Abstract: Environmental micro-organisms include a large number of taxa and some species that are generally considered nonpathogenic, but can represent a risk in certain conditions, especially for elderly people and immunocompromised individuals. Chemotaxonomic identification techniques are powerful tools for environmental micro-organisms, and cellular fatty acid methyl esters (FAME) content is a powerful fingerprinting identification technique. A system based on an unsupervised artificial neural network (ANN) was set up using the fatty acid profiles of standard bacterial strains, obtained by gas-chromatography, used as learning data. We analysed 45 certified strains belonging to Acinetobacter, Aeromonas, Alcaligenes, Aquaspirillum, Arthrobacter, Bacillus, Brevundimonas, Enterobacter, Flavobacterium, Micrococcus, Pseudomonas, Serratia, Shewanella and Vibrio genera. A set of 79 bacteria isolated from a drinking water line (AMGA, the major water supply system in Genoa) were used as an example for identification compared to standard MIDI method. The resulting ANN output map was found to be a very powerful tool to identify these fresh isolates.
Abstract: Phylogenies ; The evolutionary histories of groups of
species are one of the most widely used tools throughout the life
sciences, as well as objects of research with in systematic,
evolutionary biology. In every phylogenetic analysis reconstruction
produces trees. These trees represent the evolutionary histories of
many groups of organisms, bacteria due to horizontal gene transfer
and plants due to process of hybridization. The process of gene
transfer in bacteria and hybridization in plants lead to reticulate
networks, therefore, the methods of constructing trees fail in
constructing reticulate networks. In this paper a model has been
employed to reconstruct phylogenetic network in honey bee. This
network represents reticulate evolution in honey bee. The maximum
parsimony approach has been used to obtain this reticulate network.
Abstract: Our results showed that for the growth of qualitative
seedling and vegetative raw material of ðó. marschallianus Willd. and
T. serphyllum L. it is more profitable to use the in vitro and
hydroponics combined method. In in vitro culture it is possible to do
micro-propagation whole year with 98-99% rhizogenesis. 30000
micro-plants were obtained from one explant during 9 months.
Hydroponic conditions provide the necessary microclimate for
microplants where the survival rate without acclimatization was
93.3%. The essential oil content in hydroponic dry herb of both
species in vegetative and blossom phase was 1.3% whereas in wild
plants it was 1.2%, the content of extractive substances and vitamin
C also exceeded wild plants. Our biochemical and radiochemical
investigations indicated that the medicinal raw materials obtained
from hydroponic and wild plants of Thymus species correspond to
the demands of SPh XI, and the content of artificial radionuclides
does not exceed the MACL.
Abstract: Many of the compounds present in potato are important because of their beneficial effects on health, therefore, are highly desirable in the human diet. Potato tubers contain significant amounts of anthocyanins. The aim of this research was to determine the content of anthocyanins and its relationship with the colour of organically and conventionally cultivated potato varieties. In the research eight potato samples of three potato varieties were analyzed on anthocyanins, dry matter content and color. Obtained results show that there was no significant influence on amount of anthocyanins between different cultivation environments (p>0.05) while between varieties – significant difference (p
Abstract: We report a novel fusion tag for expressing
recombinant proteins in E. coli. The fusion tag is the C-terminus part
of the human GMCSF gene comprising 45 amino acids, which aid in
over expression of otherwise non expressible genes. Expression of
hIFN a2b with this fusion tag also escapes the requirement of rare
codons for expression. This is also a first report of a small fusion tag
of human origin having affinity to heparin sepharose column
facilitating the purification of fusion protein.
Abstract: In this study, communities of ammonia-oxidizing
archaea (AOA) and ammonia-oxidizing bacteria (AOB) in nitrifying
activated sludge (NAS) prepared by enriching sludge from a
municipal wastewater treatment plant in three continuous-flow
reactors receiving an inorganic medium containing different
ammonium concentrations of 2, 10, and 30 mM NH4
+-N (NAS2,
NAS10, and NAS30, respectively) were investigated using molecular
analysis. Results suggested that almost all AOA clones from NAS2,
NAS10, and NAS30 fell into the same AOA cluster and AOA
communities in NAS2 and NAS10 were more diverse than those of
NAS30. In contrast to AOA, AOB communities obviously shifted
from the seed sludge to enriched NASs and in each enriched NAS,
communities of AOB varied particularly. The seed sludge contained
members of N. communis cluster and N. oligotropha cluster. After it
was enriched under various ammonium loads, members of N.
communis cluster disappeared from all enriched NASs. AOB with
high affinity to ammonia presented in NAS 2, AOB with low affinity
to ammonia presented in NAS 30, and both types of AOB survived in
NAS 10. These demonstrated that ammonium load significantly
influenced AOB communities, but not AOA communities in enriched
NASs.
