F1) showed the highest release rate. With the
exception of the formulation F1, the neutral polymers tended to give
higher relate rates than the anionic polymers. For oral tissue
treatment, the optimum has to be balanced between the residence
time (adhesiveness) of the formulations and the release rate of the
drug. The formulations containing the anionic polymers: Carbopol
934P or SCMC possessed suitable physical properties (appearance,
pH and viscosity). In addition, for anionic polymer formulations,
justifiable mucoadhesive properties and reasonable release rates of
nicotinamide were achieved. Accordingly, these gel formulations
may be applied for the treatment of oral mucosal lesions.
Abstract: The adverse effects of Clindamycin (Clind.) /
Ibuprofen (Ibu.) combination on liver, kidney, blood elements and the
significances of antioxidants (N-acetylcysteine and Zinc) against
these effects were evaluated. The study includes: Group I; control
n=30, Group II; patients on Clind.300mg/Ibu.400mg twice daily for a
week n=30, Group III; patients on Clind.300mg/Ibu.400mg+Nacetylcysteine
200mg twice daily for a week n=15 and Group IV;
patients on Clind.300mg/Ibu.400mg+Zinc50mg twice daily for a
week n=15. Serum malondialdehyde (MDA), alanine transferase
(ALT), aspartate transferase (AST), γ glutamyl transferase (GGT),
creatinine, blood urea nitrogen (BUN) were measured. Applying one
way ANOVA followed by Tuckey Kramer post test, Group II showed
significant increase in ALT, AST, GGT, BUN and decrease in Hb,
RBCs, platelets than Group I. Group III showed significant decrease
in ALT, AST, GGT, BUN than Group II. Moreover, Group IV
showed significant decrease in ALT, AST, GGT and increase in Hb,
RBCs, and platelets than Group II. Conclusively, Adding Zinc or Nacetylcysteine
buffer the oxidative stress and improve the therapeutic
outcome of Clindamycin/Ibuprofen combination.
Abstract: Transdermal delivery of ondansetron hydrochloride (OdHCl) can prevent the problems encountered with oral ondansetron. In previously conducted studies, effect of amount of polyvinyl pyrrolidone, permeation enhancer and casting solvent on the physicochemical properties on OdHCl were investigated. It is feasible to develop ondansetron transdermal patch by using ethyl cellulose and polyvinyl pyrrolidone with dibutyl pthalate as plasticizer, however, the desired flux is not achieved. The primary aim of this study is to use dimethyl succinate (DMS) and propylene glycol that are not incorporated in previous studies to determine their effect on the physicochemical properties of an OdHCl transdermal patch using ethyl cellulose and polyvinyl pyrrolidone. This study also investigates the effect of permeation enhancer (eugenol and phosphatidylcholine) on the release of OdHCl. The results showed that propylene glycol is a more suitable plasticizer compared to DMS in the fabrication of OdHCl transdermal patch using ethyl cellulose and polyvinyl pyrrolidone as polymers. Propylene glycol containing patch has optimum drug content, thickness, moisture content and water absorption, tensile strength, and a better release profile than DMS. Eugenol and phosphatidylcholine can increase release of OdHCl from the patches. From the physicochemical result and permeation profile, a combination of 350mg of ethyl cellulose, 150mg polyvinyl pyrrolidone, 3% of total polymer weight of eugenol, and 40% of total polymer weight of propylene glycol is the most suitable formulation to develop an OdHCl patch. OdHCl release did not increase with increasing the percentage of plasticiser. DMS 4, PG 4, DMS 9, PG 9, DMS 14, and PG 14 gave better release profiles where using 300mg: 0mg, 300mg: 100mg, and 350mg: 150mg of EC: PVP. Thus, 40% of PG or DMS appeared to be the optimum amount of plasticiser when the above combination where EC: PVP was used. It was concluded from the study that a patch formulation containing 350mg EC, 150mg PVP, 40% PG and 3% eugenol is the best transdermal matrix patch compositions for the uniform and continuous release/permeation of OdHCl over an extended period. This patch design can be used for further pharmacokinetic and pharmacodynamic studies in suitable animal models.
Abstract: The plant world is the source of many medicines.
Recently, researchers have estimated that there are approximately
400,000 plant species worldwide, of which about a quarter or a third
have been used by societies for medicinal purposes. The human uses
of plants for thousands of years to treat various ailments, in many
developing countries, much of the population trust in traditional
doctors and their collections of medicinal plants to treat them.
Essential oils have many therapeutic properties. In herbal medicine,
they are used for their antiseptic properties against infectious
diseases of fungal origin, against dermatophytes, those of bacterial
origin. The aim of our study is to determine the antimicrobial effect
of essential oils of the plant Trigonella focnum greacum on some
pathogenic bacteria, it is a medicinal plant used in traditional
therapy. The test adopted is based on the diffusion method on solid
medium (Antibiogram), this method determines the sensitivity or
resistance of a microorganism vis-à-vis the extract studied. Our study
reveals that the essential oil of the plant Trigonella focnum greacum
has a different effect on the resistance of germs. For staphiloccocus
Pseudomonnas aeroginosa and Krebsilla, are moderately sensitive
strains, also Escherichia coli and Candida albicans represents a high
sensitivity. By against Proteus is a strain that represents a weak
sensitivity.
Abstract: Recent years have instance that there is a invigoration
of interest in drug discovery from medicinal plants for the support of
health in all parts of the world . This study was designed to examine
the in vitro antimicrobial activities of the flowers and leaves
methanolic and ethanolic extracts of Chenopodium album L.
Chenopodium album Linn. flowers and leaves were collected from
East Esfahan, Iran. The effects of methanolic and ethanolic extracts
were tested against 4 bacterial strains by using disc,well-diffusion
method. Results showed that flowers and leaves methanolic and
ethanolic extracts of C.album don-t have any activity against the
selected bacterial strains. Our study has indicated that ,there are
effective different factors on antimicrobial properties of plant extracts
Abstract: Alpinia galanga is rhizome, generally known as
Greater galangal and is selected for isolation of newer constituents
accountable for various therapeutic activities. Present study is
intended to isolate glycoside from Alpinia galanga rhizomes. Alpinia
galanga methanolic extract was column chromatograph and eluted
with ethyl acetate-methanol (99:1) to isolate compound β-Sitosterol
Diarabinoside. Herein, the isolation and structural elucidation of new
compound is described. Chemical investigation of methanolic extract
of rhizomes of Alpinia galanga furnished a new compound β-
Sitosterol Diarabinoside. The IR, NMR and MASS investigations of
isolated compound confirmed its structure as β-Sitosterol
Diarabinoside, which is isolated for the first time from a medicinal
plant or any synthetic source.
Abstract: In article the data of acute toxicity for pre-clinical
researches of Ramon preparation is described. Ramon effects to
clinical characteristics of blood, cardio-vascular system, hepatotoxic
and diuretic effects were studied.