Abstract: Using effluent as a sustainable water resource for agriculture not only could provide part of water needs but also would save the existing water resources, durably. Vinasse, the effluent of ethanol alcohol factories, a by-product, which is derived from sugarcane molasses, is one of the water resources that could be effectively utilized for agricultural purposes. In the present study in order to investigate the application of different ratios of water: vinasse on germination and growth of barley seedlings an experiment was designed in pots with completely randomized design with three replications and control treatment. The consequences of four irrigation levels were studied with different water: effluent ratios (100% water, 90% water & 10% effluent, 75% water & 25% effluent, 50% water & 50% effluent) on germination and growth of barley seedling components in sandy-loam soil. The results showed that, with increasing the percentage of vinasse in the irrigation admixture, the germination percentage in barley seedlings decreased, significantly, so that the decrease in germination in comparison with the control samples in the second and third treatments was 20% and 93.33%, respectively. Seed germination percentage was about 46.66. The average stem length in seedlings was 14.3 mm and the average root length was 9.37 mm. The averages of the soils Electrical Conductivity (EC) and pH which were under irrigation with different ratios of vinasse (dSm-1) were 5.85 and 7.32, respectively, which showed a 76.2% increase in soil salinity.
Abstract: In this study, raw coffee wastewater (CWW) was used as a complex substrate for anaerobic digestion. The inoculum adaptation stage, microbial diversity analysis and biomethane potential (BMP) tests were performed. A fast inoculum adaptation stage was used by the replacement of vinasse to CWW in an anaerobic sequential batch reactor (AnSBR) operated at mesophilic conditions. Illumina MiSeq sequencing was used to analyze the microbial diversity. While, BMP tests using inoculum adapted to CWW were carried out at different inoculum to substrate (I/S) ratios (2:1, 3:1 and 4:1, on a VS basis). Results show that the adaptability percentage was increased gradually until it reaches the highest theoretical value in a short time of 10 d; with a methane yield of 359.10 NmL CH4/g COD-removed; Methanobacterium beijingense was the most abundant microbial (75%) and the greatest specific methane production was achieved at I/S ratio 4:1, whereas the lowest was obtained at 2:1, with BMP values of 320 NmL CH4/g VS and 151 NmL CH4/g VS, respectively. In conclusion, gradual replacement of substrate was a feasible method to adapt the inoculum in a short time even using complex raw substrates, whereas in the BMP tests, the specific methane production was proportional to the initial amount of inoculum.