Abstract: Organochlorine pesticides (OCPs) are known to be
persistent and bioaccumulative toxicants that may cause reproductive
impairments in wildlife as well as human. The current study uses the
snail-eating turtle Malayemys macrocephala, a long-lived animal
commonly distribute in rice field habitat in central part of Thailand,
as a sentinel to monitor OCP contamination in environment. The
nest soil, complete clutch of eggs, and blood of the turtle were
collected from agricultural areas in the Chao Phraya River Basin,
Thailand during the nesting season of 2007-2008. The novel
methods for tissue extraction by an accelerated solvent extractor
(ASE, for egg) and liquid-liquid extraction (for blood) have been
developed. The nineteen OCP residues were analyzed by gas
chromatography with micro-electron captured detector (GC-μECD).
The validated methods have met requirements of the AOAC
standard. The results indicated that significant amounts of OCPs are
still contaminated in nest soil and eggs of the turtle even though the
OCPs had been banned in this area for many years. This suggested
the potential risk to health of wildlife as well as human in the area.
Abstract: P16/INK4A is tumor suppressor protein that plays a critical role in cell cycle regulation. Loss of P16 protein expression has been implicated in pathogenesis of many cancers, including lymphoma. Therefore, we sought to investigate if loss of P16 protein expression is associated with lymphoma and/or any specific lymphoma subtypes (Hodgkin-s lymphoma (HL) and nonHodgkin-s lymphoma (NHL)). Fifty-five lymphoma cases consisted of 30 cases of HL and 25 cases of NHL, with an age range of 3 to 78 years, were examined for loss of P16 by immunohistochemical technique using a specific antibody reacting against P16. In total, P16 loss was seen in 33% of all lymphoma cases. P16 loss was identified in 47.7% of HL cases. In contrast, only 16% of NHL showed loss of P16. Loss of P16 was seen in 67% of HL patients with 50 years of age or older, whereas P16 loss was found in only 42% of HL patients with less than 50 years of age. P16 loss in HL is somewhat higher in male (55%) than in female (30%). In subtypes of HL, P16 loss was found exclusively in all cases of lymphocyte depletion, lymphocyte predominance and unclassified cases, whereas P16 loss was seen in 39% of mixed cellularity and 29% of nodular sclerosis cases. In low grade NHL patients, P16 loss was seen in approximately one-third of cases, whereas no or very rare of P16 loss was found in intermediate and high grade cases. P16 loss did not show any correlation with age or gender of NHL patients. In conclusion, the high rate of P16 loss seen in our study suggests that loss of P16 expression plays a critical role in the pathogenesis of lymphoma, particularly with HL.
Abstract: The aim of a biological model is to understand the
integrated structure and behavior of complex biological systems as a
function of the underlying molecular networks to achieve simulation
and forecast of their operation. Although several approaches have
been introduced to take into account structural and environment
related features, relatively little attention has been given to represent
the behavior of biological systems. The Abstract Biological Process
(ABP) model illustrated in this paper is an object-oriented model
based on UML (the standard object-oriented language). Its main
objective is to bring into focus the functional aspects of the
biological system under analysis.
Abstract: Identifying protein coding regions in DNA sequences is a basic step in the location of genes. Several approaches based on signal processing tools have been applied to solve this problem, trying to achieve more accurate predictions. This paper presents a new predictor that improves the efficacy of three techniques that use the Fourier Transform to predict coding regions, and that could be computed using an algorithm that reduces the computation load. Some ideas about the combination of the predictor with other methods are discussed. ROC curves are used to demonstrate the efficacy of the proposed predictor, based on the computation of 25 DNA sequences from three different organisms.
Abstract: Plant tissue culture is an important in vitro technology applied for agricultural and industrial production. A sterile condition of culture medium is one of the main aspects. The alternative technique for medium sterilization to replace autoclaving was carried out. For sterilization of plant tissue culture medium without autoclaving, ten commercial pure essential oils and 5 disinfectants were tested. Each essential oil or disinfectant was added to a 20-mL Murashige and Skoog (MS) medium before medium was solidified in a 120-mL container, kept for 2 weeks before evaluating sterile conditions. Treated media, supplemented with essential oils or disinfectants, were compared to control medium, autoclaved at 121 degree Celsius for 15 min. Sterile conditions of MS medium were found 100% from betel oil or clove oil (18 mL/20 mL medium), cinnamon oil (36 mL/20 mL medium), lavender oil or holy basil oil (108 mL/20 mL medium), and lemon oil or tea tree oil or turmeric oil (252 mL/20 mL medium), compared to 100% sterile condition from autoclaved medium. For disinfectants, 2% iodine + 2.4% potassium iodide, 2% merbromine solution, 10% povidone-iodine, 6% sodium hypochlorite or 0.1% thimerosal at 36 mL/20 mL medium provided 100% sterile conditions. Furthermore, growth of new shoots from chrysanthemum node explants on treated media (fresh weight, shoot length, root length and number of node) were also reported and discussed in the comparison of those on autoclaved medium.
