Abstract: Chitosan is a natural polysaccharide prepared by the N-deacetylation of chitin. In this study, the physicochemical and antibacterial properties of chitosan nanoparticles, produced by ultrasound irradiation, were evaluated. The physicochemical properties of the nanoparticles were determined by dynamic light scattering and zeta potential analysis. Chitosan nanoparticles inhibited the growth of E. coli. The minimum inhibitory concentration (MIC) values were lower than 0.5 mg/mL, and the minimum bactericidal concentration (MBC) values were similar or higher than MIC values. Confocal laser scanning micrographs (CLSM) were used to observe the interaction between E. coli suspensions mixed with FITC-labeled chitosan polymers and nanoparticles.
Abstract: The Oscillatory electroosmotic flow (OEOF) in power
law fluids through a microchannel is studied numerically. A
time-dependent external electric field (AC) is suddenly imposed
at the ends of the microchannel which induces the fluid motion.
The continuity and momentum equations in the x and y direction
for the flow field were simplified in the limit of the lubrication
approximation theory (LAT), and then solved using a numerical
scheme. The solution of the electric potential is based on the
Debye-H¨uckel approximation which suggest that the surface potential
is small,say, smaller than 0.025V and for a symmetric (z : z)
electrolyte. Our results suggest that the velocity profiles across
the channel-width are controlled by the following dimensionless
parameters: the angular Reynolds number, Reω, the electrokinetic
parameter, ¯κ, defined as the ratio of the characteristic length scale
to the Debye length, the parameter λ which represents the ratio
of the Helmholtz-Smoluchowski velocity to the characteristic length
scale and the flow behavior index, n. Also, the results reveal that
the velocity profiles become more and more non-uniform across the
channel-width as the Reω and ¯κ are increased, so oscillatory OEOF
can be really useful in micro-fluidic devices such as micro-mixers.
Abstract: Coatings based on polyaniline (PANI) can improve the resistance of steel against corrosion. In this work, the preparation of stable suspensions of colloidal PANI-SiO2 particles, suitable for obtaining of composite anticorrosive coating on steel, is described. Electrokinetic data as a function of pH are presented, showing that the zeta potentials of the PANI-SiO2 particles are governed primarily by the charged groups at the silica oxide surface. Electrosteric stabilization of the PANI-SiO2 particles’ suspension against aggregation is realized at pH>5.5 (EB form of PANI) by adsorption of positively charged polyelectrolyte molecules onto negatively charged PANI-SiO2 particles. The PANI-SiO2 particles are incorporated by electrodeposition into the metal matrix of zinc in order to obtain composite (hybrid) coatings. The latter are aimed to ensure sacrificial protection of steel mainly in aggressive media leading to local corrosion damages. The surface morphology of the composite zinc coatings is investigated with SEM. The influence of PANI-SiO2 particles on the cathodic and anodic processes occurring in the starting electrolyte for obtaining of the coatings is followed with cyclic voltammetry. The electrochemical and corrosion behavior is evaluated with potentiodynamic polarization curves and polarization resistance measurements. The beneficial effect of the stabilized PANI-SiO2 particles for the increased protective ability of the composites is commented and discussed.
Abstract: This paper sets to demonstrate a modeling of electrokinetic mixing employing electroosmotic stationary and time-dependent microchannel using alternate zeta patches on the lower surface of the micromixer in a lab on chip microfluidic device. Electroosmotic flow is amplified using different 2D and 3D model designs with alternate and geometric zeta potential values such as 25, 50, and 100 mV, respectively, to achieve high concentration mixing in the electrokinetically-driven microfluidic system. The enhancement of electrokinetic mixing is studied using Finite Element Modeling, and simulation workflow is accomplished with defined integral steps. It can be observed that the presence of alternate zeta patches can help inducing microvortex flows inside the channel, which in turn can improve mixing efficiency. Fluid flow and concentration fields are simulated by solving Navier-Stokes equation (implying Helmholtz-Smoluchowski slip velocity boundary condition) and Convection-Diffusion equation. The effect of the magnitude of zeta potential, the number of alternate zeta patches, etc. are analysed thoroughly. 2D simulation reveals that there is a cumulative increase in concentration mixing, whereas 3D simulation differs slightly with low zeta potential as that of the 2D model within the T-shaped micromixer for concentration 1 mol/m3 and 0 mol/m3, respectively. Moreover, 2D model results were compared with those of 3D to indicate the importance of the 3D model in a microfluidic design process.
Abstract: Inulinase from Aspergillus niger was covalently immobilized on magnetic nanoparticles (MNPs/Fe3O4) covered with soy protein isolate (SPI/Fe3O4) functionalized by bovine serum albumin (BSA) nanoparticles. MNPs are promising enzyme carriers because they separate easily under external magnetic fields and have enhanced immobilized enzyme reusability. As MNPs aggregate simply, surface coating strategy was employed. SPI functionalized by BSA was a suitable candidate for nanomagnetite coating due to its superior biocompatibility and hydrophilicity. Fe3O4@SPI-BSA nanoparticles were synthesized as a novel carrier with narrow particle size distribution. Step by step fabrication monitoring of Fe3O4@SPI-BSA nanoparticles was performed using field emission scanning electron microscopy and dynamic light scattering. The results illustrated that nanomagnetite with the spherical morphology was well monodispersed with the diameter of about 35 nm. The average size of the SPI-BSA nanoparticles was 80 to 90 nm, and their zeta potential was around −34 mV. Finally, the mean diameter of fabricated Fe3O4@SPI-BSA NPs was less than 120 nm. Inulinase enzyme from Aspergillus niger was covalently immobilized through gluteraldehyde on Fe3O4@SPI-BSA nanoparticles successfully. Fourier transform infrared spectra and field emission scanning electron microscopy images provided sufficient proof for the enzyme immobilization on the nanoparticles with 80% enzyme loading.
Abstract: During the recent years the six-fold growth of cancer in Iran has led the production of healthy products to become a challenge in the food industry. Due to the young population in the country, the consumption of fast foods is growing. The chemical cancer-causing preservatives are used to produce these products more than the standard; so using an appropriate alternative seems to be important. On the one hand, the plant essential oils show the high antimicrobial potential against pathogenic and spoilage microorganisms and on the other hand they are highly volatile and decomposed under the processing conditions. The study aims to produce the loaded chitosan nanoparticles with different concentrations of savory essential oil to improve the anti-microbial property and increase the resistance of essential oil to oxygen and heat. The encapsulation efficiency was obtained in the range of 32.07% to 39.93% and the particle size distribution of the samples was observed in the range of 159 to 210 nm. The range of Zeta potential was obtained between -11.9 to -23.1 mV. The essential oil loaded in chitosan showed stronger antifungal activity against Rhizopus stolonifer. The results showed that the antioxidant property is directly related to the concentration of loaded essential oil so that the antioxidant property increases by increasing the concentration of essential oil. In general, it seems that the savory essential oil loaded in chitosan particles can be used as a food processor.
Abstract: In this study, encapsulation of agarwood oil with non-ionic surfactant, Tween 80 was prepared at critical micelle concentration of 0.0167 % v/v to produce the most stable nano-emulsion in aqueous. The encapsulation has minimized the bioactive compounds degradation in various pH conditions thus prolong their shelf life and maintained its initial oil grade. The oil grading of the prepared samples were conducted using the gel electrophoresis instead of using common analytical industrial grading such as gas chromatography- mass spectrometry (GC- MS). The grading method was chosen due to their unique zeta potential value after the encapsulation process. This paper demonstrates the feasibility of applying the electrophoresis principles to separate the encapsulated agarwood oil or grading of the emulsified agarwood oil. The results indicated that the grading process are potential to be further investigate based on their droplet size and zeta potential value at various pH condition when the droplet were migrate through polyacrylamide gel.
Abstract: Surface characteristics of Bacillus megaterium strain
were investigated; zeta potential, FTIR and contact angle were
measured. Surface energy components including Lifshitz-van der
Waals, Hamaker constant, and acid/base components (Lewis
acid/Lewis base) were calculated from the contact angle data. The
results showed that the microbial cells were negatively charged over
all pH regions with high values at alkaline region. A hydrophilic
nature for the strain was confirmed by contact angle and free energy
of adhesion between microbial cells. Adsorption affinity of the strain
toward dolomite was studied at different pH values. The results
showed that the cells had a high affinity to dolomite at acid pH
comparing to neutral and alkaline pH. Extended DLVO theory was
applied to calculate interaction energy between B. megaterium cells
and dolomite particles. The adsorption results were in agreement with
the results of Extended DLVO approach. Surface changes occurred
on dolomite surface after the bio-treatment were monitored; contact
angle decreased from 69° to 38° and the mineral’s floatability
decreased from 95% to 25% after the treatment.