Abstract: 15 strains of oil-destructing microorganisms were
isolated from oil polluted soil of Western Kazakhstan. Strains 2-A
and 41-3 with the highest oil-destructing activities were chosen from
them. It was shown that these strains oxidized n-alkanes very well,
but isoalkanes, isoparaffin, cycloparaffin and heavy aromatic
compounds were destructed very slowly. These both strains were
tested as preparations for bioremediation of oil-polluted soil in model
and field experiments. The degree of utilizing of soil oil by this
preparation was 79-84 % in field experiments.
Abstract: There is a world-wide need for the development of sustainable management strategies to control pest infestation and the development of phosphine (PH3) resistance in lesser grain borer (Rhyzopertha dominica). Computer simulation models can provide a relatively fast, safe and inexpensive way to weigh the merits of various management options. However, the usefulness of simulation models relies on the accurate estimation of important model parameters, such as mortality. Concentration and time of exposure are both important in determining mortality in response to a toxic agent. Recent research indicated the existence of two resistance phenotypes in R. dominica in Australia, weak and strong, and revealed that the presence of resistance alleles at two loci confers strong resistance, thus motivating the construction of a two-locus model of resistance. Experimental data sets on purified pest strains, each corresponding to a single genotype of our two-locus model, were also available. Hence it became possible to explicitly include mortalities of the different genotypes in the model. In this paper we described how we used two generalized linear models (GLM), probit and logistic models, to fit the available experimental data sets. We used a direct algebraic approach generalized inverse matrix technique, rather than the traditional maximum likelihood estimation, to estimate the model parameters. The results show that both probit and logistic models fit the data sets well but the former is much better in terms of small least squares (numerical) errors. Meanwhile, the generalized inverse matrix technique achieved similar accuracy results to those from the maximum likelihood estimation, but is less time consuming and computationally demanding.
Abstract: Experiments were carried out on the survival and growth of Rasbora daniconius, Puntius ticto and Puntius conchonius. The motivation of the study was to obtain information for growing the fish on a commercial scale for their use as biological control agents against mosquito larvae. The effects of temperature, total hardness, DO, pH and feed on the growth of fish were also investigated. Excessive value of total hardness was found because very rich calcium ion is present in Chitrakoot area. There was significant increases in growth rates of fish as temperature was increased from 280C to 300C. Further increases in temperature up to 320C, did not further affect growth. The positive and highly significant correlations 0.991488, 0.9581 and 0.9935 were found between length and weight of P. ticto, P. conchonius and R. daniconius respectively. The regression was significant at 5% level of probability.
Abstract: Neem is a highly heterozygous and commercially
important perennial plant. Conventionally, it is propagated by seeds
which loose viability within two weeks. Strictly cross pollinating
nature of the plant causes serious barrier to the genetic improvement
by conventional methods. Alternative methods of tree improvement
such as somatic hybridization, mutagenesis and genetic
transformation require an efficient in vitro plant regeneration system.
In this regard, somatic embryogenesis particularly secondary somatic
embryogenesis may offer an effective system for large scale plant
propagation without affecting the clonal fidelity of the regenerants. It
can be used for synthetic seed production, which further bolsters
conservation of this tree species which is otherwise very difficult
The present report describes the culture conditions necessary to
induce and maintain repetitive somatic embryogenesis, for the first
time, in neem. Out of various treatments tested, the somatic embryos
were induced directly from immature zygotic embryos of neem on
MS + TDZ (0.1 μM) + ABA (4 μM), in more than 76 % cultures.
Direct secondary somatic embryogenesis occurred from primary
somatic embryos on MS + IAA (5 μM) + GA3 (5 μM) in 12.5 %
cultures. Embryogenic competence of the explant as well as of the
primary embryos was maintained for a long period by repeated
subcultures at frequent intervals. A maximum of 10 % of these
somatic embryos were converted into plantlets.