Abstract: Electrostatic interaction energy (ΔEEDL) is a part of the Extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory, which, together with van der Waals (ΔEVDW) and acid base (ΔEAB) interaction energies, has been extensively used to investigate the initial adhesion of bacteria to surfaces. Electrostatic or electrical double layer interaction energy is considerably affected by surface potential; however it cannot be determined experimentally and is usually replaced by zeta (ζ) potential via electrophoretic mobility. This paper focusses on the effect of ionic concentration as a function of pH and the effect of mineral grain size on ζ potential. It was found that both ionic strength and mineral grain size play a major role in determining the value of ζ potential for the adhesion of P. putida to hematite and quartz surfaces. Higher ζ potential values lead to higher electrostatic interaction energies and eventually to higher total XDLVO interaction energy resulting in bacterial repulsion.
Abstract: Preparation of nanoparticles of cerium oxide and
adsorption of bovine serum albumin on them were studied. Particle
size distribution and influence of pH on zeta potential of prepared
CeO2 were determined. Average size of prepared cerium oxide
nanoparticles was 9 nm. The simultaneous measurements of the
bovine serum albumin adsorption and zeta potential determination of
the (adsorption) suspensions were carried out. The adsorption
isotherms were found to be of typical Langmuir type; values of the
bovine serum albumin adsorption capacities were calculated.
Increasing of pH led to decrease of zeta potential and decrease of
adsorption capacity of cerium oxide nanoparticles. The maximum
adsorption capacity was found for strongly acid suspension (am =
118 mg/g). The samples of nanoceria with positive zeta potential
adsorbed more bovine serum albumin on the other hand, the samples
with negative zeta potential showed little or no protein adsorption.
Surface charge or better say zeta potential of CeO2 nanoparticles
plays the key role in adsorption of proteins on such type of materials.
Abstract: In this paper, snow samples containing dust particles
from several sampling points around the city of Ostrava were
analyzed. The pH values of sampled snow were measured and solid
particles analyzed. Particle size, zeta potential and content of selected
heavy metals were determined in solid particles. The pH values of
most samples lay in the slightly acid region. Mean values of particle
size ranged from 290.5 to 620.5 nm. Zeta potential values varied
between -5 and -26.5 mV. The following heavy metal concentration
ranges were found: copper 0.08-0.75 mg/g, lead 0.05-0.9 mg/g,
manganese 0.45-5.9 mg/g and iron 25.7-280.46 mg/g. The highest
values of copper and lead were found in the vicinity of busy
crossroads, and on the contrary, the highest levels of manganese and
iron were detected close to a large steelworks. The proportion
between pH values, zeta potentials, particle sizes and heavy metal
contents was established. Zeta potential decreased with rising pH
values and, simultaneously, heavy metal content in solid particles
increased. At the same time, higher metal content corresponded to
lower particle size.
Abstract: Solid lipid nanoparticles (SLNs) have gained great attention for the topical treatment of skin associated fungal infection as they facilitate the skin penetration of loaded drugs. Our work deals with the preparation of nystatin loaded solid lipid nanoparticles (NystSLNs) using the hot homogenization and ultrasonication method. The prepared NystSLNs were characterized in terms of entrapment efficiency, particle size, zeta potential, transmission electron microscopy, differential scanning calorimetry, rheological behavior and in vitro drug release. A stability study for 6 months was performed. A microbiological study was conducted in male rats infected with Candida albicans, by counting the colonies and examining the histopathological changes induced on the skin of infected rats. The results showed that SLNs dispersions are spherical in shape with particle size ranging from 83.26±11.33 to 955.04±1.09 nm. The entrapment efficiencies are ranging from 19.73±1.21 to 72.46±0.66% with zeta potential ranging from -18.9 to -38.8 mV and shear-thinning rheological Behavior. The stability studies done for 6 months showed that nystatin (Nyst) is a good candidate for topical SLN formulations. A least number of colony forming unit/ ml (cfu/ml) was recorded for the selected NystSLN compared to the drug solution and the commercial Nystatin® cream present in the market. It can be fulfilled from this work that SLNs provide a good skin targeting effect and may represent promising carrier for topical delivery of Nyst offering the sustained release and maintaining the localized effect, resulting in an effective treatment of cutaneous fungal infection.
Abstract: Compatibility between sulfonated acetone- formalehyde superplasticizer (SAF) and copolymer-based grinding aids (GA) were studied by fluidity, Zeta potential, setting time of cement pasts, initial slump and slump flow of concrete and compressive strength of concrete. ESEM, MIP, and XRD were used to investigate the changing of microstructure of interior concrete. The results indicated that GA could noticeably enhance the dispersion ability of SAF. It was found that better fluidity and slump-keeping ability of cement paste were obtained in the case of GA. In addition, GA together with SAF had a certain retardation effect on hydration of cement paste. With increasing of the GA dosage, the dispersion ability and retardation effect of admixture increased. The compressive strength of the sample made with SAF and GA after 28 days was higher than that of the control sample made only with SAF. The initial slump and slump flow of concrete increased by 10.0% and 22.9%, respectively, while 0.09 wt.% GA was used. XRD examination indicated that new products were not found in the case of GA. In addition, more dense arrangement of hydrates and lower porosity of the specimen were observed by ESEM and MIP, which contributed to higher compressive strength.
Abstract: The main aim of the present research was to encapsulate mefenamic acid in niosomes andincorporate the prepared niosomes in the carbopol gel base for sustained therapeutic action. Mefenamic acid loaded niosomes were prepared by thin film hydration technique and evaluated for entrapment efficiency, vesicular size and zeta potential. The entrapment efficiency of the prepared niosomes was found to increase with decreasing the HLB values of surfactants and vesicle size was found to increase with increasing the cholesterol concentration. Niosomal vesicles with good entrapment efficiencies were incorporated in carbopol gel base to form the niosomal gel. The prepared niosomal gel was evaluated for pH, viscosity, spreadability, extrudability and skin permeation study across the rat skin. The results of permeation study revealed that the gel formulated with span 60 niosomes sustained the drug release for 12h. Further the in vivo study showed the good inhibition of inflammation by the gel prepared with span 60 niosomes.
Abstract: Metal working fluids were used in the preparation of oil in water emulsions. The size of oil droplets were evaluated by using the analysis of video images taken from the zeta potential measurements. The evaluated size distributions for emulsions were also tested by microscopic analysis. In addition, emulsion stabilities were discussed depending on electrolyte concentration and pH. The results showed that the stability of oil emulsions was strongly related to pH and the concentration of CaCl2. However, the same dependency was not observed for NaCl.
Abstract: This study was conducted to formulate diclofenac sodium-loaded chitosan nanoparticles and to study the effect of formulation compositions on particle size and zeta potential of chitosan nanoparticles (CSN) containing diclofenac sodium (DC) prepared by ionotropic gelation method. It was found that the formulations containing chitosan, DC and tripolyphosphate (TPP) at a weight ratio of 4:1:1, respectively, with various pH provided various systems. At pH 5.0 and 6.0, the obtained systems were turbid because of precipitation of DC and chitosan, respectively. However, the dispersed system of CSN possessing diameter of 108±1 nm and zeta potential of 19±1 mV could be obtained at pH 5.5. These CSN also showed spherical morphology observed via a transmission scanning electron microscope. Change in weight ratio of chitosan:DC:TPP i.e. 1:1:1, 2:1:1, 3:1:1 and 4:1:1 showed that these ratios led to precipitation of particles except for the ratio of 4:1:1 providing CSN properly. The effect of Tween 80 as a stabilizer was also determined. It suggested that increment of Tween 80 concentration to 0.02% w/v could stabilize CSN at least 48 hours. However, increment of Tween 80 to 0.03% w/v led to quick precipitation of particles. The study of effect of TPP suggested that increment of TPP concentration increased particle size but decreased zeta potential. The excess TPP caused precipitation of CSN. Therefore, the optimized CSN was the CSN containing chitosan, DC and TPP at the ratio of 4:1:1and 0.02% w/v Tween 80 prepared at pH 5.5. Their particle size, zeta potential and entrapment efficiency were 128±1 nm, 15±1 mV and 45.8±2.6%, respectively.