Abstract: Retinoic acid is like a steroid hormone that plays a
role in embryo formation, proliferation of spermatogonia cells,
ephitelial cells differentiation and organogenesis. Retinoic acid can
influences seminiferous tubule formation during embryonic testis
development and also play a role in the regulation of ovarian function
and female reproductive tract by suppressing the hormones FSH
receptor expression. The excessive use of retinoic acid caused
abnormalities in the fetus. The result showed that there is the
influence of retinoic acid on the developmet of mice fetal testes, for
examples disruption of the formation of seminiferous tubules and
tubules seemed to be hollow, spermatogonia cells are relatively few
in number and caused Leydig cells count relatively more. While in
the female fetus does not caused the formation of primordial follicles
and disrupted the development of germinal ephitelial cells of fetal
ovaries of female mice (mus musculus) Swiss Webster.
Abstract: Candida spp. are common and aggressive pathogens. Because of the growing resistance of Candida spp. to current antifungals, novel targets, found in Candida spp. but not in humans or other flora, have to be identified. The alternative oxidase (AOX) is one such possibility. This enzyme is insensitive to cyanide, but is sensitive to compounds such as salicylhydroxamic acid (SHAM), disulfiram and n-alkyl gallates. The growth each of six Candida spp. was inhibited significantly by ~13 mM SHAM or 2 mM cyanide, albeit to differing extents. In C. dubliniensis, C. krusei and C. tropicalis the rate of O2 uptake was inhibited by 18-36% by 25 mM SHAM, but this had little or no effect on C. glabrata, C. guilliermondii or C. parapsilosis. Although SHAM substantially inhibited the growth of Candida spp., it is unlikely that the inhibition of AOX was the cause. Salicylhydroxamic acid is used therapeutically in the treatment of urinary tract infections and urolithiasis, but it also has some potential in the treatment of Candida spp. infection.
Abstract: The potential of entomopathogenic nematodes in suppressing T. squalida population on cauliflower from transplanting to harvest was evaluated. Significant reductions in plant infestation percentage and population density (/m2) were recorded throughout the plantation seasons, 2011 and 2012 before and after spraying the plants. The percent reduction in numbers/m2 was the highest in March for the treatments with Heterorhabditis indica Behera and Heterorhabditis bacteriophora Giza during the plantation season 2011, while at the plantation season 2012, the reduction in population density was the highest in January for Heterorhabditis Indica Behera and in February for H . bacteriophora Giza treatments. In a comparison test with conventional insecticides Hostathion and Lannate, there were no significant differences in control measures resulting from treatments with H. indica Behera, H. bacteriophora Giza and Lannate. At the plantation season is 2012. Also, the treatments reduced the economic threshold of T. squalida on cauliflower in this experiment as compared with before and after spraying with both the two entomopathogenic nematodes at both seasons 2011 and 2012. This means an increase in the marketability of heads harvested as a consequence of monthly treatments.
Abstract: Sickle cell anemia is a recessive genetic disease
caused by the presence in the red blood cell, of abnormal hemoglobin
called hemoglobin S. It results from the replacement in the beta chain
of the acid glutamic acid by valin at position 6. Topics may be
homozygous (SS) or heterozygous (AS) most often
asymptomatic. Other mutations result in compound heterozygous:
- Synthesis of hemoglobin C mutation in the sixth leucin codon
(heterozygous SC);
- ß-thalassemia (heterozygous S-ß thalassemia).
SS homozygous, heterozygous SC and S- ß -thalassemia are grouped
under the major sickle cell syndromes.
To make a laboratory diagnosis of hemoglobinopathies in a
portion of the population in region of Batna, our study was
conducted on 115 patients with suspected sickle cell anemia, all cases
have benefited from hematological tests as blood count (count RBC,
calculated erythrocyte indices, MCV and MCHC, measuring the
hemoglobin concentration) and a biochemical test in this case
electrophoresis CAPILLARYS HEMOGLOBIN (E).
The results showed:
27 cases of sickle cell anemia were found on 115 suspected cases,
73,03% homozygous sickle cell disease and 59,25% sickle cell trait.
Finally, the double heterozygous S/C, represent the incidence rate of
3, 70%.
Abstract: Toxoplasma gondii is an intracellular parasite capable
of infecting all nucleated cells in a diverse array of species.
Toxoplasma plaque assay have been described using Bacto Agar.
Because of its experimental advantages carboxymethyl cellulose
overlay, medium viscosity was choosing and the aim of this work
was to develop alternative method for formation of T. gondii plaques.
Tachyzoites were inoculated onto monolayers of Vero cells and
cultured at 37° C under 5 % CO2. The cultures were followed up by
microscopy inspection. Small plaques were visible by naphtol blue
stain 4 days after infection. Larger plaques could be observed by day
10 of culture. The carboxymethyl cellulose is a cheap reagent and the
methodology is easier, faster than assays under agar overlay. This is
the first description of the carboxymethyl cellulose overlay use for
obtaining the formation of T. gondii plaques and may be useful in
consequent obtaining tachyzoites for detailed studies.