Abstract: The electrochemical coagulation of a kaolin
suspension was investigated at the currents of 0.06, 0.12, 0.22, 0.44,
0.85 A (corresponding to 0.68, 1.36, 2.50, 5.00, 9.66 mA·cm-2,
respectively) for the contact time of 5, 10, 20, 30, and 50 min. The
TSS removal efficiency at currents of 0.06 A, 0.12 A and 0.22 A
increased with the amount of iron generated by the sacrificial anode,
while the removal efficiencies did not increase proportionally with
the amount of iron generated at the currents of 0.44 and 0.85 A,
where electroflotation was clearly observed. Zeta potential
measurement illustrated the presence of the highly positive charged
particles created by sorption of highly charged polymeric metal
hydroxyl species onto the negative surface charged kaolin particles at
both low and high applied currents. The disappearance of the
individual peaks after certain contact times indicated the attraction
between these positive and negative charged particles causing
agglomeration. It was concluded that charge neutralization of the
individual species was not the only mechanism operating in the
electrocoagulation process at any current level, but electrostatic
attraction was likely to co-operate or mainly operate.
Abstract: In the present study, development of salbutamol
sulphate nanoparticles that adhere to gastric mucus was investigated.
Salbutamol sulphate has low bioavailability due to short transit time in
gastric. It also has a positive surface charge that provides hurdles to be
encapsulated by the positively strong mucoadhesive polymer of
chitosan. To overcome the difficulties, the surface charge of active
ingredient was modified using several nonionic and anionic
stomach-specific polymers. The nanoparticles were prepared using
ionotropic gelation technique. The evaluation involved determination
of particle size, zeta potential, entrapment efficiency, in vitro drug
release and in vitro mucoadhesion test. Results exhibited that the use
of anionic alginate polymer was more satisfactory than that of
nonionic polymer. Characteristics of the particles was nano-size, high
encapsulation efficiency, fulfilled the drug release requirements and
adhesive towards stomach for around 11 hours. This result shows that
the salbutamol sulphate nanoparticles can be utilized for improvement
its delivery.
Abstract: The study investigates the mixing performance of
electrokinetically-driven power-law fluids in a microchannel
containing patterned trapezoid blocks. The effects of the geometry
parameters of the patterned trapezoid blocks and the flow behavior
index in the power-law model on the mixing efficiency within the
microchannel are explored. The results show that the mixing efficiency
can be improved by increasing the width of the blocks and extending
the length of upper surface of the blocks. In addition, the results show
that the mixing efficiency increases with an increasing flow behavior
index. Furthermore, it is shown that a heterogeneous patterning of the
zeta potential on the upper surfaces of the trapezoid blocks prompts
the formation of local flow recirculations, and therefore improves the
mixing efficiency. Consequently, it is shown that the mixing
performance improves with an increasing magnitude of the
heterogeneous surface zeta potential.
Abstract: The present study is aim to prepare and evaluate the selfnanoemulsifying drug delivery (SNEDDS) system of a poorly water soluble drug valsartan in order to achieve a better dissolution rate which would further help in enhancing oral bioavailability. The present research work describes a SNEDDS of valsartan using labrafil M 1944 CS, Tween 80 and Transcutol HP. The pseudoternary phase diagrams with presence and absence of drug were plotted to check for the emulsification range and also to evaluate the effect of valsartan on the emulsification behavior of the phases. The mixtures consisting of oil (labrafil M 1944 CS) with surfactant (tween 80), co-surfactant (Transcutol HP) were found to be optimum formulations. Prepared formulations were evaluated for its particle size distribution, nanoemulsifying properties, robustness to dilution, self emulsication time, turbidity measurement, drug content and invitro dissolution. The optimized formulations are further evaluated for heating cooling cycle, centrifugation studies, freeze thaw cycling, particle size distribution and zeta potential were carried out to confirm the stability of the formed SNEDDS formulations. The prepared formulation revealed t a significant improvement in terms of the drug solubility as compared with marketed tablet and pure drug.